LOCUS       pAAV_CMV_COLA3_R        6948 bp ds-DNA     circular     12-JUL-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    5148..6008
                     /locus_tag="AMPr"
                     /ApEinfo_label="AMPr"
                     /ApEinfo_fwdcolor="#f0fd02"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1616..1620
                     /locus_tag="BbsImut"
                     /ApEinfo_label="BbsImut"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    2737..3312
                     /locus_tag="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#f9ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6163..6782
                     /locus_tag="pBR322 ori"
                     /ApEinfo_label="pBR322 ori"
                     /ApEinfo_fwdcolor="#defd02"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3266..3497
                     /locus_tag="bGH polyA"
                     /ApEinfo_label="bGH polyA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1187..1894
                     /locus_tag="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    568..1144
                     /locus_tag="CMV promoter"
                     /ApEinfo_label="CMV promoter"
                     /ApEinfo_fwdcolor="#473efd"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(552..559)
                     /note="beta-gal"
                     /locus_tag="beta-gal"
                     /ApEinfo_label="beta-gal"
                     /ApEinfo_fwdcolor="#5a0077"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    443..451
                     /locus_tag="CMV promoter(1)"
                     /ApEinfo_label="CMV promoter"
                     /ApEinfo_fwdcolor="#473efd"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(560..563)
                     /note="beta-gal"
                     /locus_tag="beta-gal(1)"
                     /ApEinfo_label="beta-gal"
                     /ApEinfo_fwdcolor="#5a0077"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    5078..5106
                     /locus_tag="AMPr promoter"
                     /ApEinfo_label="AMPr promoter"
                     /ApEinfo_fwdcolor="#feac01"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3720..3725
                     /locus_tag="WPRE(1)"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#f9ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     promoter        3726..3974
                     /dnas_title="Human U6 Promoter"
                     /vntifkey="29"
                     /locus_tag="Human U6 Promoter"
                     /ApEinfo_label="Human U6 Promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1939..1945
                     /locus_tag="Esp3I"
                     /ApEinfo_label="Esp3I"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    4217..4389
                     /locus_tag="ITR"
                     /ApEinfo_label="ITR"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1908..1911
                     /locus_tag="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    243..415
                     /locus_tag="ITR(1)"
                     /ApEinfo_label="ITR"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1935..1938
                     /locus_tag="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    3996..4071
                     /note="chimeric guide RNA scaffold"
                     /locus_tag="Human U6 Promoter(1)"
                     /ApEinfo_label="Human U6 Promoter"
                     /ApEinfo_fwdcolor="#fffd0d"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4072..4077
                     /note="U6 terminator"
                     /locus_tag="Human U6 Promoter(2)"
                     /ApEinfo_label="Human U6 Promoter"
                     /ApEinfo_fwdcolor="#fffd0d"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3726..3745
                     /locus_tag="primer for"
                     /ApEinfo_label="primer for"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    4148..4167
                     /locus_tag="primer rev"
                     /ApEinfo_label="primer rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    452..551
                     /locus_tag="DONOR A3"
                     /ApEinfo_label="DONOR A3"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1996..2714
                     /locus_tag="eGFP"
                     /ApEinfo_label="eGFP"
                     /ApEinfo_fwdcolor="#5aff67"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    3659..3709
                     /locus_tag="SV40 polyA signal"
                     /ApEinfo_label="SV40 polyA signal"
                     /ApEinfo_fwdcolor="#eab001"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    1901..1907
                     /locus_tag="Esp3I(1)"
                     /ApEinfo_label="Esp3I"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    1912..1934
                     /locus_tag="sgRNA target"
                     /ApEinfo_label="sgRNA target"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc
      301 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
      361 catcactagg ggttccttgt agttaatgat taacccgcca tgctacttat ctacgtagcc
      421 atgctctagg aagatcggaa ttcgccctta aAGACCCAGG AATTCCAGGC TTGGATAGAT
      481 CAGGATTTCC TGGAGAAACT GgATCACCAG GAATTCCAGG TCATCAAGGT GAAATGGGAC
      541 CACTGGGTCA ATCGACGGTA TCGaattcgc ccttaagcta gctagttatt aatagtaatc
      601 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      661 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      721 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      781 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      841 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      901 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      961 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     1021 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     1081 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     1141 aagcagagct ggtttagtga accgtcagat ccCTAGCGGA TTCACCATGG TGAGCAAGGG
     1201 CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG
     1261 CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG
     1321 CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT
     1381 CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC
     1441 CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA
     1501 GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA
     1561 CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAaAAGAC
     1621 CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA
     1681 GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCTGAGG TCAAGACCAC
     1741 CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA
     1801 CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG
     1861 CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGaagctt cgtctcTGTC GCCTGGAGAA
     1921 ACTGAATCAC CTGGTCTGcg agacgGAATT CTGCAaTCGA CGGTACCaCG GGCCCGGGAT
     1981 CCACCGGTCG CCACCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
     2041 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG
     2101 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
     2161 GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC
     2221 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG
     2281 GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC
     2341 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC
     2401 AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC
     2461 GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC
     2521 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
     2581 CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG
     2641 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC
     2701 GAGCTGTACA AGTAAAGCGG CCGCgcttgg atccaatcaa cctctggatt acaaaatttg
     2761 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2821 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2881 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2941 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3001 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3061 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3121 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     3181 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3241 cctgctgccg gctctgcggc ctcttccgcg tcttcgagat ctgcctcgac tgtgccttct
     3301 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     3361 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     3421 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     3481 agcaggcatg ctggggactc gagttaGCGG CCGCGACTCT AGATCATAAT CAGCCATACC
     3541 ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA
     3601 CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA
     3661 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGc
     3721 cgcgggaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
     3781 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
     3841 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
     3901 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttGTGGA
     3961 AAGGACGAAA CACCgCCTGG AGAAACTGAA TCACCgtttt agagctaGAA Atagcaagtt
     4021 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cTTTTTTgtt
     4081 ttagagctag aaatagcaag ttaaaataag gctagtccgt TTTTagcgcg tgcgccaatt
     4141 ctgcagacaa atggctctag aggtaccctc gagttaaggg cgaattcccg attaggatct
     4201 tcctagagca tggctacgta gataagtagc atggcgggtt aatcattaac tacaaggaac
     4261 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     4321 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     4381 gcagccttaa ttaacctaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4441 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4501 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     4561 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4621 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4681 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4741 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4801 catcgccccg atagacggtt tttcgccctt tgacgctgga gttcacgttc ctcaatagtg
     4861 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4921 aagggatttt tccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4981 acgcgaattt taacaaaata ttaacgttta taatttcagg tggcatcttt cggggaaatg
     5041 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5101 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5161 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5221 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5281 tcgaactgga tctcaatagt ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5341 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5401 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5461 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5521 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5581 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5641 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagtaatgg
     5701 taacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5761 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5821 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5881 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5941 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6001 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6061 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6121 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6181 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6241 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6301 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     6361 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6421 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6481 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6541 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6601 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6661 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6721 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6781 cggccttttt acggttcctg gccttttgct gcggttttgc tcacatgttc tttcctgcgt
     6841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag 
//
