LOCUS       Exported                5788 bp ds-DNA     circular SYN 25-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
COMMENT     >pDsRed-attP sequence 4098 bps
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    373..1187
                     /label=Upstream HR
     misc_feature    1188..1237
                     /label=attP (short)
     misc_feature    1238..1271
                     /label=loxP
                     /label=loxP(1)
     misc_feature    1503..2180
                     /label=dsRed
     misc_feature    2429..2462
                     /label=loxP
     misc_feature    2463..3462
                     /label=downstream HR
     misc_feature    4928..5788
                     /label=AmpR
ORIGIN
        1 attcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc
      121 aattctgaac attatcgcga gcccatttat acctgaatat ggctcataac accccttgtt
      181 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
      241 gccgtagcgc cgatggtagt gtggggactc cccatgcgag agtagggaac tgccaggcat
      301 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gcccgggcta attatggggt
      361 gtcgcccttc gccttgagca tgcaaaggaa tggctattac aatataattt gattagtatg
      421 ctacgcaaac ctctagcatt ttgataggaa aaagattcgg acaaagattc gttgtcagtt
      481 ttttgaagaa gatgaagagg cggatggtgc agtggtctgg ccatgtgagg aaagtttatt
      541 tcccacgggc ccctttttcc tatttttgat cctagcttca aaatcttata tatcttatgt
      601 ccatagcgta agataagtta aatttttcca cttttatatt acttttatat tatcgacttt
      661 tgttttgtct tgcatataag accatacatc agatgatttt tgatcaggtg aaaaccgaga
      721 aacgaaagtt tgtttcttaa gtggaatcac cgcgagtggt tttggccctg cagattgtat
      781 ttctgaagtg ccaacttgtg ccgggagtcc ggactcaatg ttcccttgtg gtggtaaact
      841 aatcgggaca ggtagaatca ctggttaaga aaccagtgca gacaattcgg aatctttagc
      901 aatcccaggg tgggctccct ccacagcgga ttgatcggat tgctccaaat cttatttagc
      961 tgccgatctt agcaacatat gcggattttc tacgattgac agctgatcag ttgtggagtc
     1021 aaaataacat gtcaaccaga tatattcgta ttcctaatat tcctgtaaat atccttgaat
     1081 tgaacagaag aacaaatata tcggttttac ttaaaaatta gaatatcaaa ttgatatgca
     1141 ttgatcacgg ttttacattt agattttaaa agctgacagt acgtcctgta gtgccccaac
     1201 tggggtaacc tttgagttct ctcagttggg ggcgtagata acttcgtata atgtatgcta
     1261 tacgaagtta tcgtacggga tctaattcaa ttagagacta attcaattag agctaattca
     1321 attaggatcc aagcttatcg atttcgaacc ctcgaccgcc ggagtataaa tagaggcgct
     1381 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
     1441 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat cggctcgaag ccggtcgcca
     1501 ccatggcctc ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg
     1561 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     1621 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     1681 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc
     1741 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     1801 aggacggcgg cgtggtgacc gtgacccagg actcctccct ccaggacggc tccttcatct
     1861 acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtaatg cagaagaaga
     1921 ctatgggctg ggaggcgtcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     1981 agatccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca
     2041 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac tccaagctgg
     2101 acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc gccgagggcc
     2161 gccaccacct gttcctgtag gggccgcgac tctagatcat aatcagccat accacatttg
     2221 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2281 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2341 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2401 ccaaactcat caatgtatct taaccggtat aacttcgtat aatgtatgct atacgaagtt
     2461 attgctacag aaaacccttt gggccttata ttttttatac ccgttactcg tagagtaaaa
     2521 gggaatacta caataggttc gttgaaaagt atgtaacagg tggaagaaag cgcttctgac
     2581 catataaagg ctgttcaata tacaaagacc ccaaaagccg gatacatctc cgagtaacgc
     2641 cagaacgcca gaagtttttt tatcacccgc aaagaggtcc ttatttggcc cttcgaccac
     2701 cttccaaaat ttgatatttc caaacgtcca taattcgagg ctaacagaac ctacctctaa
     2761 ggcaccaact ctacaagctg cccaaactaa tttgacgtcg aatccttaat caccgacgca
     2821 tcaccaacgc agtattaaaa ctatgacata tactttgcag catcatatga tttcattcat
     2881 gtcgttcgtg tggattatca cgcaagacct atcttcctgg aacgttaaaa taatgtgtat
     2941 ctttagatca atagttagtt agtgcttctc ctgataccac gttccgagag cacctagagt
     3001 acgcccacaa aaaggccagc gctactactg gttctctgcc gagaatgctt ctaaacacga
     3061 gaggtcctaa gcaaaccact agctaccacg ttgataacgg gcgacaaagc atttttgggc
     3121 ggctgaaggg cgttagagtg gccgtggcaa aaagtttttt gggaaatcga tagaaaatta
     3181 caagactaat caaaatattt aaagaatatc aacacacttt ttcaaaagtg cgggcgtggc
     3241 agttttgcgc ggtttgtagg cgttagagtg ggcgtggcaa catgggtcaa cgctgcgctg
     3301 cgtcttttct agattctgta tgctgaatct catccttcta gcttttatag ttcctgtaat
     3361 cgtgacgttc atacggacag acggacatga ccagatcgtc tcggctattg atcctgatca
     3421 ggaatggcaa catgggtcaa caaacttgtg ctgtctatgt tcgtcaatcg agttcaaggg
     3481 cgacacaaaa tttattctaa atgcataata aatactgata acatcttata gtttgtatta
     3541 tattttgtat tatcgttgac atgtataatt ttgatatcaa aaactgattt tccctttatt
     3601 attttcgaga tttattttct taattctctt taacaaacta gaaatattgt atatacaaaa
     3661 aatcataaat aatagatgaa tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt
     3721 atcttattta aagtgcgttg cttttttctc atttataagg ttaaataatt ctcatatatc
     3781 aagcaaagtg acaggcgccc ttaaatattc tgacaaatgc tctttcccta aactcccccc
     3841 ataaaaaaac ccgccgaagc gggtttttac gttatttgcg gattaacgat tactcgttat
     3901 cagaaccgcc cagggggccc gagcttaaga ctggccgtcg ttttacaaca cagaaagagt
     3961 ttgtagaaac gcaaaaaggc catccgtcag gggccttctg cttagtttga tgcctggcag
     4021 ttccctactc tcgccttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4081 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4141 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4201 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4261 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4321 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4381 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4441 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4501 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4561 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4621 cttgaagtgg tgggctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4681 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4741 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4801 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgcgcgt
     4861 aactcacgtt aagggatttt ggtcatgagc ttgcgccgtc ccgtcaagtc agcgtaatgc
     4921 tctgctttta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4981 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5041 ctggccccag cgctgcgatg ataccgcgag aaccacgctc accggctccg gatttatcag
     5101 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5161 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5221 tgcgcaacgt tgttgccatc gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5281 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5341 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5401 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5461 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5521 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5581 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5641 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5701 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5761 gggcgacacg gaaatgttga atactcat
//
