LOCUS       Exported                8819 bp ds-DNA     circular SYN 23-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 23, 2019 from SnapGene Viewer 4.3.2
            https://www.snapgene.com
COMMENT     [DEFINITION]: . [LOCUS]: pFastM13_cterm_K [ACCESSION]:
FEATURES             Location/Qualifiers
     source          1..8819
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             589..1449
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3524..3552)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4062..4079
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4101..4121
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4143..7448
                     /codon_start=1
                     /label=hsKIF1C
                     /translation="AGASVKVAVRVRPFNARETSQDAKCVVSMQGNTTSIINPKQSKDA
                     PKSFTFDYSYWSHTSTEDPQFASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSY
                     TMMGRQEPGQQGIVPQLCEDLFSRVSENQSAQLSYSVEVSYMEIYCERVRDLLNPKSRG
                     SLRVREHPILGPYVQDLSKLAVTSYADIADLMDCGNKARTVAATNMNETSSRSHAVFTI
                     VFTQRCHDQLTGLDSEKVSKISLVDLAGSERADSSGARGMRLKEGANINKSLTTLGKVI
                     SALADMQSKKRKSDFIPYRDSVLTWLLKENLGGNSRTAMIAALSPADINYEETLSTLRY
                     ADRTKQIRCNAIINEDPNARLIRELQEEVARLRELLMAQGLSASALEGLKTEEGSVRGA
                     LPAVSSPPAPVSPSSPTTHNGELEPSFSPNTESQIGPEEAMERLQETEKIIAELNETWE
                     EKLRKTEALRMEREALLAEMGVAVREDGGTVGVFSPKKTPHLVNLNEDPLMSECLLYHI
                     KDGVTRVGQVDMDIKLTGQFIREQHCLFRSIPQPDGEVVVTLEPCEGAETYVNGKLVTE
                     PLVLKSGNRIVMGKNHVFRFNHPEQARLERERGVPPPPGPPSEPVDWNFAQKELLEQQG
                     IDIKLEMEKRLQDLENQYRKEKEEADLLLEQQRLYADSDSGDDSDKRSCEESWRLISSL
                     REQLPPTTVQTIVKRCGLPSSGKRRAPRRVYQIPQRRRLQGKDPRWATMADLKMQAVKE
                     ICYEVALADFRHGRAEIEALAALKMRELCRTYGKPDGPGDAWRAVARDVWDTVGEEEGG
                     GAGSGGGSEEGARGAEVEDLRAHIDKLTGILQEVKLQNSSKDRELQALRDRMLRMERVI
                     PLAQDHEDENEEGGEVPWAPPEGSEAAEEAAPSDRMPSARPPSPPLSSWERVSRLMEED
                     PAFRRGRLRWLKQEQLRLQGLQGSGGRGGGLRRPPARFVPPHDCKLRFPFKSNPQHRES
                     WPGMGSGEAPTPLQPPEEVTPHPATPARRPPSPRRSHHPRRNSLDGGGRSRGAGSAQPE
                     PQHFQPKKHNSYPQPPQPYPAQRPPGPRYPPYTTPPRMRRQRSAPDLKESGAAV"
     regulatory      6363..6372
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      7461..7470
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7467..8186
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    8309..8443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  8472..8637
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
     4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggcg cgcccggatc
     4141 tggctggtgc ctcggtgaaa gtggcagtga gggttcggcc ctttaacgcc cgtgagacca
     4201 gccaggatgc caagtgtgtg gtcagcatgc agggcaacac cacctccatc atcaatccta
     4261 aacagagcaa ggatgccccc aaaagcttca cctttgacta ctcctactgg tcacacactt
     4321 cgacggagga cccccagttt gcatctcagc agcaagtgta tcgggacatc ggagaagaga
     4381 tgctgctcca cgcctttgaa ggctacaacg tgtgcatctt tgcctatggg cagaccgggg
     4441 ctgggaaatc ctataccatg atggggcgac aggagccagg gcagcagggc atcgtgcccc
     4501 agctctgtga ggacctcttc tctcgcgtta gtgagaacca gagtgctcag ctatcctact
     4561 ctgtggaggt gagctatatg gagatctact gtgagcgggt acgagacctc ttgaacccca
     4621 agagtcgggg ttctctgcgg gtccgggagc accccatcct gggcccgtac gtgcaggacc
     4681 tgtccaaatt ggctgtgacc tcctacgcag acattgctga cctcatggac tgtggaaata
     4741 aagcacggac tgtggctgcc accaacatga atgagaccag cagccgttcc catgccgtct
     4801 ttaccatcgt cttcacacag cgctgccatg accagctcac ggggctggac tcggagaagg
     4861 tcagtaagat cagtttggtg gaccttgctg ggagtgagcg agccgactcc tcaggggccc
     4921 ggggcatgcg cctgaaggaa ggagccaaca tcaataagtc cctgactaca ctagggaaag
     4981 tgatctcggc ccttgcagat atgcaatcaa agaagcgaaa gtcggatttt atcccctaca
     5041 gggactctgt gctcacctgg ctgctcaagg aaaatttggg ggggaactca cgcacagcca
     5101 tgattgcagc cctgagccct gctgacatca attacgagga gactctcagc accctcaggt
     5161 atgctgaccg caccaagcaa atccgctgca atgccatcat caacgaggac cctaatgccc
     5221 ggctgattag agagctgcag gaggaagtag cccggctgcg ggaactgctg atggctcagg
     5281 gactgtcagc ctctgctctg gaaggcctga agacggaaga agggagtgtc agaggcgccc
     5341 tgccagctgt gtcatctccc ccagctccag tttcaccctc atcacccacc acacataatg
     5401 gggagctgga gccgtcattc tcccccaaca cggagtccca gattgggcct gaggaagcca
     5461 tggagaggct gcaggagaca gagaagatta tagctgagct gaacgagaca tgggaggaga
     5521 agctacgcaa gacagaagcc ctgaggatgg agagagaagc attgctggct gagatggggg
     5581 tggccgtccg ggaggatggg ggaactgtgg gcgtcttctc tccaaagaag actccccacc
     5641 tggtgaacct gaacgaagac cctctgatgt ctgagtgtct gctctaccac atcaaagatg
     5701 gcgtcaccag ggtcggccaa gtagatatgg acatcaagct gaccggacag ttcattcggg
     5761 agcaacactg tctgttccgg agcatccccc agccagatgg agaagtggtg gtcactctgg
     5821 agccttgtga aggagctgag acatatgtga atgggaagct tgtgacggag ccgctggtgc
     5881 tgaagtcagg gaataggatt gtgatgggca agaaccacgt tttccgcttc aaccacccgg
     5941 agcaggcaag gctggaacgg gaacgagggg tccccccacc cccaggaccg ccctctgagc
     6001 cagtcgactg gaactttgcc cagaaggaac tgctggagca gcaaggcatc gacataaagc
     6061 tggaaatgga gaagaggctg caggatctgg agaatcagta ccggaaagaa aaggaagaag
     6121 ccgatcttct gctggagcag cagcgactgt atgcagactc ggacagcggg gatgactctg
     6181 acaagcgctc ttgtgaagag agctggaggc tcatctcctc cttgcgggag cagctgccgc
     6241 ccaccacggt ccagaccatt gtcaaacgct gtggtctgcc cagcagtggc aagcgcaggg
     6301 cccctcgcag ggtttatcag atcccccagc gacgcaggct gcagggcaaa gacccccgct
     6361 gggccaccat ggctgacctg aagatgcagg cggtgaagga gatctgctac gaggtggccc
     6421 tggctgactt ccgccacggg cgggctgaga ttgaggccct ggccgccctc aagatgcggg
     6481 agctgtgtcg cacctatggc aagccagacg gccccggaga cgcctggagg gctgtggccc
     6541 gggatgtctg ggacactgta ggcgaggagg aaggaggtgg agctggcagt ggtggtggca
     6601 gtgaggaggg agcccgaggg gcggaggtgg aggacctccg ggcccacatc gacaagctga
     6661 cggggattct gcaggaggtg aagctgcaga acagcagcaa ggaccgggag ctgcaggccc
     6721 tgcgggaccg catgctccgc atggagaggg tcatccccct ggcccaggat catgaggatg
     6781 agaatgaaga aggtggtgag gtcccctggg ccccgcctga aggatcagag gcagcagagg
     6841 aggcagcccc cagtgaccgc atgccgtcag cccggccccc ctcgccacca ctgtcaagct
     6901 gggagcgggt gtcacggctc atggaggagg accctgcctt ccgtcgtggt cgtcttcgct
     6961 ggctcaagca ggagcagcta cggctgcagg gactgcaggg ctctgggggc cggggcgggg
     7021 ggctgcgcag gcccccagcc cgctttgtgc cccctcacga ctgcaagcta cgcttcccct
     7081 tcaagagcaa cccccagcac cgggagtctt ggccagggat ggggagcggg gaggctccaa
     7141 ctccgctcca accccctgag gaggtcactc cccatccagc cacccctgcc cgccggcctc
     7201 cgagtccccg aaggtcccac catccccgca ggaactccct ggatggaggg ggccgatccc
     7261 ggggagcggg ttctgcacag cctgaacccc agcacttcca gcccaaaaag cacaactctt
     7321 atccccagcc accccaaccc tacccagccc agcggccccc agggccccgc taccccccat
     7381 acactactcc cccacgaatg agacggcagc gttctgcccc tgacctcaag gagagtgggg
     7441 cagctgtgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     7501 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     7561 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     7621 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     7681 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     7741 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     7801 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     7861 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     7921 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     7981 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     8041 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     8101 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     8161 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     8221 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     8281 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     8341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8401 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgatatcgcc
     8461 taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt
     8521 tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata
     8581 atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc gcccacagcg
     8641 gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca tgtgacaaac
     8701 cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc atctcttcgt
     8761 tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat ggcgaatgg
//
