LOCUS       Exported                4418 bp ds-DNA     circular SYN 25-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pNuiHA loxP-nat-CUP1p
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    60..77
                     /label=S1 annealing
     misc_feature    78..200
                     /label=Nui
     misc_feature    201..227
                     /label=HA tag
     misc_feature    234..267
                     /label=loxP
     misc_feature    268..646
                     /label=AgTEFprom
     misc_feature    647..1219
                     /label=nat-ORF
     misc_feature    1220..1539
                     /label=ScADH1term
     promoter        1547..1986
                     /label=CUP1prom
     misc_feature    1987..2020
                     /label=loxP
     misc_feature    complement(2024..2043)
                     /label=S4 annealing
     misc_feature    complement(3126..3986)
                     /label=ampR
ORIGIN
        1 atgtatcata cacatacgat ttaggtgaca ctatagaacg cggccgccag ctgaagcttc
       61 gtacgctgca ggtcgacatg cagattttcg tcaagacttt gaccggtaaa accataacat
      121 tggaagttga atcttccgat accatcgaca acgttaagtc gaaaattcaa gacaaggaag
      181 ggatccctgg gtctggggct tacccatacg atgttcctga ctatgcgaga tccataactt
      241 cgtataatgt atgctatacg aagttatagc ttgcctcgtc cccgccgggt cacccggcca
      301 gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat
      361 gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca
      421 tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg
      481 agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga
      541 gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa
      601 tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatgg gtaccactct
      661 tgacgacacg gcttaccggt accgcaccag tgtcccgggg gacgccgagg ccatcgaggc
      721 actggatggg tccttcacca ccgacaccgt cttccgcgtc accgccaccg gggacggctt
      781 caccctgcgg gaggtgccgg tggacccgcc cctgaccaag gtgttccccg acgacgaatc
      841 ggacgacgaa tcggacgacg gggaggacgg cgacccggac tcccggacgt tcgtcgcgta
      901 cggggacgac ggcgacctgg cgggcttcgt ggtcgtctcg tactccggct ggaaccgccg
      961 gctgaccgtc gaggacatcg aggtcgcccc ggagcaccgg gggcacgggg tcgggcgcgc
     1021 gttgatgggg ctcgcgacgg agttcgcccg cgagcggggc gccgggcacc tctggctgga
     1081 ggtcaccaac gtcaacgcac cggcgatcca cgcgtaccgg cggatggggt tcaccctctg
     1141 cggcctggac accgccctgt acgacggcac cgcctcggac ggcgagcagg cgctctacat
     1201 gagcatgccc tgcccctaat ctcgaggcga atttcttatg atttatgatt tttattatta
     1261 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     1321 gaaaattctt gttcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc
     1381 atgaggtcgc tcttattgac cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg
     1441 ctccccattt cacccaattg tagatatgct aactccagca atgagttgat gaatctcggt
     1501 gtgtatttta tgtcctcaga ggacaacacc tgttgtaatc gagctctagt aagccgatcc
     1561 cattaccgac atttgggcgc tatacgtgca tatgttcatg tatgtatctg tatttaaaac
     1621 acttttgtat tatttttcct catatatgtg tataggttta tacggatgat ttaattatta
     1681 cttcaccacc ctttatttca ggctgatatc ttagccttgt tactagttag aaaaagacat
     1741 ttttgctgtc agtcactgtc aagagattct tttgctggca tttcttctag aagcaaaaag
     1801 agcgatgcgt cttttccgct gaaccgttcc agcaaaaaag actaccaacg caatatggat
     1861 tgtcagaatc atataaaaga gaagcaaata actccttgtc ttgtatcaat tgcattataa
     1921 tatcttcttg ttagtgcaat atcatataga agtcatcgaa atagatatta agaaaaacaa
     1981 actgtaataa cttcgtataa tgtatgctat acgaagttat tgacgacaga gaattcatcg
     2041 atgatatcag atccactagt ggcctatgcg gccgcggatc tgccggtctc cctatagtga
     2101 gtcgtattaa tttcgataag ccaggttaac ctgcattaat gaatcggcca acgcgcgggg
     2161 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2221 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2281 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2341 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2401 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2461 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2521 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     2581 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2641 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2701 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2761 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     2821 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2881 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2941 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3001 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3061 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3121 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3181 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3241 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3301 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3361 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3421 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3481 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3541 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3601 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3661 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3721 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3781 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3841 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3901 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3961 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4021 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4081 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     4141 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     4201 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     4261 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     4321 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatggacata
     4381 ttgtcgttag aacgcggcta caattaatac ataacctt
//
