LOCUS       Exported                4269 bp ds-DNA     circular SYN 12-JUL-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Overexpression vector pWALIUM10-moe"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="/dnas_title=pCRISPaint-3xP3-GFP"
                     /note="color: #ffffff"
                     /db_xref="taxon:747297"
     misc_feature    549..568
                     /label=T2A-Gal4_round 1 primer
                     /note="T2A-Gal4_round 1 primer"
                     /note="color: #a6acb3"
     misc_feature    583..602
                     /label=T2A-Gal4_3'_F Round 2 primer
                     /note="T2A-Gal4_3'_F Round 2 primer"
                     /note="color: #a6acb3"
     misc_feature    603..618
                     /label=M13F sequencing primer
                     /note="M13F sequencing primer"
                     /note="color: #a6acb3"
     misc_feature    682..706
                     /label=multi target site
                     /note="multi target site"
                     /note="color: #a6acb3"
     misc_feature    682..704
                     /label=frame +2
                     /note="frame +2"
                     /note="color: #a6acb3"
     misc_feature    683..705
                     /label=frame +1
                     /note="frame +1"
                     /note="color: #a6acb3"
     misc_feature    684..706
                     /label=frame +0
                     /note="frame +0"
                     /note="color: #a6acb3"
     misc_feature    699..721
                     /label=frame2 aftercut
                     /note="frame2 aftercut"
                     /note="color: #a6acb3"
     misc_feature    700..721
                     /label=frame1 after cut
                     /note="frame1 after cut"
                     /note="color: #a6acb3"
     misc_feature    701..721
                     /label=frame0 after cut
                     /note="frame0 after cut"
                     /note="color: #a6acb3"
     misc_feature    776..2007
                     /note="color: #a6acb3"
     misc_feature    776..809
                     /label=loxp
                     /note="loxp"
                     /note="color: #a6acb3"
     misc_feature    816..1040
                     /label=3xP3-DsRed
                     /note="3xP3-DsRed"
                     /note="color: #a6acb3"
     misc_feature    complement(873..892)
                     /label=seqR
                     /note="seqR"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    1041..1757
                     /label=GFP
                     /note="GFP"
                     /note="color: #a6acb3"
     misc_feature    1041..1757
                     /label=P3GFP
                     /note="P3GFP"
                     /note="color: #a6acb3"
     misc_feature    1762..2002
                     /label=SV40
                     /note="SV40"
                     /note="color: #a6acb3"
     misc_feature    complement(2008..2070)
                     /label=reverse primer for insert
                     /note="reverse primer for insert"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    2008..2031
                     /label=random dna
                     /note="random dna"
                     /note="color: #a6acb3"
     misc_feature    2032..2065
                     /label=loxp
                     /note="loxp"
                     /note="color: #a6acb3"
     misc_feature    2032..2065
                     /note="color: #a6acb3"
     misc_feature    2067..2086
                     /note="forward to amplify backbone with same ends as 
                     AscI/SacI for gibson"
                     /note="color: #a6acb3"
     misc_feature    2123..2139
                     /label=M13R
                     /note="M13R"
                     /note="color: #a6acb3"
     misc_feature    2405..2424
                     /label=backbone geno foward
                     /note="backbone geno foward"
                     /note="color: #a6acb3"
     misc_feature    complement(2896..2915)
                     /label=backbone geno reverse
                     /note="backbone geno reverse"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    2896..2915
                     /label=backbone geno forward
                     /note="backbone geno forward"
                     /note="color: #a6acb3"
     misc_feature    3287..3946
                     /label=AmpR
                     /note="AmpR"
                     /note="color: #a6acb3"
     misc_feature    complement(3371..3390)
                     /label=backbone geno reverse
                     /note="backbone geno reverse"
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacaag
      661 cttcacgacc tgaggcgcgc cgggccagta cccaaaaagc ggggggtccg gtggaagcgg
      721 aggtagcgcg gccgcacgct agctgctcga gacggtacct agaattcaca gatctataac
      781 ttcgtataat gtatgctata cgaagttatc gtacgggatc taattcaatt agagactaat
      841 tcaattagag ctaattcaat taggatccaa gcttatcgat ttcgaaccct cgaccgccgg
      901 agtataaata gaggcgcttc gtctacggag cgacaattca attcaaacaa gcaaagtgaa
      961 cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc taaacaatcg
     1021 gctcgaagcc ggtcgccacc atggtgtcca agggcgagga gctgttcacc ggcgtggtgc
     1081 caattctggt ggagctggat ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
     1141 gcgagggcga cgccacctat ggaaagctga ccctgaagtt catctgcacc accggcaagc
     1201 tgcccgtgcc atggccaacc ctcgtgacca ccctgaccta tggcgtgcag tgcttcagcc
     1261 gctaccccga tcacatgaag cagcacgatt tcttcaagag cgccatgccc gagggctacg
     1321 tgcaggagcg caccatcttt ttcaaggatg acggcaacta caagacccgc gccgaagtga
     1381 agttcgaggg cgataccctc gtgaaccgca tcgagctgaa gggcatcgat ttcaaggagg
     1441 atggaaacat cctgggccac aagctggagt acaactacaa cagccacaac gtgtacatca
     1501 tggccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     1561 atggcggcgt gcagctggcc gatcactacc agcagaacac cccaatcggc gacggcccag
     1621 tgctgctgcc cgataaccat tacctgagca cccagagcgc cctgagcaag gatcccaacg
     1681 agaagcgcga ccacatggtg ctgctggagt ttgtgaccgc cgccggcatt accctgggca
     1741 tggatgagct gtacaagtag gggccgcgac tctagatcat aatcagccat accacatttg
     1801 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     1861 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     1921 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1981 ccaaactcat caatgtatct taaccgtctg taagcagcga aggcccatac gataacttcg
     2041 tataatgtat gctatacgaa gttatgagct ccagcttttg ttccctttag tgagggttaa
     2101 tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2161 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2221 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2281 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2341 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2401 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2461 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2521 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2581 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2641 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2701 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2761 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2821 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2881 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2941 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     3001 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3061 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3121 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3181 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3241 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3301 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3361 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3421 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3481 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3541 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3601 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3661 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3721 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3781 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3841 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3901 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3961 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4021 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4081 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4141 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4201 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4261 gaaaagtgc
//
