LOCUS       Exported               12731 bp ds-DNA     circular SYN 20-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Sho Yoshimatsu
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 10, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12731
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    14..47
                     /label=loxP
     misc_feature    76..649
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             666..1382
                     /codon_start=1
                     /label=EGFPdelSTOP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1407..1472
                     /codon_start=1
                     /label=F2A
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             1479..2273
                     /codon_start=1
                     /label=NeoR/KanR
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    2291..2324
                     /label=lox2272
     misc_feature    2347..7753
                     /label=actB3'arm
     misc_feature    2382..2387
                     /label=polyAsignal
     misc_feature    5049..5054
                     /label=polyAsignal
     misc_feature    5053..5058
                     /label=polyAsignal
     promoter        complement(7785..7803)
                     /label=T7 promoter
     primer_bind     complement(7810..7826)
                     /label=M13 fwd
     CDS             7964..8266
                     /codon_start=1
                     /label=ccdB
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             8615..9409
                     /codon_start=1
                     /label=NeoR/KanR
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9616..9990
                     /codon_start=1
                     /label=BleoR
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      10128..10716
                     /direction=RIGHT
                     /label=ori
     protein_bind    11004..11025
                     /label=CAP binding site
     promoter        11040..11070
                     /label=lac promoter
     protein_bind    11078..11094
                     /label=lac operator
     primer_bind     11102..11118
                     /label=M13 rev
     promoter        11136..11154
                     /label=SP6 promoter
     misc_feature    11191..12731
                     /label=actB5'arm
     CDS             join(11191..11303,11746..12184,12278..12459,12570..12713)
                     /codon_start=1
                     /label=ACTB
                     /translation="IWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT
                     PAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDY
                     LMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQV
                     ITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMY
                     PGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDES
                     GPSIVHRKCF"
     misc_feature    11191..11303
                     /label=Exon2
     misc_feature    11746..12184
                     /label=Exon3
     misc_feature    12278..12459
                     /label=Exon4
     misc_feature    12570..12713
                     /label=last Exon
ORIGIN
        1 aattcgccct tggataactt cgtatagcat acattatacg aagttataga tcttcccttt
       61 tttttccaca gtccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
      121 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
      181 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
      241 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
      301 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
      361 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
      421 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
      481 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
      541 tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
      601 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag gatctgctag
      661 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      721 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      781 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      841 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
      901 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      961 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1021 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1081 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     1141 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     1201 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1261 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1321 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1381 agaggcctcg cgctaagcgg gcaccggtga aacagacttt gaattttgac cttctcaagt
     1441 tggcgggaga cgtggagtcc aacccagggc cggatatcat gattgaacaa gatggattgc
     1501 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     1561 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     1621 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     1681 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     1741 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     1801 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     1861 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     1921 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     1981 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     2041 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     2101 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     2161 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     2221 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcggccg
     2281 gccgctcgac ataacttcgt ataggatact ttatacgaag ttatgatatc catcacactg
     2341 gcggccgcgg gcttacctgt acactgactt gagaccagtt gaataaagtg cacaccttaa
     2401 aaatgaggcc aagcatgact ttgtgtcggg ctggggtgtg ggcagcaggt gaacccaggg
     2461 gaagggtggg gcagtggggg ccagcctgtc cctactgagt ggaggtgggc tgaggttctc
     2521 tctccttgac attgagcagc cttagaatgt gagggaagtt taagattcag gcctctgctc
     2581 attggggagt tcctggcttg gccctgccac ctgtccctag gtaccccagt ttctctgggt
     2641 tcatccagaa ggtctagatg ctcaggaagt gctggttggc acctgtgttt cttaggagtg
     2701 gagatccctt agggtcactc cgggcagggg gttcttgagt gggagtgtag tgggtgttcc
     2761 tagctgccat gcctttgtcc tgctcactgt attggagtgg agtcgtccat aggctgagag
     2821 aagccatgac tcctgcttct ctacccagac agggaaatct gggatcacag tcgaattgtg
     2881 gtgggggatc catggggcca tgtggtggca ggcagctctg gttagctctg gactgagctg
     2941 gattctgcct tgtgacgagt aggcgccatc ctgtggcctc tcagggccca gtttgtgacc
     3001 catgctgcag agggagatgg ccagactcct ggacagcagg gcatgcctgg cattgcaggt
     3061 cagattcttt tttttttgag atggggtctg gctttgttgc tcaggcttta gttcagtggt
     3121 acaaccacag ctcactgcag cctcagcctt ctgggcaagc agttctgcct cagcctccca
     3181 aatagctgga actacacagg cacacccagc tagttgtggg tcagattctc acttgtgcag
     3241 agctgagtca cttttgaaag aaacaaaacc tggcttccct tccgctgcta gaagctttga
     3301 ttgatagtag cggagaatga aggaaggaga cgaggctccc ttgcggggat accctttacc
     3361 aaaaacacca cctggtgtat cacagttgtt ctggaaatga tctggtcccc aggaggcctg
     3421 gactgcctct cacttgtgca cagtggctgc tggcagagag gaaggttgtc cctttctgtg
     3481 ttatcttgga ggttccccga agggtctgcc aagagcagtt ctagctcttt tggtctttac
     3541 tgagtgacca ctagatggtg ggcctgaagc tactgagatg acctggacaa gaaccctgcc
     3601 ttggaggagc tgtcaatctg gaaatgagga cttcattcat ttgttcattc cttgatccat
     3661 ttctccattg aatcactatt tgtgctatgt actgtgggta tggaggagaa ccaaacagat
     3721 gtgttcctgt cctcaccagg cacccaattt aaaggggaag acatcataaa cacaaaggta
     3781 cacaaatgag tgtccaatga cacggtgtca tcatgggaag aaaaagcaga ggctttgatg
     3841 aacacgatca ttaggtgata ttcattatgt tgattgtggt ggtctagaca taaattaaca
     3901 cttatctagg ctgggcgtgg tggctcatgc ctgtaatccc agcactttgg gaggctgagg
     3961 tgggtggatc aagagatcga gaccatcctg gccaacatag tgaaacccca tctttactaa
     4021 aaatacaaaa attagctggg cgtggtggtg cacacttggg aggctgaggt aggagaactg
     4081 cttgaactgg ggaggcgaac gttgcagtga gctaagattg tgctactgta ctccagcctg
     4141 gtgctagcaa cactgtctta aaaaaaaaca cttacctaat tgaccctgag gtgatggccc
     4201 attcctgtaa tcccagcact ttgggaagct gaggtgggag gatggcttga gccaggagct
     4261 caaggttgct gtgagctatg attgctctat cgcactccat cctgggtgac agaacaagac
     4321 ccagactcta aaaactaaca taggacaggc agagcatggt ggctcgcagc actttgggag
     4381 gctgaggcag gtagctcatg tgagcccaaa tgaggccagc ctgggcagca tagtgagatc
     4441 ctgtctcaat ttttttttga gatggaatct cctgttaccc aggctggagt gcagtgtcca
     4501 aatctccact cactgcaacc tccatctccc aggttcaagt gattcttctg ccttagcctc
     4561 ttaaatagct gggactacag gtacccaccg ctacgtctgt acaatttttg gatttttagt
     4621 agaaacgggg tttcactatg tcgcctaggc tggttttgaa ctcctgacct catgattcac
     4681 ctgcctcagc ctcccaaagt tctgggatta cagacttgaa ccactgtgcc tggcctcatt
     4741 ttttttgaga caaaatttta ctcgtttccc aggctggagt gcaatggcgt gatctcggtt
     4801 cactgcaacc tcttgcctcc tggattcaag tgattctcct gcctcagcct ccagaatagc
     4861 tgggattatg ggcacttgcc accatgccca gctaatttta catgtttagt agagacaggg
     4921 tttctccatg ttggtgaggc tggtctcaaa ctcctgacct caggtgatcc acccttcttg
     4981 gcctccaaaa gtgttgggat tacaagcatg agctaccaca cccactctca atttttaaat
     5041 agttaaaaaa taaataaaat tatgaaattg taccacagta agactagaaa aaaataagaa
     5101 atataggggg ggttttgttt attttatttt atttaattca gagtcttact ctgtccccag
     5161 gctggagtgc aatggcacga tctcagctca ctgcaacccc tgcctccctg ttcaagtaat
     5221 tctcctgcct cagtctcctg agtagctggg actacaggca tctgctacca tacccagcta
     5281 atttttgtat ttttagtaaa gacacggttt caccatgttg gccaggctag tctcaaactc
     5341 ctgacttaaa gtgattcacc tgtctcccaa aatcctggga ttgcaggttt gagccaccat
     5401 gcctggccag atttttaatt ttttaattta tttttttaga gacacaggtc tcactgtgtt
     5461 gcctaggctg gagtgcagtg gtgaggttac agctcattgc agcctccaat ttatagactc
     5521 aagtgatcct cccacctcag ctgccccaaa atgctgggga gctatcatgc ctggccagat
     5581 tttaaattgt ggggaccacc aattttgtgt ttaattgatg tcgcaatgct tgggccttga
     5641 ccacgtcctc ctgcatgggt cctgcttagc tttcctccac tgcggctcag tctgcagttt
     5701 ctcctggtgc ttgctgagcc agctgtgcat ggcacatgtt tttcattttt tctttttatt
     5761 ttttgagaca gagtctcgct ctgttaccca ggctgggaat agagtggtac aatctcggct
     5821 cactgcaacc tctgcctcct gggttcaagt gattctcctg cctcagcctc ctgagtagct
     5881 ggaactacag gtgcctggcc acaagggccc aggctaaatt ttatattttt agtagagaca
     5941 gggtttcgcc atgctggcca ggctggtctt caaagtcctt tacctcatga tccgctgcct
     6001 cagcctccca aagtgctgag atcacactca tgagcactgt gcctggcctt tttttttttt
     6061 tttcctccca gacagagtgt gctctgctgc caggctggag tgcgtggcgt gatctcggct
     6121 cactgcaagc tctgcctctt gggttcaagc agttgtcctg cctcagtctc tgagtagctg
     6181 gaattacagg catgtgccac cgcactcagc taattttttt tttaagttgg aatttggcca
     6241 ggcgcggtgg ctcaagcctg taatcccagc actttgggag gccgaggcag gtggatcatg
     6301 aggtcaagag atctgggtaa tgagcgaaac tccgtctcaa aaaaaaagat ggaattttgc
     6361 tcgttacccc agctggagtg cagtggcact atctcagttc tgcaacctct gcctcctggg
     6421 ttcaatcgat tctcctgcct cagcctccca agtagctggg accacaggcg catgccactg
     6481 tgcctggtta atttttttat ttctcgcaga gacagggtat caccatgttg gccaggctag
     6541 tttcaaactc ttgacctcag gtgatccacc cacctcagcc tcccaaagtg ctgggatcat
     6601 aggcatgagc ctctgcgtcc agtattggtt gtgtgctcct tatgagaatc taatgcccga
     6661 tgatctctca ctgtctccca tcacccccag aagggattgt ctagttgcaa gaaaaccagc
     6721 tcagggctcc cactgattct acatcctggt gagttgtaga attattttat catggccagg
     6781 cacagtggct catgcctata atcccagcac tttgggaggc cgaggtgggt ggatcgcgag
     6841 ggtgggtgac catcctggtc aacaaggtga aaccccatct ctactaaaaa tacaaaaatt
     6901 atctgggcat ggtggtgtgc gcctgtagtc ccagctactc gggaggctga ggcaggagaa
     6961 ttgtttgaac ccaggaggcg gaggttgcgg tgagctgaga tcgtgccatt gcactccagt
     7021 ctgggtaaca acagcgaaac tccatctcaa aaaaaaaaaa ttattttatc atatattaca
     7081 atgtaataat ggaaataaag tatacagtaa gagtaacacg ctgaatcatc ccaaaaccct
     7141 ccccctaccc ccattcgtgg aaaaattgtc ttccacaaaa ctgccaaaaa gattaaggac
     7201 tgctgacatt ggcaacggac agctgggaca gtttggccca taggttgtcc agcagtttca
     7261 gaaactcctg ctccttgcca aagaagttcc agccttaatc tagggcgtgg ctgcacaagt
     7321 ctctgttgcc ggcgcctatt ccaagaaaaa gaggaagagg gccagaaata cagattttaa
     7381 aaagcagagg ggccgggtgc aatggctaat gcctataatc ccagcactct gggagaccga
     7441 ggcagatgga tcacctgagg tcaggagttc aagacagcct ggccaacatg gtgaaaccct
     7501 gtctctacta aaaatacaaa aaattagcca ggcatggtgg catgtgcctg taatccaaac
     7561 tacttgggag gctgaagcag gagaatcact tgaacccggg aggcggaggt tgcagtgagc
     7621 tgagatcgtg ccactgcact ccagcctggg caacagagca agactctgtt tcaaaaaaat
     7681 aaaaaaagca aaggatcatt gaacatgagc agacttgcag acttggcggt agggagagag
     7741 gaactgtcac tctgtcgagc atgcatctag agggcccaat tcgccctata gtgagtcgta
     7801 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7861 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7921 caccgatcgc ccttcccaac agttgcgcag cctatacgta cggcagttta aggtttacac
     7981 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     8041 gccggggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     8101 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     8161 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     8221 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggcatgag
     8281 attatcaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg
     8341 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
     8401 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt
     8461 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
     8521 ctgcaaagta aactggatgg ctttctcgcc gccaaggatc tgatggcgca ggggatcaag
     8581 ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     8641 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     8701 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     8761 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     8821 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     8881 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     8941 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     9001 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     9061 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     9121 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     9181 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     9241 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     9301 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     9361 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttattaacgc
     9421 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     9481 acaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9541 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatagcac
     9601 gtgaggaggg ccaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     9661 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     9721 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     9781 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     9841 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     9901 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     9961 gtgcacttcg tggccgagga gcaggactga cacgtgctaa aacttcattt ttaatttaaa
    10021 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10081 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10141 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10201 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10261 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10321 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10381 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10441 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10501 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10561 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10621 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10681 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10741 cggttcctgg gcttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10801 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10861 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    10921 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    10981 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    11041 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    11101 acaggaaaca gctatgacca tgattacgcc aagctattta ggtgacacta tagaatactc
    11161 aagctatgca tcaagcttgg taccgagctc agatctggca ccacactttt tacaacgagc
    11221 tgcgtgtggc tcctgaggag caccccgtgc tgcttactga ggcccccctg aaccccaagg
    11281 ccaaccgtga gaagatgacc caggtgagtg atctgccacc tcccctggtg gccgcctccc
    11341 tccttcctgg cctcccggag ctgcgccctt tctcactggt tctctcttct gccgttctcc
    11401 gtaggactct cttctctgac ctgagtctcc tttggaactc tgcaggttct atttgctttt
    11461 tcccagatga ggtctttctg gtgtttgcct ttctgactag gtgtctaaaa cagacagtgc
    11521 tgtgggtgta ggtactaaca ctggctcgtg tgacaagccc atgaggctgg tgtaaagcag
    11581 tcttggagtg tgtatacagt aggcgcacag taggtctgaa cagaatcccc atcccaagac
    11641 cccagcacaa ctagcggtgt tcttgcactt actgcatgtc ccctgtctgc tcttggctgt
    11701 ccctagtgac ttcctcagtg tgacacggtg catctctttc tacagatcat gtttgagacc
    11761 ttcaacaccc cagccatgta tgtggccatc caggctgtgc tttccttgta cgcctctggc
    11821 cgtaccactg gcatcgtgat ggactccggt gacggggtca cccacactgt gcccatctat
    11881 gaggggtatg ccctccccca cgccatccta cgtctggacc tggctggccg ggacctgact
    11941 gactacctca tgaagatcct caccgagcgc ggctacagct tcaccaccac agctgagcgg
    12001 gaaatcgtgc gtgacatcaa ggagaagctg tgctacgtcg ccctggactt cgagcaggag
    12061 atggccacgg cggcctccag ctcctccctg gagaagagct atgagctgcc cgatggtcag
    12121 gtcatcacca tcggcaacga gcggttccgc tgccctgagg cactcttcca gccttctttc
    12181 ctgggtaagt ggagaccact gcctagccct gcctgacatg aggttacctt ggggctgtgc
    12241 tgtggaagct taagtctggg tctcatttcc ccctcaggca tggaatcttg tggcatccac
    12301 gaaactacct ttaactccat catgaaatgt gacgtggaca tccgcaaaga cctgtatgcc
    12361 aacacagtgc tgtctggtgg taccaccatg taccctggca tcgctgacag gatgcagaag
    12421 gagatcaccg ccctggcacc cagcacaatg aagatcaagg tgggtgtcct tccagcctga
    12481 gctgacctgg gcagggggca tggggccctg tggtttgtgg ggagctgtca tatcaagggt
    12541 cctcactgcc tgttccctcc actcctcaga tcattgctcc tcccgagcgc aagtactcag
    12601 tgtggattgg tggctccatc ctggcctcgc tgtccacctt ccagcagatg tggatcagca
    12661 agcaggagta tgatgagtcc ggcccctcca tcgtccaccg caaatgcttc taggcggact
    12721 gtgacttagt g
//
