LOCUS       Exported                9086 bp ds-DNA     circular SYN 29-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Jul 29, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    263..826
                     /label=pU6
                     /label=pU6 (pMJ177) 5' CMV promoter IE
     misc_feature    complement(766..775)
                     /label=GC Box
     misc_feature    844..1024
                     /label=HIV-1_5_LTR
     misc_feature    complement(1599..1605)
                     /label=TATA box
     misc_feature    1693..1926
                     /label=RRE
     misc_feature    2457..2471
                     /label=cPPT
     misc_feature    2680..3641
                     /label=EF1Alpha
     misc_feature    3643..3797
                     /label=EF1Alpha
                     /label=EF1Alpha(1)
     misc_feature    3866..4522
                     /label=puro-t2A
     misc_feature    4082..4088
                     /label=RR47
     misc_feature    4083..4088
                     /label=RR48
     misc_feature    4460..4534
                     /label=T2A
     misc_feature    4535..5251
                     /label=GFP
     misc_feature    5252..5263
                     /label=linker
     misc_feature    5264..5866
                     /label=RAB10
     misc_recomb     5886..5919
                     /label=LoxP recombination site
                     /label=LoxP recombination site(1)
     misc_feature    5965..6565
                     /label=wPRE
     misc_feature    6755..6769
                     /label=cPPT
                     /label=cPPT(1)
     misc_feature    7092..7272
                     /label=HIV-1_5_LTR
                     /label=HIV-1_5_LTR(1)
     rep_origin      complement(7316..7998)
                     /direction=LEFT
                     /label=ColE1 origin
     misc_feature    complement(7319..7938)
                     /label=pBR322_origin
     misc_feature    complement(7343..7352)
                     /label=GC Box
                     /label=GC Box(1)
     CDS             complement(8096..8755)
                     /label=AmpR
     misc_feature    complement(8995..9023)
                     /label=AmpR promoter
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     1561 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1621 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1681 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1741 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1801 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1861 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1921 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1981 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     2041 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2101 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     2161 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     2221 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     2281 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2341 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2401 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2461 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2521 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2581 acagggacag cagagatcca gtttggttag taccgggccc gcttcgagta ctaggatcca
     2641 ttaggcggcc gcgtggataa ccgtattacc gccatgcatg tgcccgtcag tgggcagagc
     2701 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     2761 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     2821 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     2881 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     2941 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
     3001 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     3061 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     3121 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3181 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3241 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3301 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3361 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3421 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3481 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3541 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3601 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gcgcttttgg agtacgtcgt
     3661 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     3721 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     3781 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     3841 aggtgtcgtg agctagccca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     3901 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     3961 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4021 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4081 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4141 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4201 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     4261 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     4321 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     4381 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     4441 gacccgcaag cccggtgccg gatcgggaga gggcagagga agtctgctaa catgcggtga
     4501 cgtcgaggag aatcctggcc caccggtcgc caccatggtg agcaagggcg aggagctgtt
     4561 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4621 cgtgtctggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4681 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4741 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4801 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4861 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4921 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4981 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg
     5041 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     5101 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     5161 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     5221 gatcactctc ggcatggacg agctgtacaa gggtggaagt ggtatggcga agaagacgta
     5281 cgacctgctt ttcaagctgc tcctgatcgg ggattccgga gtggggaaga cctgcgtcct
     5341 ttttcgtttt tcggatgatg ccttcaatac tacctttatt tccaccatag gaatagactt
     5401 caagatcaaa acagttgaat tacaaggaaa gaagatcaag ctacagatat gggatacagc
     5461 aggccaggag cgatttcaca ccatcacaac ctcctactac agaggcgcaa tgggtatcat
     5521 gctagtatat gacatcacca atggtaaaag ttttgaaaac atcagcaaat ggcttagaaa
     5581 catagatgag catgccaatg aagatgtgga aagaatgtta ctaggaaaca agtgtgatat
     5641 ggacgacaaa agagttgtac ctaaaggaaa aggagaacag attgcaaggg agcatggtat
     5701 taggtttttt gagactagtg caaaagcaaa tataaacatc gaaaaggcgt tcctcacgtt
     5761 agctgaagat atccttcgaa agacccctgt aaaagagccc aacagtgaaa atgtagatat
     5821 cagcagtgga ggaggcgtga caggctggaa gagcaaatgc tgctgagaat tcgtcgaggg
     5881 acctaataac ttcgtatagc atacattata cgaagttata catgtttaag ggttccggtt
     5941 ccactaggta caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     6001 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6061 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6121 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6181 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6241 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6301 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6361 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     6421 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6481 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6541 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac
     6601 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     6661 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     6721 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6781 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     6841 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     6901 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     6961 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7021 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     7081 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7141 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7201 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7261 aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7321 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7381 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7441 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7501 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7561 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7621 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7681 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7741 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7801 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7861 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7921 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7981 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8041 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8101 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8161 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8221 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8281 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8341 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8401 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8461 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8521 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8581 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8641 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8701 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8761 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8821 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8881 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8941 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9001 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9061 catttccccg aaaagtgcca cctgac
//
