LOCUS       pSL0426_(CRISPR_with_sp 3739 bp ds-DNA     circular     10-JUL-2019
DEFINITION  .
KEYWORDS    "creator:Novagen (EMD Millipore)" "marker:CmR"                     
            "organism:Escherichia coli"
FEATURES             Location/Qualifiers
     misc_feature    complement(3195..3739)
                     /label="p15A ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(225..1307)
                     /label="LacI"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1582..1609
                     /label="Vch CRISPR repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(2567..2669)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1907..2566)
                     /label="CmR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1522..1549
                     /label="Vch CRISPR repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1431..1449
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1639..1686
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1308..1385)
                     /label="LacI promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1450..1474
                     /label="Lac operator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     guide sequence  1550..1581
                     /label="spc3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 GAAGATCATC TTATTAATCA GATAAAATAT TTCTAGATTT CAGTGCAATT TATCTCTTCA
       61 AATGTAGCAC CTGAAGTCAG CCCCATACGA TATAAGTTGT AATTCTCATG TTAGTCATGC
      121 CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC
      181 CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC
      241 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
      301 GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA
      361 TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC
      421 AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG
      481 GTATCGTCGT ATCCCACTAC CGAGATGTCC GCACCAACGC GCAGCCCGGA CTCGGTAATG
      541 GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG
      601 CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC
      661 CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC
      721 AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT
      781 GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG
      841 ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC
      901 ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC
      961 GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC
     1021 ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC
     1081 GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC
     1141 AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT
     1201 TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA
     1261 GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG
     1321 AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG
     1381 GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAAT TAATACGACT
     1441 CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTGTA GAAATAATTT TGTTTAACTT
     1501 TAATAAGGAG ATATACCATG GGTGAACTGC CGAGTAGGTA GCTGATAACT TACAGGACGC
     1561 TTTGGCTTCA TTGCTTTTCA GGTGAACTGC CGAGTAGGTA GCTGATAACC CTAGGCTGCT
     1621 GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1681 TTTTTGCTGA AACCTCAGGC ATTTGAGAAG CACACGGTCA CACTGCTTCC GGTAGTCAAT
     1741 AAACCGGTAA ACCAGCAATA GACATAAGCG GCTATTTAAC GACCCTGCCC TGAACCGACG
     1801 ACCGGGTCGA ATTTGCTTTC GAATTTCTGC CATTCATCCG CTTATTATCA CTTATTCAGG
     1861 CGTAGCAACC AGGCGTTTAA GGGCACCAAT AACTGCCTTA AAAAAATTAC GCCCCGCCCT
     1921 GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC
     1981 AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT
     2041 ATTTGCCCAT AGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA
     2101 AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT
     2161 TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA
     2221 ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT
     2281 GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG
     2341 CCATACGGAA CTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT
     2401 AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG
     2461 TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC
     2521 ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG
     2581 CTCCTGAAAA TCTCGATAAC TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT
     2641 GAAAGTTGGA ACCTCTTACG TGCCGATCAA CGTCTCATTT TCGCCAAAAG TTGGCCCAGG
     2701 GCTTCCCGGT ATCAACAGGG ACACCAGGAT TTATTTATTC TGCGAAGTGA TCTTCCGTCA
     2761 CAGGTATTTA TTCGGCGCAA AGTGCGTCGG GTGATGCTGC CAACTTACTG ATTTAGTGTA
     2821 TGATGGTGTT TTTGAGGTGC TCCAGTGGCT TCTGTTTCTA TCAGCTGTCC CTCCTGTTCA
     2881 GCTACTGACG GGGTGGTGCG TAACGGCAAA AGCACCGCCG GACATCAGCG CTAGCGGAGT
     2941 GTATACTGGC TTACTATGTT GGCACTGATG AGGGTGTCAG TGAAGTGCTT CATGTGGCAG
     3001 GAGAAAAAAG GCTGCACCGG TGCGTCAGCA GAATATGTGA TACAGGATAT ATTCCGCTTC
     3061 CTCGCTCACT GACTCGCTAC GCTCGGTCGT TCGACTGCGG CGAGCGGAAA TGGCTTACGA
     3121 ACGGGGCGGA GATTTCCTGG AAGATGCCAG GAAGATACTT AACAGGGAAG TGAGAGGGCC
     3181 GCGGCAAAGC CGTTTTTCCA TAGGCTCCGC CCCCCTGACA AGCATCACGA AATCTGACGC
     3241 TCAAATCAGT GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCTGGCG
     3301 GCTCCCTCGT GCGCTCTCCT GTTCCTGCCT TTCGGTTTAC CGGTGTCATT CCGCTGTTAT
     3361 GGCCGCGTTT GTCTCATTCC ACGCCTGACA CTCAGTTCCG GGTAGGCAGT TCGCTCCAAG
     3421 CTGGACTGTA TGCACGAACC CCCCGTTCAG TCCGACCGCT GCGCCTTATC CGGTAACTAT
     3481 CGTCTTGAGT CCAACCCGGA AAGACATGCA AAAGCACCAC TGGCAGCAGC CACTGGTAAT
     3541 TGATTTAGAG GAGTTAGTCT TGAAGTCATG CGCCGGTTAA GGCTAAACTG AAAGGACAAG
     3601 TTTTGGTGAC TGCGCTCCTC CAAGCCAGTT ACCTCGGTTC AAAGAGTTGG TAGCTCAGAG
     3661 AACCTTCGAA AAACCGCCCT GCAAGGCGGT TTTTTCGTTT TCAGAGCAAG AGATTACGCG
     3721 CAGACCAAAA CGATCTCAA
//