LOCUS       KT725395               11112 bp    DNA     circular SYN 28-NOV-2015
DEFINITION  Cloning vector pHCT2, complete sequence.
ACCESSION   KT725395
VERSION     KT725395.1  GI:954037492
KEYWORDS    .
SOURCE      Cloning vector pHCT2
  ORGANISM  Cloning vector pHCT2
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Alexander,W.G., Peris,D., Pfannenstiel,B.T., Opulente,D.A.,
            Kuang,M. and Hittinger,C.T.
  TITLE     Efficient engineering of marker-free synthetic allotetraploids of
            Saccharomyces
  JOURNAL   Fungal Genet. Biol. (2015) In press
   PUBMED   26555931
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11112)
  AUTHORS   Alexander,W.G., Peris,D., Pfannenstiel,B., Opulente,D.A., Kuang,M.
            and Hittinger,C.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2015) Laboratory of Genetics, University of
            Wisconsin-Madison, 425-G Henry Mall, 4350 Genetics Biotechnology
            Center, Madison, WI 53706, USA
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(28..1035)
                     /dnas_title="tetracycline-responsive transcription
                     activator"
                     /gene="rtTA"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline-responsive transcription activator"
                     /protein_id="ALP32061.1"
                     /db_xref="GI:954037493"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNY
                     GSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDV
                     AMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYG
                     G"
     regulatory      complement(1140..1343)
                     /regulatory_class="promoter"
     regulatory      complement(1344..1723)
                     /regulatory_class="enhancer"
     regulatory      2044..2491
                     /regulatory_class="promoter"
     CDS             2510..4960
                     /gene="HO"
                     /codon_start=1
                     /transl_table=11
                     /product="homothallic switching endonuclease"
                     /protein_id="ALP32064.1"
                     /db_xref="GI:954037496"
                     /translation="MLSENTTILMANGEIKDIANVTANSYVMCADGSAARVINVTQGY
                     QKIYNIQQKTKHRAFEGEPGRLDPRRRTVYQRLALQCTAGHKLSVRVPTKPLLEKSGR
                     NATKYKVRWRNLQQCQTLDGRIIIIPKNHHKTFPMTVEGEFAAKRFIEEMERSKGEYF
                     NFDIEVRDLDYLDAQLRISSCIRFGPVLAGNGVLSKFLTGRSDLVTPAVKSMAWMLGL
                     WLGDSTTKEPEISVDSLDPKLMESLRENAKIWGLYLTVCDDHVPLRAKHVRLHYGDGP
                     DENRKTRNLRKNNPFWKAVTILKFKRDLDGEKQIPEFMYGEHIEVREAFLAGLIDSDG
                     YVVKKGEGPESYKIAIQTVYSSIMDGIVHISRSLGMSATVTTRSAREEIIEGRKVQCQ
                     FTYDCNVAGGTTSQNVLSYCRSGHKTREVPPIIKREPVYFSFTDDFQGESTVYGLTIE
                     GHKNFLLGNKIEVKSCRGCCVGEQLKISQKKNLKHCVACPRKGIKYFYKDWSGKNRVC
                     ARCYGRYKFSGHHCINCKYVPEAREVKKAKDKGEKLGITPEGLPVKGPECIKCGGILQ
                     FDAVRGPHKSCGNNAGARICRIPGLINGGGGGGGGGEQKLISEEDLNGEQKLISEEDL
                     NGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQ
                     KLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEED
                     LNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGE
                     QKLISEEDLNGEQKLINH"
     misc_feature    4292..4315
                     /note="6x glycine linker"
     misc_feature    4316..4927
                     /note="16x myc epitope tag"
     rep_origin      5676..6276
                     /note="colE1 ori"
     CDS             complement(6437..7297)
                     /gene="ampR"
                     /note="ampicillin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="ALP32062.1"
                     /db_xref="GI:954037494"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      7570..7949
                     /regulatory_class="TEF promoter"
     CDS             7954..8526
                     /gene="natR"
                     /codon_start=1
                     /transl_table=11
                     /product="nourseothricin-resistance"
                     /protein_id="ALP32063.1"
                     /db_xref="GI:954037495"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP"
     regulatory      8527..8793
                     /regulatory_class="TEF terminator"
     rep_origin      8794..11112
                     /note="CEN4/ARS416"
     source          1..11112
                     /dnas_title="pHCT2_ed"
ORIGIN      
        1 gaattcttat tacgatcctc gcgcccccta cccaccgtac tcgtcaattc caagggcatc
       61 ggtaaacatc tgctcaaact cgaagtcggc catatccaga gcgccgtagg gggcggagtc
      121 gtggggggta aatcccggac ccggggaatc cccgtccccc aacatgtcca gatcgaaatc
      181 gtctagcgcg tcggcatgcg ccatcgccac gtcctcgccg tctaagtgga gctcgtcccc
      241 caggctgaca tcggtcgggg gggccgtcga cagtctgcgc gtgtgtcccg cggggagaaa
      301 ggacaggcgc ggagccgcca gccccgcctc ttcgggggcg tcgtcgtccg ggagatcgag
      361 caggccctcg atggtagacc cgtaattgtt tttcgtacgc gcgcggctgt acgcggaccc
      421 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
      481 ggctggctct gcaccttggc gatcaaataa ttcgatagct tgtcgtaata atggcggcat
      541 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gttcttccaa
      601 tacgcaacct aaagtaaagt gccccacagc gctgagtgca tataatgcat tctctagtga
      661 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
      721 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
      781 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      841 agtatggtgc ctatctaaca tctcaatggg taaggcgtcg agcaaagccc gcttattttt
      901 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      961 taaaccttcg attccgaccc cattaagcag ctctaatgcg ccgttaatca ctttactttt
     1021 atctaatcta gacatatgaa ttaattcggg ccgcggaggc tggatcggtc ccggtgtctt
     1081 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
     1141 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     1201 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     1261 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     1321 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     1381 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     1441 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     1501 gcagtttagc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     1561 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     1621 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     1681 cccgtaattg attactatta ataactagtc aataatcaat gtcaacatgg cggtaatgtt
     1741 ggacatgagc caatataaat gtacatatta tgatatggat acaacgtatg caatgggcca
     1801 agctcctcga gtaattcgcg ccacttctaa ataagcgaat ttcttatgat ttatgatttt
     1861 tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt
     1921 tttaaaacga aaattcttgt tcttgagtaa ctctttcctg taggtcaggt tgctttctca
     1981 ggtatagcat gaggtcgctc ttattgacca cacctctacc ggcagatcaa ttcctcgatc
     2041 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2101 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2161 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2221 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2281 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     2341 ggtaccctat ggcatgcatg tgctctgtat gtatataaaa ctcttgtttt cttcttttct
     2401 ctaaatattc tttccttata cattaggtcc tttgtagcat aaattactat acttctatag
     2461 acacgcaaac acaaatacac acactaaatt accggatcaa ttcgggggaa tgctttctga
     2521 aaacacgact attctgatgg ctaacggtga aattaaagac atcgcaaacg tcacggctaa
     2581 ctcttacgtt atgtgcgcag atggctccgc tgcccgcgtc ataaatgtca cacagggcta
     2641 tcagaaaatc tataatatac agcaaaaaac caaacacaga gcttttgaag gtgaacctgg
     2701 taggttagat cccaggcgta gaacagttta tcagcgtctt gcattacaat gtactgcagg
     2761 tcataaattg tcagtcaggg tccctaccaa accactgttg gaaaaaagtg gtagaaatgc
     2821 caccaaatat aaagtgagat ggagaaatct gcagcaatgt cagacgcttg atggtaggat
     2881 aataataatt ccaaaaaacc atcataagac attcccaatg acagttgaag gtgagtttgc
     2941 cgcaaaacgc ttcatagaag aaatggagcg ctctaaagga gaatatttca actttgacat
     3001 tgaagttaga gatttggatt atcttgatgc tcaattgaga atttctagct gcataagatt
     3061 tggtccagta ctcgcaggaa atggtgtttt atctaaattt ctcactggac gtagtgacct
     3121 tgtaactcct gctgtaaaaa gtatggcttg gatgcttggt ctgtggttag gtgacagtac
     3181 aacaaaagag ccagaaatct cagtagatag cttggatcct aagctaatgg agagtttaag
     3241 agaaaatgcg aaaatctggg gtctctacct tacggtttgt gacgatcacg ttccgctacg
     3301 tgccaaacat gtaaggcttc attatggaga tggtccagat gaaaacagga agacaaggaa
     3361 tttgaggaaa aataatccat tctggaaagc tgtcacaatt ttaaagttta aaagggatct
     3421 tgatggagag aagcaaatcc ctgaatttat gtacggcgag catatagaag ttcgtgaagc
     3481 attcttagcc ggcttgatcg actcagatgg gtacgttgtg aaaaagggcg aaggccctga
     3541 atcttataaa atagcaattc aaactgttta ttcatccatt atggacggaa ttgtccatat
     3601 ttcaagatct cttggtatgt cagctactgt gacgaccagg tcagctaggg aggaaatcat
     3661 tgaaggaaga aaagtccaat gtcaatttac atacgactgt aatgttgctg ggggaacaac
     3721 ttcacagaat gttttgtcat attgtcgaag tggtcacaaa acaagagaag ttccgccaat
     3781 tataaaaagg gaacccgtat atttcagctt cacggatgat ttccagggtg agagtactgt
     3841 atatgggctt acgatagaag gccataaaaa tttcttgctt ggcaacaaaa tagaagtgaa
     3901 atcatgtcga ggctgctgtg tgggagaaca gcttaaaata tcacaaaaaa agaatctaaa
     3961 acactgtgtt gcttgtccca gaaagggaat caagtatttt tataaagatt ggagtggtaa
     4021 aaatcgagta tgtgctagat gctatggaag atacaaattc agcggtcatc actgtataaa
     4081 ttgcaagtat gtaccagaag cacgtgaagt gaaaaaggca aaagacaaag gcgaaaaatt
     4141 gggcattacg cccgaaggtt tgccagttaa aggaccagag tgtataaaat gtggcggaat
     4201 cttacagttt gatgctgtcc gcgggcctca taagagttgt ggtaacaacg caggtgcgcg
     4261 catctgccgg atccccgggt taattaacgg tggaggtgga ggtggaggtg gaggtgaaca
     4321 aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt
     4381 gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa
     4441 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
     4501 cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct
     4561 aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg
     4621 actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat
     4681 ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact tgaacggact
     4741 cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc
     4801 cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa gaagacttga acggactcga
     4861 cggtgaacaa aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga
     4921 ggaagacttg aacggtgaac aaaaattaat caatcactag tgaattcgcg ccacttctaa
     4981 ataagcgaat ttcttgaggg ccgcatcatg taattagtta tgtcacgctt acattcacgc
     5041 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     5101 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     5161 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
     5221 aaggttttgg gacgctcgaa ggctttaatt tgcggccaag cttggcgtaa tcatggtcat
     5281 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5341 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5401 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5461 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6721 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7021 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7441 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     7501 aagaattcag ctgaagcttc gtacgctgca ggtcgacgga tccccgggtt aattaaggcg
     7561 cgccagatct gtttagcttg ccttgtcccc gccgggtcac ccggccagcg acatggaggc
     7621 ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg
     7681 cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg
     7741 ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc
     7801 tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg
     7861 ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata
     7921 cagttctcac atcacatccg aacataaaca accatgggta ccactcttga cgacacggct
     7981 taccggtacc gcaccagtgt cccgggggac gccgaggcca tcgaggcact ggatgggtcc
     8041 ttcaccaccg acaccgtctt ccgcgtcacc gccaccgggg acggcttcac cctgcgggag
     8101 gtgccggtgg acccgcccct gaccaaggtg ttccccgacg acgaatcgga cgacgaatcg
     8161 gacgacgggg aggacggcga cccggactcc cggacgttcg tcgcgtacgg ggacgacggc
     8221 gacctggcgg gcttcgtggt cgtctcgtac tccggctgga accgccggct gaccgtcgag
     8281 gacatcgagg tcgccccgga gcaccggggg cacggggtcg ggcgcgcgtt gatggggctc
     8341 gcgacggagt tcgcccgcga gcggggcgcc gggcacctct ggctggaggt caccaacgtc
     8401 aacgcaccgg cgatccacgc gtaccggcgg atggggttca ccctctgcgg cctggacacc
     8461 gccctgtacg acggcaccgc ctcggacggc gagcaggcgc tctacatgag catgccctgc
     8521 ccctaatcag tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt
     8581 ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc
     8641 gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca
     8701 atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag
     8761 tgtcgaaaac gagctcgaat tcatcgatga tatcagatcc actagtggcc tatgcaattc
     8821 tcatgtttga cagcttatca tcgatcgtcc aactgcatgg agatgagtcg tggcaagaat
     8881 accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa gactgcaaca
     8941 tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt gattcagaag
     9001 caggtgggac aggtgaactt ttggattgga actcgatttc tgactgggtt ggaaggcaag
     9061 agagccccga gagcttacat tttatgttag ctggtggact gacgccagaa aatgttggtg
     9121 atgcgcttag attaaatggc gttattggtg ttgatgtaag cggaggtgtg gagacaaatg
     9181 gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta
     9241 ctgagtagta tttatttaag tattgtttgt gcacttgcct gcaagccttt tgaaaagcaa
     9301 gcataaaaga tctaaacata aaatctgtaa aataacaaga tgtaaagata atgctaaatc
     9361 atttggcttt ttgattgatt gtacaggaaa atatacatcg cagggggttg acttttacca
     9421 tttcaccgca atggaatcaa acttgttgaa gagaatgttc acaggcgcat acgctacaat
     9481 gacccgattc ttgctagcct tttctcggtc ttgcaaacaa ccgccggcag cttagtatat
     9541 aaatacacat gtacatacct ctctccgtat cctcgtaatc attttcttgt atttatcgtc
     9601 ttttcgctgt aaaaacttta tcacacttat ctcaaataca cttattaacc gcttttacta
     9661 ttatcttcta cgctgacagt aatatcaaac agtgacacat attaaacaca gtggtttctt
     9721 tgcataaaca ccatcagcct caagtcgtca agtaaagatt tcgtgttcat gcagatagat
     9781 aacaatctat atgttgataa ttagcgttgc ctcatcaatg cgagatccgt ttaaccggac
     9841 cctagtgcac ttaccccacg ttcggtccac tgtgtgccga acatgctcct tcactatttt
     9901 aacatgtgga attatcgatg ccggtgttat tcttgctatg ataggccatc gaatgttaaa
     9961 gggaagattt attgttttgc cctttaaaga cgaatttgga ccgtgaatgt ttaaataatt
    10021 ctcatgtttg acagcttatc atcgaactct aagaggtgat acttatttac tgtaaaactg
    10081 tgacgataaa accggaagga agaataagaa aactcgaact gatctataat gcctattttc
    10141 tgtaaagagt ttaagctatg aaagcctcgg cattttggcc gctcctaggt agtgcttttt
    10201 ttccaaggac aaaacagttt ctttttcttg agcaggtttt atgtttcggt aatcataaac
    10261 aataaataaa ttatttcatt tatgtttaaa aataaaaaat aaaaaagtat tttaaatttt
    10321 taaaaaagtt gattataagc atgtgacctt ttgcaagcaa ttaaattttg caatttgtga
    10381 ttttaggcaa aagttacaat ttctggctcg tgtaatatat gtatgctaaa gtgaactttt
    10441 acaaagtcga tatggactta gtcaaaagaa attttcttaa aaatatatag cactagccaa
    10501 tttagcactt ctttatgaga tatattatag actttattaa gccagatttg tgtattatat
    10561 gtatttaccc ggcgaatcat ggacatacat tctgaaatag gtaatattct ctatggtgag
    10621 acagcataga taacctagga tacaagttaa aagctagtac tgttttgcag taattttttt
    10681 cttttttata agaatgttac cacctaaata agttataaag tcaatagtta agtttgatat
    10741 ttgattgtaa aataccgtaa tatatttgca tgatcaaaag gctcaatgtt gactagccag
    10801 catgtcaacc actatattga tcaccgatat atggacttcc acaccaacta gtaatatgac
    10861 aataaattca agatattctt catgagaatg gcccagcgat atatgcggtg tgaaataccg
    10921 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
    10981 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    11041 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    11101 acgacggcca gt
//

