LOCUS       pET22b-mEB3-mCherry-6xHis.xdna    6954 bp      DNA     circular   19/10/2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=pANNE xxxx = pET22b-mEB3-mCherry-6xHis
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             complement(140..157)
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(1819..1838)
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4719..5347)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      6498..6938
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(6488..6943)
                     /label=M13 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(1797..1819)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5499..6158)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(176..880)
                     /label=mCherry
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6398..6426)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2216..3307
                     /label=lacI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    complement(902..1750)
                     /label=MAPRE3
                     /SerialCloner_Color=&hE7461B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 
       61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 
      121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCGGACTTGT 
      181 ACAGCTCGTC CATGCCGCCG GTGGAGTGGC GGCCCTCGGC GCGTTCGTAC TGTTCCACGA 
      241 TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAACTTGAT GTTGACGTTG TAGGCGCCGG 
      301 GCAGCTGCAC GGGCTTCTTG GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC 
      361 CGTCCTTCAG CTTCAGCCTC TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA 
      421 TCCGCTCGGA GGAGGCCTCC CAGCCCATGG TCTTCTTCTG CATTACGGGG CCGTCGGAGG 
      481 GGAAGTTGGT GCCGCGCAGC TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT 
      541 CCTGGGTCAC GGTCACCACG CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CACTTGAAGC 
      601 CCTCGGGGAA GGACAGCTTC AAGTAGTCGG GGATGTCGGC GGGGTGCTTC ACGTAGGCCT 
      661 TGGAGCCGTA CATGAACTGA GGGGACAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT 
      721 TGGTCACCTT CAGCTTGGCG GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG CCCTCGCCCT 
      781 CGATCTCGAA CTCGTGGCCG TTCACGGAGC CCTCCATGTG CACCTTGAAG CGCATGAACT 
      841 CCTTGATGAT GGCCATGTTA TCCTCCTCGC CCTTGCTCAC CATggtggcg ggatcccggg 
      901 cCCGCGGGTA CTCGTCCTGG TCTTCCTGTT GGTGTTCTTC AATCTCATCA TCCTCAGGGG 
      961 GTGCAAATCC CTCCTCCGTG GCATAGAGAA TGCCAATGAT GCCCGAGATG ACGGGGCTGT 
     1021 TCTCGCTCTC ATGTTCCTGG CAGATCAGCT CGATGTCTCG CAATTTGCTG AAATAGAAAT 
     1081 CTCGTTCTTT CTCAAGCCCG TCTACGGTCA GCTTCAAGTC CAGCAGCTGC TGGTTAAGCT 
     1141 CGAGGATCTG GGCGTCAGCC TCATGGCCAC CGTTTCGGGC TGAGGGGGGA TTCTTCCGGA 
     1201 GGATGCAGGG CGGAGCCACG TTGCTGAGTC GTCCAGAGGT CTGCATGTTC TTGGGGCCTG 
     1261 TGGGGGACGT CCTCTGCGGA ACTGCTGTGC CAATGAGTTT CTTGGATTTG TTGAAGATCT 
     1321 GATCACCTGG GTTAGGAGGT GGTGCTACGT CCTGGCCCTG CCGCGCCAGC AGAGGGTTGT 
     1381 AATCCTTTCC ATCATAGTTT GCGTCAAAGA ATTTCTTAAA CCACTGTATA AACTCAAAAT 
     1441 TATCTTGGAA TTTTCCTTTC ACTAACTTCT CTACGGGAAT GATTTTGTCA ACACCCATCT 
     1501 TCTTGAAAGC TGCTTGCAGC ACCTTGAAGT TGTGGATGTA CTCGTGTTCT AGTTTGGCCT 
     1561 GGAACTTGAC CTTCCTCAAG TGAACACAGC CAGGGAAGAG CATGTCCATG AACTGGCAGT 
     1621 AGGCTGCCCC TGAACAGAGC TGTTCAATCT TGGTATAATT GAGGTGCAGA GAGTCATTGA 
     1681 CCCATGCAAG CATATCATGG CGACTCAGAT TCTCACTGGT CACAGAAGTG GAGTACACAT 
     1741 TGACAGCCAT ATGTATATCT CCTTCTTAAA GTTAAACAAA ATTATTTCTA GAGGGGAATT 
     1801 GTTATCCGCT CACAATTCCC CTATAGTGAG TCGTATTAAT TTCGCGGGAT CGAGATCTCG 
     1861 ATCCTCTACG CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC GGTTGCTGGC 
     1921 GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG GCTCATGAGC 
     1981 GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT GGGCGCCATC 
     2041 TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC 
     2101 TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAG ATCCCGGACA CCATCGAATG 
     2161 GCGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT 
     2221 GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC 
     2281 CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA 
     2341 AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA 
     2401 ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT 
     2461 TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT 
     2521 AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT 
     2581 CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC 
     2641 CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT 
     2701 TATTTTCTCC CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA 
     2761 CCAGCAAATC GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC 
     2821 TGGCTGGCAT AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA 
     2881 CTGGAGTGCC ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC 
     2941 CACTGCGATG CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA 
     3001 GTCCGGGCTG CGCGTTGGTG CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG 
     3061 CTCATGTTAT ATCCCGCCGT TAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC 
     3121 CAGCGTGGAC CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT 
     3181 GCCCGTCTCA CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC 
     3241 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG 
     3301 GCAGTGAGCG CAACGCAATT AATGTAAGTT AGCTCACTCA TTAGGCACCG GGATCTCGAC 
     3361 CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA 
     3421 TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG 
     3481 CGCTCTGGGT CATTTTCGGC GAGGACCGCT TTCGCTGGAG CGCGACGATG ATCGGCCTGT 
     3541 CGCTTGCGGT ATTCGGAATC TTGCACGCCC TCGCTCAAGC CTTCGTCACT GGTCCCGCCA 
     3601 CCAAACGTTT CGGCGAGAAG CAGGCCATTA TCGCCGGCAT GGCGGCCCCA CGGGTGCGCA 
     3661 TGATCGTGCT CCTGTCGTTG AGGACCCGGC TAGGCTGGCG GGGTTGCCTT ACTGGTTAGC 
     3721 AGAATGAATC ACCGATACGC GAGCGAACGT GAAGCGACTG CTGCTGCAAA ACGTCTGCGA 
     3781 CCTGAGCAAC AACATGAATG GTCTTCGGTT TCCGTGTTTC GTAAAGTCTG GAAACGCGGA 
     3841 AGTCAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC AGGATGCTGC TGGCTACCCT 
     3901 GTGGAACACC TACATCTGTA TTAACGAAGC GCTGGCATTG ACCCTGAGTG ATTTTTCTCT 
     3961 GGTCCCGCCG CATCCATACC GCCAGTTGTT TACCCTCACA ACGTTCCAGT AACCGGGCAT 
     4021 GTTCATCATC AGTAACCCGT ATCGTGAGCA TCCTCTCTCG TTTCATCGGT ATCATTACCC 
     4081 CCATGAACAG AAATCCCCCT TACACGGAGG CATCAGTGAC CAAACAGGAA AAAACCGCCC 
     4141 TTAACATGGC CCGCTTTATC AGAAGCCAGA CATTAACGCT TCTGGAGAAA CTCAACGAGC 
     4201 TGGACGCGGA TGAACAGGCA GACATCTGTG AATCGCTTCA CGACCACGCT GATGAGCTTT 
     4261 ACCGCAGCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 
     4321 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 
     4381 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG 
     4441 GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 
     4501 ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC 
     4561 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT 
     4621 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG 
     4681 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC 
     4741 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 
     4801 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT 
     4861 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG 
     4921 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG 
     4981 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT 
     5041 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 
     5101 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC 
     5161 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC 
     5221 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT 
     5281 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC 
     5341 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 
     5401 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 
     5461 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA 
     5521 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG 
     5581 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC 
     5641 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 
     5701 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT 
     5761 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TGCAGGCATC 
     5821 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG 
     5881 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC 
     5941 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT 
     6001 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG 
     6061 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT 
     6121 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG 
     6181 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA 
     6241 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA 
     6301 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC 
     6361 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6421 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6481 CCACCTGAAA TTGTAAACGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC 
     6541 AGCTCATTTT TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG 
     6601 ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG 
     6661 GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA 
     6721 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA 
     6781 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG 
     6841 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA 
     6901 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCA
//