LOCUS       Exported               13950 bp ds-DNA     circular SYN 19-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13950)
  AUTHORS   Yosuke
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 16, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13950
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        153..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        457..660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             743..6826
                     /codon_start=1
                     /label=Ex1-48.3Flag
                     /translation="MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPL
                     QVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVN
                     QLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVR
                     RIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPV
                     PVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEA
                     IYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFH
                     LWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDM
                     QTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDC
                     FILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTND
                     KFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKF
                     TLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYG
                     DHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLS
                     VTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILH
                     SSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSK
                     AIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLS
                     LHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRL
                     KVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSG
                     TSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQK
                     AIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQG
                     LLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQE
                     LQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFI
                     PGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQ
                     LCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQV
                     KAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSII
                     KNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYC
                     IQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLS
                     ITDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVNGSAEIMSLVLAE
                     SSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAALTKEKDQLSQSIV
                     MYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQVPHIEALLKRKLS
                     EQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLT
                     TDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKPIGDPVFARDLLT
                     FPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFG
                     GLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYL
                     RTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVETTDCPVPPKRMRRE
                     ATRQNRIITKTDVDYKDHDGDYKDHDIDYKDDDDK"
     CDS             743..6757
                     /codon_start=1
                     /label=SMCHD1 (Ex1-48)
                     /translation="MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPL
                     QVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVN
                     QLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVR
                     RIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPV
                     PVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEA
                     IYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFH
                     LWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDM
                     QTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDC
                     FILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTND
                     KFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKF
                     TLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYG
                     DHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLS
                     VTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILH
                     SSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSK
                     AIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLS
                     LHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRL
                     KVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSG
                     TSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQK
                     AIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQG
                     LLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQE
                     LQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFI
                     PGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQ
                     LCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQV
                     KAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSII
                     KNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYC
                     IQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLS
                     ITDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVNGSAEIMSLVLAE
                     SSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAALTKEKDQLSQSIV
                     MYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQVPHIEALLKRKLS
                     EQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLT
                     TDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKPIGDPVFARDLLT
                     FPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFG
                     GLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYL
                     RTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVETTDCPVPPKRMRRE
                     ATRQNRIITKTDV"
     primer_bind     6339..6368
                     /label=S81
     CDS             6623..6718
                     /codon_start=1
                     /label=NLS
                     /translation="MTPIRKCNDSLRHSPKVETTDCPVPPKRMRRE"
     primer_bind     6678..6698
                     /label=qPCR F
     CDS             6701..6712
                     /codon_start=1
                     /label=consensus
                     /translation="KRMR"
     primer_bind     complement(6737..6835)
                     /label=S82 SalI-stop-3xFlag-SMCHD1
     CDS             6758..6823
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(6763..6783)
                     /label=S167 Flag R
     primer_bind     complement(6798..6818)
                     /label=S168 Flag R
     misc_feature    6833..7421
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7304..7315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7435..7934
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             7955..8554
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             8692..8925
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8997..9118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9137..9272
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9305..9323)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9333..9349)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9494..9949
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9978..10082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10083..10943
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11114..11702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11990..12011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12026..12056
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12064..12080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12088..12104
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12125..12143
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             12399..12579
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12623..12748
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13241..13474
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tcgacggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
       61 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      121 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      661 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      721 agacaccgac tctagaggat ccatggcagc ggcggacggc ggcgggcctg gtggggcctc
      781 tgtggggact gaggaggatg gcggaggcgt cggccacagg acggtgtact tgtttgatcg
      841 gcgcgaaaag gagtccgagc tcggggaccg gcctctgcag gtcggggagc gctcggacta
      901 cgcgggattt cgcgcgtgtg tgtgtcagac acttggcatt tcacctgaag aaaaatttgt
      961 tattacaaca acaagtagga aagaaattac ctgtgataat tttgatgaaa ctgttaaaga
     1021 tggagtcacc ttatacctgc tacagtcggt caatcagtta ctactgacag ctacgaaaga
     1081 acgaattgac ttcttacctc actatgacac actggttaaa agtggcatgt atgaatatta
     1141 tgccagtgaa ggacaaaatc ctttgccatt tgctcttgcg gaattaattg acaattcatt
     1201 gtctgctact tctcgtaaca ttggggttag aagaatacag atcaaattgc tttttgatga
     1261 aacacaagga aaacctgctg ttgcagtgat agataatgga agaggaatga cctctaaaca
     1321 gcttaacaac tgggccgtgt ataggttgtc aaaattcaca aggcaaggtg actttgaaag
     1381 tgatcattca ggatatgttc gtccagtacc agtgccacgc agtttaaata gtgatatttc
     1441 ctattttggt gttgggggca agcaagctgt cttctttgtt ggacaatcag ccagaatgat
     1501 aagcaaacct gcagattccc aagatgttca cgagcttgtg ctttctaaag aagattttga
     1561 gaagaaggag aaaaataaag aggcaatata tagtggatat attagaaaca gaaagccctc
     1621 tgattctgtt cacattacaa atgatgatga aagatttcta catcatctta tcatagagga
     1681 gaaggaaaaa gatagcttta ctgctgtggt tatcacaggg gtacaaccag aacacataca
     1741 gtacttgaaa aattatttcc acctttggac acgacagtta gcgcatattt atcactacta
     1801 tattcatggc ccaaaaggaa atgaaatacg aacatcaaaa gaagttgaac ctttcaacaa
     1861 tattgatatt gaaatttcta tgtttgaaaa agggaaggta cctaagattg tcaacctaag
     1921 ggaaatacaa gacgacatgc agacgttgta tgtaaacaca gcagctgata gttttgaatt
     1981 caaagctcat gttgaaggag atggtgtagt ggaagggatt atccgttatc atccattctt
     2041 atatgataga gaaacttacc ctgatgatcc atgctttcca tcaaaattaa aagatgaaga
     2101 tgatgaagat gattgtttca tacttgagaa agcagctaga gggaaaaggc ctatttttga
     2161 atgtttttgg aatggacgat taataccata tacatcagtt gaagactttg actggtgtac
     2221 tcctcctaag aagagagggc ttgcaccaat tgaatgctac aataggatat ctggtgcatt
     2281 attcactaat gacaaattcc aggtcagcac aaataaattg acgtttatgg atcttgagct
     2341 aaaattgaaa gataagaaca ccctttttac aaggatttta aatggacagg aacagcgaat
     2401 gaaaattgac agagaatttg ctttgtggct gaaggactgt catgagaagt atgataaaca
     2461 aataaaattt acacttttta agggagtaat tacacgtcct gatcttcctt ctaaaaagca
     2521 aggtccctgg gcaacatatg cagcaataga atgggatgga aagatataca aagcaggaca
     2581 gctggtcaag acaatcaaga cacttcccct cttttatgga agcatagtaa gattttttct
     2641 ttatggcgat catgatggag aagtatatgc tacaggagga gaggttcaaa ttgcaatgga
     2701 acctcaggca ctatatgatg aagtaagaac tgtgccaatt gcaaagctgg ataggacagt
     2761 tgctgagaaa gctgttaaaa aatatgtaga agatgaaatg gcaaggctcc ctgatagatt
     2821 gtcagtaact tggcctgaag gagatgaatt attgcctaat gaggttaggc ctgctggaac
     2881 ccctattggt gcgttaagaa ttgaaatact gaataaaaaa ggggaagcaa tgcaaaagct
     2941 tccaggaaca agccatggag ggtcaaagaa actcctggtt gagctcaaag ttattttaca
     3001 ttcttcaagt ggaaataaag agattatttc gcatattagt caacatggag gaaaatggcc
     3061 ttactggttt aaaaaaatgg aaaatattca gaagttgggg aattatacct tgaaattaca
     3121 agttgtgttg aatgaaagta atgcagacac ttatgcagga agaccactac catctaaagc
     3181 aattaagttt tctgttaaag agggtaagcc agagaaattt tcatttggtc ttctggatct
     3241 tccttttcgt gttggagttc catttaatat ccctctggag tttcaggatg aatttggtca
     3301 taccagtcaa ctagtaactg atattcagcc agttcttgaa gcaagtggtt tatctttaca
     3361 ttatgaagaa ataaccaaag gaccaaattg tgtaattcga ggtgttacag ccaagggccc
     3421 tgtaaactct tgtcaaggca agaattataa tctgaaggtt actctgcctg gcttaaaaga
     3481 agactcacag attttgaaaa ttagattact acctggtcac cctcgtcgac tgaaagtgaa
     3541 acctgattct gaaattttag ttatagaaaa tggaacagct ttcccatttc aggtggaagt
     3601 tttagatgaa tcagacaaca taacagcaca accaaaattg attgttcatt gtaagttttc
     3661 aggtgctcca aaccttccag tctatgttgt agattgcagt agttctggaa ccagtatttt
     3721 aacaggatct gcaattcaag ttcagaatat taaaaaagac cagacgctta aagcaagaat
     3781 tgaaatacct agttgtaaag atgtggcacc tgtggagaag actattaagt tgcttcccag
     3841 tagccatgtt gcaagactac aaatattcag tgtagaagga caaaaggcaa ttcagatcaa
     3901 acatcaggat gaggttaatt ggatagcggg tgatattatg cataatctta tttttcaaat
     3961 gtatgatgaa ggagaaagag aaatcaatat aacatcagct ttagcagaaa aaattaaagt
     4021 taattggact cctgagatta acaaagaaca cttgctacag ggtctgcttc ctgatgtgca
     4081 agtaccaaca tctgtaaaag atatgcgcta ttgccaggtt tcattccaag atgatcatgt
     4141 gtctttggaa agtgcgttta cagtaagacc acttcctgat gaacctaaac atttaaaatg
     4201 tgaaatgaaa ggaggaaaaa cagtacagat gggccaagag cttcaaggag aagtagttat
     4261 aataattaca gatcagtacg gaaatcagat tcaagcattt tcaccaagtt ctttatcttc
     4321 tttgtcaatt gctggggttg gacttgatag ctcaaatttg aaaacaacct ttcaggaaaa
     4381 cacacagagt ataagtgtaa gaggcatcaa atttattcca ggtcctcctg gaaataagga
     4441 tctttgtttt acttggcgtg agttttctga ctttattcga gtgcaactaa tttctggacc
     4501 tcctgctaaa cttctcctta tagactggcc agaactaaag gagtccattc cagtgattaa
     4561 tggaagagat ttacagaacc ctattattgt tcaactttgt gatcagtggg ataatccagc
     4621 accggtacaa catgttaaaa taagtcttac aaaagctagc aatttaaagc tcatgccttc
     4681 aaaccaacag cataaaacag atgagaaagg cagggctaat ttgggagtat tcagtgtttt
     4741 tgcccctagg ggagagcata ctcttcaggt taaagccatc tataacaaaa gtatcataga
     4801 aggacctata attaagttaa tgattcttcc agacccagaa aaacccgttc gtctcaatgt
     4861 taaatatgac aaagatgcat ccttcttagc agggggtctt ttcactgatt ttatgattag
     4921 tgttatttct gaagatgaca gtatcattaa aaacattaat ccagcacgta tttccatgaa
     4981 aatgtggaag ctgtctacca gtgggaaccg acccccagca aatgcagaaa catttagttg
     5041 taataaaata aaagataatg acaaagaaga tggctgcttc tatttcaggg ataaagtaat
     5101 tcctaataaa gtggggacat attgtatcca gtttggtttt atgatggata aaacaaatat
     5161 tctcaacagt gaacaggtta tagttgaagt cctgcctaat caacctgtga agttagtacc
     5221 taaaattaaa ccacctacac cagctgtttc aaatgttcgc tcagttgcca gtaggacctt
     5281 ggtcagagat ctacatctta gtatcacgga tgactacgac aaccatactg gaattgattt
     5341 ggttggcact ataatagcca ccattaaagg ctctaatgag gaagatactg ataccccact
     5401 ttttattggg aaagttagaa cacttgaatt ccccttcgtg aatggttcgg ctgaaatcat
     5461 gagtctggtg ctggcagaaa gtagtcctgg aagggatagt actgaatatt ttattgtatt
     5521 tgagccccgg ctaccacttt tatcaagaac cttagaacca tatatcctac cgttcatgtt
     5581 ttacaatgat gttaagaagc agcaacaaat ggcagcactt acaaaagaaa aggaccaatt
     5641 atctcagtct attgttatgt ataaaagttt atttgaagcc agccaacagc ttcttaatga
     5701 aatgaaatgt caagttgaag aagcaagatt aaaagaggcc caattgcgaa atgaactaaa
     5761 aatacataat attgacattc ctacaacaca acaggtgcca cacattgaag cacttctgaa
     5821 aagaaagcta tcagaacaag aagaactgaa gaaaaaacct agaagatcgt gtactcttcc
     5881 aaactatact aaaggcagtg gagatgtttt gggaaagatt gcacatctag cacaaattga
     5941 agatgataga gctgcgatgg ttatttcttg gcatctggca agtgacatgg actgtgtagt
     6001 caccctaacc actgacgctg cacgtcgtat ctatgatgaa acccaaggtc gtcagcaggt
     6061 gttgcccctt gattctattt acaagaagac tcttccagat tggaaaagat ctctacctca
     6121 tttccgaaat ggaaaattgt attttaaacc cattggagat ccagtctttg ctcgagactt
     6181 gttaacattt ccagataatg tagaacattg tgaaacagta tttggtatgc tgttaggaga
     6241 caccattatt ttggataatc tggatgcggc caatcattat agaaaagagg ttgttaaaat
     6301 tacacactgt cctacactgc tgaccagaga tggagatcga attcgaagta atggaaagtt
     6361 tgggggcctt cagaataaag ctcctccaat ggataaactt cggggaatgg tatttggagc
     6421 tccagttcca aaacagtgtc tgatcttagg ggaacaaata gatcttcttc agcagtatcg
     6481 ttctgctgtg tgcaaactag acagtgtgaa taaggatctt aacagtcaat tagagtacct
     6541 tcgcactccg gatatgagga agaaaaagca agaacttgat gaacatgaga aaaatctcaa
     6601 actaatagag gaaaaactag gtatgactcc catacgtaag tgtaatgact cattgcgtca
     6661 ttcaccaaag gttgagacga cagattgtcc agttcctcct aaaagaatga gacgagaagc
     6721 tacaagacaa aataggatta taaccaaaac agatgtagac tacaaagacc atgacggtga
     6781 ttataaagat catgacatcg attacaagga tgacgatgac aagtgagtcg acaatcaacc
     6841 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6901 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6961 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7021 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7081 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7141 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7201 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     7261 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7321 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7381 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc taccgggtag
     7441 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     7501 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     7561 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     7621 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     7681 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     7741 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     7801 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     7861 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     7921 ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg
     7981 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     8041 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     8101 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     8161 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     8221 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     8281 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     8341 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     8401 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     8461 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     8521 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     8581 gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta agaccaatga
     8641 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8701 tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8761 accagatctg agcctgggag ctctctggct gcctagggaa cccactgctt aagcctcaat
     8821 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8881 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     8941 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     9001 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9061 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9121 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     9181 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     9241 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga cgtacccaat
     9301 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     9361 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     9421 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     9481 ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9541 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9601 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9661 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9721 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9781 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9841 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9901 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccaggtggca
     9961 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    10021 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    10081 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    10141 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    10201 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    10261 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    10321 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    10381 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    10441 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    10501 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10561 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10621 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10681 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10741 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10801 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10861 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10921 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    11041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    11101 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    11161 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    11221 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
    11281 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    11341 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    11401 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    11461 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    11521 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    11581 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    11641 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11701 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11761 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11821 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    11881 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    11941 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    12001 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    12061 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    12121 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
    12181 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
    12241 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
    12301 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
    12361 agatattgta tttaagtgcc tagctcgata caataaacgg gtctctctgg ttagaccaga
    12421 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    12481 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    12541 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggacct
    12601 gaaagcgaaa gggaaaccag agctctctcg acgcaggact cggcttgctg aagcgcgcac
    12661 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
    12721 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
    12781 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
    12841 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
    12901 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
    12961 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
    13021 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
    13081 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
    13141 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
    13201 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
    13261 tcttgggagc agcaggaagc actatgggcg cagcctcaat gacgctgacg gtacaggcca
    13321 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
    13381 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
    13441 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
    13501 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
    13561 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    13621 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    13681 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    13741 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    13801 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    13861 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
    13921 agacagatcc attcgattag tgaacggatc
//
