LOCUS       Bxb1Donor_WT_ADRB2_LucR 7662 bp ds-DNA     circular     10-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3461..3610
                     /label="CRE Enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3498..3502
                     /label="CREB half"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3593..3600
                     /label="CREB full"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3524..3610
                     /label="CRE segment"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3472..3479
                     /label="CREB full"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3561..3565
                     /label="CREB half"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    642..710
                     /label="3x FLAG Tag"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3535..3542
                     /label="CREB full"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(3571..3575)
                     /label="CREB half"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(3508..3512)
                     /label="CREB half"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     enhancer        3221..3452
                     /label="core insulator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(join(7028..7662,1..181))
                     /label="Kan"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_binding    1962..2536
                     /label="EMCV IRES"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5603..5842
                     /label="SV40_pA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2537..2959
                     /label="Blasticidin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2980..3212
                     /label="BGH poly A terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    717..1955
                     /label="ADRB2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3738..5390
                     /label="luciferase"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     attB            570..613
                     /label="attB [BxB1]"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    633..641
                     /label="Kozak"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3675..3705
                     /label="minP"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
       61 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
      121 catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca
      181 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      241 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      301 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
      361 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
      421 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
      481 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
      541 agcagattgt actgagagtg cacaagcttc cggcttgtcg acgacggcgg tctccgtcgt
      601 caggatCATC CGGGGTAGTA ATAAGGGCGA CCGCCGCCAC CATGGACTAC AAAGACCATG
      661 ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGATAAA GGATCCGGCC
      721 AGCCTGGCAA TGGCAGCGCC TTTCTGCTGG CCCCTAATGG CTCTCACGCC CCCGATCACG
      781 ACGTGACCCA GGAACGGGAT GAAGTGTGGG TCGTGGGCAT GGGCATCGTG ATGAGCCTGA
      841 TCGTGCTGGC CATCGTGTTC GGCAACGTGC TCGTGATCAC CGCTATCGCC AAGTTCGAGC
      901 GGCTGCAGAC CGTGACCAAC TACTTCATCA CCTCCCTGGC CTGCGCCGAC CTCGTGATGG
      961 GACTGGCTGT GGTGCCTTTT GGAGCCGCCC ACATCCTGAT GAAGATGTGG ACCTTCGGCA
     1021 ACTTTTGGTG CGAGTTCTGG ACCAGCATCG ACGTGCTGTG CGTGACCGCC TCCATCGAGA
     1081 CACTGTGTGT GATCGCCGTG GACAGATACT TCGCCATCAC CAGCCCCTTC AAGTACCAGA
     1141 GCCTGCTGAC CAAGAACAAG GCCAGAGTGA TCATTCTGAT GGTGTGGATC GTGTCCGGCC
     1201 TGACCAGCTT CCTGCCCATC CAGATGCACT GGTACAGAGC CACCCACCAG GAAGCCATCA
     1261 ACTGCTACGC CAACGAAACC TGCTGCGACT TCTTCACAAA CCAGGCCTAC GCCATTGCCT
     1321 CCAGCATCGT GTCCTTCTAC GTGCCCCTCG TGATTATGGT GTTCGTGTAC AGCCGGGTGT
     1381 TCCAGGAAGC TAAGCGGCAG CTGCAGAAGA TCGACAAGAG CGAGGGCCGG TTCCACGTGC
     1441 AGAACCTGAG CCAGGTGGAA CAGGACGGCA GAACAGGCCA CGGCCTGCGG AGAAGCAGCA
     1501 AGTTCTGCCT GAAAGAGCAC AAGGCCCTGA AAACCCTGGG CATCATCATG GGCACCTTCA
     1561 CCCTGTGCTG GCTGCCCTTC TTCATCGTGA ACATCGTGCA CGTGATCCAG GACAACCTGA
     1621 TCCGGAAAGA GGTGTACATC CTGCTGAACT GGATCGGCTA CGTGAACAGC GGCTTCAACC
     1681 CCCTGATCTA CTGCAGAAGC CCCGACTTCC GGATCGCCTT CCAGGAACTG CTGTGCCTGA
     1741 GAAGAAGCAG CCTGAAGGCC TACGGCAACG GCTACAGCAG CAACGGCAAT ACCGGCGAGC
     1801 AGAGCGGCTA CCATGTGGAA CAGGAAAAAG AAAACAAGCT GCTGTGCGAG GACCTGCCCG
     1861 GCACCGAGGA TTTTGTGGGC CACCAGGGAA CCGTGCCCAG CGACAACATC GACAGCCAGG
     1921 GCAGAAACTG CAGCACCAAC GACAGCCTGC TGTGAGCTAG CCCCCTCTCC CTCCCCCCCC
     1981 CCTAACGTTA CTGGCCGAAG CCGCTTGGAA TAAGGCCGGT GTGCGTTTGT CTATATGTGA
     2041 TTTTCCACCA TATTGCCGTC TTTTGGCAAT GTGAGGGCCC GGAAACCTGG CCCTGTCTTC
     2101 TTGACGAGCA TTCCTAGGGG TCTTTCCCCT CTCGCCAAAG GAATGCAAGG TCTGTTGAAT
     2161 GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT TCTTGAAGAC AAACAACGTC TGTAGCGACC
     2221 CTTTGCAGGC AGCGGAACCC CCCACCTGGC GACAGGTGCC TCTGCGGCCA AAAGCCACGT
     2281 GTATAAGATA CACCTGCAAA GGCGGCACAA CCCCAGTGCC ACGTTGTGAG TTGGATAGTT
     2341 GTGGAAAGAG TCAAATGGCT CTCCTCAAGC GTATTCAACA AGGGGCTGAA GGATGCCCAG
     2401 AAGGTACCCC ATTGTATGGG ATCTGATCTG GGGCCTCGGT GCACATGCTT TACATGTGTT
     2461 TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG GGGACGTGGT TTTCCTTTGA
     2521 AAAACACGAT GATAATATGA AAACCTTCAA CATCTCTCAG CAGGATCTGG AGCTGGTGGA
     2581 GGTCGCCACT GAGAAGATCA CCATGCTCTA TGAGGACAAC AAGCACCATG TCGGGGCGGC
     2641 CATCAGGACC AAGACTGGGG AGATCATCTC TGCTGTCCAC ATTGAAGCCT ACATTGGCAG
     2701 GGTCACTGTC TGTGCTGAAG CCATTGCCAT TGGGTCTGCT GTGAGCAACG GGCAGAAGGA
     2761 CTTTGACACC ATTGTGGCTG TCAGGCACCC CTACTCTGAT GAGGTGGACA GATCCATCAG
     2821 GGTGGTCAGC CCCTGTGGCA TGTGTAGAGA GCTGATCTCT GACTATGCTC CTGACTGCTT
     2881 TGTGCTCATT GAGATGAATG GCAAGCTGGT CAAAACCACC ATTGAGGAAC TCATCCCCCT
     2941 CAAGTACACC AGGAACTAAT CTCGTTCGAA AATCATCGCg gcccgactgt gccttctagt
     3001 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3061 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3121 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3181 aggcatgctg gggatgcggt gggctctatg gCACTTTTAT GAGGGACAGC CCCCCCCCAA
     3241 AGCCCCCAGG GATGTAATTA CGTCCCTCCC CCGCTAGGGG GCAGCAGCGA GCCGCCCGGG
     3301 GCTCCGCTCC GGTCCGGCGC TCCCCCCGCA TCCCCGAGCC GGCAGCGTGC GGGGACAGCC
     3361 CGGGCACGGG GAAGGTGGCA CGGGATCGCT TTCCTCTGAA CGCTTCTCGC TGCTCTTTGA
     3421 GCCTGCAGAC ACCTGGGGGG ATACGGGGAA AAGGACGCGT gcaccagaca gtgacgtcag
     3481 ctgccagatc ccatggccgt catactgtga cgtctttcag acagcaccag acagtgacgt
     3541 cagctgccag atCCCatggc cgtcatactg tgacgtcttt cagacacccc attgacgtca
     3601 atgggagaac GGTACCTGAG CTCGCTAGCC TCGAGGATAT CAAGATCTGG CCTCGGCGGC
     3661 CAAGCTTAGA CACTAGAGGG TATATAATGG AAGCTCGACT TCCAGCTTGG CAATCCGGTA
     3721 CTGTTGGTAA AGCCACCATG GAAGATGCCA AAAACATTAA GAAGGGCCCA GCGCCATTCT
     3781 ACCCACTCGA AGACGGGACC GCCGGCGAGC AGCTGCACAA AGCCATGAAG CGCTACGCCC
     3841 TGGTGCCCGG CACCATCGCC TTTACCGACG CACATATCGA GGTGGACATT ACCTACGCCG
     3901 AGTACTTCGA GATGAGCGTT CGGCTGGCAG AAGCTATGAA GCGCTATGGG CTGAATACAA
     3961 ACCATCGGAT CGTGGTGTGC AGCGAGAATA GCTTGCAGTT CTTCATGCCC GTGTTGGGTG
     4021 CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG CTAACGACAT CTACAACGAG CGCGAGCTGC
     4081 TGAACAGCAT GGGCATCAGC CAGCCCACCG TCGTATTCGT GAGCAAGAAA GGGCTGCAAA
     4141 AGATCCTCAA CGTGCAAAAG AAGCTACCGA TCATACAAAA GATCATCATC ATGGATAGCA
     4201 AGACCGACTA CCAGGGCTTC CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCG
     4261 GCTTCAACGA GTACGACTTC GTGCCCGAGA GCTTCGACCG GGACAAAACC ATCGCCCTGA
     4321 TCATGAACAG TAGTGGCAGT ACCGGATTGC CCAAGGGCGT AGCCCTACCG CACCGCACCG
     4381 CTTGTGTCCG ATTCAGTCAT GCCCGCGACC CCATCTTCGG CAACCAGATC ATCCCCGACA
     4441 CCGCTATCCT CAGCGTGGTG CCATTTCACC ACGGCTTCGG CATGTTCACC ACGCTGGGCT
     4501 ACTTGATCTG CGGCTTTCGG GTCGTGCTCA TGTACCGCTT CGAGGAGGAG CTATTCTTGC
     4561 GCAGCTTGCA AGACTATAAG ATTCAATCTG CCCTGCTGGT GCCCACACTA TTTAGCTTCT
     4621 TCGCTAAGAG CACTCTCATC GACAAGTACG ACCTAAGCAA CTTGCACGAG ATCGCCAGCG
     4681 GCGGGGCGCC GCTCAGCAAG GAGGTAGGTG AGGCCGTGGC CAAACGCTTC CACCTACCAG
     4741 GCATCCGCCA GGGCTACGGC CTGACAGAAA CAACCAGCGC CATTCTGATC ACCCCCGAAG
     4801 GGGACGACAA GCCTGGCGCA GTAGGCAAGG TGGTGCCCTT CTTCGAGGCT AAGGTGGTGG
     4861 ACTTGGACAC CGGTAAGACA CTGGGTGTGA ACCAGCGCGG CGAGCTGTGC GTCCGTGGCC
     4921 CCATGATCAT GAGCGGCTAC GTTAACAACC CCGAGGCTAC AAACGCTCTC ATCGACAAGG
     4981 ACGGCTGGCT GCACAGCGGC GACATCGCCT ACTGGGACGA GGACGAGCAC TTCTTCATCG
     5041 TGGACCGGCT GAAGAGCCTG ATCAAATACA AGGGCTACCA GGTAGCCCCA GCCGAACTGG
     5101 AGAGCATCCT GCTGCAACAC CCCAACATCT TCGACGCCGG GGTCGCCGGC CTGCCCGACG
     5161 ACGATGCCGG CGAGCTGCCC GCCGCAGTCG TCGTGCTGGA ACACGGTAAA ACCATGACCG
     5221 AGAAGGAGAT CGTGGACTAT GTGGCCAGCC AGGTTACAAC CGCCAAGAAG CTGCGCGGTG
     5281 GTGTTGTGTT CGTGGACGAG GTGCCTAAAG GACTGACCGG CAAGTTGGAC GCCCGCAAGA
     5341 TCCGCGAGAT TCTCATTAAG GCCAAGAAGG GCGGCAAGAT CGCCGTGTAA TAATACCGGT
     5401 GCTTCACAAA CACAGCGACG CATCTGATTA AAGGGGGTAC CTTATGTTCA CAACTGGCGT
     5461 Gaatgtaact gtattcagcg atgacccaca caggcataga gtgtctgcta ttaataacta
     5521 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     5581 gatgtatagt gccttgacta gaATCTAGAT AACTgatcat aatcagccat accacatttg
     5641 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     5701 tgaatgCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
     5761 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
     5821 CCAAACTCAT CAATGTATCT TAggcgcgcc GTTAACgata tccatggtca tagctgtttc
     5881 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5941 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6001 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6061 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6121 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6181 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6241 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6301 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6361 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6421 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6481 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6541 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6601 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6661 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6721 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6781 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7021 ctgacagtta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     7081 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     7141 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     7201 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     7261 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     7321 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     7381 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     7441 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     7501 caggatattc ttctaatacc tggaatgctg ttttcccagg gatcgcagtg gtgagtaacc
     7561 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     7621 gccagtttag tctgaccatc tcatctgtaa catcattggc aa
//