LOCUS       Exported               10364 bp ds-DNA     circular SYN 08-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 8, 2019 from SnapGene 5.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10364
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..9
                     /label=M13-rev
                     /note="color: #85dae9; direction: RIGHT"
     promoter        300..709
                     /label=S. cerevisiae LEU2
                     /note="color: #85dae9"
     promoter        300..709
                     /label=LEU2 promoter
                     /note="color: #c7b0e3"
     CDS             710..724
                     /label=3-isopropylmalate dehydrogenase, required for  leu
                     /note="color: #d59687; direction: RIGHT"
     promoter        750..1249
                     /label=Sup35 promoter
                     /note="color: #85dae9"
     protein_bind    1262..1386
                     /label=attR1
                     /note="color: #d59687"
     promoter        1411..1441
                     /label=lac UV5 promoter
                     /note="color: #ffef86"
     CDS             1495..2154
                     /label=CmR
                     /note="color: #85dae9; direction: RIGHT"
     primer_bind     complement(1561..1580)
                     /label=CAT-R
                     /note="color: #c7b0e3; direction: LEFT"
     CDS             2496..2801
                     /label=ccdB
                     /note="color: #84b0dc; direction: RIGHT"
     primer_bind     2680..2699
                     /label=ccdB-fwd
                     /note="color: #f58a5e; direction: RIGHT"
     protein_bind    complement(2842..2966)
                     /label=attR2
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    2959..2997
                     /label=Space between attR1 site
                     /note="color: #804000"
     CDS             2998..3042
                     /label=NLS
                     /note="color: #d59687; direction: RIGHT"
     CDS             3004..3024
                     /label=SV40 NLS
                     /note="color: #d6b295; direction: RIGHT"
     CDS             3055..3288
                     /label=VP16 Activation Domain
                     /note="color: #d59687; direction: RIGHT"
     CDS             3310..3561
                     /label=ZF 37-12
                     /note="color: #d59687; direction: RIGHT"
     CDS             3577..3606
                     /label=NES
                     /note="color: #d59687; direction: RIGHT"
     CDS             3628..3819
                     /label=6x HA
                     /note="color: #d59687; direction: RIGHT"
     terminator      3839..4077
                     /label=Sup35 Terminator
                     /note="color: #b90746"
     protein_bind    4236..4246
                     /label=37-12 operator
                     /note="color: #ffef86"
     misc_feature    4237..4245
                     /label=37-12 ZF operator
                     /note="color: #85dae9"
     protein_bind    4255..4265
                     /label=37-12 operator
                     /note="color: #ffef86"
     misc_feature    4256..4264
                     /label=37-12 ZF operator
                     /note="color: #85dae9"
     protein_bind    4276..4286
                     /label=37-12 operator
                     /note="color: #ffef86"
     misc_feature    4277..4285
                     /label=37-12 ZF operator
                     /note="color: #85dae9"
     protein_bind    4295..4305
                     /label=37-12 operator
                     /note="color: #ffef86"
     misc_feature    4296..4304
                     /label=37-12 ZF operator
                     /note="color: #85dae9"
     protein_bind    4315..4325
                     /label=37-12 operator
                     /note="color: #ffef86"
     misc_feature    4316..4324
                     /label=37-12 ZF operator
                     /note="color: #85dae9"
     protein_bind    4334..4344
                     /label=37-12 operator
                     /note="color: #ffef86"
     misc_feature    4335..4343
                     /label=37-12 ZF operator
                     /note="color: #85dae9"
     protein_bind    4352..4362
                     /label=37-12 operator
                     /note="color: #ffef86"
     misc_feature    4353..4361
                     /label=37-12 ZF operator
                     /note="color: #85dae9"
     protein_bind    4371..4381
                     /label=37-12 operator
                     /note="color: #ffef86"
     misc_feature    4372..4380
                     /label=37-12 ZF operator
                     /note="color: #85dae9"
     promoter        4391..4624
                     /label=Minimal CYC1 promoter
                     /note="color: #85dae9"
     CDS             4625..4637
                     /label=ORF1
                     /note="color: #d59687; direction: RIGHT"
     CDS             4638..5336
                     /label=mNeonGreen
                     /note="color: #b1ff67; direction: RIGHT"
     primer_bind     4638..4661
                     /label=DML_P218_F
                     /note="color: #ffef86; direction: RIGHT"
     regulatory      4884..4893
                     /note="/label=vertebrate consensus sequence for strong 
                     initiation of translation (Kozak, 1987)"
                     /note="color: #f58a5e"
     primer_bind     complement(5315..5336)
                     /label=DML_P219_R
                     /note="color: #ffef86; direction: LEFT"
     primer_bind     5316..5336
                     /label=EGFP-type terminus
                     /note="color: #ffef86; direction: RIGHT"
     terminator      5360..5547
                     /label=ADH1 terminator
                     /note="color: #c7b0e3"
     terminator      5360..5539
                     /label=yADH1_terminator
                     /note="color: #b90746"
     primer_bind     complement(5415..5439)
                     /label=pHybLex-R
                     /note="color: #9eafd2; direction: LEFT"
     misc_feature    5772..5791
                     /label=kanB
                     /note="color: #804000"
     CDS             5971..6780
                     /label=KanR
                     /note="color: #ffef86; direction: RIGHT"
     CDS             5971..6777
                     /label=KanR2(variant)
                     /note="color: #d59687; direction: RIGHT"
     primer_bind     complement(6038..6057)
                     /label=Kan-R
                     /note="color: #84b0dc; direction: LEFT"
     misc_feature    6810..7288
                     /label=Leu2 terminator homology
                     /note="color: #804000"
     primer_bind     7297..7313
                     /label=pBluescriptSK
                     /note="color: #faac61; direction: RIGHT"
     primer_bind     7297..7313
                     /label=SK primer
                     /note="color: #85dae9; direction: RIGHT"
     primer_bind     complement(7327..7354)
                     /label=T7
                     /note="color: #85dae9; direction: LEFT"
     promoter        complement(7328..7346)
                     /label=T7 promoter
                     /note="color: #ffef86; direction: LEFT"
     primer_bind     complement(7353..7370)
                     /label=M13 Forward
                     /note="color: #85dae9; direction: LEFT"
     primer_bind     complement(7353..7369)
                     /label=M13 fwd
                     /note="color: #b7e6d7; direction: LEFT"
     primer_bind     complement(7362..7384)
                     /label=M13/pUC Forward
                     /note="color: #b4abac; direction: LEFT"
     CDS             7441..7509
                     /label=LacZ alpha
                     /note="color: #84b0dc; direction: RIGHT"
     primer_bind     complement(7669..7688)
                     /label=F1ori-R
                     /note="color: #c7b0e3; direction: LEFT"
     primer_bind     7801..7822
                     /label=F1ori-F
                     /note="color: #d6b295; direction: RIGHT"
     primer_bind     complement(7976..7995)
                     /label=pRS-marker
                     /note="color: #f8d3a9; direction: LEFT"
     primer_bind     8095..8117
                     /label=pGEX 3'
                     /note="color: #ff9ccd; direction: RIGHT"
     primer_bind     complement(8155..8173)
                     /label=pBRforEco
                     /note="color: #ff9ccd; direction: LEFT"
     promoter        8241..8345
                     /label=AmpR promoter
                     /note="color: #9eafd2"
     CDS             8346..9206
                     /label=AmpR
                     /note="color: #f8d3a9; direction: RIGHT"
     CDS             8544..9203
                     /label=AmpR
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     complement(8564..8583)
                     /label=Amp-R
                     /note="color: #faac61; direction: LEFT"
     rep_origin      9301..9983
                     /label=ColE1 origin
                     /note="color: #84b0dc"
     rep_origin      9377..9965
                     /label=ori
                     /note="color: #d6b295"
     primer_bind     9866..9885
                     /label=pBR322ori-F
                     /note="color: #ff9ccd; direction: RIGHT"
     primer_bind     10119..10136
                     /label=L4440
                     /note="color: #d59687; direction: RIGHT"
     protein_bind    10253..10274
                     /label=CAP binding site
                     /note="color: #84b0dc"
     misc_binding    10325..10347
                     /label=LacO
                     /note="color: #84b0dc"
     protein_bind    10329..10345
                     /label=lac operator
                     /note="color: #ff9ccd"
     primer_bind     10334..10356
                     /label=M13/pUC Reverse
                     /note="color: #75c6a9; direction: RIGHT"
     primer_bind     join(10353..10364,1..5)
                     /label=M13 rev
                     /note="color: #b7e6d7; direction: RIGHT"
     primer_bind     join(10353..10364,1..5)
                     /label=M13 Reverse
                     /note="color: #faac61; direction: RIGHT"
     primer_bind     10353..10364
                     /label=M13-rev
                     /note="color: #85dae9; direction: RIGHT"
ORIGIN
        1 atgaccatga ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc
       61 cgaggagaac ttctagtata tctacatacc taatattatt gccttattaa aaatggaatc
      121 ccaacgatta catcaaaatc cacattctct tcaaaatcaa ttgtcctgta cttccttgtt
      181 catgtgtgtt caaaaacgtt atatttatag gataattata ctctatttct caacaagtaa
      241 ttggttgttt ggccgagcgg tctaaggcgc ctgattcaag aaatatcttg accgcagtta
      301 actgtgggaa tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat
      361 ttttttcctc aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg
      421 actggaaatt ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa
      481 aattgggaga aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa
      541 gcgttcatga ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc
      601 tattgttttt tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt
      661 acatttcagc aatatatata tatatatntt tcaaggatat accattctaa tgtctgcccc
      721 taagatggcg tcgtacgctg caccgagtgc aaccacacaa aaatcataca acgaatggta
      781 tatgcttcat ttctttgttt cgcattagct gcgctatttg actcaaatta ttatttttta
      841 ctaagacgac gcgtcacagt gttcgagtct gtgtcatttc ttttgtaatt ctcttaaacc
      901 acttcataaa gttgtgaagt tcatagcaaa attcttccgc aaaaagatga atcttagttc
      961 tcagcccacc aaaagaggta catgctaaga tcatacagaa gttattgtca cttcttacct
     1021 tgctcttaaa tgtacattac aaccgggtat tatatcttac atcatcgtat aatatgatct
     1081 ttctttatgg agaaaatttt tttttcactc gaccaaagct cccattgctt ctgaagagtg
     1141 tagtgtatat tggtacatct tctcttgaaa gactccattg tactgtaaca aaaagcggtt
     1201 tcttcatcga cttgctcgga ataacatcta tatctgccca ctagcaacag ctagccccat
     1261 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     1321 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     1381 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
     1441 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
     1501 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     1561 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     1621 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     1681 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
     1741 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     1801 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     1861 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     1921 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     1981 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     2041 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     2101 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcgt
     2161 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt
     2221 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc
     2281 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt
     2341 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga
     2401 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa
     2461 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata
     2521 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg
     2581 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg
     2641 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
     2701 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
     2761 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac
     2821 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta
     2881 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     2941 cgttcagctt tcttgtacaa agtggtgatg ggctgcagga attcgatatc aagcttaatg
     3001 gctccaaaga agaagagaaa ggttggtatt catggtgttc cagttgatgg tggtttacaa
     3061 gctccaccaa ctgatgtttc tttgggtgat gaattgcatt tggatggtga agatgttgct
     3121 atggctcatg ctgatgcttt ggatgatttt gatttggata tgttgggtga cggtgattct
     3181 ccaggtccag gttttactcc acatgattct gctccatatg gtgctttaga tatggctgac
     3241 tttgaattcg aacaaatgtt caccgatgcc ttgggtattg atgaatatgg tggtggtgct
     3301 agacccgggg agcgcccctt ccagtgtcgc atttgcatgc ggaacttttc gagaaatttc
     3361 attttgcaaa gacatacccg tactcatacc ggtgaaaaac cgtttcagtg tcggatctgt
     3421 atgcgaaatt tctccgatag agctaatttg agaagacatc tacgtacgca caccggcgag
     3481 aagccattcc aatgccgaat atgcatgcgc aacttcagta gacatgatca attgactaga
     3541 cacctaaaaa cccacctgag aggttctgcc ggcgaattgg ctttgaaatt ggctggtttg
     3601 gatatcaatt ctggttctgc tgctagatac ccatatgatg ttccagatta tgctggttac
     3661 ccttacgatg ttcctgacta cgcaggttct tatccatacg atgtacctga ttatgcctca
     3721 agatatcctt atgacgtccc tgattacgcc ggttatccat atgacgtacc agactacgct
     3781 ggtagttacc catacgacgt cccagattac gcttcttcat aatgagggcc ctcatgtaat
     3841 ttcttgcaaa cataagtaaa tgcaaacaca ataataccga tcataaagca ttttcttcta
     3901 tattaaaaaa caaggtttaa taaagctgtt atatatatat atatatatat agacgtataa
     3961 ttagtttagt tctttttgta ccatatacca taaacaaggt aaacttcacc tctcaatata
     4021 tctagaattt cataaaaata tctagcaagg tttcaactcc ttcaatcacg ttttcatcac
     4081 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4141 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     4201 ttacaatttc ctgatgcggt attttcttta attaatgagg acgtgtagtt taattgagga
     4261 cgtgtggttc taaagtgagg acgtgtatta aatatgagga cgtgtttaaa gcattgagga
     4321 cgtgtcctaa atctgaggac gtgtaattat ctgaggacgt gttttagaag ttaggacgtg
     4381 tggccggccc agatccgcca ggcgtgtata tatagcgtgg atggccaggc aactttagtg
     4441 ctgacacata caggcatata tatatgtgtg cgacgacaca tgatcatatg gcatgcatgt
     4501 gctctgtatg tatataaaac tcttgttttc ttcttttctc taaatattct ttccttatac
     4561 attaggacct ttgcagcata aattactata cttctataga cacacaaaca caaatacaca
     4621 cactgtttaa acaaaaaatg ggcgaggagg ataacatggc ctctctccca gcgacacatg
     4681 agttacacat ctttggctcc atcaacggtg tggactttga catggtgggt cagggcaccg
     4741 gcaatccaaa tgatggttat gaggagttaa acctgaagtc caccaagggt gacctccagt
     4801 tctccccctg gattctggtc cctcatatcg ggtatggctt ccatcagtac ctgccctacc
     4861 ctgacgggat gtcgcctttc caggccgcca tggtagatgg ctccggatac caagtccatc
     4921 gcacaatgca gtttgaagat ggtgcctccc ttactgttaa ctaccgctac acctacgagg
     4981 gaagccacat caaaggagag gcccaggtga aggggactgg tttccctgct gacggtcctg
     5041 tgatgaccaa ctcgctgacc gctgcggact ggtgcaggtc gaagaagact taccccaacg
     5101 acaaaaccat catcagtacc tttaagtgga gttacaccac tggaaatggc aagcgctacc
     5161 ggagcactgc gcggaccacc tacacctttg ccaagccaat ggcggctaac tatctgaaga
     5221 accagccgat gtacgtgttc cgtaagacgg agctcaagca ctccaagacc gagctcaact
     5281 tcaaggagtg gcaaaaggcc tttaccgatg tgatgggcat ggacgagctg tacaagtaag
     5341 gcgcgccact tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt
     5401 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
     5461 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agtatgaggt
     5521 cgctcttatt gaccacacct ctaccggcag atccgctagg gataacaggg taatatagat
     5581 cgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
     5641 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
     5701 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
     5761 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
     5821 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
     5881 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
     5941 catcacatcc gaacatcaat tgaaacaacc atgggtaagg aaaagactca cgtttcgagg
     6001 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     6061 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg
     6121 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     6181 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     6241 gatgcatggt tactcaccac tgcgatcccc ggcaaaacag cattccaggt attagaagaa
     6301 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     6361 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     6421 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     6481 tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca
     6541 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     6601 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     6661 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     6721 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     6781 agatcttcag tactgacaat aaaaagattg cttaaaaaga ttctcttttt ttatgatatt
     6841 tgtacataaa ctttataaat gaaattcata atagaaacga cacgaaatta caaaatggaa
     6901 tatgttcata gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga
     6961 aaaaggagga tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa
     7021 ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt
     7081 taggtgtaac agaaaatcat gaaactatga ttcctaattt atatattgga ggattttctc
     7141 taaaaaaaaa aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt
     7201 taagtcaata cctccttgaa ccatttccca taatggtgaa agttccctca agaattttac
     7261 tctgtcagaa acggccttaa cgacgtaggc ggccgctcta gaactagtgg atcgatcccc
     7321 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     7381 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7441 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7501 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     7561 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7621 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
     7681 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggtg
     7741 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7801 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     7861 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     7921 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     7981 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     8041 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8101 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     8161 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     8221 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8281 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8341 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8401 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8461 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8521 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8581 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8641 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8701 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8761 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8821 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8881 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8941 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9001 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9061 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9121 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9181 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9721 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9781 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9841 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9901 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9961 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10021 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10081 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10141 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10201 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    10261 gttagctcac tcattaggca ccccaggctt tacactttat gcttccgcgg ctcgtatgtt
    10321 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agct
//
