LOCUS       Exported                4520 bp ds-DNA     circular SYN 25-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCUP1pNuiHA kanMX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    65..82
                     /label=S1 annealing
     misc_feature    complement(83..309)
                     /label=AgTEFterm
     misc_feature    complement(315..1127)
                     /label=kan-ORF
     misc_feature    complement(1128..1506)
                     /label=AgTEFprom
     promoter        1520..1951
                     /label=CUP1prom
     misc_feature    1971..2093
                     /label=Nui
     misc_feature    2094..2123
                     /label=HA tag
     misc_feature    complement(2131..2150)
                     /label=S4 annealing
     misc_feature    complement(3233..4093)
                     /label=ampR
ORIGIN
        1 accttatgta tcatacacat acgatttagg tgacactata gaacgcggcc gccagctgaa
       61 gcttcgtacg ctgcaggtcg acactggatg gcggcgttag tatcgaatcg acagcagtat
      121 agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac ttaacttcgc
      181 atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa
      241 tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa caagaatctt
      301 tttattgtca gtactgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
      361 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
      421 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
      481 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
      541 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
      601 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
      661 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
      721 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
      781 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttgccggg gatcgcagtg
      841 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
      901 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
      961 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     1021 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     1081 ttggaattta atcgcggcct cgaaacgtga gtcttttcct tacccatggt tgtttatgtt
     1141 cggatgtgat gtgagaactg tatcctagca agattttaaa aggaagtata tgaaagaaga
     1201 acctcagtgg caaatcctaa ccttttatat ttctctacag gggcgcggcg tggggacaat
     1261 tcaacgcgtc tgtgagggga gcgtttccct gctcgcaggt ctgcagcgag gagccgtaat
     1321 ttttgcttcg cgccgtgcgg ccatcaaaat gtatggatgc aaatgattat acatggggat
     1381 gtatgggcta aatgtacggg cgacagtcac atcatgcccc tgagctgcgc acgtcaagac
     1441 tgtcaaggag ggtattctgg gcctccatgt cgctggccgg gtgacccggc ggggacgagg
     1501 caagctaaac agatctggac gatcccatta ccgacatttg ggcgctatac gtgcatatgt
     1561 tcatgtatgt atctgtattt aaaacacttt tgtattattt ttcctcatat atgtgtatag
     1621 gtttatacgg atgatttaat tattacttca ccacccttta tttcaggctg atatcttagc
     1681 cttgttacta gttagaaaaa gacatttttg ctgtcagtca ctgtcaagag attcttttgc
     1741 tggcatttct tctagaagca aaaagagcga tgcgtctttt ccgctgaacc gttccagcaa
     1801 aaaagactac caacgcaata tggattgtca gaatcatata aaagagaagc aaataactcc
     1861 ttgtcttgta tcaattgcat tataatatct tcttgttagt gcaatatcat atagaagtca
     1921 tcgaaataga tattaagaaa aacaaactgt aacgaatttc gaccggcggt atgcagattt
     1981 tcgtcaagac tttgaccggt aaaaccataa cattggaagt tgaatcttcc gataccatcg
     2041 acaacgttaa gtcgaaaatt caagacaagg aagggatccc tgggtctggg gcttacccat
     2101 acgatgttcc tgactatgcg ggcgcgccga cgacagagaa ttcatcgatg atatcagatc
     2161 cactagtggc ctatgcggcc gcggatctgc cggtctccct atagtgagtc gtattaattt
     2221 cgataagcca ggttaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2281 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2341 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2401 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2461 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2521 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2581 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2641 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     2701 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2761 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2821 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2881 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     2941 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3001 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3061 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3121 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3181 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3241 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3301 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3361 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3421 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3481 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3541 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3601 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3661 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3721 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3781 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3841 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3901 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3961 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4021 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4081 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4141 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4201 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     4261 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     4321 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     4381 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     4441 atgcggcatc agagcagatt gtactgagag tgcaccatat ggacatattg tcgttagaac
     4501 gcggctacaa ttaatacata
//
