LOCUS       JPF0117_pLenti-PGK-tagB 9940 bp ds-DNA     circular     01-DEC-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             4803..5501
                     /label="tagBFP (yeast)"
     misc_recomb     complement(6770..6776)
                     /label="AttL2"
     misc_recomb     complement(6777..6790)
                     /label="attB2"
     misc_signal     5544..5606
                     /label="T2A"
     primer_bind     complement(9293..9312)
                     /label="T7 Forward (Elim)"
     misc_feature    9126..9203
                     /label="SV40_origin"
     misc_signal     4023..4038
                     /label="cPPT"
     CDS             3290..3523
                     /label="RRE_(HIV)"
     misc_signal     2736..2780
                     /label="HIV-1_psi_pack"
     CDS             5613..6671
                     /label="bPAC (human)"
     misc_feature    4754..4760
                     /label="Gateway recomb1"
     primer_bind     2139..2157
                     /label="M13 Reverse (Elim)"
     CDS             330..989
                     /label="AmpR"
     rep_origin      9483..9938
                     /label="M13 origin"
     CDS             7944..8543
                     /label="Puromycin"
     promoter        2448..2628
                     /label="HIV-1_5LTR"
     promoter        8734..8914
                     /label="HIV-1_5LTR"
     rep_origin      1087..1769
                     /label="ColE1 origin"
     misc_recomb     complement(6770..6776)
                     /label="attB2"
     primer_bind     complement(9322..9338)
                     /label="M13 Forward (-20) (Elim)"
     misc_recomb     4747..4753
                     /label="attB1"
     CDS             9410..9478
                     /label="LacZ alpha"
     primer_bind     complement(9342..9358)
                     /label="M13 Forward (-40) (Elim)"
     misc_recomb     4754..4767
                     /label="AttL1"
     misc_recomb     4754..4767
                     /label="attB1"
     promoter        2448..2628
                     /label="trunc_HIV-1_3LTR"
     promoter        4171..4693
                     /label="pgk1_promoter"
     misc_feature    complement(6777..6783)
                     /label="Gateway Recomb2"
     misc_signal     6824..7399
                     /label="WPRE"
     sig_peptide     6673..6699
                     /label="NES_(zhang)"
     sig_peptide     5502..5531
                     /label="NLS_(zhang)"
     misc_binding    2109..2131
                     /label="LacO"
     misc_feature    8989..9108
                     /label="SV40_PA_terminator"
     primer_bind     2174..2193
                     /label="T3 (Elim)"
     rep_origin      9500..9806
                     /label="F1 ori"
     misc_binding    6705..6734
                     /label="cMyc"
     primer_bind     complement(9295..9312)
                     /label="T7 Forward (short) (Elim)"
     misc_signal     8664..8679
                     /label="cPPT"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc accggTCGTC GACTAGTCCA GTGTGGTGGA ATTCTGCAGA
     4741 TATCAACAAG TTTGTACAAA AAAGCAGGCT CCACCATGGG AACCAATTCA GTCGACGTTA
     4801 ACATGAGCGA GCTGATTAAG GAGAACATGC ACATGAAGCT GTACATGGAG GGCACCGTGG
     4861 ACAACCATCA CTTCAAGTGC ACATCCGAGG GCGAAGGCAA GCCCTACGAG GGCACCCAGA
     4921 CCATGAGAAT CAAGGTGGTC GAGGGCGGCC CTCTCCCCTT CGCCTTCGAC ATCCTGGCTA
     4981 CTAGCTTCCT CTACGGCAGC AAGACCTTCA TCAACCACAC CCAGGGCATC CCCGACTTCT
     5041 TCAAGCAGTC CTTCCCTGAG GGCTTCACAT GGGAGAGAGT CACCACATAC GAAGACGGGG
     5101 GCGTGCTGAC CGCTACCCAG GACACCAGCC TCCAGGACGG CTGCCTCATC TACAACGTCA
     5161 AGATCAGAGG GGTGAACTTC ACATCCAACG GCCCTGTGAT GCAGAAGAAA ACACTCGGCT
     5221 GGGAGGCCTT CACCGAGACG CTGTACCCCG CTGACGGCGG CCTGGAAGGC AGAAACGACA
     5281 TGGCCCTGAA GCTCGTGGGC GGGAGCCATC TGATCGCAAA CATCAAGACC ACATATAGAT
     5341 CCAAGAAACC CGCTAAGAAC CTCAAGATGC CTGGCGTCTA CTATGTGGAC TACAGACTGG
     5401 AAAGAATCAA GGAGGCCAAC AACGAGACCT ACGTCGAGCA GCACGAGGTG GCAGTGGCCA
     5461 GATACTGCGA CCTCCCTAGC AAACTGGGGC ACAAGCTTAA TCCCAAGAAG AAGAGGAAGG
     5521 TGGAGGACGC CCGTACGAGT ACTGGAAGCG GAGAGGGCAG AGGAAGTCTG CTAACATGCG
     5581 GTGACGTCGA GGAGAATCCT GGACCTGGAT CCATGAAGCG GCTGGTGTAC ATCAGCAAGA
     5641 TCAGCGGCCA CCTGAGCCTG GAAGAGATCC AGCGGATCGG CAAGGTGTCC ATCAAGAACA
     5701 ACCAGCGGGA CAACATCACC GGCGTGCTGC TGTACCTGCA GGGCCTGTTC TTCCAGATCC
     5761 TGGAAGGCGA GAACGAGAAG GTGGACAAGC TGTACAAGAA AATCCTGGTG GACGACCGGC
     5821 ACACCAACAT CCTGTGCCTG AAAACCGAGT ACGACATCAC CGACCGGATG TTCCCCAACT
     5881 GGGCCATGAA AACCATCAAC CTGAACGAGA ACAGCGAGCT GATGATCCAG CCCATCAAGA
     5941 GCCTGCTGCA GACCATCACC CAGAGCCACC GGGTGCTGGA AAAGTACATG CCCGCCAGAG
     6001 TGATCTACCT GATCAACCAG GGCATCAACC CCCTGACCGT GGAGCCCCAG CTGGTCGAGA
     6061 AGATCATCTT CTTCAGCGAC ATCCTGGCCT TCAGCACCCT GACCGAGAAG CTGCCCGTGA
     6121 ACGAGGTGGT CATCCTGGTC AACCGGTACT TCAGCATCTG CACCCGGATC ATCAGCGCCT
     6181 ACGGCGGCGA AGTGACCAAG TTCATCGGCG ACTGCGTGAT GGCCAGCTTC ACCAAAGAGC
     6241 AGGGCGACGC CGCCATCCGG ACCAGCCTGG ACATCATCTC CGAGCTGAAG CAGCTGCGGC
     6301 ACCACGTGGA GGCCACCAAC CCCCTGCACC TGCTGTACAC CGGCATCGGC CTGAGCTACG
     6361 GCCACGTGAT CGAGGGCAAC ATGGGCAGCA GCCTGAAGAT GGACCACACC CTGCTGGGGG
     6421 ACGCCGTGAA CGTGGCCGCC AGGCTGGAAG CCCTGACAAG ACAGCTGCCC TACGCCCTGG
     6481 CCTTTACAGC CGGCGTGAAG AAGTGCTGCC AGGCCCAGTG GACCTTCATC AACCTGGGAG
     6541 CCCACCAGGT CAAGGGCAAG CAGGAAGCCA TCGAGGTCTA CACCGTGAAT GAGGCCCAGA
     6601 AGTACTACGA CACCCTGCAG ATCACCCAGC TGATCCGGCA GACCCTGGAA AACGACAAGC
     6661 CGCGGACGTA CGCTGCCCCC CCTGGAGAGG CTGACCCTGC GTACGAGCAA AAGCTGATCT
     6721 CGGAGGAGGA CCTGTAAGAA TTCGCGGCCG CACTCGAGAT ATCTAGACCC AGCTTTCTTG
     6781 TACAAAGTGG TTGATATCCA GCACAGTGGC GGCCGCTCGA caatcaacct ctggattaca
     6841 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6901 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6961 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7021 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7081 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7141 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7201 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     7261 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7321 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7381 gtcggatctc cctttgggcc gcctccccgc ctgGAATTCT ACCGGGTAGG GGAGGCGCTT
     7441 TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA
     7501 AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC GGCTCCGTTC
     7561 TTTGGTGGCC CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC
     7621 CCGCAGCTCG CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG
     7681 CAGATGGACA GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG
     7741 CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG
     7801 CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG CCCGGCATTC
     7861 TGCACGCTTC AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC TCCGGGCCTT
     7921 TCGACCTGCA GCCCAAGCTT ACCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC
     7981 GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA
     8041 CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT
     8101 TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG
     8161 TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC
     8221 CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC
     8281 TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG
     8341 ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC
     8401 GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC
     8461 GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA
     8521 TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC GCAGCGCCCG ACCGAAAGGA
     8581 GCGCACGACC CCATGCATCT CGAGGGCCCG GTACctttaa gaccaatgac ttacaaggca
     8641 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     8701 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     8761 gcctgggagc tctctggctg cctagggaac ccactgctta agcctcaata aagcttgcct
     8821 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8881 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     8941 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     9001 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9061 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     9121 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9181 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     9241 gaggaggctt ttttggaggc ctaggctttt gcgtcgagac gtacccaatt cgccctatag
     9301 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     9361 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     9421 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     9481 cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     9541 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     9601 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     9661 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     9721 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     9781 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     9841 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     9901 taacgcgaat tttaacaaaa tattaacgtt tacaatttcc
//