LOCUS       Exported                7152 bp ds-DNA     circular SYN 25-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pPMET17p-CCG 316
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    411..1214
                     /label=URA3
     primer_bind     1966..1985
                     /label=T7-Primer
     promoter        2007..2536
                     /label=MET17prom
     misc_feature    2623..3330
                     /label=mCherry
     CDS             3343..3465
                     /label=Cub
     misc_feature    3466..3468
                     /label=Arg
     misc_feature    3478..3639
                     /label=lacI
     CDS             3643..4359
                     /label=GFP(S65T)
     primer_bind     complement(4376..4395)
                     /label=T3-Primer
     primer_bind     complement(4416..4437)
                     /label=preT3
     misc_feature    complement(5578..6438)
                     /label=ampR
     centromere      6570..7087
                     /label=CEN6 ARSH4
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
      421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
      481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
      541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
      601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
      661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
      721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
      781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
      841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
      901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
      961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
     1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     1981 tagggcgaat tggagctcca ccgcggtttc gccatttctt gaatgtattt aaagatattt
     2041 aatgctataa tagacattta aatccaattc ttccaacata caatgggagt ttggccgagt
     2101 ggtttaaggc gtcagattta ggtggattta acctctaaaa tctctgatat cttcggatgc
     2161 aagggttcga atcccttagc tctcattatt ttttgctttt tctcttgagg tcacatgatc
     2221 gcaaaatggc aaatggcacg tgaagctgtc gatattgggg aactgtggtg gttggcaaat
     2281 gactaattaa gttagtcaag gcgccatcct catgaaaact gtgtaacata ataaccgaag
     2341 tgtcgaaaag gtggcacctt gtccaattga acacgctcga tgaaaaaaat aagatatata
     2401 taaggttaag taaagcgtct gttagaaagg aagtttttcc tttttcttgc tctcttgtct
     2461 tttcatctac tatttccttc gtgtaataca gggtcgtcag atacatagat acaattctat
     2521 tacccccatc catacatcta gacggccgct ctagaactag tggatccccc gggctgcagg
     2581 aattcgatat caagcttatc gataccgtcg accggcgcgc caatggtgag caagggcgag
     2641 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     2701 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2761 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     2821 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     2881 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2941 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     3001 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     3061 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     3121 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     3181 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     3241 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     3301 tccaccggcg gcatggacga gctgtacaag ggctcgaccg gtatccctcc agatcaacaa
     3361 agattgatct ttgccggtaa gcagctagaa gacggtagaa cgctgtctga ttacaacatt
     3421 cagaaggagt ccaccttaca tcttgtgcta aggctaagag gtggtaggca cggatccgga
     3481 gcttggctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
     3541 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccgt
     3601 ggctacccat acgacgtccc agattacgcc gctggcgcgc caatgggtaa aggagaagaa
     3661 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     3721 ttttctgtca gtggagaggg tgaaggcgat gcaacatacg gaaaacttac ccttaaattt
     3781 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcacctat
     3841 ggtgttcaat gcttttcaag atacccagat catatgaaac ggcatgactt tttcaagagt
     3901 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
     3961 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     4021 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     4081 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagc caacttcaaa
     4141 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     4201 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     4261 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     4321 gctgggatta cacatggcat ggatgaacta tacaaataag gtacccagct tttgttccct
     4381 ttagtgaggg ttaattccga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4441 ttgttatccg ctcacaattc cacacaacat aggagccgga agcataaagt gtaaagcctg
     4501 gggtgcctaa tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4561 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4621 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4681 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4741 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4801 ggccgcgttg ctggcgtttt tccataggct cggcccccct gacgagcatc acaaaaatcg
     4861 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgttcccccc
     4921 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4981 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     5041 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5101 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5161 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5221 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     5281 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5341 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5401 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5461 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5521 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5581 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5641 tgcctgactg cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5701 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5761 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5821 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5881 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5941 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgaa aaaaagcggt
     6001 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6061 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6121 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6181 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6241 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6301 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6361 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6421 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6481 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6541 gcgcacattt ccccgaaaag tgccacctgg gtccttttca tcacgtgcta taaaaataat
     6601 tataatttaa attttttaat ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat
     6661 taaagaaaaa atagtttttg ttttccgaag atgtaaaaga ctctaggggg atcgccaaca
     6721 aatactacct tttatcttgc tcttcctgct ctcaggtatt aatgccgaat tgtttcatct
     6781 tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt acattttact tatcgttaat
     6841 cgaatgtata tctatttaat ctgcttttct tgtctaataa atatatatgt aaagtacgct
     6901 ttttgttgaa attttttaaa cctttgttta tttttttttc ttcattccgt aactcttcta
     6961 ccttctttat ttactttcta aaatccaaat acaaaacata aaaataaata aacacagagt
     7021 aaattcccaa attattccat cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg
     7081 atccgtccta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     7141 ggccctttcg tc
//
