LOCUS Exported 5459 bp ds-DNA circular SYN 16-JUN-2017 DEFINITION GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element. ACCESSION . VERSION . KEYWORDS pAAV-mDlx-GFP-Fishell-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5459) AUTHORS Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G, Fishell G TITLE A viral strategy for targeting and manipulating interneurons across vertebrate species. JOURNAL Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430. PUBMED 27798629 REFERENCE 2 (bases 1 to 5459) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jun 16, 2017 from SnapGene Console 4.0.0a8 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7633 /dnas_title="pAAV-mDlx-PSD95.FingR-CamPARI 2.0-W391-V398-CCR5TC- 2A-Cerulean-Fishell1" /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 12..141 /label=AAV2 ITR primer_bind complement(23..34) /label=L4440 /note="L4440 vector, forward primer" primer_bind 84..95 /label=L4440 /note="L4440 vector, forward primer" intron 860..992 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 975..994 /label=pMT2-F /note="Synthetic intron, forward primer" misc_feature 3979..4567 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4032..4052) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4450..4461) /dnas_title="Factor Xa recognition and cleavage site" /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 4736..4817 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4773..4792) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4827..4846 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" repeat_region 4896..5036 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 4896..5025 /label=AAV2 ITR primer_bind complement(4942..4953) /label=L4440 /note="L4440 vector, forward primer" primer_bind 5003..5014 /label=L4440 /note="L4440 vector, forward primer" rep_origin 5111..5566 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5198..5217) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5408..5429 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5583..5602) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5702..5724 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5762..5780) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5848..5952 /dnas_title="bla" /gene="bla" /label=AmpR promoter CDS 5953..6813 /dnas_title="bla" /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" primer_bind complement(6171..6190) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6984..7572 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7473..7492 /label=pBR322ori-F /note="pBR322 origin, forward primer" PCR_primer complement(678..699) /dnas_title="CTTCCTACTGTGAAACTTTGGG" /label=mDlx enhancer rev primer /primer="CTTCCTACTGTGAAACTTTGGG" /current=0 PCR_primer 170..191 /dnas_title="TATACACTCACAGTGGTTTGGC" /label=mDlx enhancer forw primer /primer="TATACACTCACAGTGGTTTGGC" /current=1 enhancer 170..699 /note="mDlx" misc_feature 1034..3124 /dnas_title="ORF" /vntifkey="21" /label=ORF misc_feature 1034..3124 /dnas_title="PSD95.FingR-CamPARI-CCR5TC" /vntifkey="21" /label=PSD95.FingR-CamPARI-CCR5TC misc_feature 1304..1327 /dnas_title="FLAG" /vntifkey="21" /label=FLAG misc_feature 1349..2567 /dnas_title="CamPARI" /vntifkey="21" /label=CamPARI misc_feature 1796..1840 /dnas_title="mEos Cterm" /vntifkey="21" /label=mEos Cterm misc_feature 1772..1774 /dnas_title="Q142V" /vntifkey="21" /label=Q142V misc_feature 1997..1999 /dnas_title="L217I" /vntifkey="21" /label=L217I misc_feature 1952..1954 /dnas_title="C202T" /vntifkey="21" /label=C202T misc_feature 1940..1942 /dnas_title="F198Y" /vntifkey="21" /label=F198Y intron 2048..2065 /dnas_title="Intron_1" /vntifkey="15" /label=Intron_1 CDS 2066..2497 /dnas_title="CDS_4" /vntifkey="4" /label=CDS_4 misc_feature 2252..2260 /dnas_title="Misc_Feature_12" /vntifkey="21" /label=Misc_Feature_12 misc_feature 2498..2503 /dnas_title="Misc_Feature_14" /vntifkey="21" /label=Misc_Feature_14 misc_feature 2519..2521 /dnas_title="W391" /vntifkey="21" /label=W391 misc_feature 2381..2383 /dnas_title="N345S" /vntifkey="21" /label=N345S misc_feature 2540..2542 /dnas_title="V398" /vntifkey="21" /label=V398 PCR_primer 2472..2494 /note="CamPARI-2c" misc_feature 2600..2952 /dnas_title="CCR5 Left handed ZF" /vntifkey="21" /label=CCR5 Left handed ZF PCR_primer 2943..2963 /note="FingR-1c" misc_feature 3140..3199 /dnas_title="2A" /vntifkey="21" /label=2A PCR_primer complement(3323..3344) /note="Cerulean-CFP-1nc" /note="" misc_feature 3215..3934 /dnas_title="Cerulean CFP" /vntifkey="21" /label=Cerulean CFP PCR_primer 631..652 /note="mDlx-1c" PCR_primer complement(4072..4093) /note="WPRE-2nc" /note="" PCR_primer 847..868 /note="mDlx-2c" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtttaat taagaattct atacactcac 181 agtggtttgg catatatttg gtgaaatttt ttaaggaaaa attagtgttg gtttcgatat 241 atggtagctt tttctctaac ataatttgaa taattcagca aagccctact accagctgta 301 cttctgcagc ctcttccatt ctttccagca ttataatttt ggttaatttt caattttagg 361 tcctacgtct ctgcaatttg tgtatgaata acagaataat ttccctcttt tgtttcgcct 421 ttcctgttcc tgaatctaaa taaagatggc tttttagtat taaaagtgga agaaaattac 481 aggtaattat ctttgacggt aaaaacgctg taatcagcgg gctacatgaa aaattactct 541 aattatggct gcatttaaga gaatggaaaa aaaccttctt gtggataaaa accttaaatt 601 gtccccaatg tctgcttcaa attggatggc actgcagctg gaggctttgt tcagaattga 661 tcctggggag ctacgaaccc aaagtttcac agtaggaagc ttgatatcga attcctgcag 721 cccgggctgg gcataaaagt cagggcagag ccatctattg cttacatttg cttctagcct 781 gcaggtcgag gagcgcagcc ttccagaagc agagcgcggc gccttaagct gcagaagttg 841 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata 901 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct 961 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc 1021 ttaagaaact agcatgctcg aagtcaagga agcatcacca acaagcatcc agatcagctg 1081 ggtgctccac ttgcgccacg ttcgctacta ccgcatcacc tacggtgaaa ctggtggcaa 1141 tagccctgtc caggaattca ccgtgcctgg cagcaagtcc actgctacca tcagcggcct 1201 gaaacctggt gtcgactata ccatcacggt gtacgccgtc acgatcttca gcgcctaccg 1261 ctccgcctgg ccgccgatct ccatcaacta ccgcaccgga accgattaca aggatgacga 1321 cgataagggt agcggctcca gtagatctga ccaactgact gaagagcaga tcgcagaatt 1381 taaagaggct ttctccctat ttgacaagga cggggatggg acaataacaa ccaaggagct 1441 ggggacggtg atgcggtctc tggggcagaa ccccacagaa gcagagctgc aggacatgat 1501 caatgaagta gatgccgacg gtgacggcac aatcgacttc cctgagttcc tgacaatgat 1561 ggcaagaaaa atgaaagaca cagacagtga agaagaaatt agagaagcgt tccgtgtgtt 1621 tgataaggat ggcaatggct acatcagtgc agcagagctt cgccacgtga tgacaaacct 1681 tggagagaag ttaacagatg aagaggttga tgaaatgatc agggaagcag acatcgatgg 1741 ggatggtcag gtaaactacg aagagtttgt agtgatgatg acagcgaagc tcgagtgcga 1801 gaaaatctat gtgcgtgatg gagtgctgac gggtgatatt catatgatct tgttgcttga 1861 aggaaatgcc cattaccgat gtgacttcag aactacttac aaagctaagg agaagggtgt 1921 caagttacca ggcgtgcact atgtggacca caccattgag attttaagcc atgacaaaga 1981 ttacaacaag gttaagatct atgagtatgc tgttgctcat tctggattgc ctgacaatgc 2041 cagacgaggc ggtaccggcg gatccatggt gagtgcgatt aagccagaca tgaagatcaa 2101 actgcgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg 2161 caagccttat gagggaaaac agaccatgga tcttgaagtc aaagagggcg gacctctgcc 2221 ttttgccttt gatatcctga ccactgcatt ccattacggc aacagggtat tcgtgaaata 2281 tccagacaac atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg 2341 aagcatgact ttcgaagacg ggggcatttg ctatgccaga agcgacataa caatggaagg 2401 ggacactttc tataataaag ttcgatttta tggtaccaac tttcccgcca atggtccagt 2461 tatgcagaag aagacgctga aatggatgcc gagctggacg cgttcatcac gtcgtaagtg 2521 gaataagaca ggtcacgcag tcagagctat aggtcggctg gagctcggag ctggcgctgg 2581 agcgggtgca ggggctggct ctagattcca gtgccggatc tgcatgcgga acttcagcga 2641 ccggtccaac ctgagcaggc acatcagaac ccacaccgga gaaaagccct tcgcctgcga 2701 catttgcggc cggaagttcg ccatcagcag caacctgaac agccacacca agatccacac 2761 tggcagccag aaacctttcc agtgcagaat ttgtatgaga aactttagca gaagcgacaa 2821 cctggccaga cacatccgga cacatactgg tgaaaaacct tttgcctgtg atatctgtgg 2881 cagaaagttt gccacctccg gcaatctgac ccggcacaca aagattcacc tgcggggcag 2941 ccagctatcg attgtcgacg ctcctgaaca acgtgaaggt gcttctcaag tttctgtttc 3001 tgttactttt gaagatgttg ctgttctttt tactcgtgat gaatggaaaa aacttgatct 3061 ttctcaacgt tctctttatc gtgaagttat gcttgaaaat tattctaatc ttgcttctat 3121 ggctTAcgta cccgcggccg agggcagagg aagtcttcta acatgcggtg acgtggagga 3181 gaatcccggc cctgcaccgg gatccgatat ccccatggtg agcaagggcg aggagctgtt 3241 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 3301 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 3361 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt 3421 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 3481 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 3541 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 3601 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg ccatcagcga 3661 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg 3721 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 3781 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag 3841 caaagacccc aacgagaagc gcaatcacat ggtcctgctg gagttcgtga ccgccgccgg 3901 gatcactctc ggcatggacg agctgtacaa gtaaaggcgc gccacccctg cagggaattc 3961 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt 4021 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 4081 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 4141 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 4201 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 4261 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 4321 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg 4381 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc 4441 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 4501 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 4561 tccccgcatc gataccgagc gctgctcgag agatctgtga tagcggccat caagctggcc 4621 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 4681 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 4741 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 4801 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcagctta 4861 tcgataccgc atgcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc 4921 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg 4981 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg 5041 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag 5101 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 5161 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 5221 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 5281 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca 5341 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 5401 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct 5461 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 5521 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc 5581 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct 5641 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 5701 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag 5761 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 5821 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 5881 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 5941 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 6001 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 6061 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 6121 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 6181 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 6241 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 6301 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 6361 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 6421 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 6481 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 6541 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 6601 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 6661 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 6721 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 6781 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 6841 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 6901 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 6961 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 7021 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 7081 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 7141 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 7201 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 7261 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 7321 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 7381 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 7441 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 7501 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 7561 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 7621 tttgctcaca tgt //