LOCUS       Exported                9894 bp ds-DNA     circular SYN 05-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Yosuke
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 16, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        153..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        457..660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             743..2770
                     /codon_start=1
                     /label=Ex1-10A.11D-14.47M-48.3Flag
                     /translation="MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPL
                     QVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVN
                     QLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVR
                     RIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPV
                     PVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEA
                     IYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFH
                     LWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDM
                     QTLYVNTAADDEDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAP
                     IECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFAL
                     WLKDCHEKYDKQIKFTLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKT
                     LPLFYGSIVRFFLYGDHDGEVYATGGEVQIAMMTPIRKCNDSLRHSPKVETTDCPVPPK
                     RMRREATRQNRIITKTDVDYKDHDGDYKDHDIDYKDDDDK"
     exon            743..1966
                     /label=Ex1-10A
     primer_bind     1947..1986
                     /label=S154
     primer_bind     complement(1947..1986)
                     /label=S153
     exon            1967..2701
                     /label=Ex11D-14.47M-48
     misc_feature    1967..2566
                     /label=Ex11D-14
     misc_feature    1967..1990
                     /label=Thylakoidal
     misc_feature    2024..2047
                     /label=Cathepsin G
     misc_feature    2051..2074
                     /label=MMP9
     misc_feature    2567..2701
                     /label=Ex47M-48
     primer_bind     complement(2681..2779)
                     /label=S82
     CDS             2702..2767
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2777..3365
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3248..3259)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3379..3878
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3899..4498
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             4636..4869
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4941..5062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5081..5216
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5249..5267)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5277..5293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5438..5893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5922..6026
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6027..6887
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7058..7646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7934..7955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7970..8000
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8008..8024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8032..8048
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8069..8087
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             8343..8523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8567..8692
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9185..9418
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tcgacggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
       61 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      121 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      661 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      721 agacaccgac tctagaggat ccatggcagc ggcggacggc ggcgggcctg gtggggcctc
      781 tgtggggact gaggaggatg gcggaggcgt cggccacagg acggtgtact tgtttgatcg
      841 gcgcgaaaag gagtccgagc tcggggaccg gcctctgcag gtcggggagc gctcggacta
      901 cgcgggattt cgcgcgtgtg tgtgtcagac acttggcatt tcacctgaag aaaaatttgt
      961 tattacaaca acaagtagga aagaaattac ctgtgataat tttgatgaaa ctgttaaaga
     1021 tggagtcacc ttatacctgc tacagtcggt caatcagtta ctactgacag ctacgaaaga
     1081 acgaattgac ttcttacctc actatgacac actggttaaa agtggcatgt atgaatatta
     1141 tgccagtgaa ggacaaaatc ctttgccatt tgctcttgcg gaattaattg acaattcatt
     1201 gtctgctact tctcgtaaca ttggggttag aagaatacag atcaaattgc tttttgatga
     1261 aacacaagga aaacctgctg ttgcagtgat agataatgga agaggaatga cctctaaaca
     1321 gcttaacaac tgggccgtgt ataggttgtc aaaattcaca aggcaaggtg actttgaaag
     1381 tgatcattca ggatatgttc gtccagtacc agtgccacgc agtttaaata gtgatatttc
     1441 ctattttggt gttgggggca agcaagctgt cttctttgtt ggacaatcag ccagaatgat
     1501 aagcaaacct gcagattccc aagatgttca cgagcttgtg ctttctaaag aagattttga
     1561 gaagaaggag aaaaataaag aggcaatata tagtggatat attagaaaca gaaagccctc
     1621 tgattctgtt cacattacaa atgatgatga aagatttcta catcatctta tcatagagga
     1681 gaaggaaaaa gatagcttta ctgctgtggt tatcacaggg gtacaaccag aacacataca
     1741 gtacttgaaa aattatttcc acctttggac acgacagtta gcgcatattt atcactacta
     1801 tattcatggc ccaaaaggaa atgaaatacg aacatcaaaa gaagttgaac ctttcaacaa
     1861 tattgatatt gaaatttcta tgtttgaaaa agggaaggta cctaagattg tcaacctaag
     1921 ggaaatacaa gacgacatgc agacgttgta tgtaaacaca gcagctgatg atgaagatga
     1981 ttgtttcata cttgagaaag cagctagagg gaaaaggcct atttttgaat gtttttggaa
     2041 tggacgatta ataccatata catcagttga agactttgac tggtgtactc ctcctaagaa
     2101 gagagggctt gcaccaattg aatgctacaa taggatatct ggtgcattat tcactaatga
     2161 caaattccag gtcagcacaa ataaattgac gtttatggat cttgagctaa aattgaaaga
     2221 taagaacacc ctttttacaa ggattttaaa tggacaggaa cagcgaatga aaattgacag
     2281 agaatttgct ttgtggctga aggactgtca tgagaagtat gataaacaaa taaaatttac
     2341 actttttaag ggagtaatta cacgtcctga tcttccttct aaaaagcaag gtccctgggc
     2401 aacatatgca gcaatagaat gggatggaaa gatatacaaa gcaggacagc tggtcaagac
     2461 aatcaagaca cttcccctct tttatggaag catagtaaga ttttttcttt atggcgatca
     2521 tgatggagaa gtatatgcta caggaggaga ggttcaaatt gcaatgatga ctcccatacg
     2581 taagtgtaat gactcattgc gtcattcacc aaaggttgag acgacagatt gtccagttcc
     2641 tcctaaaaga atgagacgag aagctacaag acaaaatagg attataacca aaacagatgt
     2701 agactacaaa gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga
     2761 tgacaagtga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2821 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2881 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2941 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3001 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3061 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3121 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     3181 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3241 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3301 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3361 cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     3421 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     3481 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     3541 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     3601 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     3661 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     3721 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     3781 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     3841 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     3901 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3961 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4021 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4081 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4141 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4201 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4261 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4321 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4381 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4441 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     4501 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg
     4561 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4621 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg
     4681 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctgcctag
     4741 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4801 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4861 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4921 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     4981 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5041 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     5101 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5161 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5221 cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     5281 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5341 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5401 tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta
     5461 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5521 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5581 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5641 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5701 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5761 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5821 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5881 acgtttacaa tttcccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5941 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6001 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6061 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6121 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6181 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6241 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6301 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6361 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6421 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6481 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6541 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6601 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6661 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6721 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6781 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6841 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6901 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6961 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7021 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7081 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7141 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7201 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7261 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7321 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7381 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7441 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7501 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7561 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7621 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7681 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7741 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7801 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7861 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7921 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7981 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8041 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     8101 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     8161 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     8221 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     8281 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa
     8341 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8401 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8461 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8521 gcagtggcgc ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag
     8581 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     8641 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     8701 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     8761 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     8821 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     8881 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     8941 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     9001 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     9061 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     9121 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     9181 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct
     9241 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     9301 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     9361 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     9421 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     9481 ggagtaataa atctctggaa cagattggaa tcacacgacc tggatggagt gggacagaga
     9541 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     9601 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     9661 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     9721 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     9781 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     9841 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatc
//
