LOCUS       pAAV-hSyn-GCaMP6m-p2A-ChRmine-Kv2.1-WPRE        7097 bp    DNA     circular     19-JUL-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-12908662|
COMMENT     VNTDBDATE|-12908662|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-hSyn-GCaMP6m-p2A-ChRmine-Kv2.1-WPRE|
COMMENT     VNTAUTHORNAME|Charu|
COMMENT     VNTAUTHOREML|charur@stanford.edu|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV-hSyn-GCaMP6m-p2A-ChRmine-Kv2.1-WPRE" 0 7097 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI" "AarI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AloI" 
COMMENT         "AluI" "AlwI" "AlwNI" "ApaBI" "ApaI" "ApoI" "AscI" "AseI" "AsiSI" 
COMMENT         "AvaII" "AvrII" "BaeI" "BanI" "BanII" "BbeI" "Bbr7I" "BbsI" "BbvCI" 
COMMENT         "BbvI" "BccI" "BceAI" "BcefI" "BcgI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" "BmrI" "BmtI" "BplI" "BpmI" 
COMMENT         "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" "BsaJI" "BsaWI" "BsaXI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI" 
COMMENT         "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" "Bsp1286I" "Bsp24I" "BspCNI" 
COMMENT         "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" "BsrGI" "BsrI" "BssHII" 
COMMENT         "BssSI" "BstAPI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstXI" "BstYI" "BstZ17I" "Bsu36I" "BtgI" "BthCI" "BtsI" "Cac8I" "CdiI" 
COMMENT         "ChaI" "CjeI" "CjePI" "Csp6I" "CviAII" "CviJI" "DdeI" "DpnI" "DraI" 
COMMENT         "DraIII" "DrdI" "EaeI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" 
COMMENT         "EcoNI" "EcoO109I" "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" 
COMMENT         "HaeIV" "HgaI" "HhaI" "Hin4I" "HincII" "HinfI" "HinP1I" "HpaI" "HpaII" 
COMMENT         "HphI" "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4III" "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" 
COMMENT         "MaeIII" "MboI" "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" 
COMMENT         "MseI" "MslI" "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NdeI" "NgoMIV" 
COMMENT         "NheI" "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" 
COMMENT         "PciI" "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" 
COMMENT         "PshAI" "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" "Sse232I" 
COMMENT         "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" "StyI" 
COMMENT         "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" "Tsp45I" 
COMMENT         "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" "UnbI" "UthSI" 
COMMENT         "VpaK11AI" "XbaI" "XcmI" "XhoI" "XmnI" "ZraI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 7097 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 3434 0 5036 0 5533 0 
COMMENT            6779 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 9 41 0 52 0 732 0 2140 0 
COMMENT            3831 0 4136 0 4446 0 4457 0 4913 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 648 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 3 1852 0 3220 0 3815 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3201 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 3213 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 658 0 1978 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 7 0 170 0 1609 0 4499 0 0 
COMMENT            "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 4448 0 4459 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 4446 0 4457 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatII" "gacgtc" 6 0 3 434 0 3678 0 5286 0 0 
COMMENT             "") 
COMMENT        #12=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 2 642 0 1011 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AccI" "gtmkac" 3 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AceIII" "cagctc" 14 18 14 834 0 984 1 1032 1 
COMMENT             1065 1 1386 1 1542 1 1590 1 2787 1 2844 1 3021 1 3501 0 4962 1 5159 
COMMENT             1 5901 1 0 "") 
COMMENT        #15=(CRSite (CStringList) "AciI" "ccgc" 2 4 83 38 0 143 1 147 0 217 1 
COMMENT             328 1 362 0 364 1 383 0 390 1 403 0 406 0 449 0 484 0 489 0 492 0 
COMMENT             509 0 561 1 593 1 603 1 652 0 794 0 814 1 967 0 970 0 1009 1 1244 0 
COMMENT             1285 0 1352 0 1391 0 1571 1 2894 1 2907 1 2911 0 3012 0 3076 0 3104 
COMMENT             1 3534 1 3547 0 3560 0 3670 1 3711 1 3725 0 3727 1 3746 1 3756 0 
COMMENT             3802 0 3810 0 4038 1 4098 0 4344 1 4353 1 4357 0 4462 1 4512 1 4538 
COMMENT             1 4551 0 4588 1 4602 1 4605 1 4633 0 4660 0 5065 0 5091 0 5101 0 
COMMENT             5140 0 5314 1 5361 0 5460 1 5569 1 5646 1 5690 0 5811 1 5857 0 6048 
COMMENT             1 6139 1 6501 0 6510 1 6645 0 6755 1 6876 1 6895 1 7022 1 7050 1 0 
COMMENT             "") 
COMMENT        #16=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 5019 0 5603 0 5976 0 0 
COMMENT             "") 
COMMENT        #17=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 10 339 1 438 0 1179 0 
COMMENT             1223 1 1422 0 1498 0 2634 0 3175 0 5539 0 6551 1 0 "") 
COMMENT        #18=(CRSite (CStringList) "AfeI" "agcgct" 4 0 2 579 0 3826 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 3196 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflIII" "acrygt" 2 0 2 151 0 7093 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 4 71 0 3958 0 4432 0 
COMMENT             6205 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 6 154 0 660 0 1056 0 
COMMENT             1206 0 1383 0 3902 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "AloI" "gaacnnnnnntcc" 26 21 2 4320 0 4845 1 0 
COMMENT             "") 
COMMENT        #25=(CRSite (CStringList) "AluI" "agct" 3 0 43 12 0 353 0 711 0 727 0 
COMMENT             824 0 998 0 1046 0 1079 0 1151 0 1184 0 1400 0 1448 0 1556 0 1604 0 
COMMENT             1693 0 1775 0 2051 0 2230 0 2369 0 2573 0 2801 0 2822 0 2852 0 2858 
COMMENT             0 3035 0 3127 0 3136 0 3215 0 3491 0 4059 0 4176 0 4490 0 4719 0 
COMMENT             4976 0 5154 0 5173 0 5852 0 5915 0 6015 0 6536 0 6793 0 6839 0 6929 
COMMENT             0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AlwI" "ggatc" 10 11 15 643 1 656 0 785 1 984 
COMMENT             0 2703 0 2931 0 3795 0 5566 0 5570 1 5887 0 6350 1 6351 0 6447 1 
COMMENT             6449 0 6535 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 3 2195 0 2852 0 6684 0 
COMMENT             0 "") 
COMMENT        #28=(CRSite (CStringList) "ApaBI" "gcannnnntgc" 9 0 1 2604 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "ApaI" "gggccc" 6 0 4 177 0 2148 0 2286 0 2378 
COMMENT             0 0 "") 
COMMENT        #30=(CRSite (CStringList) "ApoI" "raatty" 2 0 6 1494 0 2210 0 3201 0 
COMMENT             3244 0 4989 0 5000 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "AseI" "attaat" 3 0 1 6030 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 11 204 0 443 0 506 0 947 0 
COMMENT             1466 0 1974 0 2954 0 3713 0 4346 0 5841 0 6063 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 2460 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 12 227 0 563 0 642 0 1011 
COMMENT             0 1061 0 1995 0 2070 0 2475 0 2589 0 4502 0 4765 0 6252 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BanII" "grgcyc" 6 0 7 177 0 729 0 2148 0 2286 
COMMENT             0 2378 0 3578 0 4735 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 5 567 0 2074 0 2479 0 2593 
COMMENT             0 4506 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 3 1916 0 3748 1 4030 0 
COMMENT             0 "") 
COMMENT        #41=(CRSite (CStringList) "BbsI" "gaagac" 9 13 3 1911 0 3753 1 4025 0 0 
COMMENT             "") 
COMMENT        #42=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 4 410 0 2370 1 2466 1 
COMMENT             4128 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BbvI" "gcagc" 14 18 31 21 0 545 1 567 1 612 0 
COMMENT             833 0 859 1 862 1 1171 1 1277 0 1591 1 1779 0 2797 1 2831 0 2887 0 
COMMENT             3058 0 3293 1 3549 1 3625 1 3721 1 3814 1 4477 1 4499 0 4636 0 5160 
COMMENT             1 5771 1 6162 0 6465 1 6671 1 6674 1 6764 0 7096 1 0 "") 
COMMENT        #44=(CRSite (CStringList) "BccI" "ccatc" 10 11 22 133 0 558 0 861 0 1074 
COMMENT             0 1326 0 1527 1 1670 1 1740 1 1848 1 1854 1 2073 0 2409 0 2421 0 
COMMENT             2679 0 2994 0 2999 1 4368 1 4791 1 4823 0 5748 1 6035 1 6159 1 0 "") 
COMMENT        #45=(CRSite (CStringList) "BceAI" "acggc" 18 20 16 785 0 827 0 878 0 
COMMENT             1100 0 1112 0 1160 0 1241 0 1352 0 1442 0 1658 0 2045 0 2607 1 2774 
COMMENT             0 3547 0 4779 0 6593 1 0 "") 
COMMENT        #46=(CRSite (CStringList) "BcefI" "acggc" 18 19 16 785 0 827 0 878 0 
COMMENT             1100 0 1112 0 1160 0 1241 0 1352 0 1442 0 1658 0 2045 0 2608 1 2774 
COMMENT             0 3547 0 4779 0 6594 1 0 "") 
COMMENT        #47=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 3 355 0 4139 0 
COMMENT             5667 1 0 "") 
COMMENT        #48=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 5 2036 1 2576 1 3294 1 
COMMENT             5368 0 6895 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 1615 0 1834 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BfaI" "ctag" 2 0 12 131 0 161 0 1909 0 2149 0 
COMMENT             2461 0 2680 0 3955 0 4369 0 4653 0 6012 0 6347 0 6600 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 1 4161 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 5 43 0 2574 0 2683 0 
COMMENT             4462 0 6087 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BglII" "agatct" 2 0 1 3837 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 4 723 0 2226 0 2871 0 
COMMENT             4172 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "Bme1580I" "gkgcmc" 6 0 15 177 0 540 0 1066 0 
COMMENT             1195 0 1444 0 2022 0 2148 0 2286 0 2378 0 3438 0 3591 0 3909 0 5040 
COMMENT             0 5537 0 6783 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 2 675 0 1958 1 0 "") 
COMMENT        #57=(CRSite (CStringList) "BmrI" "actggg" 12 11 3 2268 0 3863 1 6165 0 0 
COMMENT             "") #58=(CRSite (CStringList) "BmtI" "gctagc" 6 0 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "BplI" "gagnnnnnctc" 25 0 3 1526 0 3040 0 3846 
COMMENT             0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BpmI" "ctggag" 23 21 9 975 0 1470 0 2835 0 
COMMENT             2874 0 3010 1 3057 0 3885 1 4081 0 6136 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 5 410 0 894 0 2370 1 
COMMENT             2466 1 4128 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 5601 0 6469 0 6710 1 
COMMENT             7008 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 5 1784 0 2015 0 2609 0 
COMMENT             4341 0 4806 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 2 2538 0 2964 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 12 57 0 431 0 564 0 2071 
COMMENT             0 2476 0 2590 0 3091 0 3675 0 4443 0 4503 0 5283 0 5665 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 3 1579 0 4234 0 6139 0 0 
COMMENT             "") 
COMMENT        #67=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 34 41 0 52 0 362 0 472 0 
COMMENT             658 0 1054 0 1194 0 1357 0 1381 0 1436 0 1549 0 1796 0 1970 0 1978 
COMMENT             0 2059 0 2117 0 2460 0 2578 0 2659 0 2767 0 2950 0 2998 0 3061 0 
COMMENT             3142 0 3529 0 3601 0 3633 0 3725 0 3885 0 4104 0 4262 0 4446 0 4457 
COMMENT             0 6933 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 6 645 0 1014 0 1917 0 
COMMENT             5909 0 6740 0 6887 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 3 597 1 4318 0 
COMMENT             4845 1 0 "") 
COMMENT        #70=(CRSite (CStringList) "BscAI" "gcatc" 10 12 20 300 0 318 1 780 0 
COMMENT             1127 1 1401 0 1416 0 1561 1 1622 1 2547 0 3820 0 3858 0 3947 0 4502 
COMMENT             1 4538 0 5054 1 5144 0 5507 1 5752 0 5947 1 6999 1 0 "") 
COMMENT        #71=(CRSite (CStringList) "BseMII" "ctcag" 16 14 24 23 1 98 0 424 0 588 
COMMENT             0 599 0 614 1 714 1 885 1 1651 1 2217 1 2361 1 2457 1 2862 1 3789 0 
COMMENT             4142 0 4186 0 4259 0 4406 1 4481 0 5057 0 5718 0 6235 1 6401 1 6810 
COMMENT             1 0 "") 
COMMENT        #72=(CRSite (CStringList) "BseRI" "gaggag" 17 15 7 621 0 1056 0 2199 0 
COMMENT             2811 0 3045 0 3350 1 3411 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BseYI" "cccagc" 2 6 8 499 0 1556 1 2569 0 
COMMENT             2822 1 2980 0 3054 0 4279 1 6789 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 8 840 0 921 0 1154 1 
COMMENT             1251 0 1786 0 2589 1 2994 0 4086 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 7 147 0 243 0 2911 0 4357 
COMMENT             0 5687 0 5836 0 6759 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 7 729 0 2599 0 3438 0 
COMMENT             5040 0 5537 0 5622 0 6783 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 35 59 0 131 0 213 0 
COMMENT             386 0 389 0 464 0 973 0 1195 0 1358 0 2345 0 2485 0 2644 0 2665 0 
COMMENT             2666 0 2773 0 2852 0 2920 0 3415 0 3466 0 3565 0 3634 0 3726 0 3737 
COMMENT             0 3886 0 4104 0 4374 0 4446 0 4587 0 4913 0 4914 0 5167 0 6615 0 
COMMENT             6894 0 7060 0 7078 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 10 1579 0 1948 1 2891 1 
COMMENT             3391 0 4192 1 4234 0 5168 0 5210 1 5363 1 6139 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 4 1948 1 4192 1 5168 0 
COMMENT             5210 1 0 "") 
COMMENT        #81=(CRSite (CStringList) "BsmFI" "gggac" 16 20 10 429 1 1550 0 1574 0 
COMMENT             1713 0 1960 1 2839 0 2963 1 3515 0 3675 1 3686 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 20 177 0 540 0 729 0 
COMMENT             1066 0 1195 0 1444 0 2022 0 2148 0 2286 0 2378 0 2599 0 3438 0 3578 
COMMENT             0 3591 0 3909 0 4735 0 5040 0 5537 0 5622 0 6783 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "Bsp24I" "gacnnnnnntgg" 25 20 6 1813 0 2723 0 
COMMENT             3470 1 5305 0 6399 1 6577 1 0 "") 
COMMENT        #85=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 24 24 1 97 0 423 0 587 
COMMENT             0 598 0 615 1 715 1 886 1 1652 1 2218 1 2362 1 2458 1 2863 1 3788 0 
COMMENT             4141 0 4185 0 4258 0 4407 1 4480 0 5056 0 5717 0 6236 1 6402 1 6811 
COMMENT             1 0 "") 
COMMENT        #86=(CRSite (CStringList) "BspEI" "tccgga" 2 0 1 1917 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 5260 0 5365 0 6373 0 0 
COMMENT             "") 
COMMENT        #88=(CRSite (CStringList) "BspMI" "acctgc" 11 15 2 1468 0 2343 0 0 "") 
COMMENT        #89=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 4353 1 4662 0 5363 0 0 
COMMENT             "") 
COMMENT        #90=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 5 1757 0 2885 0 3472 1 
COMMENT             5971 0 6145 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 9 440 0 494 0 566 0 1176 
COMMENT             0 2172 0 2560 0 3737 0 4701 0 6120 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 1000 0 1886 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "BsrI" "actgg" 7 5 17 261 0 2082 0 2263 0 2712 
COMMENT             0 3266 0 3467 0 3869 1 3902 1 4895 0 5560 0 5730 1 5999 0 6042 0 
COMMENT             6160 0 6566 0 6678 1 6691 1 0 "") 
COMMENT        #94=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 7 15 0 100 0 393 0 395 0 
COMMENT             423 0 4398 0 4483 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "BssSI" "cacgag" 2 6 4 1206 1 5229 1 5536 0 
COMMENT             6920 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 1 2603 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 915 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 1 4333 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 18 216 0 292 1 330 0 1066 
COMMENT             1 1303 1 1432 1 1538 0 1751 0 1865 0 2413 1 2843 0 2944 1 2986 1 
COMMENT             3850 1 5136 1 5759 0 6046 0 6227 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "BstKTI" "gatc" 4 0 28 651 0 793 0 941 0 979 
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FEATURES             Location/Qualifiers
     LTR             1..141
                     /vntifkey="19"
                     /label=L-ITR
     misc_feature    3214..3822
                     /vntifkey="21"
                     /label=WPRE
     polyA_signal    3842..4320
                     /vntifkey="25"
                     /label=hGH\polyA
     LTR             4360..4500
                     /vntifkey="19"
                     /label=R-ITR
     rep_origin      4592..4898
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    5417..6274
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      6425..7092
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    1913..1921
                     /vntifkey="21"
                     /label=linker
     misc_feature    1976..1976
                     /vntifkey="21"
                     /label=cleavage
     misc_feature    1922..1978
                     /vntifkey="21"
                     /label=p2A
     misc_feature    2915..2974
                     /vntifkey="21"
                     /label=TS
     misc_feature    659..1906
                     /vntifkey="21"
                     /label=GCaMP\6m\trunc
     misc_feature    3170..3199
                     /vntifkey="21"
                     /label=HA
     misc_feature    1979..2905
                     /vntifkey="21"
                     /label=ChRmine
     misc_feature    2975..3169
                     /vntifkey="21"
                     /label=Kv2.1
     misc_feature    156..640
                     /vntifkey="21"
                     /label=hSyn
BASE COUNT     1550 a      1993 c      1951 g      1603 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc 
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg 
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta 
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca 
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa 
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc 
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac 
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc 
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat ccgccaccat 
      661 ggtggactca tcacgtcgta agtggaataa gacaggtcac gcagtcagag ctataggtcg 
      721 gctgagctca ctcgagaacg tctatatcaa ggccgacaag cagaagaacg gcatcaaggc 
      781 gaacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgcct accactacca 
      841 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcgt 
      901 gcagtccaaa ctttcgaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 
      961 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg 
     1021 gagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 
     1081 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac 
     1141 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 
     1201 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat 
     1261 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacatccagg agcgcaccat 
     1321 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 
     1381 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 
     1441 gcacaagctg gagtacaacc tgccggacca actgactgaa gagcagatcg cagaatttaa 
     1501 agaggctttc tccctatttg acaaggacgg ggatgggaca ataacaacca aggagctggg 
     1561 gacggtgatg cggtctctgg ggcagaaccc cacagaagca gagctgcagg acatgatcaa 
     1621 tgaagtagat gccgacggtg acggcacaat cgacttccct gagttcctga caatgatggc 
     1681 aagaaaaggg agctacaggg acacggaaga agaaattaga gaagcgttcg gtgtgtttga 
     1741 taaggatggc aatggctaca tcagtgcagc agagcttcgc cacgtgatga caaaccttgg 
     1801 agagaagtta acagatgaag aggttgatga aatgatcagg gaagcagaca tcgatgggga 
     1861 tggtcaggta aactacgaag agtttgtaca aatgatgaca gcgaagacta gtggctccgg 
     1921 agccacgaac ttctctctgt taaagcaagc aggagacgtg gaagaaaacc ccggtcccat 
     1981 ggcacacgca ccaggcaccg accagatgtt ctacgtgggc acaatggacg gctggtatct 
     2041 ggataccaag ctgaactccg tggccatcgg cgcccactgg tcttgcttta tcgtgctgac 
     2101 aatcaccaca ttctacctgg gctatgagtc ctggacatct cggggcccta gcaagagaac 
     2161 ctccttttac gccggctatc aggaggagca gaacctggcc ctgtttgtga atttctttgc 
     2221 catgctgagc tacttcggca agatcgtggc cgacacactg ggccacaact tcggcgatgt 
     2281 gggccccttc atcatcggct tcggcaatta caggtatgcc gactacatgc tgacctgccc 
     2341 aatgctggtg tacgatctgc tgtatcagct gagggccccc tatcgcgtgt cttgtagcgc 
     2401 catcatcttt gccatcctga tgtctggcgt gctggccgag ttctacgcag agggcgaccc 
     2461 taggctgagg aatggcgcct acgcctggta tggctttggc tgtttctggt ttatcttcgc 
     2521 ctactctatc gtgatgagca tcgtggccaa gcagtatagc cggctggccc agctggccca 
     2581 ggatacaggc gccgagcaca gcctgcacgt gctgaagttc gccgtgttta ccttctccat 
     2641 gctgtggatt ctgtttcccc tggtgtgggc catctgccct agaggcttcg gctggatcga 
     2701 cgataactgg acagaggtgg cccactgcgt gtgcgacatc gtggccaagt cctgttacgg 
     2761 ctttgccctg gcccggttca gaaagaccta tgatgaggag ctgtttcggc tgctggagca 
     2821 gctgggacac gacgaggatg agttccagaa gctggagctg gatatgaggc tgagcagcaa 
     2881 tggcgagcgc ctgcggagac tgtctgcggc cgccaagagc aggatcacca gcgagggcga 
     2941 gtacatcccc ctggaccaga tcgacatcaa cgtgcagtcc cagcccatcc tcaacaccaa 
     3001 ggagatggcc ccgcagagca agcctccaga ggagctggag atgagcagca tgcccagccc 
     3061 cgtggcccct ctgcccgcac gcacggaggg cgtcatcgac atgcggagca tgtccagcat 
     3121 tgacagcttc atcagctgtg ccacggactt ccctgaagcc accagattct acccatacga 
     3181 tgttccagat tacgcttaag aattcgatat caagcttatc gataatcaac ctctggatta 
     3241 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 
     3301 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 
     3361 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 
     3421 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 
     3481 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 
     3541 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 
     3601 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg 
     3661 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 
     3721 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 
     3781 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc 
     3841 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact 
     3901 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg 
     3961 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag 
     4021 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct 
     4081 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag 
     4141 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga 
     4201 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca 
     4261 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt 
     4321 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt 
     4381 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 
     4441 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg 
     4501 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc 
     4561 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     4621 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
     4681 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
     4741 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg 
     4801 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     4861 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta 
     4921 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 
     4981 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac 
     5041 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 
     5101 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 
     5161 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 
     5221 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 
     5281 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     5341 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     5401 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     5461 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     5521 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     5581 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     5641 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     5701 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     5761 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     5821 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     5881 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     5941 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     6001 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     6061 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     6121 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     6181 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     6241 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     6301 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     6361 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     6421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     6481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     6541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 
     6601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     6661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     6721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     6781 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     6841 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     6901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     6961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     7021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     7081 gccttttgct cacatgt 
//
