LOCUS       pET22b-mEB1-mCherry-6xHis.xdna    6915 bp      DNA     circular   19/10/2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=pANNE xxxx = pET22b-mEB1-mCherry-6xHis
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             complement(140..157)
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(1780..1799)
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4680..5308)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      6459..6899
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(6449..6904)
                     /label=M13 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(1758..1780)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5460..6119)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(176..880)
                     /label=mCherry
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(6359..6387)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2177..3268
                     /label=lacI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(902..1711)
                     /label=Mapre1
                     /SerialCloner_Color=&hE7461B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 
       61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 
      121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCGGACTTGT 
      181 ACAGCTCGTC CATGCCGCCG GTGGAGTGGC GGCCCTCGGC GCGTTCGTAC TGTTCCACGA 
      241 TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAACTTGAT GTTGACGTTG TAGGCGCCGG 
      301 GCAGCTGCAC GGGCTTCTTG GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC 
      361 CGTCCTTCAG CTTCAGCCTC TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA 
      421 TCCGCTCGGA GGAGGCCTCC CAGCCCATGG TCTTCTTCTG CATTACGGGG CCGTCGGAGG 
      481 GGAAGTTGGT GCCGCGCAGC TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT 
      541 CCTGGGTCAC GGTCACCACG CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CACTTGAAGC 
      601 CCTCGGGGAA GGACAGCTTC AAGTAGTCGG GGATGTCGGC GGGGTGCTTC ACGTAGGCCT 
      661 TGGAGCCGTA CATGAACTGA GGGGACAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT 
      721 TGGTCACCTT CAGCTTGGCG GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG CCCTCGCCCT 
      781 CGATCTCGAA CTCGTGGCCG TTCACGGAGC CCTCCATGTG CACCTTGAAG CGCATGAACT 
      841 CCTTGATGAT GGCCATGTTA TCCTCCTCGC CCTTGCTCAC CATggtggcg ggatcccggg 
      901 cCCGCGGATA CTCTTCTTGT TCCTCCTGTG GGCCCCCTTC ATCAGGTATC ACAAAGCCTT 
      961 CATCTGTGGC ATAAAGAATA TCTACAATCC TCTGCAGTAC AGGGTCGTTT TCCCCCTCGT 
     1021 TCTCCTGGCA AATCAGTTCA ATGTTCCTTA GCTTTCCGAA GTAGAAGTCT CTCTCCTTCT 
     1081 CCAAGTCTTC AACAGTAAGC TTCAGTACTT TGACCTGCTG CATCAATTCA GCTGCTTCAT 
     1141 CATCTCCATT GCCCACACCA GGATTCTTTC GCACCATTCC GGGGCCAGCC TTAGGAGCTG 
     1201 CAGTAGTCCT CTGTGTTGCA ATGGGTCTCT GTGGGGCTGC AGTACTGGAG CCGAGAGGTT 
     1261 TCTTCGGTTT ACTCAAAGCT GGGGCGACAA GAGAAGGAGC CACTGCAGTT TCTTGACCTT 
     1321 GTCTGGCAGC TACAGGATCA TACTCTTTTC CATCATAATT TGCATCAAAA AACTTCTTGA 
     1381 ACCATTGAAC AAATTCAAAA TTGTCCTGAA ATTTTCCTTT TACTAATTTA TCCACAGGAA 
     1441 TTATTTTGTC AACGCCCATC CTCTTGAAGC CTGCTTGTAG TATTTTGAAG TTCTGGATAT 
     1501 ATTCATGTTC TAGCTTAGCT TGGAATTTCA CTTTCTTCAA GGCAATGGAT CCAGGGAAGA 
     1561 GCATGTCCAT AAACTGACAA TATGCAGCCC CTGAACACAA CTGTTCTATC TTTGTCAGAT 
     1621 TCAACTGCAG AGATTCATTG ATCCAAGCCA GCATGTCATG TCGACTTAGG TTATCACTGG 
     1681 TGACTGACGT AGAGTACACA TTCACTGCCA TATGTATATC TCCTTCTTAA AGTTAAACAA 
     1741 AATTATTTCT AGAGGGGAAT TGTTATCCGC TCACAATTCC CCTATAGTGA GTCGTATTAA 
     1801 TTTCGCGGGA TCGAGATCTC GATCCTCTAC GCCGGACGCA TCGTGGCCGG CATCACCGGC 
     1861 GCCACAGGTG CGGTTGCTGG CGCCTATATC GCCGACATCA CCGATGGGGA AGATCGGGCT 
     1921 CGCCACTTCG GGCTCATGAG CGCTTGTTTC GGCGTGGGTA TGGTGGCAGG CCCCGTGGCC 
     1981 GGGGGACTGT TGGGCGCCAT CTCCTTGCAT GCACCATTCC TTGCGGCGGC GGTGCTCAAC 
     2041 GGCCTCAACC TACTACTGGG CTGCTTCCTA ATGCAGGAGT CGCATAAGGG AGAGCGTCGA 
     2101 GATCCCGGAC ACCATCGAAT GGCGCAAAAC CTTTCGCGGT ATGGCATGAT AGCGCCCGGA 
     2161 AGAGAGTCAA TTCAGGGTGG TGAATGTGAA ACCAGTAACG TTATACGATG TCGCAGAGTA 
     2221 TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC 
     2281 GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG AATTACATTC CCAACCGCGT 
     2341 GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC GTTGCCACCT CCAGTCTGGC 
     2401 CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC 
     2461 CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA 
     2521 CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC TATCCGCTGG ATGACCAGGA 
     2581 TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG TTATTTCTTG ATGTCTCTGA 
     2641 CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC GGTACGCGAC TGGGCGTGGA 
     2701 GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT 
     2761 CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC ACTCGCAATC AAATTCAGCC 
     2821 GATAGCGGAA CGGGAAGGCG ACTGGAGTGC CATGTCCGGT TTTCAACAAA CCATGCAAAT 
     2881 GCTGAATGAG GGCATCGTTC CCACTGCGAT GCTGGTTGCC AACGATCAGA TGGCGCTGGG 
     2941 CGCAATGCGC GCCATTACCG AGTCCGGGCT GCGCGTTGGT GCGGATATCT CGGTAGTGGG 
     3001 ATACGACGAT ACCGAAGACA GCTCATGTTA TATCCCGCCG TTAACCACCA TCAAACAGGA 
     3061 TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC AGGGCCAGGC 
     3121 GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGGTGAAA AGAAAAACCA CCCTGGCGCC 
     3181 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA 
     3241 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTAAGT TAGCTCACTC 
     3301 ATTAGGCACC GGGATCTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC 
     3361 GGTGGGCGCG GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC 
     3421 TCGTAGGACA GGTGCCGGCA GCGCTCTGGG TCATTTTCGG CGAGGACCGC TTTCGCTGGA 
     3481 GCGCGACGAT GATCGGCCTG TCGCTTGCGG TATTCGGAAT CTTGCACGCC CTCGCTCAAG 
     3541 CCTTCGTCAC TGGTCCCGCC ACCAAACGTT TCGGCGAGAA GCAGGCCATT ATCGCCGGCA 
     3601 TGGCGGCCCC ACGGGTGCGC ATGATCGTGC TCCTGTCGTT GAGGACCCGG CTAGGCTGGC 
     3661 GGGGTTGCCT TACTGGTTAG CAGAATGAAT CACCGATACG CGAGCGAACG TGAAGCGACT 
     3721 GCTGCTGCAA AACGTCTGCG ACCTGAGCAA CAACATGAAT GGTCTTCGGT TTCCGTGTTT 
     3781 CGTAAAGTCT GGAAACGCGG AAGTCAGCGC CCTGCACCAT TATGTTCCGG ATCTGCATCG 
     3841 CAGGATGCTG CTGGCTACCC TGTGGAACAC CTACATCTGT ATTAACGAAG CGCTGGCATT 
     3901 GACCCTGAGT GATTTTTCTC TGGTCCCGCC GCATCCATAC CGCCAGTTGT TTACCCTCAC 
     3961 AACGTTCCAG TAACCGGGCA TGTTCATCAT CAGTAACCCG TATCGTGAGC ATCCTCTCTC 
     4021 GTTTCATCGG TATCATTACC CCCATGAACA GAAATCCCCC TTACACGGAG GCATCAGTGA 
     4081 CCAAACAGGA AAAAACCGCC CTTAACATGG CCCGCTTTAT CAGAAGCCAG ACATTAACGC 
     4141 TTCTGGAGAA ACTCAACGAG CTGGACGCGG ATGAACAGGC AGACATCTGT GAATCGCTTC 
     4201 ACGACCACGC TGATGAGCTT TACCGCAGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA 
     4261 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA 
     4321 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA 
     4381 CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT 
     4441 TGTACTGAGA GTGCACCATA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA 
     4501 TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 
     4561 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 
     4621 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 
     4681 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 
     4741 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 
     4801 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 
     4861 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 
     4921 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 
     4981 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 
     5041 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 
     5101 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 
     5161 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 
     5221 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 
     5281 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 
     5341 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 
     5401 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 
     5461 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 
     5521 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 
     5581 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 
     5641 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 
     5701 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 
     5761 GTTGCCATTG CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC 
     5821 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 
     5881 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 
     5941 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 
     6001 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 
     6061 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC 
     6121 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 
     6181 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 
     6241 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 
     6301 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6361 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6421 CGCACATTTC CCCGAAAAGT GCCACCTGAA ATTGTAAACG TTAATATTTT GTTAAAATTC 
     6481 GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC 
     6541 CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG 
     6601 AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC 
     6661 GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA 
     6721 GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG 
     6781 AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT 
     6841 GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC 
     6901 GCGTCCCATT CGCCA
//