LOCUS       Exported               13536 bp ds-DNA     circular SYN 27-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Yosuke
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 16, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        153..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        457..660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             743..6412
                     /codon_start=1
                     /label=p.Arg1868*-Flag
                     /translation="MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPL
                     QVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVN
                     QLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVR
                     RIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPV
                     PVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEA
                     IYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFH
                     LWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDM
                     QTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDC
                     FILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTND
                     KFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKF
                     TLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYG
                     DHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLS
                     VTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILH
                     SSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSK
                     AIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLS
                     LHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRL
                     KVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSG
                     TSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQK
                     AIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQG
                     LLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQE
                     LQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFI
                     PGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQ
                     LCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQV
                     KAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSII
                     KNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYC
                     IQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLS
                     ITDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVNGSAEIMSLVLAE
                     SSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAALTKEKDQLSQSIV
                     MYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQVPHIEALLKRKLS
                     EQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLT
                     TDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKPIGDPVFARDLLT
                     FPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIDYKDHDG
                     DYKDHDIDYKDDDDK"
     CDS             743..6343
                     /codon_start=1
                     /label=p.Arg1868*
                     /translation="MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPL
                     QVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVN
                     QLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVR
                     RIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPV
                     PVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEA
                     IYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFH
                     LWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDM
                     QTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDC
                     FILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTND
                     KFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKF
                     TLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYG
                     DHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLS
                     VTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILH
                     SSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSK
                     AIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLS
                     LHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRL
                     KVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSG
                     TSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQK
                     AIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQG
                     LLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQE
                     LQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFI
                     PGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQ
                     LCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQV
                     KAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSII
                     KNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYC
                     IQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLS
                     ITDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVNGSAEIMSLVLAE
                     SSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAALTKEKDQLSQSIV
                     MYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQVPHIEALLKRKLS
                     EQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLT
                     TDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKPIGDPVFARDLLT
                     FPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRI"
     primer_bind     4652..4681
                     /label=S34
     primer_bind     complement(6323..6421)
                     /label=S199
     CDS             6344..6409
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6419..7007
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6890..6901)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7021..7520
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             7541..8140
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             8278..8511
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8583..8704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8723..8858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8891..8909)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8919..8935)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9080..9535
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9564..9668
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9669..10529
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10700..11288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11576..11597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11612..11642
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11650..11666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11674..11690
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11711..11729
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             11985..12165
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12209..12334
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12827..13060
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tcgacggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
       61 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      121 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      661 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      721 agacaccgac tctagaggat ccatggcagc ggcggacggc ggcgggcctg gtggggcctc
      781 tgtggggact gaggaggatg gcggaggcgt cggccacagg acggtgtact tgtttgatcg
      841 gcgcgaaaag gagtccgagc tcggggaccg gcctctgcag gtcggggagc gctcggacta
      901 cgcgggattt cgcgcgtgtg tgtgtcagac acttggcatt tcacctgaag aaaaatttgt
      961 tattacaaca acaagtagga aagaaattac ctgtgataat tttgatgaaa ctgttaaaga
     1021 tggagtcacc ttatacctgc tacagtcggt caatcagtta ctactgacag ctacgaaaga
     1081 acgaattgac ttcttacctc actatgacac actggttaaa agtggcatgt atgaatatta
     1141 tgccagtgaa ggacaaaatc ctttgccatt tgctcttgcg gaattaattg acaattcatt
     1201 gtctgctact tctcgtaaca ttggggttag aagaatacag atcaaattgc tttttgatga
     1261 aacacaagga aaacctgctg ttgcagtgat agataatgga agaggaatga cctctaaaca
     1321 gcttaacaac tgggccgtgt ataggttgtc aaaattcaca aggcaaggtg actttgaaag
     1381 tgatcattca ggatatgttc gtccagtacc agtgccacgc agtttaaata gtgatatttc
     1441 ctattttggt gttgggggca agcaagctgt cttctttgtt ggacaatcag ccagaatgat
     1501 aagcaaacct gcagattccc aagatgttca cgagcttgtg ctttctaaag aagattttga
     1561 gaagaaggag aaaaataaag aggcaatata tagtggatat attagaaaca gaaagccctc
     1621 tgattctgtt cacattacaa atgatgatga aagatttcta catcatctta tcatagagga
     1681 gaaggaaaaa gatagcttta ctgctgtggt tatcacaggg gtacaaccag aacacataca
     1741 gtacttgaaa aattatttcc acctttggac acgacagtta gcgcatattt atcactacta
     1801 tattcatggc ccaaaaggaa atgaaatacg aacatcaaaa gaagttgaac ctttcaacaa
     1861 tattgatatt gaaatttcta tgtttgaaaa agggaaggta cctaagattg tcaacctaag
     1921 ggaaatacaa gacgacatgc agacgttgta tgtaaacaca gcagctgata gttttgaatt
     1981 caaagctcat gttgaaggag atggtgtagt ggaagggatt atccgttatc atccattctt
     2041 atatgataga gaaacttacc ctgatgatcc atgctttcca tcaaaattaa aagatgaaga
     2101 tgatgaagat gattgtttca tacttgagaa agcagctaga gggaaaaggc ctatttttga
     2161 atgtttttgg aatggacgat taataccata tacatcagtt gaagactttg actggtgtac
     2221 tcctcctaag aagagagggc ttgcaccaat tgaatgctac aataggatat ctggtgcatt
     2281 attcactaat gacaaattcc aggtcagcac aaataaattg acgtttatgg atcttgagct
     2341 aaaattgaaa gataagaaca ccctttttac aaggatttta aatggacagg aacagcgaat
     2401 gaaaattgac agagaatttg ctttgtggct gaaggactgt catgagaagt atgataaaca
     2461 aataaaattt acacttttta agggagtaat tacacgtcct gatcttcctt ctaaaaagca
     2521 aggtccctgg gcaacatatg cagcaataga atgggatgga aagatataca aagcaggaca
     2581 gctggtcaag acaatcaaga cacttcccct cttttatgga agcatagtaa gattttttct
     2641 ttatggcgat catgatggag aagtatatgc tacaggagga gaggttcaaa ttgcaatgga
     2701 acctcaggca ctatatgatg aagtaagaac tgtgccaatt gcaaagctgg ataggacagt
     2761 tgctgagaaa gctgttaaaa aatatgtaga agatgaaatg gcaaggctcc ctgatagatt
     2821 gtcagtaact tggcctgaag gagatgaatt attgcctaat gaggttaggc ctgctggaac
     2881 ccctattggt gcgttaagaa ttgaaatact gaataaaaaa ggggaagcaa tgcaaaagct
     2941 tccaggaaca agccatggag ggtcaaagaa actcctggtt gagctcaaag ttattttaca
     3001 ttcttcaagt ggaaataaag agattatttc gcatattagt caacatggag gaaaatggcc
     3061 ttactggttt aaaaaaatgg aaaatattca gaagttgggg aattatacct tgaaattaca
     3121 agttgtgttg aatgaaagta atgcagacac ttatgcagga agaccactac catctaaagc
     3181 aattaagttt tctgttaaag agggtaagcc agagaaattt tcatttggtc ttctggatct
     3241 tccttttcgt gttggagttc catttaatat ccctctggag tttcaggatg aatttggtca
     3301 taccagtcaa ctagtaactg atattcagcc agttcttgaa gcaagtggtt tatctttaca
     3361 ttatgaagaa ataaccaaag gaccaaattg tgtaattcga ggtgttacag ccaagggccc
     3421 tgtaaactct tgtcaaggca agaattataa tctgaaggtt actctgcctg gcttaaaaga
     3481 agactcacag attttgaaaa ttagattact acctggtcac cctcgtcgac tgaaagtgaa
     3541 acctgattct gaaattttag ttatagaaaa tggaacagct ttcccatttc aggtggaagt
     3601 tttagatgaa tcagacaaca taacagcaca accaaaattg attgttcatt gtaagttttc
     3661 aggtgctcca aaccttccag tctatgttgt agattgcagt agttctggaa ccagtatttt
     3721 aacaggatct gcaattcaag ttcagaatat taaaaaagac cagacgctta aagcaagaat
     3781 tgaaatacct agttgtaaag atgtggcacc tgtggagaag actattaagt tgcttcccag
     3841 tagccatgtt gcaagactac aaatattcag tgtagaagga caaaaggcaa ttcagatcaa
     3901 acatcaggat gaggttaatt ggatagcggg tgatattatg cataatctta tttttcaaat
     3961 gtatgatgaa ggagaaagag aaatcaatat aacatcagct ttagcagaaa aaattaaagt
     4021 taattggact cctgagatta acaaagaaca cttgctacag ggtctgcttc ctgatgtgca
     4081 agtaccaaca tctgtaaaag atatgcgcta ttgccaggtt tcattccaag atgatcatgt
     4141 gtctttggaa agtgcgttta cagtaagacc acttcctgat gaacctaaac atttaaaatg
     4201 tgaaatgaaa ggaggaaaaa cagtacagat gggccaagag cttcaaggag aagtagttat
     4261 aataattaca gatcagtacg gaaatcagat tcaagcattt tcaccaagtt ctttatcttc
     4321 tttgtcaatt gctggggttg gacttgatag ctcaaatttg aaaacaacct ttcaggaaaa
     4381 cacacagagt ataagtgtaa gaggcatcaa atttattcca ggtcctcctg gaaataagga
     4441 tctttgtttt acttggcgtg agttttctga ctttattcga gtgcaactaa tttctggacc
     4501 tcctgctaaa cttctcctta tagactggcc agaactaaag gagtccattc cagtgattaa
     4561 tggaagagat ttacagaacc ctattattgt tcaactttgt gatcagtggg ataatccagc
     4621 accggtacaa catgttaaaa taagtcttac aaaagctagc aatttaaagc tcatgccttc
     4681 aaaccaacag cataaaacag atgagaaagg cagggctaat ttgggagtat tcagtgtttt
     4741 tgcccctagg ggagagcata ctcttcaggt taaagccatc tataacaaaa gtatcataga
     4801 aggacctata attaagttaa tgattcttcc agacccagaa aaacccgttc gtctcaatgt
     4861 taaatatgac aaagatgcat ccttcttagc agggggtctt ttcactgatt ttatgattag
     4921 tgttatttct gaagatgaca gtatcattaa aaacattaat ccagcacgta tttccatgaa
     4981 aatgtggaag ctgtctacca gtgggaaccg acccccagca aatgcagaaa catttagttg
     5041 taataaaata aaagataatg acaaagaaga tggctgcttc tatttcaggg ataaagtaat
     5101 tcctaataaa gtggggacat attgtatcca gtttggtttt atgatggata aaacaaatat
     5161 tctcaacagt gaacaggtta tagttgaagt cctgcctaat caacctgtga agttagtacc
     5221 taaaattaaa ccacctacac cagctgtttc aaatgttcgc tcagttgcca gtaggacctt
     5281 ggtcagagat ctacatctta gtatcacgga tgactacgac aaccatactg gaattgattt
     5341 ggttggcact ataatagcca ccattaaagg ctctaatgag gaagatactg ataccccact
     5401 ttttattggg aaagttagaa cacttgaatt ccccttcgtg aatggttcgg ctgaaatcat
     5461 gagtctggtg ctggcagaaa gtagtcctgg aagggatagt actgaatatt ttattgtatt
     5521 tgagccccgg ctaccacttt tatcaagaac cttagaacca tatatcctac cgttcatgtt
     5581 ttacaatgat gttaagaagc agcaacaaat ggcagcactt acaaaagaaa aggaccaatt
     5641 atctcagtct attgttatgt ataaaagttt atttgaagcc agccaacagc ttcttaatga
     5701 aatgaaatgt caagttgaag aagcaagatt aaaagaggcc caattgcgaa atgaactaaa
     5761 aatacataat attgacattc ctacaacaca acaggtgcca cacattgaag cacttctgaa
     5821 aagaaagcta tcagaacaag aagaactgaa gaaaaaacct agaagatcgt gtactcttcc
     5881 aaactatact aaaggcagtg gagatgtttt gggaaagatt gcacatctag cacaaattga
     5941 agatgataga gctgcgatgg ttatttcttg gcatctggca agtgacatgg actgtgtagt
     6001 caccctaacc actgacgctg cacgtcgtat ctatgatgaa acccaaggtc gtcagcaggt
     6061 gttgcccctt gattctattt acaagaagac tcttccagat tggaaaagat ctctacctca
     6121 tttccgaaat ggaaaattgt attttaaacc cattggagat ccagtctttg ctcgagactt
     6181 gttaacattt ccagataatg tagaacattg tgaaacagta tttggtatgc tgttaggaga
     6241 caccattatt ttggataatc tggatgcggc caatcattat agaaaagagg ttgttaaaat
     6301 tacacactgt cctacactgc tgaccagaga tggagatcga attgactaca aagaccatga
     6361 cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaagt gagtcgacaa
     6421 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6481 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6541 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6601 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6661 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6721 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6781 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     6841 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6901 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6961 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattctacc
     7021 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     7081 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     7141 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     7201 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     7261 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     7321 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     7381 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     7441 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     7501 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
     7561 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     7621 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     7681 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     7741 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     7801 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     7861 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     7921 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     7981 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     8041 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     8101 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     8161 gcgcccgacc gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac
     8221 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8281 aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     8341 ggttagacca gatctgagcc tgggagctct ctggctgcct agggaaccca ctgcttaagc
     8401 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8461 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     8521 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8581 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8701 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
     8761 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8821 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgcg tcgagacgta
     8881 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     8941 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     9001 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     9061 ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9121 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9181 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9241 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9301 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9361 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9421 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9481 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccag
     9541 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9601 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9661 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9721 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9781 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9841 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9901 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9961 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10021 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10081 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10141 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10201 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10261 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10321 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10381 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10441 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10501 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10561 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10621 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10681 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10741 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10801 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    10861 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10921 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10981 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11041 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11101 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11161 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11221 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11281 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11341 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11401 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11461 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11521 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11581 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11641 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    11701 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
    11761 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
    11821 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
    11881 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
    11941 ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgggtct ctctggttag
    12001 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    12061 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    12121 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
    12181 ggacctgaaa gcgaaaggga aaccagagct ctctcgacgc aggactcggc ttgctgaagc
    12241 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
    12301 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
    12361 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
    12421 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
    12481 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
    12541 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
    12601 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
    12661 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
    12721 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
    12781 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
    12841 ttgggttctt gggagcagca ggaagcacta tgggcgcagc ctcaatgacg ctgacggtac
    12901 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
    12961 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
    13021 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
    13081 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
    13141 aacagattgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    13201 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    13261 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    13321 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    13381 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    13441 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
    13501 agacagagac agatccattc gattagtgaa cggatc
//
