LOCUS       pET22b-mEB3-eGFP-6xHis_MBN.xdna    6948 bp      DNA     circular   19/10/2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=pANNE 8159 = pET22b-mEB3-eGFP-6xHis Mungbean
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     complement(1813..1832)
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4713..5341)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      6492..6932
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(6482..6937)
                     /label=M13 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(1791..1813)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5493..6152)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(176..889)
                     /label=EGFP
                     /SerialCloner_Color=&h32CD32
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6392..6420)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2210..3301
                     /label=lacI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(140..157)
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    complement(902..1744)
                     /label=Mapre3
                     /SerialCloner_Color=&hE7461B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="opposite strand translation" \ 
                     /SerialCloner_Desc=translation="MAVNVYSTSVTSENLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYCQFMDMLFPGCVHLRKVKFQAKLEHEYIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDNFEFIQWFKKFFDANYDGKDYNPLLARQGQDVAPPPNPGDQIFNKSKKLIGTAVPQRTSPTGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHEADAQILELNQ \ 
ORIGIN
        1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 
       61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 
      121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCGGACTTGT 
      181 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT 
      241 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT 
      301 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT 
      361 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT 
      421 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA 
      481 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT 
      541 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG 
      601 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT 
      661 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC 
      721 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG 
      781 CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT 
      841 CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGGaat 
      901 CGTACTCGTC CTGGTCTTCC TGTTGGTGTT CTTCAATCTC ATCATCCTCA GGGGGTGCAA 
      961 ATCCCTCCTC CGTGGCATAG AGAATGCCAA TGATGCCCGA GATGACGGGG CTGTTCTCGC 
     1021 TCTCATGTTC CTGGCAGATC AGCTCGATGT CTCGCAATTT GCTGAAATAG AAATCTCGTT 
     1081 CTTTCTCAAG CCCGTCTACG GTCAGCTTCA AGTCCAGCAG CTGCTGGTTA AGCTCGAGGA 
     1141 TCTGGGCGTC AGCCTCATGG CCACCGTTTC GGGCTGAGGG GGGATTCTTC CGGAGGATGC 
     1201 AGGGCGGAGC CACGTTGCTG AGTCGTCCAG AGGTCTGCAT GTTCTTGGGG CCTGTGGGGG 
     1261 ACGTCCTCTG CGGAACTGCT GTGCCAATGA GTTTCTTGGA TTTGTTGAAG ATCTGATCAC 
     1321 CTGGGTTAGG AGGTGGTGCT ACGTCCTGGC CCTGCCGCGC CAGCAGAGGG TTGTAATCCT 
     1381 TTCCATCATA GTTTGCGTCA AAGAATTTCT TAAACCACTG TATAAACTCA AAATTATCTT 
     1441 GGAATTTTCC TTTCACTAAC TTCTCTACGG GAATGATTTT GTCAACACCC ATCTTCTTGA 
     1501 AAGCTGCTTG CAGCACCTTG AAGTTGTGGA TGTACTCGTG TTCTAGTTTG GCCTGGAACT 
     1561 TGACCTTCCT CAAGTGAACA CAGCCAGGGA AGAGCATGTC CATGAACTGG CAGTAGGCTG 
     1621 CCCCTGAACA GAGCTGTTCA ATCTTGGTAT AATTGAGGTG CAGAGAGTCA TTGACCCATG 
     1681 CAAGCATATC ATGGCGACTC AGATTCTCAC TGGTCACAGA AGTGGAGTAC ACATTGACAG 
     1741 CCATATGTAT ATCTCCTTCT TAAAGTTAAA CAAAATTATT TCTAGAGGGG AATTGTTATC 
     1801 CGCTCACAAT TCCCCTATAG TGAGTCGTAT TAATTTCGCG GGATCGAGAT CTCGATCCTC 
     1861 TACGCCGGAC GCATCGTGGC CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT 
     1921 ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT 
     1981 TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG 
     2041 CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC 
     2101 CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGAGATCCCG GACACCATCG AATGGCGCAA 
     2161 AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG TGGTGAATGT 
     2221 GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC 
     2281 CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC 
     2341 GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG GCAAACAGTC 
     2401 GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC AAATTGTCGC 
     2461 GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA TGGTAGAACG 
     2521 AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG 
     2581 GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC 
     2641 TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA GTATTATTTT 
     2701 CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG GTCACCAGCA 
     2761 AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG 
     2821 GCATAAATAT CTCACTCGCA ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG 
     2881 TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC 
     2941 GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG 
     3001 GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC GATACCGAAG ACAGCTCATG 
     3061 TTATATCCCG CCGTTAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT 
     3121 GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT 
     3181 CTCACTGGTG AAAAGAAAAA CCACCCTGGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 
     3241 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 
     3301 AGCGCAACGC AATTAATGTA AGTTAGCTCA CTCATTAGGC ACCGGGATCT CGACCGATGC 
     3361 CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG 
     3421 CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT 
     3481 GGGTCATTTT CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC CTGTCGCTTG 
     3541 CGGTATTCGG AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC GCCACCAAAC 
     3601 GTTTCGGCGA GAAGCAGGCC ATTATCGCCG GCATGGCGGC CCCACGGGTG CGCATGATCG 
     3661 TGCTCCTGTC GTTGAGGACC CGGCTAGGCT GGCGGGGTTG CCTTACTGGT TAGCAGAATG 
     3721 AATCACCGAT ACGCGAGCGA ACGTGAAGCG ACTGCTGCTG CAAAACGTCT GCGACCTGAG 
     3781 CAACAACATG AATGGTCTTC GGTTTCCGTG TTTCGTAAAG TCTGGAAACG CGGAAGTCAG 
     3841 CGCCCTGCAC CATTATGTTC CGGATCTGCA TCGCAGGATG CTGCTGGCTA CCCTGTGGAA 
     3901 CACCTACATC TGTATTAACG AAGCGCTGGC ATTGACCCTG AGTGATTTTT CTCTGGTCCC 
     3961 GCCGCATCCA TACCGCCAGT TGTTTACCCT CACAACGTTC CAGTAACCGG GCATGTTCAT 
     4021 CATCAGTAAC CCGTATCGTG AGCATCCTCT CTCGTTTCAT CGGTATCATT ACCCCCATGA 
     4081 ACAGAAATCC CCCTTACACG GAGGCATCAG TGACCAAACA GGAAAAAACC GCCCTTAACA 
     4141 TGGCCCGCTT TATCAGAAGC CAGACATTAA CGCTTCTGGA GAAACTCAAC GAGCTGGACG 
     4201 CGGATGAACA GGCAGACATC TGTGAATCGC TTCACGACCA CGCTGATGAG CTTTACCGCA 
     4261 GCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 
     4321 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 
     4381 CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT 
     4441 ATACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATATGCGG 
     4501 TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC TTCCGCTTCC 
     4561 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA 
     4621 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA 
     4681 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG 
     4741 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 
     4801 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT 
     4861 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT 
     4921 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 
     4981 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT 
     5041 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 
     5101 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC 
     5161 TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA 
     5221 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT 
     5281 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT 
     5341 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 
     5401 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 
     5461 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 
     5521 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 
     5581 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC 
     5641 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 
     5701 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 
     5761 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTGCAGG CATCGTGGTG 
     5821 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 
     5881 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 
     5941 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 
     6001 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 
     6061 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 
     6121 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 
     6181 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 
     6241 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 
     6301 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 
     6361 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6421 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6481 GAAATTGTAA ACGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA 
     6541 TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG 
     6601 ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC 
     6661 AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC 
     6721 TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC 
     6781 CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA 
     6841 GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC 
     6901 ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCCC ATTCGCCA
//