LOCUS       Bxb1Donor_N51L_ADRB2_Lu 7659 bp ds-DNA     circular     10-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     attB            570..613
                     /label="attB [BxB1]"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     enhancer        3218..3449
                     /label="core insulator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3495..3499
                     /label="CREB half"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    633..641
                     /label="Kozak"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3469..3476
                     /label="CREB full"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2977..3209
                     /label="BGH poly A terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3672..3702
                     /label="minP"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(join(7025..7659,1..181))
                     /label="Kan"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3521..3607
                     /label="CRE segment"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3532..3539
                     /label="CREB full"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(3505..3509)
                     /label="CREB half"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2534..2956
                     /label="Blasticidin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3735..5387
                     /label="luciferase"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(3568..3572)
                     /label="CREB half"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3558..3562
                     /label="CREB half"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_binding    1959..2533
                     /label="EMCV IRES"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    717..1952
                     /label="ADRB2"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3590..3597
                     /label="CREB full"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    642..710
                     /label="3x FLAG Tag"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5600..5839
                     /label="SV40_pA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3458..3607
                     /label="CRE Enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
       61 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
      121 catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca
      181 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      241 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      301 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
      361 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
      421 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
      481 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
      541 agcagattgt actgagagtg cacaagcttc cggcttgtcg acgacggcgg tctccgtcgt
      601 caggatCATC CGGGGTAGTA ATAAGGGCGA CCGCCGCCAC CATGGACTAC AAAGACCATG
      661 ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGATAAA GGATCCGGCC
      721 AGCCTGGCAA TGGCAGCGCC TTTCTGCTGG CCCCTAATGG CTCTCACGCC CCCGATCACG
      781 ACGTGACCCA GGAACGGGAT GAAGTGTGGG TCGTGGGCAT GGGCATCGTG ATGAGCCTGA
      841 TCGTGCTGGC CATCGTGTTC GGCCTGGTGC TCGTGATCAC CGCTATCGCC AAGTTCGAGC
      901 GGCTGCAGAC CGTGACCAAC TACTTCATCA CCTCCCTGGC CTGCGCCGAC CTCGTGATGG
      961 GACTGGCTGT GGTGCCTTTT GGAGCCGCCC ACATCCTGAT GAAGATGTGG ACCTTCGGCA
     1021 ACTTTTGGTG CGAGTTCTGG ACCAGCATCG ACGTGCTGTG CGTGACCGCC TCCATCGAGA
     1081 CACTGTGTGT GATCGCCGTG GACAGATACT TCGCCATCAC CAGCCCCTTC AAGTACCAGA
     1141 GCCTGCTGAC CAAGAACAAG GCCAGAGTGA TCATTCTGAT GGTGTGGATC GTGTCCGGCC
     1201 TGACCAGCTT CCTGCCCATC CAGATGCACT GGTACAGAGC CACCCACCAG GAAGCCATCA
     1261 ACTGCTACGC CAACGAAACC TGCTGCGACT TCTTCACAAA CCAGGCCTAC GCCATTGCCT
     1321 CCAGCATCGT GTCCTTCTAC GTGCCCCTCG TGATTATGGT GTTCGTGTAC AGCCGGGTGT
     1381 TCCAGGAAGC TAAGCGGCAG CTGCAGAAGA TCGACAAGAG CGAGGGCCGG TTCCACGTGC
     1441 AGAACCTGAG CCAGGTGGAA CAGGACGGCA GAACAGGCCA CGGCCTGCGG AGAAGCAGCA
     1501 AGTTCTGCCT GAAAGAGCAC AAGGCCCTGA AAACCCTGGG CATCATCATG GGCACCTTCA
     1561 CCCTGTGCTG GCTGCCCTTC TTCATCGTGA ACATCGTGCA CGTGATCCAG GACAACCTGA
     1621 TCCGGAAAGT GTACATCCTG CTGAACTGGA TCGGCTACGT GAACAGCGGC TTCAACCCCC
     1681 TGATCTACTG CAGAAGCCCC GACTTCCGGA TCGCCTTCCA GGAACTGCTG TGCCTGAGAA
     1741 GAAGCAGCCT GAAGGCCTAC GGCAACGGCT ACAGCAGCAA CGGCAATACC GGCGAGCAGA
     1801 GCGGCTACCA TGTGGAACAG GAAAAAGAAA ACAAGCTGCT GTGCGAGGAC CTGCCCGGCA
     1861 CCGAGGATTT TGTGGGCCAC CAGGGAACCG TGCCCAGCGA CAACATCGAC AGCCAGGGCA
     1921 GAAACTGCAG CACCAACGAC AGCCTGCTGT GAGCTAGCCC CCTCTCCCTC CCCCCCCCCT
     1981 AACGTTACTG GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTGATTT
     2041 TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG
     2101 ACGAGCATTC CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC
     2161 GTGAAGGAAG CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT
     2221 TGCAGGCAGC GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA
     2281 TAAGATACAC CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG
     2341 GAAAGAGTCA AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG
     2401 GTACCCCATT GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG
     2461 TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT CCTTTGAAAA
     2521 ACACGATGAT AATATGAAAA CCTTCAACAT CTCTCAGCAG GATCTGGAGC TGGTGGAGGT
     2581 CGCCACTGAG AAGATCACCA TGCTCTATGA GGACAACAAG CACCATGTCG GGGCGGCCAT
     2641 CAGGACCAAG ACTGGGGAGA TCATCTCTGC TGTCCACATT GAAGCCTACA TTGGCAGGGT
     2701 CACTGTCTGT GCTGAAGCCA TTGCCATTGG GTCTGCTGTG AGCAACGGGC AGAAGGACTT
     2761 TGACACCATT GTGGCTGTCA GGCACCCCTA CTCTGATGAG GTGGACAGAT CCATCAGGGT
     2821 GGTCAGCCCC TGTGGCATGT GTAGAGAGCT GATCTCTGAC TATGCTCCTG ACTGCTTTGT
     2881 GCTCATTGAG ATGAATGGCA AGCTGGTCAA AACCACCATT GAGGAACTCA TCCCCCTCAA
     2941 GTACACCAGG AACTAATCTC GTTCGAAAAT CATCGCggcc cgactgtgcc ttctagttgc
     3001 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3061 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     3121 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     3181 catgctgggg atgcggtggg ctctatggCA CTTTTATGAG GGACAGCCCC CCCCCAAAGC
     3241 CCCCAGGGAT GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT
     3301 CCGCTCCGGT CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG
     3361 GCACGGGGAA GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC
     3421 TGCAGACACC TGGGGGGATA CGGGGAAAAG GACGCGTgca ccagacagtg acgtcagctg
     3481 ccagatccca tggccgtcat actgtgacgt ctttcagaca gcaccagaca gtgacgtcag
     3541 ctgccagatC CCatggccgt catactgtga cgtctttcag acaccccatt gacgtcaatg
     3601 ggagaacGGT ACCTGAGCTC GCTAGCCTCG AGGATATCAA GATCTGGCCT CGGCGGCCAA
     3661 GCTTAGACAC TAGAGGGTAT ATAATGGAAG CTCGACTTCC AGCTTGGCAA TCCGGTACTG
     3721 TTGGTAAAGC CACCATGGAA GATGCCAAAA ACATTAAGAA GGGCCCAGCG CCATTCTACC
     3781 CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG
     3841 TGCCCGGCAC CATCGCCTTT ACCGACGCAC ATATCGAGGT GGACATTACC TACGCCGAGT
     3901 ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAAGCG CTATGGGCTG AATACAAACC
     3961 ATCGGATCGT GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG TTGGGTGCCC
     4021 TGTTCATCGG TGTGGCTGTG GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA
     4081 ACAGCATGGG CATCAGCCAG CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCAAAAGA
     4141 TCCTCAACGT GCAAAAGAAG CTACCGATCA TACAAAAGAT CATCATCATG GATAGCAAGA
     4201 CCGACTACCA GGGCTTCCAA AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCGGCT
     4261 TCAACGAGTA CGACTTCGTG CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA
     4321 TGAACAGTAG TGGCAGTACC GGATTGCCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT
     4381 GTGTCCGATT CAGTCATGCC CGCGACCCCA TCTTCGGCAA CCAGATCATC CCCGACACCG
     4441 CTATCCTCAG CGTGGTGCCA TTTCACCACG GCTTCGGCAT GTTCACCACG CTGGGCTACT
     4501 TGATCTGCGG CTTTCGGGTC GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA
     4561 GCTTGCAAGA CTATAAGATT CAATCTGCCC TGCTGGTGCC CACACTATTT AGCTTCTTCG
     4621 CTAAGAGCAC TCTCATCGAC AAGTACGACC TAAGCAACTT GCACGAGATC GCCAGCGGCG
     4681 GGGCGCCGCT CAGCAAGGAG GTAGGTGAGG CCGTGGCCAA ACGCTTCCAC CTACCAGGCA
     4741 TCCGCCAGGG CTACGGCCTG ACAGAAACAA CCAGCGCCAT TCTGATCACC CCCGAAGGGG
     4801 ACGACAAGCC TGGCGCAGTA GGCAAGGTGG TGCCCTTCTT CGAGGCTAAG GTGGTGGACT
     4861 TGGACACCGG TAAGACACTG GGTGTGAACC AGCGCGGCGA GCTGTGCGTC CGTGGCCCCA
     4921 TGATCATGAG CGGCTACGTT AACAACCCCG AGGCTACAAA CGCTCTCATC GACAAGGACG
     4981 GCTGGCTGCA CAGCGGCGAC ATCGCCTACT GGGACGAGGA CGAGCACTTC TTCATCGTGG
     5041 ACCGGCTGAA GAGCCTGATC AAATACAAGG GCTACCAGGT AGCCCCAGCC GAACTGGAGA
     5101 GCATCCTGCT GCAACACCCC AACATCTTCG ACGCCGGGGT CGCCGGCCTG CCCGACGACG
     5161 ATGCCGGCGA GCTGCCCGCC GCAGTCGTCG TGCTGGAACA CGGTAAAACC ATGACCGAGA
     5221 AGGAGATCGT GGACTATGTG GCCAGCCAGG TTACAACCGC CAAGAAGCTG CGCGGTGGTG
     5281 TTGTGTTCGT GGACGAGGTG CCTAAAGGAC TGACCGGCAA GTTGGACGCC CGCAAGATCC
     5341 GCGAGATTCT CATTAAGGCC AAGAAGGGCG GCAAGATCGC CGTGTAATAA TACCGGTGCT
     5401 TCACAAACAC AGCGACGCAT CTGATTAAAG GGGGTACCTT ATGTTCACAA CTGGCGTGaa
     5461 tgtaactgta ttcagcgatg acccacacag gcatagagtg tctgctatta ataactatgc
     5521 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
     5581 gtatagtgcc ttgactagaA TCTAGATAAC Tgatcataat cagccatacc acatttgtag
     5641 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     5701 atgCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
     5761 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
     5821 AACTCATCAA TGTATCTTAg gcgcgccGTT AACgatatcc atggtcatag ctgtttcctg
     5881 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5941 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6001 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6061 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6121 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6181 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6241 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6301 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6361 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6421 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6481 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6601 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6661 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6721 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6781 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6841 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6901 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6961 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7021 acagttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     7081 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     7141 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     7201 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     7261 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag
     7321 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     7381 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     7441 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     7501 gatattcttc taatacctgg aatgctgttt tcccagggat cgcagtggtg agtaaccatg
     7561 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     7621 agtttagtct gaccatctca tctgtaacat cattggcaa
//