LOCUS pET22b-mEB2-eGFP-6xHis_MBN.xdna 7083 bp DNA circular 20/10/2019 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments=pANNE 8193 = pET22b-mEB2-eGFP-6xHis Mungbean COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers CDS complement(140..157) /label=6His /SerialCloner_Color=&h008000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(1948..1967) /label=T7 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(4848..5476) /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin 6627..7067 /label=F1 ori /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(6617..7072) /label=M13 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_binding complement(1926..1948) /label=LacO /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(5628..6287) /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(176..889) /label=EGFP /SerialCloner_Color=&h32CD32 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(6527..6555) /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 2345..3436 /label=lacI /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(902..1879) /label=MAPRE2 /SerialCloner_Color=&hE7461B /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="opposite strand translation" \ /SerialCloner_Desc=translation="MPGPTQTLSPNGENNNDIIQDNGTIIPFRKHTVRGERSYSWGMAVNVYSTSITQETMSRHDIIAWVNDIVSLNYTKVEQLCSGAAYCQFMDMLFPGCISLKKVKFQAKLEHEYIHNFKLLQASFKRMNVDKVIPVEKLVKGRFQDNLDFIQWFKKFYDANYDGKEYDPVEARQGQDAIPPPDPGEQIFNLPKKSHHANSPTAGAAKS \ ORIGIN 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCGGACTTGT 181 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT 241 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT 301 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT 361 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT 421 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA 481 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT 541 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG 601 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT 661 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC 721 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG 781 CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT 841 CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGGaat 901 CGTACTCCTC CTGCTGCTGG GGCTGCTGGT CATGGGCTTG CTCCTCTGCT TCTGGCTCTT 961 CCGTTTGGCC CTCCTGTTCA TCGGAAGCGT AGAGCACTTC CATTAGTCGC TGCACGAGGT 1021 CATCGTTCTC CTGCCCATGT TCTTGGCACA GCAGCTCAAT CTCTCTCAAC TTCCCGAAGT 1081 AGAAATCCCT CTCCTTCTCC ACACCTTCTA GGGCGAGTTT CAATGAATGT ACCTGCTCGT 1141 TGAGCTGTAT GACCTGCGTT TCTAAATCTT TGTCAGATCT GGATGCAGAG CCACTGGATG 1201 AAGCCCTTTT GGCAGACGAG GGGCGAGAAG GTGTGGATCC TGGCTTAGAT GCAGGACTTG 1261 ACTTAGCCGC GCCTGCTGTG GGGGAGTTTG CATGGTGCGA CTTCTTGGGC AGGTTGAAGA 1321 TCTGTTCACC AGGGTCAGGG GGAGGAATTG CATCTTGCCC TTGTCGGGCC TCAACAGGAT 1381 CGTACTCCTT CCCGTCATAG TTAGCATCGT AGAACTTCTT AAACCACTGA ATAAAATCCA 1441 GGTTGTCTTG GAAACGCCCT TTCACCAGCT TCTCCACTGG GATTACCTTA TCAACATTCA 1501 TCCGTTTAAA GGATGCTTGC AGAAGTTTAA AGTTGTGAAT ATACTCATGC TCCAACTTTG 1561 CTTGGAATTT TACTTTCTTC AAACTAATAC AGCCCGGGAA GAGCATGTCC ATGAACTGGC 1621 AATAGGCTGC TCCTGAACAG AGCTGTTCCA CTTTTGTGTA GTTTAGAGAC ACGATGTCAT 1681 TGACCCATGC AATGATGTCA TGCCTGCTCA TAGTCTCCTG GGTTATCGAG GTAGAATACA 1741 CATTGACCGC CATTCCCCAA CTGTAGGACC GCTCCCCGCG CACTGTGTGC TTCCGGAAAG 1801 GAATGATAGT CCCGTTATCC TGGATGATGT CGTTGTTGTT CTCGCCATTT GGGGACAACG 1861 TTTGGGTCGG CCCGGGCATA TGTATATCTC CTTCTTAAAG TTAAACAAAA TTATTTCTAG 1921 AGGGGAATTG TTATCCGCTC ACAATTCCCC TATAGTGAGT CGTATTAATT TCGCGGGATC 1981 GAGATCTCGA TCCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC CACAGGTGCG 2041 GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG ATCGGGCTCG CCACTTCGGG 2101 CTCATGAGCG CTTGTTTCGG CGTGGGTATG GTGGCAGGCC CCGTGGCCGG GGGACTGTTG 2161 GGCGCCATCT CCTTGCATGC ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA 2221 CTACTGGGCT GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGTCGAGA TCCCGGACAC 2281 CATCGAATGG CGCAAAACCT TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT 2341 CAGGGTGGTG AATGTGAAAC CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC 2401 TTATCAGACC GTTTCCCGCG TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA 2461 AAAAGTGGAA GCGGCGATGG CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT 2521 GGCGGGCAAA CAGTCGTTGC TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC 2581 GTCGCAAATT GTCGCGGCGA TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT 2641 GTCGATGGTA GAACGAAGCG GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC 2701 GCAACGCGTC AGTGGGCTGA TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTGCTGT 2761 GGAAGCTGCC TGCACTAATG TTCCGGCGTT ATTTCTTGAT GTCTCTGACC AGACACCCAT 2821 CAACAGTATT ATTTTCTCCC ATGAAGACGG TACGCGACTG GGCGTGGAGC ATCTGGTCGC 2881 ATTGGGTCAC CAGCAAATCG CGCTGTTAGC GGGCCCATTA AGTTCTGTCT CGGCGCGTCT 2941 GCGTCTGGCT GGCTGGCATA AATATCTCAC TCGCAATCAA ATTCAGCCGA TAGCGGAACG 3001 GGAAGGCGAC TGGAGTGCCA TGTCCGGTTT TCAACAAACC ATGCAAATGC TGAATGAGGG 3061 CATCGTTCCC ACTGCGATGC TGGTTGCCAA CGATCAGATG GCGCTGGGCG CAATGCGCGC 3121 CATTACCGAG TCCGGGCTGC GCGTTGGTGC GGATATCTCG GTAGTGGGAT ACGACGATAC 3181 CGAAGACAGC TCATGTTATA TCCCGCCGTT AACCACCATC AAACAGGATT TTCGCCTGCT 3241 GGGGCAAACC AGCGTGGACC GCTTGCTGCA ACTCTCTCAG GGCCAGGCGG TGAAGGGCAA 3301 TCAGCTGTTG CCCGTCTCAC TGGTGAAAAG AAAAACCACC CTGGCGCCCA ATACGCAAAC 3361 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 3421 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTAAGTTA GCTCACTCAT TAGGCACCGG 3481 GATCTCGACC GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG 3541 GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG 3601 TGCCGGCAGC GCTCTGGGTC ATTTTCGGCG AGGACCGCTT TCGCTGGAGC GCGACGATGA 3661 TCGGCCTGTC GCTTGCGGTA TTCGGAATCT TGCACGCCCT CGCTCAAGCC TTCGTCACTG 3721 GTCCCGCCAC CAAACGTTTC GGCGAGAAGC AGGCCATTAT CGCCGGCATG GCGGCCCCAC 3781 GGGTGCGCAT GATCGTGCTC CTGTCGTTGA GGACCCGGCT AGGCTGGCGG GGTTGCCTTA 3841 CTGGTTAGCA GAATGAATCA CCGATACGCG AGCGAACGTG AAGCGACTGC TGCTGCAAAA 3901 CGTCTGCGAC CTGAGCAACA ACATGAATGG TCTTCGGTTT CCGTGTTTCG TAAAGTCTGG 3961 AAACGCGGAA GTCAGCGCCC TGCACCATTA TGTTCCGGAT CTGCATCGCA GGATGCTGCT 4021 GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCG CTGGCATTGA CCCTGAGTGA 4081 TTTTTCTCTG GTCCCGCCGC ATCCATACCG CCAGTTGTTT ACCCTCACAA CGTTCCAGTA 4141 ACCGGGCATG TTCATCATCA GTAACCCGTA TCGTGAGCAT CCTCTCTCGT TTCATCGGTA 4201 TCATTACCCC CATGAACAGA AATCCCCCTT ACACGGAGGC ATCAGTGACC AAACAGGAAA 4261 AAACCGCCCT TAACATGGCC CGCTTTATCA GAAGCCAGAC ATTAACGCTT CTGGAGAAAC 4321 TCAACGAGCT GGACGCGGAT GAACAGGCAG ACATCTGTGA ATCGCTTCAC GACCACGCTG 4381 ATGAGCTTTA CCGCAGCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 4441 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 4501 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA 4561 GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 4621 GCACCATATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG 4681 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 4741 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 4801 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 4861 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 4921 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 4981 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 5041 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 5101 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 5161 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 5221 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 5281 TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 5341 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 5401 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 5461 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 5521 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 5581 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 5641 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 5701 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 5761 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 5821 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 5881 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 5941 GCAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 6001 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 6061 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 6121 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 6181 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 6241 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 6301 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 6361 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 6421 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 6481 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 6541 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 6601 CGAAAAGTGC CACCTGAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT 6661 TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA 6721 AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA 6781 AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA 6841 CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG 6901 AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA 6961 AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG 7021 CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCCATTCG 7081 CCA //