LOCUS       Exported               10023 bp ds-DNA     circular SYN 20-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 10, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
     promoter        143..173
                     /label=lac promoter
     protein_bind    181..197
                     /label=lac operator
     primer_bind     205..221
                     /label=M13 rev
     promoter        239..257
                     /label=SP6 promoter
     misc_feature    294..2749
                     /label=actB5'arm
     misc_feature    839..961
                     /label=Exon1
     misc_feature    1083..1322
                     /label=Exon2
     misc_feature    1764..2202
                     /label=Exon3
     misc_feature    2296..2477
                     /label=Exon4
     misc_feature    2588..2731
                     /label=last Exon
     protein_bind    2763..2796
                     /label=loxP
     misc_feature    join(2821..3303,3305..3398)
                     /label=IRES2
     CDS             3415..4131
                     /codon_start=1
                     /label=EGFPdelSTOP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4156..4221
                     /codon_start=1
                     /label=F2A
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4228..5022
                     /codon_start=1
                     /label=NeoR/KanR
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    5040..5073
                     /label=lox2272
     misc_feature    5096..6892
                     /label=actB3'arm
     misc_feature    5131..5136
                     /label=polyAsignal
     promoter        complement(6911..6929)
                     /label=T7 promoter
     primer_bind     complement(6936..6952)
                     /label=M13 fwd
     CDS             7090..7392
                     /codon_start=1
                     /label=ccdB
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        7603..7652
                     /label=NEOKAN promoter
     CDS             7741..8535
                     /codon_start=1
                     /label=NeoR/KanR
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        8669..8697
                     /label=AmpR promoter
     CDS             8742..9116
                     /codon_start=1
                     /label=BleoR
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      9254..9842
                     /direction=RIGHT
                     /label=ori
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggtcttt
      301 gtccgagccg ggttcttgct cgggtgactg gagggtgggc gcggctgggc cactgccagg
      361 cccagtgggg gctgggctac cattgcgcgt gtgtgctggg gcttgtggcg ctgactgcgc
      421 gtgcgcgctg gagtctaggg cgccgccgct cgggctgggc ggagggcggg ccaggccgga
      481 aggggtgggg tcgccggggc gcaggcgcgc acttcctgcc tgcgccgccg gccgcctgag
      541 ggtgtggccg ctttgcacgc tagtgcgcgc cgaaaccggc gttttttgaa ccgggcggag
      601 gcggggccag cccctggtgg ggagggggcg gaggcctggc ttcctgccgc gcgccgcggg
      661 gccgcctccg accagtgttt gccttttatg gtaataacgc ggccggcccg gcttcctttg
      721 tccccaatcc gggcgcgcgc cggcgccccc tggcggccca aggccgaggc gcgccggaag
      781 tgagtagggc gggggcgacc ccggctcaca gagcgccctg ctcttcccgc agctcaccat
      841 ggatgatgat atcgctgcac ttgttgtcga caacggctcc ggcatgtgca aggccggctt
      901 tgcgggcgac gatgctcccc gggccgtctt cccctctatc gtggggcgcc ccaggcacca
      961 ggtaaggaag ttgagtgggt ggggccgccc caggagcggg cgggaggcga gggcacttag
     1021 gaggtacccc ggcttccggg gtgggggtcg cgcccgtgtt caaagcaccc tgttctttgc
     1081 agggcgtgat ggtgggcatg ggtcagaagg actcctacgt gggtgacgag gcccagagca
     1141 agagaggcat cctgaccctg aagtacccca tcgagcatgg cattgtcacc aactgggacg
     1201 acatggagaa gatctggcac cacacttttt acaacgagct gcgtgtggct cctgaggagc
     1261 accccgtgct gcttactgag gcccccctga accccaaggc caaccgtgag aagatgaccc
     1321 aggtgagtga tctgccacct cccctggtgg ccgcctccct ccttcctggc ctccggagct
     1381 gcgccctttc tcactggttc tctcttctgc cgttctccgt aggactctct tctctgacct
     1441 gagtctcctt tggaactctg caggttctat ttgctttttc ccagatgagg tctttctggt
     1501 gtttgccttt ctgactaggt gtctaaaaca gacagtgctg tgggtgtagg tactaacact
     1561 ggctcgtgtg acaagcccat gaggctggtg taaagcagtc ttggagtgtg tatacagtag
     1621 gcgcacagta ggtctgaaca gaatccccat cccaagaccc cagcacaact agcggtgttc
     1681 ttgcacttac tgcatgtccc ctgtctgctc ttggctgtcc ctagtgactt cctcagtgtg
     1741 acacggtgca tctctttcta cagatcatgt ttgagacctt caacacccca gccatgtatg
     1801 tggccatcca ggctgtgctt tccttgtacg cctctggccg taccactggc atcgtgatgg
     1861 actccggtga cggggtcacc cacactgtgc ccatctatga ggggtatgcc ctcccccacg
     1921 ccatcctacg tctggacctg gctggccggg acctgactga ctacctcatg aagatcctca
     1981 ccgagcgcgg ctacagcttc accaccacag ctgagcggga aatcgtgcgt gacatcaagg
     2041 agaagctgtg ctacgtcgcc ctggacttcg agcaggagat ggccacggcg gcctccagct
     2101 cctccctgga gaagagctat gagctgcccg atggtcaggt catcaccatc ggcaacgagc
     2161 ggttccgctg ccctgaggca ctcttccagc cttctttcct gggtaagtgg agaccactgc
     2221 ctagccctgc ctgacatgag gttaccttgg ggctgtgctg tggaagctta agtctgggtc
     2281 tcatttcccc ctcaggcatg gaatcttgtg gcatccacga aactaccttt aactccatca
     2341 tgaaatgtga cgtggacatc cgcaaagacc tgtatgccaa cacagtgctg tctggtggta
     2401 ccaccatgta ccctggcatc gctgacagga tgcagaagga gatcaccgcc ctggcaccca
     2461 gcacaatgaa gatcaaggtg ggtgtccttc cagcctgagc tgacctgggc agggggcatg
     2521 gggccctgtg gtttgtgggg agctgtcata tcaagggtcc tcactgcctg ttccctccac
     2581 tcctcagatc attgctcctc ccgagcgcaa gtactcagtg tggattggtg gctccatcct
     2641 ggcctcgctg tccaccttcc agcagatgtg gatcagcaag caggagtatg atgagtccgg
     2701 cccctccatc gtccaccgca aatgcttcta ggcggactgt gacttagtga attcgccctt
     2761 ggataacttc gtatagcata cattatacga agttatagat cttccctttt ttttccacag
     2821 tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     2881 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     2941 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     3001 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     3061 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     3121 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     3181 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     3241 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     3301 ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     3361 acggggacgt ggttttcctt tgaaaaacac gatgataagg atctgctagc caccatggtg
     3421 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3481 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3541 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3601 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3661 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3721 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3781 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3841 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3901 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3961 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4021 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4081 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaggcctcgc
     4141 gctaagcggg caccggtgaa acagactttg aattttgacc ttctcaagtt ggcgggagac
     4201 gtggagtcca acccagggcc ggatatcatg attgaacaag atggattgca cgcaggttct
     4261 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     4321 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4381 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     4441 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     4501 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     4561 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     4621 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     4681 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     4741 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     4801 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     4861 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     4921 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     4981 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcggccgg ccgctcgaca
     5041 taacttcgta taggatactt tatacgaagt tatgatatcc atcacactgg cggccgcggg
     5101 cttacctgta cactgacttg agaccagttg aataaagtgc acaccttaaa aatgaggcca
     5161 agcatgactt tgtgtcgggc tggggtgtgg gcagcaggtg aacccagggg aagggtgggg
     5221 cagtgggggc cagcctgtcc ctactgagtg gaggtgggct gaggttctct ctccttgaca
     5281 ttgagcagcc ttagaatgtg agggaagttt aagattcagg cctctgctca ttggggagtt
     5341 cctggcttgg ccctgccacc tgtccctagg taccccagtt tctctgggtt catccagaag
     5401 gtctagatgc tcaggaagtg ctggttggca cctgtgtttc ttaggagtgg agatccctta
     5461 gggtcactcc gggcaggggg ttcttgagtg ggagtgtagt gggtgttcct agctgccatg
     5521 cctttgtcct gctcactgta ttggagtgga gtcgtccata ggctgagaga agccatgact
     5581 cctgcttctc tacccagaca gggaaatctg ggatcacagt cgaattgtgg tgggggatcc
     5641 atggggccat gtggtggcag gcagctctgg ttagctctgg actgagctgg attctgcctt
     5701 gtgacgagta ggcgccatcc tgtggcctct cagggcccag tttgtgaccc atgctgcaga
     5761 gggagatggc cagactcctg gacagcaggg catgcctggc attgcaggtc agattctttt
     5821 ttttttgaga tggggtctgg ctttgttgct caggctttag ttcagtggta caaccacagc
     5881 tcactgcagc ctcagccttc tgggcaagca gttctgcctc agcctcccaa atagctggaa
     5941 ctacacaggc acacccagct agttgtgggt cagattctca cttgtgcaga gctgagtcac
     6001 ttttgaaaga aacaaaacct ggcttccctt ccgctgctag aagctttgat tgatagtagc
     6061 ggagaatgaa ggaaggagac gaggctccct tgcggggata ccctttacca aaaacaccac
     6121 ctggtgtatc acagttgttc tggaaatgat ctggtcccca ggaggcctgg actgcctctc
     6181 acttgtgcac agtggctgct ggcagagagg aaggttgtcc ctttctgtgt tatcttggag
     6241 gttccccgaa gggtctgcca agagcagttc tagctctttt ggtctttact gagtgaccac
     6301 tagatggtgg gcctgaagct actgagatga cctggacaag aaccctgcct tggaggagct
     6361 gtcaatctgg aaatgaggac ttcattcatt tgttcattcc ttgatccatt tctccattga
     6421 atcactattt gtgctatgta ctgtgggtat ggaggagaac caaacagatg tgttcctgtc
     6481 ctcaccaggc acccaattta aaggggaaga catcataaac acaaaggtac acaaatgagt
     6541 gtccaatgac acggtgtcat catgggaaga aaaagcagag gctttgatga acacgatcat
     6601 taggtgatat tcattatgtt gattgtggtg gtctagacat aaattaacac ttatctaggc
     6661 tgggcgtggt ggctcatgcc tgtaatccca gcactttggg aggctgaggt gggtggatca
     6721 agagatcgag accatcctgg ccaacatagt gaaaccccat ctttactaaa aatacaaaaa
     6781 ttagctgggc gtggtggtgc acacttggga ggctgaggta ggagaactgc ttgaactggg
     6841 gaggcgaacg ttgcagtgag ctaagattgt gctactgtac tccagcctgg tgctagaggg
     6901 cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
     6961 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     7021 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagccta
     7081 tacgtacggc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
     7141 gatgtacaga gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt
     7201 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
     7261 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa
     7321 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
     7381 tggggaatat aaatgtcagg catgagatta tcaaaaagga tcttcaccta gatccttttc
     7441 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
     7501 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
     7561 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
     7621 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca
     7681 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc
     7741 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     7801 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     7861 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     7921 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     7981 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     8041 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     8101 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     8161 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     8221 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     8281 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     8341 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     8401 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     8461 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     8521 gacgagttct tctgaattat taacgcttac aatttcctga tgcggtattt tctccttacg
     8581 catctgtgcg gtatttcaca ccgcatacag gtggcacttt tcggggaaat gtgcgcggaa
     8641 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8701 cctgataaat gcttcaataa tagcacgtga ggagggccac catggccaag ttgaccagtg
     8761 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     8821 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     8881 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     8941 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     9001 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     9061 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     9121 tgctaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9181 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9361 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     9421 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9841 aaaacgccag caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca
     9901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    10021 aag
//
