LOCUS       pSI-535                 4349 bp ds-DNA     circular     25-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        2069..2099
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     origin          3906..4212
                     /label="f1_origin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     feature         3728..3888
                     /label="lacZ_a"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            126..986
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3094..3171
                     /label="SV40 polyA signal"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2964..2983
                     /label="Guide"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     rep_origin      1081..1763
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        56..84
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(3705..3732)
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2984..3065
                     /label="scaffold"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        2168..2186
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        2723..2955
                     /label="hU6_promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_binding    2103..2125
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3066..3066
                     /label="EcoR1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
       61 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
      121 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
      181 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG
      241 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
      301 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT
      361 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG
      421 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG
      481 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA
      541 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC
      601 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA
      661 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC
      721 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC
      781 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG
      841 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA
      901 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG
      961 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA
     1021 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC
     1081 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
     1141 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
     1201 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
     1261 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT
     1321 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
     1381 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
     1441 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
     1501 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
     1561 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
     1621 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
     1681 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
     1741 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
     1801 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
     1861 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
     1921 CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA
     1981 GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA
     2041 GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT
     2101 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA
     2161 AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA GCTGATTTAA ATGGGGACAG
     2221 CCCCCCCCCA AAGCCCCCAG GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG
     2281 AGCCGCCCGG GGCTCCGCTC CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG
     2341 CGGGGACAGC CCGGGCACGG GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG
     2401 CTGCTCTTTG AGCCTGCAGA CACCTGGGGG GATACGGGGA AAAAGCTTTA GGCTGAGTGC
     2461 ACGGGGACAG CCCCCCCCCA AAGCCCCCAG GGATGTAATT ACGTCCCTCC CCCGCTAGGG
     2521 GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC
     2581 CGGCAGCGTG CGGGGACAGC CCGGGCACGG GGAAGGTGGC ACGGGATCGC TTTCCTCTGA
     2641 ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA CACCTGGGGG GATACGGGGA AAAAGCTTTA
     2701 GGCTGAAGAT CTAGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA TACGATACAA
     2761 GGCTGTTAGA GAGATAATTA GAATTAATTT GACTGTAAAC ACAAAGATAT TAGTACAAAA
     2821 TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT TATGTTTTAA
     2881 AATGGACTAT CAtatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     2941 cttgtggaaa ggacgaaaca ccGCACGGTC ACCCTGACAC GCTgttttag agctagaaat
     3001 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     3061 tttttgAATT CCTGACGCAC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT
     3121 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCCTCGA
     3181 ATCACTAGTG GGGACAGCCC CCCCCCAAAG CCCCCAGGGA TGTAATTACG TCCCTCCCCC
     3241 GCTAGGGGGC AGCAGCGAGC CGCCCGGGGC TCCGCTCCGG TCCGGCGCTC CCCCCGCATC
     3301 CCCGAGCCGG CAGCGTGCGG GGACAGCCCG GGCACGGGGA AGGTGGCACG GGATCGCTTT
     3361 CCTCTGAACG CTTCTCGCTG CTCTTTGAGC CTGCAGACAC CTGGGGGGAT ACGGGGAAAA
     3421 AGCTTTAGGC TGAGTGCACG GGGACAGCCC CCCCCCAAAG CCCCCAGGGA TGTAATTACG
     3481 TCCCTCCCCC GCTAGGGGGC AGCAGCGAGC CGCCCGGGGC TCCGCTCCGG TCCGGCGCTC
     3541 CCCCCGCATC CCCGAGCCGG CAGCGTGCGG GGACAGCCCG GGCACGGGGA AGGTGGCACG
     3601 GGATCGCTTT CCTCTGAACG CTTCTCGCTG CTCTTTGAGC CTGCAGACAC CTGGGGGGAT
     3661 ACGGGGAAAA AGCTTTAGGC TGAGGCGCGC CTGTACCCAA TTCGCCCTAT AGTGAGTCGT
     3721 ATTACAATTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC
     3781 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC
     3841 GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAC GCGCCCTGTA
     3901 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA
     3961 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT
     4021 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC
     4081 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT
     4141 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC
     4201 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC
     4261 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA
     4321 ACAAAATATT AACGCTTACA ATTTAGGTG
//