LOCUS       Exported                9915 bp ds-DNA     circular SYN 17-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Yosuke
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 16, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        153..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        457..660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             743..2791
                     /codon_start=1
                     /label=Ex1-11E.12D-14.47M-48.3Flag
                     /translation="MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPL
                     QVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVN
                     QLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVR
                     RIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPV
                     PVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEA
                     IYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFH
                     LWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDM
                     QTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDWCTPP
                     KKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMK
                     IDREFALWLKDCHEKYDKQIKFTLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQ
                     LVKTIKTLPLFYGSIVRFFLYGDHDGEVYATGGEVQIAMMTPIRKCNDSLRHSPKVETT
                     DCPVPPKRMRREATRQNRIITKTDVDYKDHDGDYKDHDIDYKDDDDK"
     exon            743..2098
                     /label=Ex1-11E
     primer_bind     2079..2118
                     /label=S162
     primer_bind     complement(2079..2118)
                     /label=S161
     exon            2099..2587
                     /label=Ex12D-14
     exon            2588..2722
                     /label=Ex47M-48
     primer_bind     complement(2702..2800)
                     /label=S82
     CDS             2723..2788
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2798..3386
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3269..3280)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3400..3899
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3920..4519
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             4657..4890
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4962..5083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5102..5237
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5270..5288)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5298..5314)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5459..5914
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5943..6047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6048..6908
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7079..7667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7955..7976
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7991..8021
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8029..8045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8053..8069
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8090..8108
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             8364..8544
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8588..8713
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9206..9439
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tcgacggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
       61 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      121 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      661 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      721 agacaccgac tctagaggat ccatggcagc ggcggacggc ggcgggcctg gtggggcctc
      781 tgtggggact gaggaggatg gcggaggcgt cggccacagg acggtgtact tgtttgatcg
      841 gcgcgaaaag gagtccgagc tcggggaccg gcctctgcag gtcggggagc gctcggacta
      901 cgcgggattt cgcgcgtgtg tgtgtcagac acttggcatt tcacctgaag aaaaatttgt
      961 tattacaaca acaagtagga aagaaattac ctgtgataat tttgatgaaa ctgttaaaga
     1021 tggagtcacc ttatacctgc tacagtcggt caatcagtta ctactgacag ctacgaaaga
     1081 acgaattgac ttcttacctc actatgacac actggttaaa agtggcatgt atgaatatta
     1141 tgccagtgaa ggacaaaatc ctttgccatt tgctcttgcg gaattaattg acaattcatt
     1201 gtctgctact tctcgtaaca ttggggttag aagaatacag atcaaattgc tttttgatga
     1261 aacacaagga aaacctgctg ttgcagtgat agataatgga agaggaatga cctctaaaca
     1321 gcttaacaac tgggccgtgt ataggttgtc aaaattcaca aggcaaggtg actttgaaag
     1381 tgatcattca ggatatgttc gtccagtacc agtgccacgc agtttaaata gtgatatttc
     1441 ctattttggt gttgggggca agcaagctgt cttctttgtt ggacaatcag ccagaatgat
     1501 aagcaaacct gcagattccc aagatgttca cgagcttgtg ctttctaaag aagattttga
     1561 gaagaaggag aaaaataaag aggcaatata tagtggatat attagaaaca gaaagccctc
     1621 tgattctgtt cacattacaa atgatgatga aagatttcta catcatctta tcatagagga
     1681 gaaggaaaaa gatagcttta ctgctgtggt tatcacaggg gtacaaccag aacacataca
     1741 gtacttgaaa aattatttcc acctttggac acgacagtta gcgcatattt atcactacta
     1801 tattcatggc ccaaaaggaa atgaaatacg aacatcaaaa gaagttgaac ctttcaacaa
     1861 tattgatatt gaaatttcta tgtttgaaaa agggaaggta cctaagattg tcaacctaag
     1921 ggaaatacaa gacgacatgc agacgttgta tgtaaacaca gcagctgata gttttgaatt
     1981 caaagctcat gttgaaggag atggtgtagt ggaagggatt atccgttatc atccattctt
     2041 atatgataga gaaacttacc ctgatgatcc atgctttcca tcaaaattaa aagatgaaga
     2101 ctggtgtact cctcctaaga agagagggct tgcaccaatt gaatgctaca ataggatatc
     2161 tggtgcatta ttcactaatg acaaattcca ggtcagcaca aataaattga cgtttatgga
     2221 tcttgagcta aaattgaaag ataagaacac cctttttaca aggattttaa atggacagga
     2281 acagcgaatg aaaattgaca gagaatttgc tttgtggctg aaggactgtc atgagaagta
     2341 tgataaacaa ataaaattta cactttttaa gggagtaatt acacgtcctg atcttccttc
     2401 taaaaagcaa ggtccctggg caacatatgc agcaatagaa tgggatggaa agatatacaa
     2461 agcaggacag ctggtcaaga caatcaagac acttcccctc ttttatggaa gcatagtaag
     2521 attttttctt tatggcgatc atgatggaga agtatatgct acaggaggag aggttcaaat
     2581 tgcaatgatg actcccatac gtaagtgtaa tgactcattg cgtcattcac caaaggttga
     2641 gacgacagat tgtccagttc ctcctaaaag aatgagacga gaagctacaa gacaaaatag
     2701 gattataacc aaaacagatg tagactacaa agaccatgac ggtgattata aagatcatga
     2761 catcgattac aaggatgacg atgacaagtg agtcgacaat caacctctgg attacaaaat
     2821 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     2881 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     2941 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3001 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3061 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3121 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3181 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     3241 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3301 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3361 gatctccctt tgggccgcct ccccgcctgg aattctaccg ggtaggggag gcgcttttcc
     3421 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     3481 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     3541 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     3601 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     3661 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     3721 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     3781 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     3841 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     3901 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3961 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4021 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4081 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4141 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4201 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4261 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4321 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4381 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4441 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4501 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     4561 acgaccccat gcatctcgag ggcccggtac ctttaagacc aatgacttac aaggcagctg
     4621 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac
     4681 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     4741 gggagctctc tggctgccta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     4801 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     4861 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     4921 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     4981 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5041 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     5101 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5161 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5221 aggctttttt ggaggcctag gcttttgcgt cgagacgtac ccaattcgcc ctatagtgag
     5281 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     5341 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     5401 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcgac
     5461 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5521 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5581 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5641 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5701 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5761 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5821 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5881 gcgaatttta acaaaatatt aacgtttaca atttcccagg tggcactttt cggggaaatg
     5941 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6001 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6061 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6121 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6181 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6241 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6301 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6361 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6421 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6481 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6541 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6601 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6661 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6721 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6781 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6841 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6901 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6961 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7021 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7081 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7141 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7201 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     7261 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7321 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7381 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7441 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7501 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7561 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7621 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7681 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7741 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7801 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7861 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7921 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7981 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8041 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     8101 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     8161 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     8221 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     8281 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     8341 gtgcctagct cgatacaata aacgggtctc tctggttaga ccagatctga gcctgggagc
     8401 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8461 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8521 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacctgaaag cgaaagggaa
     8581 accagagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     8641 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     8701 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     8761 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     8821 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     8881 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8941 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     9001 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     9061 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     9121 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     9181 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     9241 gaagcactat gggcgcagcc tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9301 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9361 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9421 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9481 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagattgga atcacacgac
     9541 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     9601 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     9661 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     9721 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     9781 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     9841 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     9901 attagtgaac ggatc
//
