LOCUS       Exported                5256 bp ds-DNA     circular SYN 06-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Weatherall Institute
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..154
                     /label=Upstream polyA / transcriptional pause
                     /note="Upstream polyA / transcriptional pause"
     misc_feature    162..179
                     /label=MCS
                     /note="MCS"
     misc_feature    951..976
                     /label=MCS
                     /note="MCS"
     misc_feature    994..1249
                     /label=HSV-tk basal promoter
                     /note="HSV-tk basal promoter"
     misc_feature    1300..1432
                     /label=chimeric intron
                     /note="chimeric intron"
     misc_feature    1492..2185
                     /label=Cerulean
                     /note="Cerulean"
     misc_feature    2235..2332
                     /label=Nanotag_89
     misc_feature    2371..2592
                     /label=SV40 late polyA region
                     /note="SV40 late polyA region"
     misc_feature    complement(3682..4539)
                     /label=Beta-lactamase AmpR gene
                     /note="Beta-lactamase AmpR gene"
     misc_feature    4672..5126
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
       61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      121 cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcgagttcc
      181 atggcccact agaccatgtt cccccagtgc cacatctatc tttttcttga tcacctccaa
      241 agatggtgac tgcaattata ctagtttctg cctttggata tgagtgctga ttttagttac
      301 aactagccag gtatatgtaa ttaagctcag attggactgc agtgctacct acagtaagac
      361 atgtgggagg cagtgggagc aggaatgctg ggagtactga gactttgcaa aacagagctg
      421 aaaattgaat cccacttgtg cctactccag aatctatctg gtattgtaac atatccttta
      481 agactgctaa aggattgatc ccaaactggg aggtgtcttt ccatctacct ctgtggtctt
      541 ttttacttgg aaatcttttg ttatcttgca gtaaatgctc tggtataagc tgaagttcca
      601 accctccttt tcttgcctct ggtgatccta tagagactgc tcaaattacc caagaggttt
      661 gagatagcgt attgttttca tggacttaac tctgtgtaat tatgcatgag taatcctcgt
      721 ttatttctgt tatcctttaa ttgagctata agatttttta agcttaaaaa ataccacagg
      781 ctttctgagg gctctaaatt aaaatgcaac aaaacaaaaa agtgcactcc ttagaccaag
      841 aatctgccat attttattgt aaaagaagtg gcttttgtgt tgaatccttc agaacaacag
      901 tgtggagtct ttttctttta ttttcaattg tcctgaagtg ctgtgtctca ggaccgctag
      961 cccgggctcg agatctgcga tctaagtaag cttgctgctt catccccgtg gcccgttgct
     1021 cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga
     1081 cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc
     1141 ggcgcggtcc caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc
     1201 gaccctgcag cgacccgctt aacagcgtca acagcgtgcc gcagatccca agcttgcggc
     1261 cgcttaagct gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga
     1321 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1381 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac
     1441 tcccaggttc aatacagctc ttaagcggcc gcaagctagc ttgccgccac catggtgagc
     1501 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1561 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1621 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1681 accctgacct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     1741 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1801 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1861 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1921 tacaacgcca tcagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     1981 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2041 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2101 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2161 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtg agcctctaga
     2221 ctccctcagc ggcgtcgcta agagaactcc ggtacgacgc gatgcgacta cagcagggac
     2281 cttcaagcgc tcttctcggg agaatcgcta gtcgcgcttg gataaccttc cgctcacgtg
     2341 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     2401 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     2461 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     2521 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     2581 acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca
     2641 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     2701 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
     2761 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2821 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     3121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3361 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3541 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3661 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3721 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3781 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3841 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3901 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3961 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4021 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4081 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4141 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4201 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4261 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4321 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4381 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4441 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4501 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4561 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4621 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
     4681 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4741 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4801 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4861 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4921 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4981 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5041 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5101 taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga
     5161 agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag
     5221 taatattaag gtacgggagg tacttggagc ggccgc
//
