LOCUS       Exported               12636 bp ds-DNA     circular SYN 06-JUL-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Yosuke
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 16, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        153..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        457..660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             743..5512
                     /codon_start=1
                     /label=Ex1-36.47M-48.3Flag
                     /translation="MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPL
                     QVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVN
                     QLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVR
                     RIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPV
                     PVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEA
                     IYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFH
                     LWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDM
                     QTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDC
                     FILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTND
                     KFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKF
                     TLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYG
                     DHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLS
                     VTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILH
                     SSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSK
                     AIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLS
                     LHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRL
                     KVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSG
                     TSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQK
                     AIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQG
                     LLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQE
                     LQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFI
                     PGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQ
                     LCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQV
                     KAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSII
                     KNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYC
                     IQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLS
                     ITMTPIRKCNDSLRHSPKVETTDCPVPPKRMRREATRQNRIITKTDVDYKDHDGDYKDH
                     DIDYKDDDDK"
     exon            743..5308
                     /label=Ex1-36
     primer_bind     4652..4681
                     /label=S34
     primer_bind     5289..5328
                     /label=S145
     primer_bind     complement(5289..5328)
                     /label=S144
     exon            5309..5443
                     /label=Ex47M-48
     primer_bind     complement(5423..5521)
                     /label=S82
     CDS             5444..5509
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5519..6107
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5990..6001)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6121..6620
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             6641..7240
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             7378..7611
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7683..7804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7823..7958
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7991..8009)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8019..8035)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8180..8635
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8664..8768
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8769..9629
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9800..10388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    10676..10697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10712..10742
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10750..10766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10774..10790
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10811..10829
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             11085..11265
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11309..11434
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11927..12160
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tcgacggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
       61 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      121 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      661 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      721 agacaccgac tctagaggat ccatggcagc ggcggacggc ggcgggcctg gtggggcctc
      781 tgtggggact gaggaggatg gcggaggcgt cggccacagg acggtgtact tgtttgatcg
      841 gcgcgaaaag gagtccgagc tcggggaccg gcctctgcag gtcggggagc gctcggacta
      901 cgcgggattt cgcgcgtgtg tgtgtcagac acttggcatt tcacctgaag aaaaatttgt
      961 tattacaaca acaagtagga aagaaattac ctgtgataat tttgatgaaa ctgttaaaga
     1021 tggagtcacc ttatacctgc tacagtcggt caatcagtta ctactgacag ctacgaaaga
     1081 acgaattgac ttcttacctc actatgacac actggttaaa agtggcatgt atgaatatta
     1141 tgccagtgaa ggacaaaatc ctttgccatt tgctcttgcg gaattaattg acaattcatt
     1201 gtctgctact tctcgtaaca ttggggttag aagaatacag atcaaattgc tttttgatga
     1261 aacacaagga aaacctgctg ttgcagtgat agataatgga agaggaatga cctctaaaca
     1321 gcttaacaac tgggccgtgt ataggttgtc aaaattcaca aggcaaggtg actttgaaag
     1381 tgatcattca ggatatgttc gtccagtacc agtgccacgc agtttaaata gtgatatttc
     1441 ctattttggt gttgggggca agcaagctgt cttctttgtt ggacaatcag ccagaatgat
     1501 aagcaaacct gcagattccc aagatgttca cgagcttgtg ctttctaaag aagattttga
     1561 gaagaaggag aaaaataaag aggcaatata tagtggatat attagaaaca gaaagccctc
     1621 tgattctgtt cacattacaa atgatgatga aagatttcta catcatctta tcatagagga
     1681 gaaggaaaaa gatagcttta ctgctgtggt tatcacaggg gtacaaccag aacacataca
     1741 gtacttgaaa aattatttcc acctttggac acgacagtta gcgcatattt atcactacta
     1801 tattcatggc ccaaaaggaa atgaaatacg aacatcaaaa gaagttgaac ctttcaacaa
     1861 tattgatatt gaaatttcta tgtttgaaaa agggaaggta cctaagattg tcaacctaag
     1921 ggaaatacaa gacgacatgc agacgttgta tgtaaacaca gcagctgata gttttgaatt
     1981 caaagctcat gttgaaggag atggtgtagt ggaagggatt atccgttatc atccattctt
     2041 atatgataga gaaacttacc ctgatgatcc atgctttcca tcaaaattaa aagatgaaga
     2101 tgatgaagat gattgtttca tacttgagaa agcagctaga gggaaaaggc ctatttttga
     2161 atgtttttgg aatggacgat taataccata tacatcagtt gaagactttg actggtgtac
     2221 tcctcctaag aagagagggc ttgcaccaat tgaatgctac aataggatat ctggtgcatt
     2281 attcactaat gacaaattcc aggtcagcac aaataaattg acgtttatgg atcttgagct
     2341 aaaattgaaa gataagaaca ccctttttac aaggatttta aatggacagg aacagcgaat
     2401 gaaaattgac agagaatttg ctttgtggct gaaggactgt catgagaagt atgataaaca
     2461 aataaaattt acacttttta agggagtaat tacacgtcct gatcttcctt ctaaaaagca
     2521 aggtccctgg gcaacatatg cagcaataga atgggatgga aagatataca aagcaggaca
     2581 gctggtcaag acaatcaaga cacttcccct cttttatgga agcatagtaa gattttttct
     2641 ttatggcgat catgatggag aagtatatgc tacaggagga gaggttcaaa ttgcaatgga
     2701 acctcaggca ctatatgatg aagtaagaac tgtgccaatt gcaaagctgg ataggacagt
     2761 tgctgagaaa gctgttaaaa aatatgtaga agatgaaatg gcaaggctcc ctgatagatt
     2821 gtcagtaact tggcctgaag gagatgaatt attgcctaat gaggttaggc ctgctggaac
     2881 ccctattggt gcgttaagaa ttgaaatact gaataaaaaa ggggaagcaa tgcaaaagct
     2941 tccaggaaca agccatggag ggtcaaagaa actcctggtt gagctcaaag ttattttaca
     3001 ttcttcaagt ggaaataaag agattatttc gcatattagt caacatggag gaaaatggcc
     3061 ttactggttt aaaaaaatgg aaaatattca gaagttgggg aattatacct tgaaattaca
     3121 agttgtgttg aatgaaagta atgcagacac ttatgcagga agaccactac catctaaagc
     3181 aattaagttt tctgttaaag agggtaagcc agagaaattt tcatttggtc ttctggatct
     3241 tccttttcgt gttggagttc catttaatat ccctctggag tttcaggatg aatttggtca
     3301 taccagtcaa ctagtaactg atattcagcc agttcttgaa gcaagtggtt tatctttaca
     3361 ttatgaagaa ataaccaaag gaccaaattg tgtaattcga ggtgttacag ccaagggccc
     3421 tgtaaactct tgtcaaggca agaattataa tctgaaggtt actctgcctg gcttaaaaga
     3481 agactcacag attttgaaaa ttagattact acctggtcac cctcgtcgac tgaaagtgaa
     3541 acctgattct gaaattttag ttatagaaaa tggaacagct ttcccatttc aggtggaagt
     3601 tttagatgaa tcagacaaca taacagcaca accaaaattg attgttcatt gtaagttttc
     3661 aggtgctcca aaccttccag tctatgttgt agattgcagt agttctggaa ccagtatttt
     3721 aacaggatct gcaattcaag ttcagaatat taaaaaagac cagacgctta aagcaagaat
     3781 tgaaatacct agttgtaaag atgtggcacc tgtggagaag actattaagt tgcttcccag
     3841 tagccatgtt gcaagactac aaatattcag tgtagaagga caaaaggcaa ttcagatcaa
     3901 acatcaggat gaggttaatt ggatagcggg tgatattatg cataatctta tttttcaaat
     3961 gtatgatgaa ggagaaagag aaatcaatat aacatcagct ttagcagaaa aaattaaagt
     4021 taattggact cctgagatta acaaagaaca cttgctacag ggtctgcttc ctgatgtgca
     4081 agtaccaaca tctgtaaaag atatgcgcta ttgccaggtt tcattccaag atgatcatgt
     4141 gtctttggaa agtgcgttta cagtaagacc acttcctgat gaacctaaac atttaaaatg
     4201 tgaaatgaaa ggaggaaaaa cagtacagat gggccaagag cttcaaggag aagtagttat
     4261 aataattaca gatcagtacg gaaatcagat tcaagcattt tcaccaagtt ctttatcttc
     4321 tttgtcaatt gctggggttg gacttgatag ctcaaatttg aaaacaacct ttcaggaaaa
     4381 cacacagagt ataagtgtaa gaggcatcaa atttattcca ggtcctcctg gaaataagga
     4441 tctttgtttt acttggcgtg agttttctga ctttattcga gtgcaactaa tttctggacc
     4501 tcctgctaaa cttctcctta tagactggcc agaactaaag gagtccattc cagtgattaa
     4561 tggaagagat ttacagaacc ctattattgt tcaactttgt gatcagtggg ataatccagc
     4621 accggtacaa catgttaaaa taagtcttac aaaagctagc aatttaaagc tcatgccttc
     4681 aaaccaacag cataaaacag atgagaaagg cagggctaat ttgggagtat tcagtgtttt
     4741 tgcccctagg ggagagcata ctcttcaggt taaagccatc tataacaaaa gtatcataga
     4801 aggacctata attaagttaa tgattcttcc agacccagaa aaacccgttc gtctcaatgt
     4861 taaatatgac aaagatgcat ccttcttagc agggggtctt ttcactgatt ttatgattag
     4921 tgttatttct gaagatgaca gtatcattaa aaacattaat ccagcacgta tttccatgaa
     4981 aatgtggaag ctgtctacca gtgggaaccg acccccagca aatgcagaaa catttagttg
     5041 taataaaata aaagataatg acaaagaaga tggctgcttc tatttcaggg ataaagtaat
     5101 tcctaataaa gtggggacat attgtatcca gtttggtttt atgatggata aaacaaatat
     5161 tctcaacagt gaacaggtta tagttgaagt cctgcctaat caacctgtga agttagtacc
     5221 taaaattaaa ccacctacac cagctgtttc aaatgttcgc tcagttgcca gtaggacctt
     5281 ggtcagagat ctacatctta gtatcacgat gactcccata cgtaagtgta atgactcatt
     5341 gcgtcattca ccaaaggttg agacgacaga ttgtccagtt cctcctaaaa gaatgagacg
     5401 agaagctaca agacaaaata ggattataac caaaacagat gtagactaca aagaccatga
     5461 cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaagt gagtcgacaa
     5521 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5581 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5641 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5701 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5761 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5821 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5881 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     5941 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6001 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6061 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattctacc
     6121 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     6181 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     6241 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     6301 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     6361 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     6421 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     6481 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     6541 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     6601 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
     6661 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     6721 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     6781 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6841 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6901 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6961 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     7021 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     7081 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     7141 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     7201 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     7261 gcgcccgacc gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac
     7321 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7381 aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     7441 ggttagacca gatctgagcc tgggagctct ctggctgcct agggaaccca ctgcttaagc
     7501 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7561 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7621 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7681 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7741 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7801 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
     7861 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7921 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgcg tcgagacgta
     7981 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     8041 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8101 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8161 ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8221 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8281 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8341 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     8401 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8461 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8521 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8581 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccag
     8641 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8701 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8761 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8821 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8881 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8941 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9001 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9061 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9121 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9181 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9241 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9301 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9361 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9421 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9481 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9541 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9601 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9661 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9721 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9781 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9841 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9901 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     9961 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10021 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10081 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10141 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10201 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10261 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10321 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10381 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10441 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10501 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10561 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10621 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    10681 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    10741 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    10801 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
    10861 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
    10921 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
    10981 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
    11041 ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgggtct ctctggttag
    11101 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    11161 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    11221 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
    11281 ggacctgaaa gcgaaaggga aaccagagct ctctcgacgc aggactcggc ttgctgaagc
    11341 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
    11401 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
    11461 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
    11521 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
    11581 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
    11641 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
    11701 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
    11761 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
    11821 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
    11881 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
    11941 ttgggttctt gggagcagca ggaagcacta tgggcgcagc ctcaatgacg ctgacggtac
    12001 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
    12061 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
    12121 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
    12181 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
    12241 aacagattgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    12301 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    12361 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    12421 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    12481 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    12541 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
    12601 agacagagac agatccattc gattagtgaa cggatc
//
