LOCUS       Exported               11733 bp ds-DNA     circular SYN 21-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..4120
                     /label=miniW+
     misc_feature    4121..4182
                     /label=GAGA
     misc_feature    4219..4565
                     /label=14 UAS GAL4 binding sites
     misc_feature    4577..4807
                     /label=P-transposase promoter
     misc_feature    4807..4808
                     /label=MCS
     misc_feature    4813..6405
                     /label=mNeonGreen-3XFLAG-crk
     misc_feature    6406..6413
                     /label=MCS
                     /label=MCS(1)
     misc_feature    6468..7888
                     /label=K10 3' UTR
     misc_feature    7888..8111
                     /label=attB site
     misc_feature    8143..8375
                     /label=3' P-element sequence
     misc_feature    9284..10144
                     /label=ampR
     misc_feature    10145..11138
                     /label=5' P-element sequence
ORIGIN
        1 ctagttccag tgaaatccaa gcattttcta aattaaatgt attcttatta ttatagttgt
       61 tatttttgat atatataaac aacactatta tgcccaccat ttttttgaga tgcatctaca
      121 caaggaacaa acactggatg tcactttcag ttcaaattgt aacgctaatc actccgaaca
      181 ggtcacaaaa aattacctta aaaagtcata atattaaatt agaataaata tagctgtgag
      241 ggaaatatat acaaatatat tggagcaaat aaattgtaca tacaaatatt tattactaat
      301 ttctattgag acgaaatgaa ccactcggaa ccatttgagc gaaccgaatc gcgcggaact
      361 aacgacagtc gctccaaggt cgtcgaacaa aaggtgaatg tgttgcggag agcgggtggg
      421 agacagcgaa agagcaacta cgaaacgtgg tgtggtggag gtgaattatg aagagggcgc
      481 gcgatttgaa aagtatgtat ataaaaaata tatcccggtg ttttatgtag cgataaacga
      541 gtttttgatg taaggtatgc aggtgtgtaa gtcttttggt tagaagacaa atccaaagtc
      601 tacttgtggg gatgttcgaa ggggaaatac ttgtattcta taggtcatat cttgttttta
      661 ttggcacaaa tataattaca ttagcttttt gagggggcaa taaacagtaa acacgatggt
      721 aataatggta aaaaaaaaaa acaagcagtt atttcggata tatgtcggct actccttgcg
      781 tcgggcccga agtcttagag ccagatatgc gagcacccgg aagctcacga tgagaatggc
      841 cagacccacg tagtccagcg gcagatcggc ggcggagaag ttaagcgtct ccaggatgac
      901 cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag ctaatttcgc ccggctccac
      961 gtccgcccat tggttaatca gcagaccctc gttggcgtaa cggaaccatg agaggtacga
     1021 caaccatttg aggtatactg gcaccgagcc cgagttcaag aagaagccgc caaagagcag
     1081 gaatggtatg ataaccggcg gacccacaga cagcgccatc gaggtcgagg agctggcgca
     1141 ggatattaga tatccgaagg acgttgacac attggccacc agagtgacca gcgccaggca
     1201 gttgaagaag tgcagcactc cggcccgcag tccgatcatc ggataggcaa tcgccgtgaa
     1261 gaccagtggc actgtgagaa aaagcggcaa ttcggcaatc gttttgccca gaaagtatgt
     1321 gtcacagcga taaagtcgac ttcgggcctc cctcataaaa actggcagct ctgaggtgaa
     1381 cacctaaatc gaatcgattc attagaaagt tagtaaatta ttgaaatgca aatgtattct
     1441 aaacatgact tacatttatc gtggcaaaga cgttttgaaa ggtcatgttg gtcaggaaga
     1501 ggaagatggc tccgttgata ttcatcacac ccacttgcgt gagttgttgg cccaaaaaga
     1561 tgaggccaat caagatggca accatctgca aattaaaatg ttactcgcat ctcattaata
     1621 ttcgcgagtt aaatgaaatt tatttatctt ctgcaaaact ataaactata catctcattg
     1681 aaaaaaacta agaagggtgt ggaatcaggc aattctatct aaaatctagc gaatttgttt
     1741 ccaagaattg taagcgttat atcatttgtt tccactggaa ccactcaccg ttgtctgaat
     1801 aagtcgcact tttacgagga gtggttcctt gagcaccgac agccaggatc gccacaggac
     1861 cgcccggaac tgcatgaacc aggtggcctt gtaggtgtac ccattctccg gctgctccag
     1921 tggcttctcc agatttttgg tggccaacaa ctgctccata tcccgggcta ctttgctaat
     1981 ggcaaaattg tcgcatatct tggcgatccg atcacgggac tcgatctccc gtccgggcac
     2041 aacggccaac acctgtacgt aaaagtccgc cggattgtag ttggtaggac actgggcacc
     2101 cacgctggat aggagttgag atgttatgta atactagata cccttaataa acacatcgaa
     2161 ctcactagga aaagaagtcg acggcttcgc tgggagtgcc caagaaagct accctgccct
     2221 cggccatcag aaggatcttg tcaaagagct caaacagctc ggaagacggc tgatgaatgg
     2281 tcaggatgac ggtcttgccc ttctgcgaca gcttcttcag cacctggacg acgctgtggg
     2341 cggtaaagga gtccagtccg gaggtgggct catcgcagat cagaagcggc ggatcggtta
     2401 gagcctcgga ggcgaatgcc agacgcttcc tttctccgcc ggacagacct ttcaccctgc
     2461 cgggcacacc gatgatcgtg tgctgacatt tgctgagcga aagctcctgg atcacctgat
     2521 ccacgcgggc cactcgctgc cgataggtca gatgtcgtgg catccgcacc atggcttgga
     2581 aaatcaggtg ttccctggcc gttagggagc cgataaagag gtcatcctgc tggacatagg
     2641 cgcacctggc ctgcatctcc ttggcgtcca caggttggcc attgagcagt cgcatcccgg
     2701 atggcgatac ttggatgccc tgcggcgatc gaaaggcaag ggcattcagc agggtcgtct
     2761 ttccggcacc ggaactgccc atcacggcca aaagttcgcc cggataggcc acgccgcaaa
     2821 ctgagtttca aattggtaat tggacccttt attaagattt cacacagatc agccgactgc
     2881 gaatagaaac tcaccgttct tgagcaaatg tttcctgggc gccggtatgt gtcgctcgtt
     2941 gcagaatagt ccgcgtgtcc ggttgaccag ctgccgccat ccggagcccg gctgattgac
     3001 cgccccaaag atgtccatat tgtgccaggc ataggtgagg ttctcggcta gttggccgct
     3061 ccctgaaccg gagtcctccg gcggactggg tggccggagc gtgccgtagt ttttggcctg
     3121 cccgaagccc tggttaatgc agctctgcga agccgctccg ctgtcaccct gcaatgatag
     3181 gggatctcaa atatcaacta caagcgttat gctcatctaa ccccgaacaa aaagtacccc
     3241 gaagtatcct acgaagtagg tttatacttt tatttatttt ttgtgcatct aggatcagct
     3301 taaaatatct ggttgttata ttttttgtaa aaaagaatat agtcgaaaat gaatgccttt
     3361 agatgtcttg atcatgatat gatctcaaaa attgtcttat atagcgagaa cagctaccag
     3421 aataatctgt ttcgtgtcac tatttgtttg tgcaattgcg gtttgggatt tttgtgggtc
     3481 gcagttctca cgccgcagac aatttgatgt tgcaatcgca gttcctatag atcaagtgaa
     3541 cttaagatgt atgcacatgt actactcaca ttgttcagat gctcggcaga tgggtgtttg
     3601 ctgcctccgc gaattaatag ctcctgatcc tcttggccca ttgccgggat ttttcacact
     3661 ttcccctgct tacccaccca aaaccaatca ccaccccaat cactcaaaaa acaaacaaaa
     3721 ataagaagcg agaggagttt tggcacagca ctttgtgttt aattgatggc gtaaaccgct
     3781 tggagcttcg tcacgaaacc gctgacaaaa tgcaactgaa ggcggacatt gacgctacgt
     3841 aacgctacaa acggtggcga aagagatagc ggacgcagcg gcgaaagaga cggcgatatt
     3901 tctgtggaca gagaaggagg caaacagcgc tgactttgag tggaatgtca ttttgagtga
     3961 gaggtaatcg aaagaacctg gtacatcaaa tacccttgga tcgaagtaaa tttaaaactg
     4021 atcagataag ttcaatgata tccagtgcag taaaaaaaaa aaatgttttt tttatctact
     4081 ttccgcaaaa atgggtttta ttaacttaca tacatactag aattggccgc tctagccccc
     4141 cctcgaatgt tctctctctt ctcttctctc tctctttctc gaatgttctc tctcttctct
     4201 tctctctctc tttctcgagg tcatcaagct taggcctcca aggcggagta ctgtcctccg
     4261 ggctggcgga gtactgtcct ccggcaaggc ggagtactgt cctccgggct ggcggagtac
     4321 tgtcctccgg caaggcggag tactgtcctc cgggctggcg gagtactgtc ctccggcaag
     4381 gcggagtact gtcctccggg ctggcggagt actgtcctcc ggcaaggcgg agtactgtcc
     4441 tccgggctgg cggagtactg tcctccggca aggcggagta ctgtcctccg ggctggcgga
     4501 gtactgtcct ccggcaaggc ggagtactgt cctccgggct ggcggagtac tgtcctccgg
     4561 caagggtcga gtcgatagcc gaagcttacc gaagtataca cttaaattca gtgcacgttt
     4621 gcttgttgag aggaaaggtt gtgtgcggac gaattttttt ttgaaaaccg gtgatagagc
     4681 ctgaaccaga aaagataaaa gaaggctata ccagtgggag tacacaaaca gagtaagttt
     4741 gaatagtaaa aaaaatcatt tatgtaaaca ataacgtgac tgtgcgttag gtcctgttca
     4801 ttggtaccca ccatggtttc caaaggtgaa gaagacaata tggcttccct gcctgcaacc
     4861 cacgagttgc acatttttgg atcgatcaac ggcgtcgatt ttgatatggt aggtcaggga
     4921 acgggaaacc ctaatgacgg ttatgaagaa ctcaatctga agtcgacaaa gggtgatctc
     4981 caattttcgc cttggattct cgtaccgcac attggatatg gatttcatca atatttgcca
     5041 tatccagacg gaatgtcccc gttccaggcg gctatggtcg atggatccgg ttaccaggtc
     5101 cacaggacaa tgcagttcga ggacggcgca tcgctcacgg ttaattaccg ttacacgtac
     5161 gagggtagtc atataaaggg cgaggcacag gtgaaaggaa ccggattccc cgccgatggc
     5221 ccggttatga ccaattcgct cactgcggct gattggtgtc ggtccaaaaa aacatatcct
     5281 aacgacaaaa ctataatatc caccttcaaa tggtcctaca cgaccggaaa tggaaagcgt
     5341 tatcgttcga cggctcggac cacttatact ttcgctaagc ccatggcggc aaactacctc
     5401 aagaatcagc ctatgtatgt attccgaaaa acagagctca agcacagtaa aacggagctg
     5461 aatttcaagg agtggcaaaa agctttcacg gacgttatgg gcatggatga actctacaag
     5521 atggactaca aggatcatga cggtgactac aaagaccatg atatagatta caaggatgac
     5581 gacgacaaga tggatacatt tgacgtttct gataggaaca gctggtactt tggtcccatg
     5641 tctagacagg atgctactga agttttgatg aacgaacgcg agcggggagt gtttttagtc
     5701 cgtgatagta actcgatagc aggggattat gtactttgtg taagagaaga tacaaaagtt
     5761 agcaactaca tcattaacaa agttcaacaa caggatcaaa tcgtttaccg cattggggat
     5821 cagtcttttg acaatctacc gaaactctta actttttaca ctcttcatta tttggataca
     5881 acccctttaa aacggcctgc gtgtagaagg gtggaaaaag taataggaaa gttcgatttc
     5941 gttggcagcg atcaagatga tttacctttt caaagaggtg aagttttaac aatagttcga
     6001 aaagacgagg atcaatggtg gactgcgcgt aactcctcgg ggaaaattgg tcaaataccg
     6061 gttccctata tacaacagta tgacgattat atggatgaag atgctattga taaaaacgaa
     6121 ccttccattt cgggatctag caatgtattt gaaagtactc ttaaaaggac agatttaaat
     6181 cgaaaactac ctgcatacgc ccgcgtaaaa cagtcaaggg tccctaacgc atacgataag
     6241 actgcattaa aattggaaat aggtgacatt attaaagtca ctaaaacaaa cattaatggg
     6301 caatgggagg gagaattaaa tggaaaaaat ggtcattttc ccttcacgca cgttgaattt
     6361 gtcgatgatt gtgatttaag caaaaactcc acagaaatat gctaagcatg catctagagt
     6421 cgacctcgaa cgttaacgtt aacgtaacgt taactcgagg agcttgataa cattatacct
     6481 aaacccatgg tcaagagtaa acatttctgc ctttgaagtt gagaacacaa ttaagcatcc
     6541 cctggttaaa cctgacattc atacttgtta atagcgccat aaacatagca ccaattcgaa
     6601 gaatacttgt taatagcgcc ataaacatag caccaatttc gaagaaactc agttaaaagc
     6661 aattagcaat tagcaattag caataactct gctgacttca aaacgagaag agttgcaagt
     6721 atttgtaagg cacagtttat agaccaccga cggctcatta gggctcgtca tgtaactaag
     6781 cgcggtgaaa cccaattgaa catatagtgg aattattatt atcaatgggg aagatttaac
     6841 cctcaggtag caaagtaatt taattgcaaa tagagagtcc taagactaaa taatatattt
     6901 aaaaatctgg ccctttgacc ttgcttgtca ggtgcatttg ggttcaatcg taagttgctt
     6961 ctatataaac actttcccca tccccgcaat aatgaagaat accgcagaat aaagagagat
     7021 ttgcaacaaa aaataaaggc attgcgaaaa ctttttatgg gggatcatta cactcgggcc
     7081 tacggttaca attcccagcc acttaagcga caagtttggc caacaatcca tctaatagct
     7141 aatagcgcaa tcactggtaa tcgcaagagt atataggcaa tagaacccat ggatttgacc
     7201 aaaggtaacc gagacaatgg agaagcaaga ggatttcaaa ctgaacaccc acagtgctgt
     7261 gtactaccac tggcgcgttt gggagctcac tggcctgatg cgtcctccgg gcgtttcaag
     7321 cctgctttac gtggtatact ccattacggt caacttggtg gtcaccgtgc tgtttccctt
     7381 gagcttgctg gccaggctgc tgttcaccac caacatggcc ggattgtgcg agaacctgac
     7441 cataactatt accgatattg tggccaattt gaagtttgcg aatgtgtaca tggtgaggaa
     7501 gcagctccat gagattcgct ctctcctaag gctcatggac gctagagccc ggctggtggg
     7561 cgatcccgag gagatttctg ccttgaggaa ggaagtgaat atcgcacagg gcactttccg
     7621 cacctttgcc agtattttcg tatttggcac tactttgagt tgcgtccgcg tggtcgttcg
     7681 cccggatcga gagctcctgt atccggcctg gttcggcgtt gactggatgc actccaccag
     7741 aaactatgtg ctcatcaata tctaccagct cttcggcttg atagtgcagg ctatacagaa
     7801 ctgcgctagt gactcctatc cgcctgcgtt tctctgcctg ctcacgggtc atatgcgtgc
     7861 tttggagctg agggtgcggc ggattggcgt cgacgatgta ggtcacggtc tcgaagccgc
     7921 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct
     7981 ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac gcgcggcgca
     8041 ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg
     8101 cgacggcgtc gacgatgtag gtcacggcgg gcagccaagc tttgcgtact cgcaaattat
     8161 taaaaataaa actttaaaaa taatttcgtc taattaatat tatgagttaa ttcaaacccc
     8221 acggacatgc taagggttaa tcaacaatca tatcgctgtc tcactcagac tcaatacgac
     8281 actcagaata ctattccttt cactcgcact tattgcaagc atacgttaag tggatgtctc
     8341 ttgccgacgg gaccacctta tgttatttca tcatggtctg gccattctca tcgtgagctt
     8401 ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt agccgacata
     8461 tatccgaaat aactgcttgt tttttttttt taccattatt accatcgtgt ttactgttta
     8521 ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta
     8581 tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta
     8641 accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa
     8701 caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac
     8761 ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca
     8821 cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcgggtg
     8881 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8941 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     9001 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     9061 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     9121 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     9181 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9241 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9301 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9361 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9421 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9481 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     9541 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9601 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9661 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9721 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9781 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9841 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9901 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9961 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10021 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10081 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10141 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10201 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10441 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    10501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    10621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    10681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    10741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    10801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    10861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcct tcttcttgaa
    10921 ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc
    10981 caacgagggt ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac
    11041 atcgtcgaac accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc
    11101 cgccgccgat ctgccgctgg actacgtggg tctggcccat gatgaaataa cataaggtgg
    11161 tcccgtcgat agccgaagct taccgaagta tacacttaaa ttcagtgcac gtttgcttgt
    11221 tgagaggaaa ggttgtgtgc ggacgaattt ttttttgaaa acattaaccc ttacgtggaa
    11281 taaaaaaaaa tgaaatattg caaattttgc tgcaaagctg tgactggagt aaaattaatt
    11341 cacgtgccga agtgtgctat taagagaaaa ttgtgggagc agagccttgg gtgcagcctt
    11401 ggtgaaaact cccaaatttg tgatacccac tttaatgatt cgcagtggaa ggctgcacct
    11461 gcaaaaggtc agacatttaa aaggaggcga ctcaacgcag atgccgtacc tagtaaagtg
    11521 atagagcctg aaccagaaaa gataaaagaa ggctatacca gtgggagtac acaaacagag
    11581 taagtttgaa tagtaaaaaa aatcatttat gtaaacaata acgtgactgt gcgttaggtc
    11641 ctgttcattg tttaatgaaa ataagagctt gagggaaaaa attcgtactt tggagtacga
    11701 aatgcgtcgt ttagagcagc agccgaatta att
//
