LOCUS       Exported                4862 bp ds-DNA     circular SYN 25-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pFA CRU HISMX6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    25..42
                     /label=S3 annealing
     misc_feature    46..168
                     /label=Cub
     misc_feature    169..171
                     /label=R
     misc_feature    181..345
                     /label=lacI
     misc_feature    349..1149
                     /label=URA3 ORF
     misc_feature    1160..2425
                     /label=HIS3MX6
     misc_feature    1160..1538
                     /label=AgTEFprom
     misc_feature    1539..2192
                     /label=SpHIS5
     misc_feature    2194..2425
                     /label=AgTEFterm
     misc_feature    complement(2433..2451)
                     /label=S2 annealing
     misc_feature    complement(3535..4395)
                     /label=ampR
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggtatccc tccagatcaa
       61 caaagattga tctttgccgg taagcagcta gaagacggta gaacgctgtc tgattacaac
      121 attcagaagg agtccacctt acatcttgtg ctaaggctaa gaggtggtag gcacggatcc
      181 ggagcttggc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
      241 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
      301 cgtggctacc catacgacgt cccagattac gccgctttcc ttggccagtc gaaagctaca
      361 tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg
      421 cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg
      481 gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg
      541 actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat
      601 tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac
      661 tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg
      721 ggcccaggta ttgttagcgg tttgaagcag gcggcggaag aagtaacaaa ggaacctaga
      781 ggccttttga tgttagcaga attgtcatgc aagggctccc tagctactgg agaatatact
      841 aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa
      901 agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt
      961 ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct
     1021 acaggatctg acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag
     1081 gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag
     1141 caaaactaaa gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
     1201 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
     1261 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
     1321 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
     1381 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
     1441 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
     1501 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaggagg gcttttgtag
     1561 aaagaaatac gaacgaaacg aaaatcagcg ttgccatcgc tttggacaaa gctcccttac
     1621 ctgaagagtc gaattttatt gatgaactta taacttccaa gcatgcaaac caaaagggag
     1681 aacaagtaat ccaagtagac acgggaattg gattcttgga tcacatgtat catgcactgg
     1741 ctaaacatgc aggctggagc ttacgacttt actcaagagg tgatttaatc atcgatgatc
     1801 atcacactgc agaagatact gctattgcac ttggtattgc attcaagcag gctatgggta
     1861 actttgccgg cgttaaaaga tttggacatg cttattgtcc acttgacgaa gctctttcta
     1921 gaagcgtagt tgacttgtcg ggacggccct atgctgttat cgatttggga ttaaagcgtg
     1981 aaaaggttgg ggaattgtcc tgtgaaatga tccctcactt actatattcc ttttcggtag
     2041 cagctggaat tactttgcat gttacctgct tatatggtag taatgaccat catcgtgctg
     2101 aaagcgcttt taaatctctg gctgttgcca tgcgcgcggc tactagtctt actggaagtt
     2161 ctgaagtccc aagcacgaag ggagtgttgt aaagagtact gacaataaaa agattcttgt
     2221 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa
     2281 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag
     2341 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc
     2401 gattcgatac taacgccgcc atccagttta aacgagctcg aattcatcga tgatatcaga
     2461 tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag tcgtattaat
     2521 ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2581 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2641 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2701 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2761 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2821 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2881 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2941 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     3001 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3061 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3121 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3181 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3241 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3301 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3361 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3421 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3481 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3541 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3601 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3661 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3721 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3781 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3841 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3901 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3961 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4021 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4081 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4141 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4201 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4261 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4321 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4381 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4441 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4501 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4561 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     4621 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     4681 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     4741 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga
     4801 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
     4861 ta
//
