LOCUS       Exported                5323 bp ds-DNA     circular SYN 05-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Weatherall Institute
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..154
                     /label=Upstream polyA / transcriptional pause
                     /note="Upstream polyA / transcriptional pause"
     misc_feature    162..179
                     /label=MCS
                     /note="MCS"
     misc_feature    1018..1043
                     /label=MCS
                     /note="MCS"
     misc_feature    1061..1316
                     /label=HSV-tk basal promoter
                     /note="HSV-tk basal promoter"
     misc_feature    1367..1499
                     /label=chimeric intron
                     /note="chimeric intron"
     misc_feature    1559..2278
                     /label=Citrine
                     /note="Citrine"
     misc_feature    2306..2395
                     /label=Nanotag_45
     misc_feature    2438..2659
                     /label=SV40 late polyA region
                     /note="SV40 late polyA region"
     misc_feature    complement(3749..4606)
                     /label=Beta-lactamase AmpR gene
                     /note="Beta-lactamase AmpR gene"
     misc_feature    4739..5193
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
       61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      121 cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcgagttgc
      181 caggtcctgg tctttaagct ctggacttcc tttttacatt taccactgta tagttcataa
      241 ttgtaatgag aatatttttc ccctcttgat gtaacatctc agggatcagc aaatctaggt
      301 ggtctttcca cctttaaaag aactgaaaaa ttttaatgga aattggagaa tagatgaatg
      361 agctaacagc agggttcaat tttaatgaca gcaacaagga attctgtaat taagcacatt
      421 aaggcagaac accctctttg aatttcagaa catctctagt tcatgctcta acatctgttt
      481 ttcttagact gctttaatct gcatcctccc ttcaggcatc ttacattaag ggtttgacag
      541 actttcctag ctgacagtag ctggcatcct tttaattgac aagggagacc actgttgaaa
      601 gagattggga agagcattct cttttccctc aaggtctaaa tgctctgctt ttgcagaact
      661 cctgcaggac ctgctagcta cagactcaac ctgaaaaggc agtttttgca gaatccaatg
      721 aagagctcaa ataagcttca catttgttcc actctctttt attttggatt tttgtcagat
      781 atcccaagag aaaaagtttt gtgttttatt ctccacatat gtgttatttt agcagaaagt
      841 gggataatct ccattaaaaa taataaaaat actgatgcat ctgcttacat ctcattaaaa
      901 tcagatacaa attaggctat ctaaaattct tatatatttg gttatgagcc tgaaacaacc
      961 ttttttttgt ttgtttgttt ttgtttttgt ttttgttttc ctgcattgtg gtcactctgt
     1021 tggctagccc gggctcgaga tctgcgatct aagtaagctt gctgcttcat ccccgtggcc
     1081 cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct
     1141 atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag
     1201 tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac
     1261 accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca gatcccaagc
     1321 ttgcggccgc ttaagctgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt
     1381 tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt
     1441 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg
     1501 tgtccactcc caggttcaat acagctctta agcggccgca agctagcttg ccgccaccat
     1561 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1621 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1681 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1741 cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca
     1801 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1861 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1921 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1981 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2041 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2101 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2161 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2221 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtgagc
     2281 ctctagactc cctcagcggc gcgcctggtg ttcctcgaga tcggagtcct aaatcgactg
     2341 ccttagcggt aggacagtag atcagtcagc gacgtgacga accctcgcag gatcaagatc
     2401 tcacgtgtct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
     2461 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2521 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2581 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2641 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt
     2701 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     2761 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc
     2821 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2881 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2941 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3001 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3061 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3121 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3181 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3241 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3301 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3361 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3421 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3481 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3541 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3601 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3661 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3721 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3781 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3841 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3901 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3961 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4021 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4741 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4801 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4861 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4921 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4981 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5041 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5101 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5161 cgaattttaa caaaatatta acgcttacaa tttgccattc gccattcagg ctgcgcaact
     5221 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat
     5281 aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgc
//
