LOCUS       Exported                6186 bp ds-DNA     circular SYN 25-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pExo84-CRU 303
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    503..1162
                     /label=HIS3
     primer_bind     2046..2065
                     /label=T7
     primer_bind     2090..2116
                     /label=Exo84 Cub EagI
     misc_feature    2096..2634
                     /label=Exo84-ORF
     primer_bind     complement(2615..2642)
                     /label=Exo84 Cub SalI
     misc_feature    2647..2769
                     /label=CUb
     misc_feature    2770..2772
                     /label=Arg
     misc_feature    2782..2946
                     /label=lacI
     misc_feature    2950..3750
                     /label=URA3 ORF
     misc_feature    complement(5136..5996)
                     /label=ampR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga
      241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt
      301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt
      361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa
      421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca
      481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa
      541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc
      601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat
      661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc
      721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac
      781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctactgg cgcgtggagt
      841 aaaaaggttt ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga
      901 tctttcgaac aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga
      961 tctctcttgc gagatgatcc cgcattttct tgaaagcttt gcagaggcta gcagaattac
     1021 cctccacgtt gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa
     1081 ggctcttgcg gttgccataa gagaagccac ctcgcccaat ggtaccaacg atgttccctc
     1141 caccaaaggt gttcttatgt agtgacaccg attatttaaa gctgcagcat acgatatata
     1201 tacatgtgta tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata
     1261 ctgaagatga caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgctttc
     1321 cttttttctt tttgcttttt cttttttttt ctcttgaact cgacggatca tatgcggtgt
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa ggggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctgat
     2101 gaagagttaa tgttacttaa cctgatatct ttaaaaatcg aacaaagaag agaggccatt
     2161 tctagcaaac tttctcagag cattttatca tcgaatgaaa tagtgcattt gaaaagcggt
     2221 actgaaaata tgattaagtt aggattacca gagcaagctc ttgacctatt cttacagaat
     2281 agatctaatt tcattcaaga tttgattcta cagattggat cagtggacaa tcctacgaac
     2341 tatttgactc aattggctgt tatcaggttc caaaccatta aaaaaacagt ggaggacttc
     2401 caggatattt tcaaagaact cggggctaag atttcttcca tcctagtaga ttggtgtagc
     2461 gacgaagtgg ataatcactt caaattgata gacaagcaat tactgaatga tgaaatgtta
     2521 tccccagggt ccattaagtc atccagaaaa cagatagatg gattgaaggc cgtaggtttg
     2581 gattttgtct acaaattgga cgagtttatc aaaaagaaca gtgataaaat tcgcgggtcg
     2641 accggtatcc ctccagatca acaaagattg atctttgccg gtaagcagct agaagacggt
     2701 agaacgctgt ctgattacaa cattcagaag gagtccacct tacatcttgt gctaaggcta
     2761 agaggtggta ggcacggatc cggagcttgg ctgttgcccg tctcactggt gaaaagaaaa
     2821 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     2881 cagctggcac gacaggtttc ccgtggctac ccatacgacg tcccagatta cgccgctttc
     2941 cttggccagt cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct
     3001 gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt
     3061 cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta
     3121 aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag
     3181 gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt
     3241 aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt
     3301 acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcggaa
     3361 gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc
     3421 ctagctactg gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt
     3481 gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg
     3541 attatgacac ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga
     3601 accgtggatg atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt
     3661 gcaaagggaa gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca
     3721 tatttgagaa gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata
     3781 ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccgg gaatctcggt
     3841 cgtaatgatt tctataatga cgaaaaaaaa aaaattggaa agaaaaagct tatcgcgata
     3901 ccgtcgaggg ggggcccggt acccagcttt tgttcccttt agtgagggtt aattccgagc
     3961 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4021 cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa
     4081 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4141 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4201 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4261 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4321 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4381 cataggctcg gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4441 aacccgacag gactataaag ataccaggcg ttcccccctg gaagctccct cgtgcgctct
     4501 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4561 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4621 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4681 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4741 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4801 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4861 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4921 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4981 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5041 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5101 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5161 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactgcc cgtcgtgtag
     5221 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5281 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5341 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5401 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5461 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5521 cgagttacat gatcccccat gttgtgaaaa aaagcggtta gctccttcgg tcctccgatc
     5581 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5641 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5701 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5761 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5821 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5881 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5941 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6001 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6061 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6121 ccacctgacg tcttattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     6181 ttcgtc
//
