LOCUS       Exported                9387 bp ds-DNA     circular SYN 25-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Yosuke
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 16, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        153..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        457..660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     734..763
                     /label=S131
     CDS             743..2263
                     /codon_start=1
                     /label=Ex37-48.3Flag
                     /translation="MDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFV
                     NGSAEIMSLVLAESSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAA
                     LTKEKDQLSQSIVMYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQ
                     VPHIEALLKRKLSEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISW
                     HLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFK
                     PIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLT
                     RDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLD
                     SVNKDLNSQLEYLRTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVET
                     TDCPVPPKRMRREATRQNRIITKTDVDYKDHDGDYKDHDIDYKDDDDK"
     CDS             746..2194
                     /codon_start=1
                     /label=Ex37-48
                     /translation="DDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVN
                     GSAEIMSLVLAESSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAAL
                     TKEKDQLSQSIVMYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQV
                     PHIEALLKRKLSEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWH
                     LASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKP
                     IGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTR
                     DGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDS
                     VNKDLNSQLEYLRTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVETT
                     DCPVPPKRMRREATRQNRIITKTDV"
     primer_bind     complement(2174..2272)
                     /label=S82
     CDS             2195..2260
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2270..2858
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2741..2752)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2872..3371
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3392..3991
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             4129..4362
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4434..4555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4574..4709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4742..4760)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4770..4786)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4931..5386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5415..5519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5520..6380
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6551..7139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7427..7448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7463..7493
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7501..7517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7525..7541
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7562..7580
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             7836..8016
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8060..8185
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8678..8911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tcgacggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
       61 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      121 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      661 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      721 agacaccgac tctagaggat ccatggatga ctacgacaac catactggaa ttgatttggt
      781 tggcactata atagccacca ttaaaggctc taatgaggaa gatactgata ccccactttt
      841 tattgggaaa gttagaacac ttgaattccc cttcgtgaat ggttcggctg aaatcatgag
      901 tctggtgctg gcagaaagta gtcctggaag ggatagtact gaatatttta ttgtatttga
      961 gccccggcta ccacttttat caagaacctt agaaccatat atcctaccgt tcatgtttta
     1021 caatgatgtt aagaagcagc aacaaatggc agcacttaca aaagaaaagg accaattatc
     1081 tcagtctatt gttatgtata aaagtttatt tgaagccagc caacagcttc ttaatgaaat
     1141 gaaatgtcaa gttgaagaag caagattaaa agaggcccaa ttgcgaaatg aactaaaaat
     1201 acataatatt gacattccta caacacaaca ggtgccacac attgaagcac ttctgaaaag
     1261 aaagctatca gaacaagaag aactgaagaa aaaacctaga agatcgtgta ctcttccaaa
     1321 ctatactaaa ggcagtggag atgttttggg aaagattgca catctagcac aaattgaaga
     1381 tgatagagct gcgatggtta tttcttggca tctggcaagt gacatggact gtgtagtcac
     1441 cctaaccact gacgctgcac gtcgtatcta tgatgaaacc caaggtcgtc agcaggtgtt
     1501 gccccttgat tctatttaca agaagactct tccagattgg aaaagatctc tacctcattt
     1561 ccgaaatgga aaattgtatt ttaaacccat tggagatcca gtctttgctc gagacttgtt
     1621 aacatttcca gataatgtag aacattgtga aacagtattt ggtatgctgt taggagacac
     1681 cattattttg gataatctgg atgcggccaa tcattataga aaagaggttg ttaaaattac
     1741 acactgtcct acactgctga ccagagatgg agatcgaatt cgaagtaatg gaaagtttgg
     1801 gggccttcag aataaagctc ctccaatgga taaacttcgg ggaatggtat ttggagctcc
     1861 agttccaaaa cagtgtctga tcttagggga acaaatagat cttcttcagc agtatcgttc
     1921 tgctgtgtgc aaactagaca gtgtgaataa ggatcttaac agtcaattag agtaccttcg
     1981 cactccggat atgaggaaga aaaagcaaga acttgatgaa catgagaaaa atctcaaact
     2041 aatagaggaa aaactaggta tgactcccat acgtaagtgt aatgactcat tgcgtcattc
     2101 accaaaggtt gagacgacag attgtccagt tcctcctaaa agaatgagac gagaagctac
     2161 aagacaaaat aggattataa ccaaaacaga tgtagactac aaagaccatg acggtgatta
     2221 taaagatcat gacatcgatt acaaggatga cgatgacaag tgagtcgaca atcaacctct
     2281 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     2341 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     2401 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     2461 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2521 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     2581 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     2641 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     2701 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     2761 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     2821 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac cgggtagggg
     2881 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     2941 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     3001 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     3061 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     3121 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     3181 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     3241 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     3301 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     3361 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     3421 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     3481 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     3541 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     3601 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     3661 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     3721 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     3781 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     3841 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     3901 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     3961 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     4021 cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga ccaatgactt
     4081 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag
     4141 ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     4201 agatctgagc ctgggagctc tctggctgcc tagggaaccc actgcttaag cctcaataaa
     4261 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4321 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     4381 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     4441 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4501 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4561 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4681 gaagtagtga ggaggctttt ttggaggcct aggcttttgc gtcgagacgt acccaattcg
     4741 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     4801 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4861 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4921 gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4981 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5041 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5101 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5161 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5221 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5281 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5341 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca ggtggcactt
     5401 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5461 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5521 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5581 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5641 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5701 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5761 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5821 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5881 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5941 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6001 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6061 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6121 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6181 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6241 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6301 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6361 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6421 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6481 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6541 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6601 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6661 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6721 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6781 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6841 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6901 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6961 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7021 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7081 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7141 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7201 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7261 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7321 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     7381 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     7441 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     7501 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     7561 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     7621 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     7681 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     7741 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     7801 tattgtattt aagtgcctag ctcgatacaa taaacgggtc tctctggtta gaccagatct
     7861 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7921 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7981 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacctgaa
     8041 agcgaaaggg aaaccagagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     8101 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     8161 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     8221 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     8281 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     8341 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     8401 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     8461 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     8521 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     8581 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     8641 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     8701 tgggagcagc aggaagcact atgggcgcag cctcaatgac gctgacggta caggccagac
     8761 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     8821 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     8881 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     8941 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattg
     9001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     9061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     9121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     9181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     9241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     9301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     9361 cagatccatt cgattagtga acggatc
//
