LOCUS       Exported               13854 bp ds-DNA     circular SYN 18-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13854)
  AUTHORS   Yosuke
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 16, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13854
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        153..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        457..660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             743..6730
                     /codon_start=1
                     /label=Ex1-46.47A-48-3Flag
                     /translation="MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPL
                     QVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVN
                     QLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVR
                     RIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPV
                     PVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEA
                     IYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFH
                     LWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDM
                     QTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDC
                     FILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTND
                     KFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKF
                     TLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYG
                     DHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLS
                     VTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILH
                     SSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSK
                     AIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLS
                     LHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRL
                     KVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSG
                     TSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQK
                     AIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQG
                     LLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQE
                     LQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFI
                     PGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQ
                     LCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQV
                     KAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSII
                     KNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYC
                     IQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLS
                     ITDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVNGSAEIMSLVLAE
                     SSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAALTKEKDQLSQSIV
                     MYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQVPHIEALLKRKLS
                     EQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLT
                     TDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKPIGDPVFARDLLT
                     FPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFG
                     GLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYL
                     RTPDMRKKKQELDEHEKNLKLIEEKLGATRQNRIITKTDVDYKDHDGDYKDHDIDYKDD
                     DDK"
     CDS             6623..6661
                     /codon_start=1
                     /label=47A-48
                     /translation="ATRQNRIITKTDV"
     CDS             6662..6727
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6737..7325
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7208..7219)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7339..7838
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             7859..8458
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             8596..8829
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8901..9022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9041..9176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9209..9227)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9237..9253)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9398..9853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9882..9986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9987..10847
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11018..11606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11894..11915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11930..11960
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11968..11984
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11992..12008
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12029..12047
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             12303..12483
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12527..12652
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13145..13378
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tcgacggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
       61 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      121 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      661 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      721 agacaccgac tctagaggat ccatggcagc ggcggacggc ggcgggcctg gtggggcctc
      781 tgtggggact gaggaggatg gcggaggcgt cggccacagg acggtgtact tgtttgatcg
      841 gcgcgaaaag gagtccgagc tcggggaccg gcctctgcag gtcggggagc gctcggacta
      901 cgcgggattt cgcgcgtgtg tgtgtcagac acttggcatt tcacctgaag aaaaatttgt
      961 tattacaaca acaagtagga aagaaattac ctgtgataat tttgatgaaa ctgttaaaga
     1021 tggagtcacc ttatacctgc tacagtcggt caatcagtta ctactgacag ctacgaaaga
     1081 acgaattgac ttcttacctc actatgacac actggttaaa agtggcatgt atgaatatta
     1141 tgccagtgaa ggacaaaatc ctttgccatt tgctcttgcg gaattaattg acaattcatt
     1201 gtctgctact tctcgtaaca ttggggttag aagaatacag atcaaattgc tttttgatga
     1261 aacacaagga aaacctgctg ttgcagtgat agataatgga agaggaatga cctctaaaca
     1321 gcttaacaac tgggccgtgt ataggttgtc aaaattcaca aggcaaggtg actttgaaag
     1381 tgatcattca ggatatgttc gtccagtacc agtgccacgc agtttaaata gtgatatttc
     1441 ctattttggt gttgggggca agcaagctgt cttctttgtt ggacaatcag ccagaatgat
     1501 aagcaaacct gcagattccc aagatgttca cgagcttgtg ctttctaaag aagattttga
     1561 gaagaaggag aaaaataaag aggcaatata tagtggatat attagaaaca gaaagccctc
     1621 tgattctgtt cacattacaa atgatgatga aagatttcta catcatctta tcatagagga
     1681 gaaggaaaaa gatagcttta ctgctgtggt tatcacaggg gtacaaccag aacacataca
     1741 gtacttgaaa aattatttcc acctttggac acgacagtta gcgcatattt atcactacta
     1801 tattcatggc ccaaaaggaa atgaaatacg aacatcaaaa gaagttgaac ctttcaacaa
     1861 tattgatatt gaaatttcta tgtttgaaaa agggaaggta cctaagattg tcaacctaag
     1921 ggaaatacaa gacgacatgc agacgttgta tgtaaacaca gcagctgata gttttgaatt
     1981 caaagctcat gttgaaggag atggtgtagt ggaagggatt atccgttatc atccattctt
     2041 atatgataga gaaacttacc ctgatgatcc atgctttcca tcaaaattaa aagatgaaga
     2101 tgatgaagat gattgtttca tacttgagaa agcagctaga gggaaaaggc ctatttttga
     2161 atgtttttgg aatggacgat taataccata tacatcagtt gaagactttg actggtgtac
     2221 tcctcctaag aagagagggc ttgcaccaat tgaatgctac aataggatat ctggtgcatt
     2281 attcactaat gacaaattcc aggtcagcac aaataaattg acgtttatgg atcttgagct
     2341 aaaattgaaa gataagaaca ccctttttac aaggatttta aatggacagg aacagcgaat
     2401 gaaaattgac agagaatttg ctttgtggct gaaggactgt catgagaagt atgataaaca
     2461 aataaaattt acacttttta agggagtaat tacacgtcct gatcttcctt ctaaaaagca
     2521 aggtccctgg gcaacatatg cagcaataga atgggatgga aagatataca aagcaggaca
     2581 gctggtcaag acaatcaaga cacttcccct cttttatgga agcatagtaa gattttttct
     2641 ttatggcgat catgatggag aagtatatgc tacaggagga gaggttcaaa ttgcaatgga
     2701 acctcaggca ctatatgatg aagtaagaac tgtgccaatt gcaaagctgg ataggacagt
     2761 tgctgagaaa gctgttaaaa aatatgtaga agatgaaatg gcaaggctcc ctgatagatt
     2821 gtcagtaact tggcctgaag gagatgaatt attgcctaat gaggttaggc ctgctggaac
     2881 ccctattggt gcgttaagaa ttgaaatact gaataaaaaa ggggaagcaa tgcaaaagct
     2941 tccaggaaca agccatggag ggtcaaagaa actcctggtt gagctcaaag ttattttaca
     3001 ttcttcaagt ggaaataaag agattatttc gcatattagt caacatggag gaaaatggcc
     3061 ttactggttt aaaaaaatgg aaaatattca gaagttgggg aattatacct tgaaattaca
     3121 agttgtgttg aatgaaagta atgcagacac ttatgcagga agaccactac catctaaagc
     3181 aattaagttt tctgttaaag agggtaagcc agagaaattt tcatttggtc ttctggatct
     3241 tccttttcgt gttggagttc catttaatat ccctctggag tttcaggatg aatttggtca
     3301 taccagtcaa ctagtaactg atattcagcc agttcttgaa gcaagtggtt tatctttaca
     3361 ttatgaagaa ataaccaaag gaccaaattg tgtaattcga ggtgttacag ccaagggccc
     3421 tgtaaactct tgtcaaggca agaattataa tctgaaggtt actctgcctg gcttaaaaga
     3481 agactcacag attttgaaaa ttagattact acctggtcac cctcgtcgac tgaaagtgaa
     3541 acctgattct gaaattttag ttatagaaaa tggaacagct ttcccatttc aggtggaagt
     3601 tttagatgaa tcagacaaca taacagcaca accaaaattg attgttcatt gtaagttttc
     3661 aggtgctcca aaccttccag tctatgttgt agattgcagt agttctggaa ccagtatttt
     3721 aacaggatct gcaattcaag ttcagaatat taaaaaagac cagacgctta aagcaagaat
     3781 tgaaatacct agttgtaaag atgtggcacc tgtggagaag actattaagt tgcttcccag
     3841 tagccatgtt gcaagactac aaatattcag tgtagaagga caaaaggcaa ttcagatcaa
     3901 acatcaggat gaggttaatt ggatagcggg tgatattatg cataatctta tttttcaaat
     3961 gtatgatgaa ggagaaagag aaatcaatat aacatcagct ttagcagaaa aaattaaagt
     4021 taattggact cctgagatta acaaagaaca cttgctacag ggtctgcttc ctgatgtgca
     4081 agtaccaaca tctgtaaaag atatgcgcta ttgccaggtt tcattccaag atgatcatgt
     4141 gtctttggaa agtgcgttta cagtaagacc acttcctgat gaacctaaac atttaaaatg
     4201 tgaaatgaaa ggaggaaaaa cagtacagat gggccaagag cttcaaggag aagtagttat
     4261 aataattaca gatcagtacg gaaatcagat tcaagcattt tcaccaagtt ctttatcttc
     4321 tttgtcaatt gctggggttg gacttgatag ctcaaatttg aaaacaacct ttcaggaaaa
     4381 cacacagagt ataagtgtaa gaggcatcaa atttattcca ggtcctcctg gaaataagga
     4441 tctttgtttt acttggcgtg agttttctga ctttattcga gtgcaactaa tttctggacc
     4501 tcctgctaaa cttctcctta tagactggcc agaactaaag gagtccattc cagtgattaa
     4561 tggaagagat ttacagaacc ctattattgt tcaactttgt gatcagtggg ataatccagc
     4621 accggtacaa catgttaaaa taagtcttac aaaagctagc aatttaaagc tcatgccttc
     4681 aaaccaacag cataaaacag atgagaaagg cagggctaat ttgggagtat tcagtgtttt
     4741 tgcccctagg ggagagcata ctcttcaggt taaagccatc tataacaaaa gtatcataga
     4801 aggacctata attaagttaa tgattcttcc agacccagaa aaacccgttc gtctcaatgt
     4861 taaatatgac aaagatgcat ccttcttagc agggggtctt ttcactgatt ttatgattag
     4921 tgttatttct gaagatgaca gtatcattaa aaacattaat ccagcacgta tttccatgaa
     4981 aatgtggaag ctgtctacca gtgggaaccg acccccagca aatgcagaaa catttagttg
     5041 taataaaata aaagataatg acaaagaaga tggctgcttc tatttcaggg ataaagtaat
     5101 tcctaataaa gtggggacat attgtatcca gtttggtttt atgatggata aaacaaatat
     5161 tctcaacagt gaacaggtta tagttgaagt cctgcctaat caacctgtga agttagtacc
     5221 taaaattaaa ccacctacac cagctgtttc aaatgttcgc tcagttgcca gtaggacctt
     5281 ggtcagagat ctacatctta gtatcacgga tgactacgac aaccatactg gaattgattt
     5341 ggttggcact ataatagcca ccattaaagg ctctaatgag gaagatactg ataccccact
     5401 ttttattggg aaagttagaa cacttgaatt ccccttcgtg aatggttcgg ctgaaatcat
     5461 gagtctggtg ctggcagaaa gtagtcctgg aagggatagt actgaatatt ttattgtatt
     5521 tgagccccgg ctaccacttt tatcaagaac cttagaacca tatatcctac cgttcatgtt
     5581 ttacaatgat gttaagaagc agcaacaaat ggcagcactt acaaaagaaa aggaccaatt
     5641 atctcagtct attgttatgt ataaaagttt atttgaagcc agccaacagc ttcttaatga
     5701 aatgaaatgt caagttgaag aagcaagatt aaaagaggcc caattgcgaa atgaactaaa
     5761 aatacataat attgacattc ctacaacaca acaggtgcca cacattgaag cacttctgaa
     5821 aagaaagcta tcagaacaag aagaactgaa gaaaaaacct agaagatcgt gtactcttcc
     5881 aaactatact aaaggcagtg gagatgtttt gggaaagatt gcacatctag cacaaattga
     5941 agatgataga gctgcgatgg ttatttcttg gcatctggca agtgacatgg actgtgtagt
     6001 caccctaacc actgacgctg cacgtcgtat ctatgatgaa acccaaggtc gtcagcaggt
     6061 gttgcccctt gattctattt acaagaagac tcttccagat tggaaaagat ctctacctca
     6121 tttccgaaat ggaaaattgt attttaaacc cattggagat ccagtctttg ctcgagactt
     6181 gttaacattt ccagataatg tagaacattg tgaaacagta tttggtatgc tgttaggaga
     6241 caccattatt ttggataatc tggatgcggc caatcattat agaaaagagg ttgttaaaat
     6301 tacacactgt cctacactgc tgaccagaga tggagatcga attcgaagta atggaaagtt
     6361 tgggggcctt cagaataaag ctcctccaat ggataaactt cggggaatgg tatttggagc
     6421 tccagttcca aaacagtgtc tgatcttagg ggaacaaata gatcttcttc agcagtatcg
     6481 ttctgctgtg tgcaaactag acagtgtgaa taaggatctt aacagtcaat tagagtacct
     6541 tcgcactccg gatatgagga agaaaaagca agaacttgat gaacatgaga aaaatctcaa
     6601 actaatagag gaaaaactag gtgctacaag acaaaatagg attataacca aaacagatgt
     6661 agactacaaa gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga
     6721 tgacaagtga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6781 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6841 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6901 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6961 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7021 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7081 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7141 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7201 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7261 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7321 cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     7381 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     7441 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     7501 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     7561 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     7621 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     7681 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     7741 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     7801 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     7861 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     7921 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     7981 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     8041 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     8101 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     8161 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     8221 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     8281 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     8341 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     8401 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     8461 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg
     8521 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8581 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg
     8641 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctgcctag
     8701 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8761 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8821 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8881 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     8941 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9001 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     9061 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9121 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9181 cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     9241 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     9301 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     9361 tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta
     9421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     9481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9541 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     9841 acgtttacaa tttcccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9901 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9961 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    10021 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10081 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10141 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10201 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    10261 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10321 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10381 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10441 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10501 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    10561 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    10621 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10681 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10741 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10801 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    10861 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10921 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10981 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    11041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11161 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11701 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    11761 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    11821 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    11881 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    11941 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    12001 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    12061 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
    12121 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
    12181 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
    12241 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa
    12301 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    12361 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    12421 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    12481 gcagtggcgc ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag
    12541 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
    12601 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
    12661 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
    12721 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
    12781 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
    12841 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
    12901 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
    12961 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
    13021 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
    13081 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
    13141 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct
    13201 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
    13261 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
    13321 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
    13381 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
    13441 ggagtaataa atctctggaa cagattggaa tcacacgacc tggatggagt gggacagaga
    13501 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    13561 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    13621 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    13681 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    13741 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    13801 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatc
//
