LOCUS Bxb1Donor_G102H_ADRB2_L 7659 bp ds-DNA circular 10-JUL-2019 DEFINITION . FEATURES Location/Qualifiers misc_feature 3735..5387 /label="luciferase" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature complement(3568..3572) /label="CREB half" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 717..1952 /label="ADRB2" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 2534..2956 /label="Blasticidin" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 3672..3702 /label="minP" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 enhancer 3218..3449 /label="core insulator" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 3532..3539 /label="CREB full" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 3458..3607 /label="CRE Enhancer" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 attB 570..613 /label="attB [BxB1]" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 3590..3597 /label="CREB full" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(join(7025..7659,1..181)) /label="Kan" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_binding 1959..2533 /label="EMCV IRES" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 5600..5839 /label="SV40_pA" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 3521..3607 /label="CRE segment" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(3505..3509) /label="CREB half" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 3558..3562 /label="CREB half" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 633..641 /label="Kozak" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 3469..3476 /label="CREB full" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 642..710 /label="3x FLAG Tag" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 2977..3209 /label="BGH poly A terminator" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 3495..3499 /label="CREB half" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 ORIGIN 1 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat 61 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag 121 catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca 181 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 241 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 301 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 361 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 421 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 481 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 541 agcagattgt actgagagtg cacaagcttc cggcttgtcg acgacggcgg tctccgtcgt 601 caggatCATC CGGGGTAGTA ATAAGGGCGA CCGCCGCCAC CATGGACTAC AAAGACCATG 661 ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGATAAA GGATCCGGCC 721 AGCCTGGCAA TGGCAGCGCC TTTCTGCTGG CCCCTAATGG CTCTCACGCC CCCGATCACG 781 ACGTGACCCA GGAACGGGAT GAAGTGTGGG TCGTGGGCAT GGGCATCGTG ATGAGCCTGA 841 TCGTGCTGGC CATCGTGTTC GGCAACGTGC TCGTGATCAC CGCTATCGCC AAGTTCGAGC 901 GGCTGCAGAC CGTGACCAAC TACTTCATCA CCTCCCTGGC CTGCGCCGAC CTCGTGATGG 961 GACTGGCTGT GGTGCCTTTT GGAGCCGCCC ACATCCTGAT GAAGATGTGG ACCTTCCACA 1021 ACTTTTGGTG CGAGTTCTGG ACCAGCATCG ACGTGCTGTG CGTGACCGCC TCCATCGAGA 1081 CACTGTGTGT GATCGCCGTG GACAGATACT TCGCCATCAC CAGCCCCTTC AAGTACCAGA 1141 GCCTGCTGAC CAAGAACAAG GCCAGAGTGA TCATTCTGAT GGTGTGGATC GTGTCCGGCC 1201 TGACCAGCTT CCTGCCCATC CAGATGCACT GGTACAGAGC CACCCACCAG GAAGCCATCA 1261 ACTGCTACGC CAACGAAACC TGCTGCGACT TCTTCACAAA CCAGGCCTAC GCCATTGCCT 1321 CCAGCATCGT GTCCTTCTAC GTGCCCCTCG TGATTATGGT GTTCGTGTAC AGCCGGGTGT 1381 TCCAGGAAGC TAAGCGGCAG CTGCAGAAGA TCGACAAGAG CGAGGGCCGG TTCCACGTGC 1441 AGAACCTGAG CCAGGTGGAA CAGGACGGCA GAACAGGCCA CGGCCTGCGG AGAAGCAGCA 1501 AGTTCTGCCT GAAAGAGCAC AAGGCCCTGA AAACCCTGGG CATCATCATG GGCACCTTCA 1561 CCCTGTGCTG GCTGCCCTTC TTCATCGTGA ACATCGTGCA CGTGATCCAG GACAACCTGA 1621 TCCGGAAAGT GTACATCCTG CTGAACTGGA TCGGCTACGT GAACAGCGGC TTCAACCCCC 1681 TGATCTACTG CAGAAGCCCC GACTTCCGGA TCGCCTTCCA GGAACTGCTG TGCCTGAGAA 1741 GAAGCAGCCT GAAGGCCTAC GGCAACGGCT ACAGCAGCAA CGGCAATACC GGCGAGCAGA 1801 GCGGCTACCA TGTGGAACAG GAAAAAGAAA ACAAGCTGCT GTGCGAGGAC CTGCCCGGCA 1861 CCGAGGATTT TGTGGGCCAC CAGGGAACCG TGCCCAGCGA CAACATCGAC AGCCAGGGCA 1921 GAAACTGCAG CACCAACGAC AGCCTGCTGT GAGCTAGCCC CCTCTCCCTC CCCCCCCCCT 1981 AACGTTACTG GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTGATTT 2041 TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG 2101 ACGAGCATTC CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC 2161 GTGAAGGAAG CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT 2221 TGCAGGCAGC GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA 2281 TAAGATACAC CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG 2341 GAAAGAGTCA AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG 2401 GTACCCCATT GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG 2461 TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT CCTTTGAAAA 2521 ACACGATGAT AATATGAAAA CCTTCAACAT CTCTCAGCAG GATCTGGAGC TGGTGGAGGT 2581 CGCCACTGAG AAGATCACCA TGCTCTATGA GGACAACAAG CACCATGTCG GGGCGGCCAT 2641 CAGGACCAAG ACTGGGGAGA TCATCTCTGC TGTCCACATT GAAGCCTACA TTGGCAGGGT 2701 CACTGTCTGT GCTGAAGCCA TTGCCATTGG GTCTGCTGTG AGCAACGGGC AGAAGGACTT 2761 TGACACCATT GTGGCTGTCA GGCACCCCTA CTCTGATGAG GTGGACAGAT CCATCAGGGT 2821 GGTCAGCCCC TGTGGCATGT GTAGAGAGCT GATCTCTGAC TATGCTCCTG ACTGCTTTGT 2881 GCTCATTGAG ATGAATGGCA AGCTGGTCAA AACCACCATT GAGGAACTCA TCCCCCTCAA 2941 GTACACCAGG AACTAATCTC GTTCGAAAAT CATCGCggcc cgactgtgcc ttctagttgc 3001 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 3061 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 3121 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 3181 catgctgggg atgcggtggg ctctatggCA CTTTTATGAG GGACAGCCCC CCCCCAAAGC 3241 CCCCAGGGAT GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT 3301 CCGCTCCGGT CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG 3361 GCACGGGGAA GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC 3421 TGCAGACACC TGGGGGGATA CGGGGAAAAG GACGCGTgca ccagacagtg acgtcagctg 3481 ccagatccca tggccgtcat actgtgacgt ctttcagaca gcaccagaca gtgacgtcag 3541 ctgccagatC CCatggccgt catactgtga cgtctttcag acaccccatt gacgtcaatg 3601 ggagaacGGT ACCTGAGCTC GCTAGCCTCG AGGATATCAA GATCTGGCCT CGGCGGCCAA 3661 GCTTAGACAC TAGAGGGTAT ATAATGGAAG CTCGACTTCC AGCTTGGCAA TCCGGTACTG 3721 TTGGTAAAGC CACCATGGAA GATGCCAAAA ACATTAAGAA GGGCCCAGCG CCATTCTACC 3781 CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG 3841 TGCCCGGCAC CATCGCCTTT ACCGACGCAC ATATCGAGGT GGACATTACC TACGCCGAGT 3901 ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAAGCG CTATGGGCTG AATACAAACC 3961 ATCGGATCGT GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG TTGGGTGCCC 4021 TGTTCATCGG TGTGGCTGTG GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA 4081 ACAGCATGGG CATCAGCCAG CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCAAAAGA 4141 TCCTCAACGT GCAAAAGAAG CTACCGATCA TACAAAAGAT CATCATCATG GATAGCAAGA 4201 CCGACTACCA GGGCTTCCAA AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCGGCT 4261 TCAACGAGTA CGACTTCGTG CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA 4321 TGAACAGTAG TGGCAGTACC GGATTGCCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT 4381 GTGTCCGATT CAGTCATGCC CGCGACCCCA TCTTCGGCAA CCAGATCATC CCCGACACCG 4441 CTATCCTCAG CGTGGTGCCA TTTCACCACG GCTTCGGCAT GTTCACCACG CTGGGCTACT 4501 TGATCTGCGG CTTTCGGGTC GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA 4561 GCTTGCAAGA CTATAAGATT CAATCTGCCC TGCTGGTGCC CACACTATTT AGCTTCTTCG 4621 CTAAGAGCAC TCTCATCGAC AAGTACGACC TAAGCAACTT GCACGAGATC GCCAGCGGCG 4681 GGGCGCCGCT CAGCAAGGAG GTAGGTGAGG CCGTGGCCAA ACGCTTCCAC CTACCAGGCA 4741 TCCGCCAGGG CTACGGCCTG ACAGAAACAA CCAGCGCCAT TCTGATCACC CCCGAAGGGG 4801 ACGACAAGCC TGGCGCAGTA GGCAAGGTGG TGCCCTTCTT CGAGGCTAAG GTGGTGGACT 4861 TGGACACCGG TAAGACACTG GGTGTGAACC AGCGCGGCGA GCTGTGCGTC CGTGGCCCCA 4921 TGATCATGAG CGGCTACGTT AACAACCCCG AGGCTACAAA CGCTCTCATC GACAAGGACG 4981 GCTGGCTGCA CAGCGGCGAC ATCGCCTACT GGGACGAGGA CGAGCACTTC TTCATCGTGG 5041 ACCGGCTGAA GAGCCTGATC AAATACAAGG GCTACCAGGT AGCCCCAGCC GAACTGGAGA 5101 GCATCCTGCT GCAACACCCC AACATCTTCG ACGCCGGGGT CGCCGGCCTG CCCGACGACG 5161 ATGCCGGCGA GCTGCCCGCC GCAGTCGTCG TGCTGGAACA CGGTAAAACC ATGACCGAGA 5221 AGGAGATCGT GGACTATGTG GCCAGCCAGG TTACAACCGC CAAGAAGCTG CGCGGTGGTG 5281 TTGTGTTCGT GGACGAGGTG CCTAAAGGAC TGACCGGCAA GTTGGACGCC CGCAAGATCC 5341 GCGAGATTCT CATTAAGGCC AAGAAGGGCG GCAAGATCGC CGTGTAATAA TACCGGTGCT 5401 TCACAAACAC AGCGACGCAT CTGATTAAAG GGGGTACCTT ATGTTCACAA CTGGCGTGaa 5461 tgtaactgta ttcagcgatg acccacacag gcatagagtg tctgctatta ataactatgc 5521 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat 5581 gtatagtgcc ttgactagaA TCTAGATAAC Tgatcataat cagccatacc acatttgtag 5641 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 5701 atgCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 5761 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 5821 AACTCATCAA TGTATCTTAg gcgcgccGTT AACgatatcc atggtcatag ctgtttcctg 5881 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 5941 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 6001 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6061 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6121 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6181 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6241 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6301 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6361 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6421 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6481 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 6601 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 6661 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 6721 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 6781 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 6841 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 6901 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 6961 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 7021 acagttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat 7081 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt 7141 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac 7201 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga 7261 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag 7321 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg 7381 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa 7441 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag 7501 gatattcttc taatacctgg aatgctgttt tcccagggat cgcagtggtg agtaaccatg 7561 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc 7621 agtttagtct gaccatctca tctgtaacat cattggcaa //