LOCUS       Exported               11381 bp ds-DNA    circular SYN 15-FEB-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    TetR-mKate Prion Sensor plasmid
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 18, 2014 from SnapGene Viewer 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11381
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..995
                     /organism="Cloning vector HO-poly-KanMX4-HO"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:153593"
     source          998..11381
                     /organism="Cloning vector HO-poly-KanMX4-HO"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:153593"
     misc_feature    1..995
                     /note="vector designed for targeted integration to the HO 
                     locus in yeast; plasmid contains two fragments from the HO 
                     promoter inserted in the pUC21-NotI polylinker, along with 
                     the KanMX selectable marker; HO region is bracketed by two 
                     NotI sites, with polylinker sites between the HO and KanMX4
                     regions"
     misc_feature    53..60
                     /note="NotI"
     gene            73..978
                     /note="HO-L taken from 5' upstream region of HO"
                     /note="HO-L taken from 5' upstream region of HO -1815 to 
                     -2721"
     misc_feature    complement(292..311)
                     /label="Primer 6  on 5' end of HO-L; used for sequence"
                     /note="Primer 6 on 5' end of HO-L"
     misc_feature    997..1002
                     /note="BamHI"
     misc_feature    1003..1502
                     /note="Sup35 promoter"
     protein_bind    1521..1645
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1670..1700
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1754..2413
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    1754..2413
                     /note="CAT/CamR"
     CDS             2758..3063
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3104..3228)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3221..3259
                     /note="Space between attR1 site"
     misc_feature    3260..3304
                     /note="NLS"
     CDS             3266..3286
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     misc_feature    3317..3550
                     /note="VP16 Activation Domain"
     misc_feature    3563..3823
                     /note="ZF"
     misc_feature    3839..3868
                     /note="NES"
     misc_feature    3890..4081
                     /note="6x HA"
     CDS             3890..3979
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /note="3xHA"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             3986..4075
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /note="3xHA"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      4101..4339
                     /note="Sup35 Terminator"
     protein_bind    4498..4709
                     /gene="8x-43-8"
                     /bound_moiety="ZF 43-8"
                     /note="8x43-8 binding"
     promoter        4718..4952
                     /note="pCYC1"
     CDS             4953..4965
                     /codon_start=1
                     /label="ORF1"
                     /note="ORF1"
                     /translation="V*TK"
     misc_feature    4953..4960
                     /note="PmeI site"
     misc_feature    4961..4965
                     /note="RBS"
     misc_feature    4966..5010
                     /note="NLS"
     CDS             4972..4992
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     misc_feature    5011..5628
                     /note="TetR"
     CDS             5014..5628
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /note="TetR"
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             5629..6336
                     /codon_start=1
                     /label="ORF1"
                     /note="yomKate2"
                     /translation="GSGGSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADM
                     ALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHRGS"
     misc_feature    6343..6350
                     /note="AscI"
     terminator      6363..6550
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
     terminator      6363..6542
                     /label="yADH1_terminator"
                     /note="yADH1_terminator"
     misc_feature    complement(6584..6603)
                     /label="PCR primer 12 - just 3' of the ADH3 terminator"
                     /note="PCR primer 12 - just 3' of the ADH3 terminator"
     promoter        6625..6968
                     /note="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     misc_feature    6776..6795
                     /label="kanB"
                     /note="kanB"
     CDS             6969..7541
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /note="NrsR"
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     CDS             6969..6975
                     /codon_start=1
                     /gene="KanR2(variant)"
                     /label="KanR2(variant)"
                     /note="KanR2"
                     /note="ORF frame 3"
                     /translation="MG"
     CDS             6976..7541
                     /codon_start=1
                     /label="NATr"
                     /note="NATr"
                     /translation="PLLTTRLTGTAPVSRGTPRPSRHWMGPSPPTPSSASPPPGTASPC
                     GRCRWTRP*PRCSPTTNRTTNRTTGRTATRTPGRSSRTGTTATWRASWSSRTPAGTAG*
                     PSRTSRSPRSTGGTGSGAR*WGSRRSSPASGAPGTSGWRSPTSTHRRSTRTGGWGSPSA
                     AWTPPCTTAPPRTASRRST*ACPAP"
     terminator      7547..7744
                     /note="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    complement(7783..7792)
                     /label="R2"
                     /note="R2"
     gene            7798..8298
                     /note="HO-R matches 1190 of gene to 1690"
     misc_feature    8184..8203
                     /label="PCR primer 11 for 3' end of HO-R"
                     /note="PCR primer 11 for 3' end of HO-R"
     misc_feature    8306..8313
                     /note="NotI"
     primer_bind     8314..8330
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(8345..8363)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8370..8386)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9258..9362
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             9363..10223
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10394..10982
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11270..11291
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    11346..11362
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11370..5
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgaccatga ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc
       61 cagctgaagc ttaattatcc tgggcacgag tgaaacaaag ctaaaacctt tatttagcat
      121 ggccattgaa tgtaacaatt atatatatcg caagcacaaa aaatcaagga gagagaacta
      181 ccactttgtt catgtgtaca atgttcatta tctccataag caaaaaaaaa aaatagaaaa
      241 catatgctat aaggttgata ttctcacgag taagcggcac ttgctactta ttgacattgc
      301 agatttttgg ctacagaaat agtatattag agattataat tgctaatcaa atcaaaatat
      361 aaaattagta aaccaaacca tttataccct tccttagtag ttatggattg ttttttaatg
      421 atatttctgc aaaccaaaga aagattgtta tccagataga atttagtttt gatattcatt
      481 tttttgttga agattgaacg ccatatctgg gcctcataat tcaaaagacg gtgccattat
      541 cggtagcgtt tcgcattgta ctggatttca gaaatttcac agttgatgaa tcgaaaagaa
      601 tggtctcatt gcaacacgta aggttaagat gtcccttttt accattatag gcaataaatg
      661 aatcataaaa cgaccgtata ctggtgaaat agtagggaga acgagtacct gtagtaaaaa
      721 gtataaatca tagttaatcg ggcaatgtcc ctcgatcaag gagtattgtg tcatgttcga
      781 gacaaacgcc aacatttttg tttcttttgg acaaatgttg tttgcattta tgatccgtta
      841 tattttgatc taatgtagag ttgcacgtag ttcttactgg caaagaaatc gatgcatacc
      901 aaaaaagaat aaaggtgata tttgatcttt accgtttagt tccaacgtaa aattgtgcct
      961 ttggacttaa aatggcgtcg tacgctgcag gtcgacggat cccaaccaca caaaaatcat
     1021 acaacgaatg gtatatgctt catttctttg tttcgcatta gctgcgctat ttgactcaaa
     1081 ttattatttt ttactaagac gacgcgtcac agtgttcgag tctgtgtcat ttcttttgta
     1141 attctcttaa accacttcat aaagttgtga agttcatagc aaaattcttc cgcaaaaaga
     1201 tgaatcttag ttctcagccc accaaaagag gtacatgcta agatcataca gaagttattg
     1261 tcacttctta ccttgctctt aaatgtacat tacaaccggg tattatatct tacatcatcg
     1321 tataatatga tctttcttta tggagaaaat ttttttttca ctcgaccaaa gctcccattg
     1381 cttctgaaga gtgtagtgta tattggtaca tcttctcttg aaagactcca ttgtactgta
     1441 acaaaaagcg gtttcttcat cgacttgctc ggaataacat ctatatctgc ccactagcaa
     1501 caactagtgg atcccccatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
     1561 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     1621 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta
     1681 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc
     1741 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     1801 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     1861 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     1921 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
     1981 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     2041 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     2101 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     2161 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     2221 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     2281 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg
     2341 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     2401 gggcggggcg taaacgccgc gtggatccgg cttactaaaa gccagataac agtatgcgta
     2461 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
     2521 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     2581 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     2641 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     2701 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
     2761 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     2821 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     2881 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     2941 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     3001 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     3061 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     3121 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     3181 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtga tgggctgcag
     3241 gaattcgata tcaagcttaa tggctccaaa gaagaagaga aaggttggta ttcatggtgt
     3301 tccagttgat ggtggtttac aagctccacc aactgatgtt tctttgggtg atgaattgca
     3361 tttggatggt gaagatgttg ctatggctca tgctgatgct ttggatgatt ttgatttgga
     3421 tatgttgggt gacggtgatt ctccaggtcc aggttttact ccacatgatt ctgctccata
     3481 tggtgcttta gatatggctg actttgaatt cgaacaaatg ttcaccgatg ccttgggtat
     3541 tgatgaatat ggtggtggtg ctagaccagg tgaaagacca tttcaatgta gaatctgcat
     3601 gagaaacttc tccagacaag atagattgga tagacatacc agaactcata ctggtgaaaa
     3661 gccattccaa tgcagaatat gtatgagaaa tttttcccaa aaagaacact tggccggtca
     3721 tttgagaaca cacacaggtg aaaaaccttt tcaatgcaga atatgcatga gaaactttag
     3781 tagaagagac aacttgaaca gacacttgaa aacccatttg agaggttctg ccggcgaatt
     3841 ggctttgaaa ttggctggtt tggatatcaa ttctggttct gctgctagat acccatatga
     3901 tgttccagat tatgctggtt acccttacga tgttcctgac tacgcaggtt cttatccata
     3961 cgatgtacct gattatgcct caagatatcc ttatgacgtc cctgattacg ccggttatcc
     4021 atatgacgta ccagactacg ctggtagtta cccatacgac gtcccagatt acgcttcttc
     4081 ataatgactc gagtcatgta atttcttgca aacataagta aatgcaaaca caataatacc
     4141 gatcataaag cattttcttc tatattaaaa aacaaggttt aataaagctg ttatatatat
     4201 atatatatat atagacgtat aattagttta gttctttttg taccatatac cataaacaag
     4261 gtaaacttca cctctcaata tatctagaat ttcataaaaa tatctagcaa ggtttcaact
     4321 ccttcaatca cgttttcatc actcaaccct atctcggtct attcttttga tttataaggg
     4381 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4441 aattttaaca aaatattaac gtttacaatt tcctgatgcg gtattttctt taattaaaga
     4501 gtgaggactc gaaaatatta atagagtgag gactcgaaaa tattaataga gtgaggactc
     4561 gaaaatatta atagagtgag gactcgaaaa tattaatatc gatagagtga ggactcgaaa
     4621 atattaatag agtgaggact cgaaaatatt aatgaattca gagtgaggac tcgaaaatat
     4681 taatagagtg aggactcgaa aatattaatg gccggcccag atccgccagg cgtgtatata
     4741 tagcgtggat ggccaggcaa ctttagtgct gacacataca ggcatatata tatgtgtgcg
     4801 acgacacatg atcatatggc atgcatgtgc tctgtatgta tataaaactc ttgttttctt
     4861 cttttctcta aatattcttt ccttatacat taggaccttt gcagcataaa ttactatact
     4921 tctatagaca cacaaacaca aatacacaca ctgtttaaac aaaaaatggc tccaaagaag
     4981 aagagaaagg ttggtattca tggtgttcca tcaagattag ataaaagtaa agtgattaac
     5041 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc
     5101 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg
     5161 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa
     5221 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta
     5281 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa
     5341 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta
     5401 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat
     5461 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga
     5521 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa
     5581 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtctgg ttctggtggt
     5641 tctgaattga tcaaagaaaa catgcacatg aagttgtaca tggaaggtac tgttaacaac
     5701 catcatttca agtgtacctc tgaaggtgaa ggtaaaccat atgaaggtac tcaaaccatg
     5761 agaatcaaag ctgttgaagg tggtccattg ccatttgctt ttgatatttt ggctacctcc
     5821 ttcatgtacg gttctaagac tttcattaac cacacccaag gtatcccaga tttcttcaaa
     5881 caatctttcc cagaaggttt cacttgggaa agagttacta cttacgaaga tggtggtgtt
     5941 ttgactgcta ctcaagatac ttctttacaa gacggttgct tgatctacaa cgttaagatt
     6001 agaggtgtca acttcccatc taatggtcca gttatgcaaa aaaagacttt gggttgggaa
     6061 gcttctactg aaacattata tccagctgat ggtggtttgg aaggtagagc tgatatggct
     6121 ttgaaattgg ttggtggtgg tcatttgatc tgcaacttga aaactaccta cagatctaag
     6181 aagccagcca agaatttgaa aatgccaggt gtttactacg tcgatagaag attggaaaga
     6241 atcaaagaag ccgacaaaga aacctacgtt gaacaacacg aagttgctgt tgctagatat
     6301 tgtgatttgc catccaaatt aggtcataga ggatcttaat agggcgcgcc acttctaaat
     6361 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     6421 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     6481 ctttcctgta ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca
     6541 cctctaccgg cagatccgct agggataaca gggtaatata gatctgttta gcttgcctcg
     6601 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
     6661 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
     6721 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
     6781 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
     6841 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
     6901 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
     6961 aaacaaccat gggtaccact cttgacgaca cggcttaccg gtaccgcacc agtgtcccgg
     7021 gggacgccga ggccatcgag gcactggatg ggtccttcac caccgacacc gtcttccgcg
     7081 tcaccgccac cggggacggc ttcaccctgc gggaggtgcc ggtggacccg cccctgacca
     7141 aggtgttccc cgacgacgaa tcggacgacg aatcggacga cggggaggac ggcgacccgg
     7201 actcccggac gttcgtcgcg tacggggacg acggcgacct ggcgggcttc gtggtcgtct
     7261 cgtactccgg ctggaaccgc cggctgaccg tcgaggacat cgaggtcgcc ccggagcacc
     7321 gggggcacgg ggtcgggcgc gcgttgatgg ggctcgcgac ggagttcgcc cgcgagcggg
     7381 gcgccgggca cctctggctg gaggtcacca acgtcaacgc accggcgatc cacgcgtacc
     7441 ggcggatggg gttcaccctc tgcggcctgg acaccgccct gtacgacggc accgcctcgg
     7501 acggcgagca ggcgctctac atgagcatgc cctgccccta atcagtactg acaataaaaa
     7561 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
     7621 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
     7681 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
     7741 actgctgtcg attcgatact aacgccgcca tccagtgtcg aaaacgagct cgaattcctg
     7801 ggggaacaac ttcacagaat gttttgtcat attgtcgaag tggtcacaaa acaagagaag
     7861 ttccgccaat tataaaaagg gaacccgtat atttcagctt cacggatgat ttccagggtg
     7921 agagtactgt atatgggctt acgatagaag gccataaaaa tttcttgctt ggcaacaaaa
     7981 tagaagtgaa atcatgtcga ggctgctgtg tgggagaaca gcttaaaata tcacaaaaaa
     8041 agaatctaaa acactgtgtt gcttgtccca gaaagggaat caagtatttt tataaagatt
     8101 ggagtggtaa aaatcgagta tgtgctagat gctatggaag atacaaattc agcggtcatc
     8161 actgtataaa ttgcaagtat gtaccagaag cacgtgaagt gaaaaaggca aaagacaaag
     8221 gcgaaaaatt gggcattacg cccgaaggtt tgccagttaa aggaccagag tgtataaaat
     8281 gtggcggaat cttacagtgg cctatgcggc cgctctagaa ctagtggatc gatccccaat
     8341 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     8401 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8461 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8521 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     8581 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     8641 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     8701 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggtgggc
     8761 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8821 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     8881 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8941 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     9001 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     9061 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     9121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     9181 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     9241 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9301 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9361 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9421 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9481 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9541 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9601 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9661 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9721 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9781 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9841 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9901 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9961 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10021 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10081 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10141 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10201 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10261 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10321 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10381 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10441 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10501 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
    10561 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10621 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10681 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10741 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
    10801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10921 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10981 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11041 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11101 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    11161 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    11221 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    11281 agctcactca ttaggcaccc caggctttac actttatgct tccgcggctc gtatgttgtg
    11341 tggaattgtg agcggataac aatttcacac aggaaacagc t
//
