LOCUS       Exported                7050 bp ds-DNA     circular SYN 25-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pACYC-Duet1-Flag-Cdc3-Cdc10-AGT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     operon          3..27
                     /label=lac-Operator-1
     RBS             58..63
                     /label=RBS-1
     primer_bind     60..73
                     /label=GATC-Duet-MCS1-fw-306523
     primer_bind     70..110
                     /label=Cdc3_PscI_for #25
     misc_feature    71..1648
                     /label=Flag-Cdc3
     misc_feature    71..1645
                     /label=Flag-Cdc3
     primer_bind     complement(1627..1654)
                     /label=Cdc3_SalI_rv #2
     primer_bind     1662..1673
                     /label=GATC-DuetUP2
     primer_bind     complement(1692..1709)
                     /label=GATC-Duet-MCS1rv-313351
     primer_bind     1700..1719
                     /label=GATC-DuetUP2
     promoter        1726..1742
                     /label=T7 promotor-2
     operon          1745..1769
                     /label=lac-Operator-2
     RBS             1798..1803
                     /label=RBS-2
     primer_bind     1860..1884
                     /label=Cdc10_KpnI_for (new) #4
     misc_feature    1866..3395
                     /label=Cdc10-AGT
     misc_feature    1866..3392
                     /label=Cdc10-AGT
     primer_bind     complement(3376..3401)
                     /label=AGT_XhoI_rv #21
     primer_bind     complement(3490..3507)
                     /label=pET-RP
     terminator      3504..3539
                     /label=T7 terminator
     misc_feature    3774..4430
                     /label=cat (CmR)
     misc_feature    4792..5704
                     /label=P15A ori
     misc_feature    5831..6910
                     /label=lacI
     promoter        7034..7050
                     /label=T7 promotor-1
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atgttggatt acaaggatag tttaaaggag gaacaagtgt ccattaagca
      121 ggacccggaa caagaagagc gtcagcatga tcaattcaat gatgttcaga tcaaacaaga
      181 gtcgcaggat catgatggag tggactcgca gtacacgaac ggtacgcaaa atgacgacag
      241 cgagagattt gaggcagctg aatccgatgt aaaggtggaa cccggcctcg gtatgggcat
      301 cacctcctct cagagcgaaa agggtcaagt tctgcctgac caaccggaga ttaagttcat
      361 tcgtcgtcaa atcaatggtt atgtagggtt tgctaatttg cccaagcaat ggcacagaag
      421 gtccataaag aacggattca gtttcaatct cctatgtgtc ggccctgatg gtattggtaa
      481 gacaacttta atgaaaactt tatttaataa tgatgatatt gaggccaatt tagtgaaaga
      541 ttacgaggag gaacttgcaa atgatcagga agaagaggag ggtcagggcg agggtcacga
      601 gaaccaatca caagaacaaa ggcacaaggt caaaattaag tcatatgagt cggttattga
      661 agaaaatggt gttaaactga atttgaacgt gatcgatact gaaggatttg gtgatttttt
      721 aaataatgat caaaaatcat gggacccgat cattaaggag attgactctc gttttgatca
      781 atacttggat gcggagaaca aaatcaacag acattcaata aacgataaaa gaatccatgc
      841 atgtctttat ttcattgaac ctacaggaca ttacttaaag cccctggacc tgaagtttat
      901 gcaatctgta tatgaaaaat gtaacttgat tcctgtcatt gctaaatctg acatcttaac
      961 tgatgaagag atcttaagtt tcaagaagac cattatgaac cagttaatcc aatctaatat
     1021 cgagctgttc aagcctccaa tttattccaa cgatgatgca gaaaatagcc atttatccga
     1081 aagactattt agtagtttac cttatgctgt tattggttcc aacgatattg tagagaacta
     1141 cagtggtaat caagtgagag gccgttccta tccatggggg gtgatcgaag tggacaatga
     1201 caaccactct gatttcaacc ttttgaaaaa tttattgatc aagcaattca tggaggaatt
     1261 aaaggaaagg acaagcaaga tactatacga aaactacagg tcttctaaat tggcaaaact
     1321 tggtattaag caagataatt cagttttcaa agagttcgat ccaatatcta aacaacaaga
     1381 agaaaaaact ttacatgagg caaaactagc caaactagaa attgaaatga aaacagtttt
     1441 ccaacaaaaa gtttccgaaa aggagaaaaa actacaaaaa tcagaaactg aattgtttgc
     1501 tagacataaa gaaatgaagg aaaaattaac taaacaatta aaagctttgg aggacaagaa
     1561 aaaacagcta gaactttcaa taaatagtgc ttctcccaat gttaaccact cccccgtccc
     1621 tacaaagaag aagggatttt tacgttaggt cgacaagctt gcggccgcat aatgcttaag
     1681 tcgaacagaa agtaatcgta ttgtacacgg ccgcataatc gaaattaata cgactcacta
     1741 taggggaatt gtgagcggat aacaattccc catcttagta tattagttaa gtataagaag
     1801 gagatataca tatggcagat ctcaattgga tatcggccgg ccacgcgatc gctgacgtcg
     1861 gtaccatgga tcctctcagc tcagtacagc ctgcttctta tgttggtttt gataccatca
     1921 cgaatcagat cgaacatcgt ctgttgaaga aaggttttca atttaatata atggttgttg
     1981 gccaatccgg attgggtaaa agtactctaa taaatacgtt atttgcctca catttgattg
     2041 attctgctac tggtgatgat atttctgccc tgcctgttac aaaaacaact gaaatgaaaa
     2101 tttctactca tactcttgtg gaggaccgcg ttcgcttgaa tattaatgtt atagatacac
     2161 ctggatttgg tgactttatt gacaattcta aagcttggga gcctattgtg aagtacatta
     2221 aggaacaaca ttctcaatac ttacgtaaag aattgacagc ccaacgtgaa aggtttatta
     2281 ctgatacaag agttcatgca attctttatt tcctgcaacc aaatggaaag gagttgagcc
     2341 gccttgacgt tgaagccttg aaaagattga cagaaatagc aaatgttata ccagttattg
     2401 gcaagtcgga tacattgact ttagatgaaa gaacggagtt tagggagctt attcaaaatg
     2461 aattcgaaaa atacaatttc aagatttatc cttatgattc ggaagaacta actgacgagg
     2521 aattagaact aaacagaagt gttagatcta tcattccgtt tgcagtggtt ggttctgaga
     2581 atgagattga aataaacggt gaaaccttca ggggaagaaa aactcgttgg agcgctatta
     2641 atgttgagga tatcaaccag tgtgattttg tatatttaag ggaatttttg attcgaactc
     2701 atctccaaga cttaatcgaa acaacttcct acattcatta tgaagggttc agagcaagac
     2761 aattaattgc cttgaaagaa aatgcgaata gtcgttcctc agctcatatg tctagcaacg
     2821 ccattcaacg tctgtcgact atggacaagg attgtgaaat gaaacgcacc acactggaca
     2881 gccctttggg gaagctggag ctgtctggtt gtgagcaggg tctgcacgaa ataatttttc
     2941 tgggcaaggg gacgtctgca gctgatgccg tggaggtccc agcccccgct gcggttctcg
     3001 gaggtccgga gcccctgatg caggctacag cctggctgaa tgcctatttc caccagcccg
     3061 aggctatcga agagttcccc gtgccggctc ttcaccatcc cgttttccag caagagtcgt
     3121 tcaccagaca ggtgttatgg aagctgctga aggttgtgaa attcggagaa gtgatttctt
     3181 actctcactt agcagccctg gcaggcaacc ccgcagccac ggcagcagtg aagacggcac
     3241 tgagtggcaa tcctgtccct atcctgatcc cgtgccacag agtggtccag ggggatttgg
     3301 atgtgggcgg ttacgagggt ggactggccg tgaaggaatg gcttctggcc catgaaggcc
     3361 accggttggg gaagccaggc ttgggaagat cttaactcga gtctggtaaa gaaaccgctg
     3421 ctgcgaaatt tgaacgccag cacatggact cgtctactag cgcagcttaa ttaacctagg
     3481 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     3541 ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg cttccggtag
     3601 tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac
     3661 cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat
     3721 tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg
     3781 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     3841 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     3901 taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     3961 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     4021 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     4081 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     4141 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     4201 attgccatac ggaactccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     4261 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     4321 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     4381 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     4441 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     4501 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc
     4561 cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc
     4621 gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag
     4681 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg
     4741 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg
     4801 gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg
     4861 gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg
     4921 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
     4981 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     5041 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     5101 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttcccct
     5161 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     5221 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     5281 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
     5341 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
     5401 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
     5461 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
     5521 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
     5581 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga
     5641 tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt
     5701 tgtaattctc atgttagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     5761 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     5821 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     5941 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     6001 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     6061 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagatg tccgcaccaa
     6121 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     6181 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     6241 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     6301 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     6361 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     6421 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     6481 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     6541 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     6601 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     6661 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     6721 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     6781 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     6841 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     6901 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     6961 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     7021 tgcattagga aattaatacg actcactata
//
