LOCUS       Exported                4939 bp ds-DNA     circular SYN 03-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..154
                     /label=Upstream polyA / transcriptional pause
                     /note="Upstream polyA / transcriptional pause"
     misc_feature    162..664
                     /label=MCS
                     /note="MCS"
     misc_feature    complement(212..487)
                     /label=lacZ'
     promoter        complement(488..609)
                     /label=lacZ promoter
     misc_feature    682..937
                     /label=HSV-tk basal promoter
                     /note="HSV-tk basal promoter"
     misc_feature    988..1120
                     /label=chimeric intron
                     /note="chimeric intron"
     misc_feature    1180..1873
                     /label=Cerulean
                     /note="Cerulean"
     misc_feature    1923..2015
                     /label=Nanotag_80
     misc_feature    2054..2275
                     /label=SV40 late polyA region
                     /note="SV40 late polyA region"
     misc_feature    complement(3365..4222)
                     /label=Beta-lactamase AmpR gene
                     /note="Beta-lactamase AmpR gene"
     misc_feature    4355..4809
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
       61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      121 cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcgagttcc
      181 atgggagacg ggcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
      241 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      301 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      361 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc
      421 tcgaggtcct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
      481 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      541 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      601 gcgttgcgct cactgcccgc tttccaccgg tcgtctcagg accgctagcc cgggctcgag
      661 atctgcgatc taagtaagct tgctgcttca tccccgtggc ccgttgctcg cgtttgctgg
      721 cggtgtcccc ggaagaaata tatttgcatg tctttagttc tatgatgaca caaaccccgc
      781 ccagcgtctt gtcattggcg aattcgaaca cgcagatgca gtcggggcgg cgcggtccca
      841 ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg
      901 acccgcttaa cagcgtcaac agcgtgccgc agatcccaag cttgcggccg cttaagctgc
      961 agaagttggt cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga
     1021 gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct
     1081 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtccactc ccaggttcaa
     1141 tacagctctt aagcggccgc aagctagctt gccgccacca tggtgagcaa gggcgaggag
     1201 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1261 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1321 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
     1381 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1441 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1501 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1561 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc
     1621 agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     1681 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1741 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     1801 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1861 gccgggatca ctctcggcat ggacgagctg tacaagtgag cctctagact ccctcagcgg
     1921 cgcgtgagca ttacggagcc gactacactc aggcaggagc cgcttaagga gctgctcctc
     1981 taacgaacgg caagattggc agcgtagatg cgcctctcac gtgtctagag tcggggcggc
     2041 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     2101 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     2161 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     2221 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     2281 gataaggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     2341 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     2401 aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2461 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2521 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2581 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2641 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2701 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2761 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     2821 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2881 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2941 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3001 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3061 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3121 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3181 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3241 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3301 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3361 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3421 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3481 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3541 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3601 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3661 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3721 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3781 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3841 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3901 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3961 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4021 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4081 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4141 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4201 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4261 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4321 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg
     4381 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4441 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4501 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4561 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4621 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4681 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4741 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     4801 ttacaatttg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4861 cctcttcgct attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg
     4921 aggtacttgg agcggccgc
//
