LOCUS       Exported               12462 bp ds-DNA     circular SYN 04-JUL-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12462
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..62
                     /label=GAGA
     misc_feature    99..445
                     /label=14 UAS GAL4 binding sites
     misc_feature    457..687
                     /label=P-transposase promoter
     misc_feature    687..688
                     /label=MCS
     CDS             693..2120
                     /label=tdTomato
     misc_feature    2121..2128
                     /label=MCS
                     /label=MCS(1)
     misc_feature    2129..3014
                     /label=crk
     misc_feature    3015..3022
                     /label=MCS
                     /label=MCS(2)
     misc_feature    3077..4497
                     /label=K10 3' UTR
     misc_feature    4497..4720
                     /label=attB site
     misc_feature    4752..4984
                     /label=3' P-element sequence
     misc_feature    5893..6753
                     /label=ampR
     misc_feature    6754..7747
                     /label=5' P-element sequence
     misc_feature    8343..12462
                     /label=miniW+
ORIGIN
        1 aattggccgc tctagccccc cctcgaatgt tctctctctt ctcttctctc tctctttctc
       61 gaatgttctc tctcttctct tctctctctc tttctcgagg tcatcaagct taggcctcca
      121 aggcggagta ctgtcctccg ggctggcgga gtactgtcct ccggcaaggc ggagtactgt
      181 cctccgggct ggcggagtac tgtcctccgg caaggcggag tactgtcctc cgggctggcg
      241 gagtactgtc ctccggcaag gcggagtact gtcctccggg ctggcggagt actgtcctcc
      301 ggcaaggcgg agtactgtcc tccgggctgg cggagtactg tcctccggca aggcggagta
      361 ctgtcctccg ggctggcgga gtactgtcct ccggcaaggc ggagtactgt cctccgggct
      421 ggcggagtac tgtcctccgg caagggtcga gtcgatagcc gaagcttacc gaagtataca
      481 cttaaattca gtgcacgttt gcttgttgag aggaaaggtt gtgtgcggac gaattttttt
      541 ttgaaaaccg gtgatagagc ctgaaccaga aaagataaaa gaaggctata ccagtgggag
      601 tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca ataacgtgac
      661 tgtgcgttag gtcctgttca ttggtaccca ccatggtgag caagggcgag gaggtcatca
      721 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
      781 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      841 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
      901 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
      961 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     1021 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     1081 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     1141 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     1201 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     1261 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     1321 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca
     1381 ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg
     1441 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
     1501 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     1561 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
     1621 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     1681 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     1741 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     1801 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     1861 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     1921 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     1981 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     2041 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg
     2101 gcatggacga gctgtacaag gcggccgcaa tggactacaa ggatcatgac ggtgactaca
     2161 aagaccatga tatagattac aaggatgacg acgacaagat ggatacattt gacgtttctg
     2221 ataggaacag ctggtacttt ggtcccatgt ctagacagga tgctactgaa gttttgatga
     2281 acgaacgcga gcggggagtg tttttagtcc gtgatagtaa ctcgatagca ggggattatg
     2341 tactttgtgt aagagaagat acaaaagtta gcaactacat cattaacaaa gttcaacaac
     2401 aggatcaaat cgtttaccgc attggggatc agtcttttga caatctaccg aaactcttaa
     2461 ctttttacac tcttcattat ttggatacaa cccctttaaa acggcctgcg tgtagaaggg
     2521 tggaaaaagt aataggaaag ttcgatttcg ttggcagcga tcaagatgat ttaccttttc
     2581 aaagaggtga agttttaaca atagttcgaa aagacgagga tcaatggtgg actgcgcgta
     2641 actcctcggg gaaaattggt caaataccgg ttccctatat acaacagtat gacgattata
     2701 tggatgaaga tgctattgat aaaaacgaac cttccatttc gggatctagc aatgtatttg
     2761 aaagtactct taaaaggaca gatttaaatc gaaaactacc tgcatacgcc cgcgtaaaac
     2821 agtcaagggt ccctaacgca tacgataaga ctgcattaaa attggaaata ggtgacatta
     2881 ttaaagtcac taaaacaaac attaatgggc aatgggaggg agaattaaat ggaaaaaatg
     2941 gtcattttcc cttcacgcac gttgaatttg tcgatgattg tgatttaagc aaaaactcca
     3001 cagaaatatg ctaagcatgc atctagagtc gacctcgaac gttaacgtta acgtaacgtt
     3061 aactcgagga gcttgataac attataccta aacccatggt caagagtaaa catttctgcc
     3121 tttgaagttg agaacacaat taagcatccc ctggttaaac ctgacattca tacttgttaa
     3181 tagcgccata aacatagcac caattcgaag aatacttgtt aatagcgcca taaacatagc
     3241 accaatttcg aagaaactca gttaaaagca attagcaatt agcaattagc aataactctg
     3301 ctgacttcaa aacgagaaga gttgcaagta tttgtaaggc acagtttata gaccaccgac
     3361 ggctcattag ggctcgtcat gtaactaagc gcggtgaaac ccaattgaac atatagtgga
     3421 attattatta tcaatgggga agatttaacc ctcaggtagc aaagtaattt aattgcaaat
     3481 agagagtcct aagactaaat aatatattta aaaatctggc cctttgacct tgcttgtcag
     3541 gtgcatttgg gttcaatcgt aagttgcttc tatataaaca ctttccccat ccccgcaata
     3601 atgaagaata ccgcagaata aagagagatt tgcaacaaaa aataaaggca ttgcgaaaac
     3661 tttttatggg ggatcattac actcgggcct acggttacaa ttcccagcca cttaagcgac
     3721 aagtttggcc aacaatccat ctaatagcta atagcgcaat cactggtaat cgcaagagta
     3781 tataggcaat agaacccatg gatttgacca aaggtaaccg agacaatgga gaagcaagag
     3841 gatttcaaac tgaacaccca cagtgctgtg tactaccact ggcgcgtttg ggagctcact
     3901 ggcctgatgc gtcctccggg cgtttcaagc ctgctttacg tggtatactc cattacggtc
     3961 aacttggtgg tcaccgtgct gtttcccttg agcttgctgg ccaggctgct gttcaccacc
     4021 aacatggccg gattgtgcga gaacctgacc ataactatta ccgatattgt ggccaatttg
     4081 aagtttgcga atgtgtacat ggtgaggaag cagctccatg agattcgctc tctcctaagg
     4141 ctcatggacg ctagagcccg gctggtgggc gatcccgagg agatttctgc cttgaggaag
     4201 gaagtgaata tcgcacaggg cactttccgc acctttgcca gtattttcgt atttggcact
     4261 actttgagtt gcgtccgcgt ggtcgttcgc ccggatcgag agctcctgta tccggcctgg
     4321 ttcggcgttg actggatgca ctccaccaga aactatgtgc tcatcaatat ctaccagctc
     4381 ttcggcttga tagtgcaggc tatacagaac tgcgctagtg actcctatcc gcctgcgttt
     4441 ctctgcctgc tcacgggtca tatgcgtgct ttggagctga gggtgcggcg gattggcgtc
     4501 gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc
     4561 cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg
     4621 gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg
     4681 cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg acgatgtagg tcacggcggg
     4741 cagccaagct ttgcgtactc gcaaattatt aaaaataaaa ctttaaaaat aatttcgtct
     4801 aattaatatt atgagttaat tcaaacccca cggacatgct aagggttaat caacaatcat
     4861 atcgctgtct cactcagact caatacgaca ctcagaatac tattcctttc actcgcactt
     4921 attgcaagca tacgttaagt ggatgtctct tgccgacggg accaccttat gttatttcat
     4981 catggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt
     5041 cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt tttttttttt
     5101 accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat
     5161 ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc
     5221 ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac
     5281 atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata tacatacttt
     5341 tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt
     5401 tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg
     5461 actgtcgtta gttccgcgcg attcgggtgc ggtatttcac accgcatatg gtgcactctc
     5521 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5581 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5641 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     5701 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5761 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5821 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5881 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5941 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6001 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6061 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6121 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6181 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6241 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6301 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6361 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6421 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6481 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6541 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6601 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6661 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6721 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6781 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6841 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6901 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6961 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7021 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7081 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7141 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7201 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7261 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7321 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7381 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7441 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7501 agcctatgga aaaacgcctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg
     7561 ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg
     7621 gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc
     7681 aaggtcatcc tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt
     7741 ctggcccatg atgaaataac ataaggtggt cccgtcgata gccgaagctt accgaagtat
     7801 acacttaaat tcagtgcacg tttgcttgtt gagaggaaag gttgtgtgcg gacgaatttt
     7861 tttttgaaaa cattaaccct tacgtggaat aaaaaaaaat gaaatattgc aaattttgct
     7921 gcaaagctgt gactggagta aaattaattc acgtgccgaa gtgtgctatt aagagaaaat
     7981 tgtgggagca gagccttggg tgcagccttg gtgaaaactc ccaaatttgt gatacccact
     8041 ttaatgattc gcagtggaag gctgcacctg caaaaggtca gacatttaaa aggaggcgac
     8101 tcaacgcaga tgccgtacct agtaaagtga tagagcctga accagaaaag ataaaagaag
     8161 gctataccag tgggagtaca caaacagagt aagtttgaat agtaaaaaaa atcatttatg
     8221 taaacaataa cgtgactgtg cgttaggtcc tgttcattgt ttaatgaaaa taagagcttg
     8281 agggaaaaaa ttcgtacttt ggagtacgaa atgcgtcgtt tagagcagca gccgaattaa
     8341 ttctagttcc agtgaaatcc aagcattttc taaattaaat gtattcttat tattatagtt
     8401 gttatttttg atatatataa acaacactat tatgcccacc atttttttga gatgcatcta
     8461 cacaaggaac aaacactgga tgtcactttc agttcaaatt gtaacgctaa tcactccgaa
     8521 caggtcacaa aaaattacct taaaaagtca taatattaaa ttagaataaa tatagctgtg
     8581 agggaaatat atacaaatat attggagcaa ataaattgta catacaaata tttattacta
     8641 atttctattg agacgaaatg aaccactcgg aaccatttga gcgaaccgaa tcgcgcggaa
     8701 ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg
     8761 ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc
     8821 gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg tgttttatgt agcgataaac
     8881 gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg gttagaagac aaatccaaag
     8941 tctacttgtg gggatgttcg aaggggaaat acttgtattc tataggtcat atcttgtttt
     9001 tattggcaca aatataatta cattagcttt ttgagggggc aataaacagt aaacacgatg
     9061 gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg ctactccttg
     9121 cgtcgggccc gaagtcttag agccagatat gcgagcaccc ggaagctcac gatgagaatg
     9181 gccagaccca cgtagtccag cggcagatcg gcggcggaga agttaagcgt ctccaggatg
     9241 accttgcccg aactggggca cgtggtgttc gacgatgtgc agctaatttc gcccggctcc
     9301 acgtccgccc attggttaat cagcagaccc tcgttggcgt aacggaacca tgagaggtac
     9361 gacaaccatt tgaggtatac tggcaccgag cccgagttca agaagaagcc gccaaagagc
     9421 aggaatggta tgataaccgg cggacccaca gacagcgcca tcgaggtcga ggagctggcg
     9481 caggatatta gatatccgaa ggacgttgac acattggcca ccagagtgac cagcgccagg
     9541 cagttgaaga agtgcagcac tccggcccgc agtccgatca tcggataggc aatcgccgtg
     9601 aagaccagtg gcactgtgag aaaaagcggc aattcggcaa tcgttttgcc cagaaagtat
     9661 gtgtcacagc gataaagtcg acttcgggcc tccctcataa aaactggcag ctctgaggtg
     9721 aacacctaaa tcgaatcgat tcattagaaa gttagtaaat tattgaaatg caaatgtatt
     9781 ctaaacatga cttacattta tcgtggcaaa gacgttttga aaggtcatgt tggtcaggaa
     9841 gaggaagatg gctccgttga tattcatcac acccacttgc gtgagttgtt ggcccaaaaa
     9901 gatgaggcca atcaagatgg caaccatctg caaattaaaa tgttactcgc atctcattaa
     9961 tattcgcgag ttaaatgaaa tttatttatc ttctgcaaaa ctataaacta tacatctcat
    10021 tgaaaaaaac taagaagggt gtggaatcag gcaattctat ctaaaatcta gcgaatttgt
    10081 ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac cgttgtctga
    10141 ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga tcgccacagg
    10201 accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc cggctgctcc
    10261 agtggcttct ccagattttt ggtggccaac aactgctcca tatcccgggc tactttgcta
    10321 atggcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc ccgtccgggc
    10381 acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg acactgggca
    10441 cccacgctgg ataggagttg agatgttatg taatactaga tacccttaat aaacacatcg
    10501 aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag ctaccctgcc
    10561 ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg gctgatgaat
    10621 ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga cgacgctgtg
    10681 ggcggtaaag gagtccagtc cggaggtggg ctcatcgcag atcagaagcg gcggatcggt
    10741 tagagcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac ctttcaccct
    10801 gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct ggatcacctg
    10861 atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca ccatggcttg
    10921 gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct gctggacata
    10981 ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca gtcgcatccc
    11041 ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca gcagggtcgt
    11101 ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg ccacgccgca
    11161 aactgagttt caaattggta attggaccct ttattaagat ttcacacaga tcagccgact
    11221 gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat gtgtcgctcg
    11281 ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc cggctgattg
    11341 accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc tagttggccg
    11401 ctccctgaac cggagtcctc cggcggactg ggtggccgga gcgtgccgta gtttttggcc
    11461 tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc ctgcaatgat
    11521 aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac aaaaagtacc
    11581 ccgaagtatc ctacgaagta ggtttatact tttatttatt ttttgtgcat ctaggatcag
    11641 cttaaaatat ctggttgtta tattttttgt aaaaaagaat atagtcgaaa atgaatgcct
    11701 ttagatgtct tgatcatgat atgatctcaa aaattgtctt atatagcgag aacagctacc
    11761 agaataatct gtttcgtgtc actatttgtt tgtgcaattg cggtttggga tttttgtggg
    11821 tcgcagttct cacgccgcag acaatttgat gttgcaatcg cagttcctat agatcaagtg
    11881 aacttaagat gtatgcacat gtactactca cattgttcag atgctcggca gatgggtgtt
    11941 tgctgcctcc gcgaattaat agctcctgat cctcttggcc cattgccggg atttttcaca
    12001 ctttcccctg cttacccacc caaaaccaat caccacccca atcactcaaa aaacaaacaa
    12061 aaataagaag cgagaggagt tttggcacag cactttgtgt ttaattgatg gcgtaaaccg
    12121 cttggagctt cgtcacgaaa ccgctgacaa aatgcaactg aaggcggaca ttgacgctac
    12181 gtaacgctac aaacggtggc gaaagagata gcggacgcag cggcgaaaga gacggcgata
    12241 tttctgtgga cagagaagga ggcaaacagc gctgactttg agtggaatgt cattttgagt
    12301 gagaggtaat cgaaagaacc tggtacatca aatacccttg gatcgaagta aatttaaaac
    12361 tgatcagata agttcaatga tatccagtgc agtaaaaaaa aaaaatgttt tttttatcta
    12421 ctttccgcaa aaatgggttt tattaactta catacatact ag
//
