LOCUS       pSL0376_(His-MBP-StrepI 7193 bp ds-DNA     circular     08-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2878..2900
                     /label="RBS"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4141..4164
                     /label="StrepII"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2959..4059
                     /label="MBP"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2920..2949
                     /label="His10"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4114..4134
                     /label="TEV site"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    5504..5551
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    6163..7023
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    6071..6162
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4180..5364
                     /label="VchTniQ"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1..589
                     /label="ColEI ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2771..2789
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
       61 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
      121 CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
      181 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
      241 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
      301 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
      361 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
      421 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
      481 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
      541 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
      601 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
      661 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
      721 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT
      781 GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC
      841 AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG
      901 ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT
      961 GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC
     1021 AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT
     1081 GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT
     1141 CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT
     1201 GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA
     1261 TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG
     1321 AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC
     1381 AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC
     1441 ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC
     1501 TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC
     1561 AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC
     1621 AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC
     1681 AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG
     1741 ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC
     1801 CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT
     1861 GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC
     1921 TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT
     1981 CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC
     2041 CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC
     2101 GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG
     2161 CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA
     2221 ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC
     2281 CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC
     2341 CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC
     2401 GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA
     2461 GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG
     2521 GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA
     2581 CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC
     2641 GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC
     2701 ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT
     2761 CCCGCGAAAT TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGTGCCGGC
     2821 TCCGGAGAGC TCTTTAATTA AGCGGCCGCC CTGCAGGACT CGAGTTCTAG AAATAATTTT
     2881 GTTTAACTTT AAGAAGGAGA TATACATATG AAATCTTCTc accatcacca tCACCATCAC
     2941 CATCACCATG GTTCTTCTAT GAAAATCGAA GAAGGTAAAC TGGTAATCTG GATTAACGGC
     3001 GATAAAGGCT ATAACGGTCT CGCTGAAGTC GGTAAGAAAT TCGAGAAAGA TACCGGAATT
     3061 AAAGTCACCG TTGAGCATCC GGATAAACTG GAAGAGAAAT TCCCACAGGT TGCGGCAACT
     3121 GGCGATGGCC CTGACATTAT CTTCTGGGCA CACGACCGCT TTGGTGGCTA CGCTCAATCT
     3181 GGCCTGTTGG CTGAAATCAC CCCGGACAAA GCGTTCCAGG ACAAGCTGTA TCCGTTTACC
     3241 TGGGATGCCG TACGTTACAA CGGCAAGCTG ATTGCTTACC CGATCGCTGT TGAAGCGTTA
     3301 TCGCTGATTT ATAACAAAGA TCTGCTGCCG AACCCGCCAA AAACCTGGGA AGAGATCCCG
     3361 GCGCTGGATA AAGAACTGAA AGCGAAAGGT AAGAGCGCGC TGATGTTCAA CCTGCAAGAA
     3421 CCGTACTTCA CCTGGCCGCT GATTGCTGCT GACGGGGGTT ATGCGTTCAA GTATGAAAAC
     3481 GGCAAGTACG ACATTAAAGA CGTGGGCGTG GATAACGCTG GCGCGAAAGC GGGTCTGACC
     3541 TTCCTGGTTG ACCTGATTAA AAACAAACAC ATGAATGCAG ACACCGATTA CTCCATCGCA
     3601 GAAGCTGCCT TTAATAAAGG CGAAACAGCG ATGACCATCA ACGGCCCGTG GGCATGGTCC
     3661 AACATCGACA CCAGCAAAGT GAATTATGGT GTAACGGTAC TGCCGACCTT CAAGGGTCAA
     3721 CCATCCAAAC CGTTCGTTGG CGTGCTGAGC GCAGGTATTA ACGCCGCCAG TCCGAACAAA
     3781 GAGCTGGCAA AAGAGTTCCT CGAAAACTAT CTGCTGACTG ATGAAGGTCT GGAAGCGGTT
     3841 AATAAAGACA AACCGCTGGG TGCCGTAGCG CTGAAGTCTT ACGAGGAAGA GTTGGCGAAA
     3901 GATCCACGTA TTGCCGCCAC TATGGAAAAC GCCCAGAAAG GTGAAATCAT GCCGAACATC
     3961 CCGCAGATGT CCGCTTTCTG GTATGCCGTG CGTACTGCGG TGATCAACGC CGCCAGCGGT
     4021 CGTCAGACTG TCGATGAAGC CCTGAAAGAC GCGCAGACTA ATTCGAGCTC GAACAACAAC
     4081 AACAATAACA ATAACAACAA CCTCGGGATC GAGGAAAACC TGTACTTCCA ATCCAATGCA
     4141 TGGAGCCACC CGCAGTTCGA AAAAGGTGGC AGCGGTTCTA TGTTTTTGCA AAGACCTAAA
     4201 CCTTACAGCG ATGAAAGTTT AGAAAGTTTC TTTATCCGAG TGGCTAACAA AAATGGCTAC
     4261 GGTGATGTCC ATCGCTTCCT AGAAGCCACT AAACGATTCC TTCAAGACAT TGACCATAAT
     4321 GGCTATCAAA CCTTTCCGAC TGATATAACT CGGATAAACC CATACTCAGC TAAAAACAGT
     4381 TCCAGCGCAC GAACTGCGTC ATTCCTGAAA CTTGCACAAT TGACATTTAA TGAACCGCCA
     4441 GAGCTACTTG GGTTGGCAAT TAACAGAACA AACATGAAAT ACTCGCCGTC AACTAGCGCG
     4501 GTTGTTAGAG GTGCAGAAGT CTTTCCTCGC AGTTTACTAC GGACGCACTC CATCCCCTGC
     4561 TGTCCTTTGT GTCTGCGAGA AAATGGCTAC GCCTCCTACC TTTGGCACTT TCAGGGGTAC
     4621 GAATACTGCC ACAGCCATAA CGTACCTTTA ATTACCACTT GTAGCTGTGG TAAGGAGTTT
     4681 GACTACCGAG TATCTGGGTT AAAGGGCATT TGCTGCAAAT GCAAGGAGCC TATCACCTTA
     4741 ACCAGCAGGG AGAACGGTCA TGAGGCAGCG TGTACTGTTT CAAACTGGCT TGCTGGCCAT
     4801 GAATCTAAAC CTCTGCCAAA TCTTCCTAAA AGCTACCGAT GGGGTTTAGT TCATTGGTGG
     4861 ATGGGTATTA AAGATAGCGA GTTCGATCAC TTTTCGTTCG TTCAATTTTT CTCAAACTGG
     4921 CCAAGGTCAT TCCACTCGAT AATCGAAGAT GAAGTAGAGT TCAACCTTGA GCATGCTGTT
     4981 GTCAGCACGT CTGAATTACG ACTAAAAGAT CTTCTTGGTC GATTGTTTTT CGGTTCAATT
     5041 CGGTTACCTG AGCGGAATCT TCAACACAAT ATCATCCTTG GTGAGCTTCT CTGCTATTTA
     5101 GAAAATCGTT TATGGCAAGA CAAGGGATTA ATCGCCAACC TCAAAATGAA CGCGTTAGAG
     5161 GCGACTGTAA TGTTAAATTG TAGCCTCGAT CAGATTGCAT CAATGGTTGA ACAACGCATC
     5221 TTGAAGCCAA ATCGAAAAAG CAAGCCCAAC AGCCCTCTTG ATGTTACCGA TTATCTATTT
     5281 CATTTCGGCG ATATTTTCTG TCTTTGGTTA GCTGAGTTCC AAAGCGATGA GTTTAACCGT
     5341 TCGTTTTATG TGTCGAGGTG GTAATAGCAT TGGAAGTGGA TAACGGATCC GCGATCGCGG
     5401 CGCGCCACCT GGTGGCCGGC CGGTACCACG CGTGCGCGCT GATCCGGCTG CTAACAAAGC
     5461 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG
     5521 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC
     5581 ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA
     5641 GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA
     5701 ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT
     5761 GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT
     5821 CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT
     5881 GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT
     5941 GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT
     6001 AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC
     6061 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA
     6121 TAACCCTGAT AAATGCTTCA ATAACATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC
     6181 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA
     6241 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA
     6301 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG
     6361 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA
     6421 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC
     6481 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC
     6541 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA
     6601 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG
     6661 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA
     6721 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA
     6781 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC
     6841 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA
     6901 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA
     6961 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG
     7021 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA
     7081 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
     7141 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTC
//