LOCUS       KT781077               11568 bp    DNA     circular SYN 19-NOV-2015
DEFINITION  Yeast integrative vector pHMK34, complete sequence.
ACCESSION   KT781077
VERSION     KT781077.1  GI:941350498
KEYWORDS    .
SOURCE      Yeast integrative vector pHMK34
  ORGANISM  Yeast integrative vector pHMK34
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Alexander,W.G., Peris,D., Pfannenstiel,B.T., Opulente,D.A.,
            Kuang,M. and Hittinger,C.T.
  TITLE     Efficient engineering of marker-free synthetic allotetraploids of
            Saccharomyces
  JOURNAL   Fungal Genet. Biol. (2015) In press
   PUBMED   26555931
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11568)
  AUTHORS   Alexander,W.G., Peris,D., Pfannenstiel,B., Opulente,D.A., Kuang,M.
            and Hittinger,C.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2015) Laboratory of Genetics, University of
            Wisconsin-Madison, 425-G Henry Mall, 4350 Genetics Biotechnology
            Center, Madison, WI 53706, USA
FEATURES             Location/Qualifiers
     CDS             complement(28..1035)
                     /dnas_title="tetracycline-responsive transcriptional
                     activator"
                     /gene="rtTA"
                     /note="tetracycline-responsive transcriptional activator"
                     /codon_start=1
                     /transl_table=11
                     /product="rtTA"
                     /protein_id="ALK44378.1"
                     /db_xref="GI:941350499"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNY
                     GSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDV
                     AMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYG
                     G"
     regulatory      complement(1140..1343)
                     /regulatory_class="promoter"
     regulatory      complement(1334..1723)
                     /regulatory_class="enhancer"
     rep_origin      5552..6152
                     /note="colE1 ori"
     CDS             complement(6313..7173)
                     /gene="ampR"
                     /note="beta-lactamase"
                     /codon_start=1
                     /transl_table=11
                     /product="ampR"
                     /protein_id="ALK44380.1"
                     /db_xref="GI:941350501"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      7446..7825
                     /regulatory_class="TEF promoter"
     CDS             7830..8858
                     /dnas_title="hygromycin B phosphotransferase"
                     /gene="hph"
                     /note="hygromycin B phosphotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="hph"
                     /protein_id="ALK44381.1"
                     /db_xref="GI:941350502"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGG
                     RGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQ
                     DLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHV
                     YHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDW
                     SEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLY
                     QSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRP
                     STRPRAKE"
     regulatory      8859..9125
                     /regulatory_class="TEF terminator"
     rep_origin      9126..11444
                     /note="CEN/ARS"
     CDS             2510..4960
                     /gene="HO"
                     /codon_start=1
                     /transl_table=11
                     /product="homothallic switching endonuclease"
                     /protein_id="ALP32064.1"
                     /db_xref="GI:954037496"
                     /translation="MLSENTTILMANGEIKDIANVTANSYVMCADGSAARVINVTQGY
                     QKIYNIQQKTKHRAFEGEPGRLDPRRRTVYQRLALQCTAGHKLSVRVPTKPLLEKSGR
                     NATKYKVRWRNLQQCQTLDGRIIIIPKNHHKTFPMTVEGEFAAKRFIEEMERSKGEYF
                     NFDIEVRDLDYLDAQLRISSCIRFGPVLAGNGVLSKFLTGRSDLVTPAVKSMAWMLGL
                     WLGDSTTKEPEISVDSLDPKLMESLRENAKIWGLYLTVCDDHVPLRAKHVRLHYGDGP
                     DENRKTRNLRKNNPFWKAVTILKFKRDLDGEKQIPEFMYGEHIEVREAFLAGLIDSDG
                     YVVKKGEGPESYKIAIQTVYSSIMDGIVHISRSLGMSATVTTRSAREEIIEGRKVQCQ
                     FTYDCNVAGGTTSQNVLSYCRSGHKTREVPPIIKREPVYFSFTDDFQGESTVYGLTIE
                     GHKNFLLGNKIEVKSCRGCCVGEQLKISQKKNLKHCVACPRKGIKYFYKDWSGKNRVC
                     ARCYGRYKFSGHHCINCKYVPEAREVKKAKDKGEKLGITPEGLPVKGPECIKCGGILQ
                     FDAVRGPHKSCGNNAGARICRIPGLINGGGGGGGGGEQKLISEEDLNGEQKLISEEDL
                     NGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQ
                     KLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEED
                     LNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGE
                     QKLISEEDLNGEQKLINH"
     misc_feature    4292..4315
                     /note="6x glycine linker"
     misc_feature    4316..4927
                     /note="16x myc epitope tag"
     regulatory      2044..2491
                     /regulatory_class="promoter"
     source          1..11444
                     /dnas_title="pHMK34_ed"
ORIGIN      
        1 gaattcttat tacgatcctc gcgcccccta cccaccgtac tcgtcaattc caagggcatc
       61 ggtaaacatc tgctcaaact cgaagtcggc catatccaga gcgccgtagg gggcggagtc
      121 gtggggggta aatcccggac ccggggaatc cccgtccccc aacatgtcca gatcgaaatc
      181 gtctagcgcg tcggcatgcg ccatcgccac gtcctcgccg tctaagtgga gctcgtcccc
      241 caggctgaca tcggtcgggg gggccgtcga cagtctgcgc gtgtgtcccg cggggagaaa
      301 ggacaggcgc ggagccgcca gccccgcctc ttcgggggcg tcgtcgtccg ggagatcgag
      361 caggccctcg atggtagacc cgtaattgtt tttcgtacgc gcgcggctgt acgcggaccc
      421 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
      481 ggctggctct gcaccttggc gatcaaataa ttcgatagct tgtcgtaata atggcggcat
      541 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gttcttccaa
      601 tacgcaacct aaagtaaagt gccccacagc gctgagtgca tataatgcat tctctagtga
      661 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
      721 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
      781 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      841 agtatggtgc ctatctaaca tctcaatggg taaggcgtcg agcaaagccc gcttattttt
      901 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      961 taaaccttcg attccgaccc cattaagcag ctctaatgcg ccgttaatca ctttactttt
     1021 atctaatcta gacatatgaa ttaattcggg ccgcggaggc tggatcggtc ccggtgtctt
     1081 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
     1141 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     1201 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     1261 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     1321 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     1381 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     1441 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     1501 gcagtttagc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     1561 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     1621 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     1681 cccgtaattg attactatta ataactagtc aataatcaat gtcaacatgg cggtaatgtt
     1741 ggacatgagc caatataaat gtacatatta tgatatggat acaacgtatg caatgggcca
     1801 agctcctcga gtaattcgcg ccacttctaa ataagcgaat ttcttatgat ttatgatttt
     1861 tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt
     1921 tttaaaacga aaattcttgt tcttgagtaa ctctttcctg taggtcaggt tgctttctca
     1981 ggtatagcat gaggtcgctc ttattgacca cacctctacc ggcagatcaa ttcctcgatc
     2041 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2101 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2161 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2221 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2281 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     2341 ggtaccctat ggcatgcatg tgctctgtat gtatataaaa ctcttgtttt cttcttttct
     2401 ctaaatattc tttccttata cattaggtcc tttgtagcat aaattactat acttctatag
     2461 acacgcaaac acaaatacac acactaaatt accggatcaa ttcgggggaa tgctttctga
     2521 aaacacgact attctgatgg ctaacggtga aattaaagac atcgcaaacg tcacggctaa
     2581 ctcttacgtt atgtgcgcag atggctccgc tgcccgcgtc ataaatgtca cacagggcta
     2641 tcagaaaatc tataatatac agcaaaaaac caaacacaga gcttttgaag gtgaacctgg
     2701 taggttagat cccaggcgta gaacagttta tcagcgtctt gcattacaat gtactgcagg
     2761 tcataaattg tcagtcaggg tccctaccaa accactgttg gaaaaaagtg gtagaaatgc
     2821 caccaaatat aaagtgagat ggagaaatct gcagcaatgt cagacgcttg atggtaggat
     2881 aataataatt ccaaaaaacc atcataagac attcccaatg acagttgaag gtgagtttgc
     2941 cgcaaaacgc ttcatagaag aaatggagcg ctctaaagga gaatatttca actttgacat
     3001 tgaagttaga gatttggatt atcttgatgc tcaattgaga atttctagct gcataagatt
     3061 tggtccagta ctcgcaggaa atggtgtttt atctaaattt ctcactggac gtagtgacct
     3121 tgtaactcct gctgtaaaaa gtatggcttg gatgcttggt ctgtggttag gtgacagtac
     3181 aacaaaagag ccagaaatct cagtagatag cttggatcct aagctaatgg agagtttaag
     3241 agaaaatgcg aaaatctggg gtctctacct tacggtttgt gacgatcacg ttccgctacg
     3301 tgccaaacat gtaaggcttc attatggaga tggtccagat gaaaacagga agacaaggaa
     3361 tttgaggaaa aataatccat tctggaaagc tgtcacaatt ttaaagttta aaagggatct
     3421 tgatggagag aagcaaatcc ctgaatttat gtacggcgag catatagaag ttcgtgaagc
     3481 attcttagcc ggcttgatcg actcagatgg gtacgttgtg aaaaagggcg aaggccctga
     3541 atcttataaa atagcaattc aaactgttta ttcatccatt atggacggaa ttgtccatat
     3601 ttcaagatct cttggtatgt cagctactgt gacgaccagg tcagctaggg aggaaatcat
     3661 tgaaggaaga aaagtccaat gtcaatttac atacgactgt aatgttgctg ggggaacaac
     3721 ttcacagaat gttttgtcat attgtcgaag tggtcacaaa acaagagaag ttccgccaat
     3781 tataaaaagg gaacccgtat atttcagctt cacggatgat ttccagggtg agagtactgt
     3841 atatgggctt acgatagaag gccataaaaa tttcttgctt ggcaacaaaa tagaagtgaa
     3901 atcatgtcga ggctgctgtg tgggagaaca gcttaaaata tcacaaaaaa agaatctaaa
     3961 acactgtgtt gcttgtccca gaaagggaat caagtatttt tataaagatt ggagtggtaa
     4021 aaatcgagta tgtgctagat gctatggaag atacaaattc agcggtcatc actgtataaa
     4081 ttgcaagtat gtaccagaag cacgtgaagt gaaaaaggca aaagacaaag gcgaaaaatt
     4141 gggcattacg cccgaaggtt tgccagttaa aggaccagag tgtataaaat gtggcggaat
     4201 cttacagttt gatgctgtcc gcgggcctca taagagttgt ggtaacaacg caggtgcgcg
     4261 catctgccgg atccccgggt taattaacgg tggaggtgga ggtggaggtg gaggtgaaca
     4321 aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt
     4381 gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa
     4441 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
     4501 cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct
     4561 aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg
     4621 actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat
     4681 ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact tgaacggact
     4741 cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc
     4801 cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa gaagacttga acggactcga
     4861 cggtgaacaa aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga
     4921 ggaagacttg aacggtgaac aaaaattaat caatcactag acctgaagtc taggtcccta
     4981 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
     5041 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg
     5101 ttttgggacg ctcgaaggct ttaatttgcg gccaagcttg gcgtaatcat ggtcatagct
     5161 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5221 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5281 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5341 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5401 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5461 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5521 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5581 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5641 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5701 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5761 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5821 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5881 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5941 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     6001 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6061 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6121 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6181 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6241 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6301 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6361 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6421 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6481 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6541 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6601 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6661 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6721 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6781 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6841 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6901 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6961 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7021 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7081 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7141 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7201 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7261 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     7321 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     7381 attcagctga agcttcgtac gctgcaggtc gacggatccc cgggttaatt aaggcgcgcc
     7441 agatctgttt agcttgcctt gtccccgccg ggtcacccgg ccagcgacat ggaggcccag
     7501 aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg
     7561 tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc
     7621 acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc
     7681 ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag
     7741 gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt
     7801 tctcacatca catccgaaca taaacaacca tgggtaaaaa gcctgaactc accgcgacgt
     7861 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     7921 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     7981 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     8041 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     8101 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     8161 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     8221 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     8281 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     8341 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     8401 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     8461 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     8521 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     8581 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     8641 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     8701 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     8761 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     8821 accgacgccc cagcactcgt ccgagggcaa aggaataatc agtactgaca ataaaaagat
     8881 tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta
     8941 atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa
     9001 gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact
     9061 gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa acgagctcga attcatcgat
     9121 gatatcagat ccactagtgg cctatgcaat tctcatgttt gacagcttat catcgatcgt
     9181 ccaactgcat ggagatgagt cgtggcaaga ataccaagag ttcctcggtt tgccagttat
     9241 taaaagactc gtatttccaa aagactgcaa catactactc agtgcagctt cacagaaacc
     9301 tcattcgttt attcccttgt ttgattcaga agcaggtggg acaggtgaac ttttggattg
     9361 gaactcgatt tctgactggg ttggaaggca agagagcccc gagagcttac attttatgtt
     9421 agctggtgga ctgacgccag aaaatgttgg tgatgcgctt agattaaatg gcgttattgg
     9481 tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa
     9541 tttcgtcaaa aatgctaaga aataggttat tactgagtag tatttattta agtattgttt
     9601 gtgcacttgc ctgcaagcct tttgaaaagc aagcataaaa gatctaaaca taaaatctgt
     9661 aaaataacaa gatgtaaaga taatgctaaa tcatttggct ttttgattga ttgtacagga
     9721 aaatatacat cgcagggggt tgacttttac catttcaccg caatggaatc aaacttgttg
     9781 aagagaatgt tcacaggcgc atacgctaca atgacccgat tcttgctagc cttttctcgg
     9841 tcttgcaaac aaccgccggc agcttagtat ataaatacac atgtacatac ctctctccgt
     9901 atcctcgtaa tcattttctt gtatttatcg tcttttcgct gtaaaaactt tatcacactt
     9961 atctcaaata cacttattaa ccgcttttac tattatcttc tacgctgaca gtaatatcaa
    10021 acagtgacac atattaaaca cagtggtttc tttgcataaa caccatcagc ctcaagtcgt
    10081 caagtaaaga tttcgtgttc atgcagatag ataacaatct atatgttgat aattagcgtt
    10141 gcctcatcaa tgcgagatcc gtttaaccgg accctagtgc acttacccca cgttcggtcc
    10201 actgtgtgcc gaacatgctc cttcactatt ttaacatgtg gaattatcga tgccggtgtt
    10261 attcttgcta tgataggcca tcgaatgtta aagggaagat ttattgtttt gccctttaaa
    10321 gacgaatttg gaccgtgaat gtttaaataa ttctcatgtt tgacagctta tcatcgaact
    10381 ctaagaggtg atacttattt actgtaaaac tgtgacgata aaaccggaag gaagaataag
    10441 aaaactcgaa ctgatctata atgcctattt tctgtaaaga gtttaagcta tgaaagcctc
    10501 ggcattttgg ccgctcctag gtagtgcttt ttttccaagg acaaaacagt ttctttttct
    10561 tgagcaggtt ttatgtttcg gtaatcataa acaataaata aattatttca tttatgttta
    10621 aaaataaaaa ataaaaaagt attttaaatt tttaaaaaag ttgattataa gcatgtgacc
    10681 ttttgcaagc aattaaattt tgcaatttgt gattttaggc aaaagttaca atttctggct
    10741 cgtgtaatat atgtatgcta aagtgaactt ttacaaagtc gatatggact tagtcaaaag
    10801 aaattttctt aaaaatatat agcactagcc aatttagcac ttctttatga gatatattat
    10861 agactttatt aagccagatt tgtgtattat atgtatttac ccggcgaatc atggacatac
    10921 attctgaaat aggtaatatt ctctatggtg agacagcata gataacctag gatacaagtt
    10981 aaaagctagt actgttttgc agtaattttt ttctttttta taagaatgtt accacctaaa
    11041 taagttataa agtcaatagt taagtttgat atttgattgt aaaataccgt aatatatttg
    11101 catgatcaaa aggctcaatg ttgactagcc agcatgtcaa ccactatatt gatcaccgat
    11161 atatggactt ccacaccaac tagtaatatg acaataaatt caagatattc ttcatgagaa
    11221 tggcccagcg atatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    11281 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    11341 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    11401 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagt
//

