LOCUS       Exported               11510 bp ds-DNA     circular SYN 26-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 31, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        247..626
                     /label=CMV enhancer
     promoter        628..826
                     /label=CMV promoter
     LTR             844..1024
                     /label=5' LTR (truncated)
     misc_feature    1071..1196
                     /label=HIV-1 Psi
     misc_feature    1689..1922
                     /label=RRE
     misc_feature    2449..2566
                     /label=cPPT/CTS
     promoter        2608..2848
                     /label=U6 promoter
     primer_bind     complement(2789..2808)
                     /label=oJWseq4-U6 RV
     misc_feature    2828..2833
                     /label=TATA box
                     /note="RNA transcription starts 23(+1) bases from the end 
                     of this recognition sequence"
     misc_feature    2857..2876
                     /label=Target
     misc_RNA        2877..2952
                     /label=gRNA scaffold
                     /note="in RNA form binds to Cas9"
     misc_feature    2953..2963
                     /label=Termination sequence
                     /note="RNA polymerase will stop transcription at this 
                     position and fall off"
     primer_bind     3042..3098
                     /label=oJW093 GluA1 C FW donor
     misc_feature    3049..3071
                     /label=Target
                     /note="Not so efficient but oke"
     CDS             3072..3104
                     /codon_start=1
                     /label=Linker
                     /translation="GASGSGSSRSG"
     CDS             3105..3815
                     /codon_start=1
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     misc_feature    3827..3849
                     /label=Target
                     /note="Not so efficient but oke"
     enhancer        3894..3919
                     /label=CMV enhancer
     misc_feature    3943..5144
                     /label=hUBC
     CDS             5206..5910
                     /codon_start=1
                     /label=mCherry
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    5911..6177
                     /label=KASH (serpine 2)
     misc_feature    6202..6790
                     /label=WPRE
     CDS             complement(6673..6684)
                     /codon_start=1
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6793..6809)
                     /label=KS primer
     LTR             7315..7495
                     /label=5' LTR (truncated)
     polyA_signal    7527..7751
                     /label=bGH poly(A) signal
     rep_origin      7797..8225
                     /direction=RIGHT
                     /label=f1 ori
     promoter        8239..8568
                     /label=SV40 promoter
     rep_origin      8419..8554
                     /label=SV40 ori
     promoter        8616..8663
                     /label=EM7 promoter
     CDS             8682..9056
                     /codon_start=1
                     /label=BleoR
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9186..9307
                     /label=SV40 poly(A) signal
     primer_bind     complement(9356..9372)
                     /label=M13 rev
     protein_bind    9380..9396
                     /label=lac operator
     promoter        complement(9404..9434)
                     /label=lac promoter
     protein_bind    9449..9470
                     /label=CAP binding site
     rep_origin      complement(9758..10346)
                     /direction=LEFT
                     /label=ori
     CDS             complement(10517..11377)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11378..11482)
                     /label=AmpR promoter
ORIGIN
        1 attnnntncg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1561 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1621 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1681 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1741 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1801 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1861 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1921 ctggggattt ggggttgctc tggaaaattc atttgcacca ctgctgtgcc ttggaatgct
     1981 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     2041 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     2101 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2161 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     2221 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2281 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2341 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2401 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2461 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2521 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2581 ggacagcaga gatccagttt ggttagtgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccggga gccacaggat tgtaacgttt
     2881 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     2941 ccgagtcggt gcttttttgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     3001 tttttagcgc gtgcgccaat tctgcagaca aatggctcta gaagcttacc cgttacaatc
     3061 ctgtggctcc cggcgctagc ggtagcggct ccagtagatc tggggtgagc aagggcgagg
     3121 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3181 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3241 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     3301 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3361 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3421 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3481 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3541 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     3601 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     3661 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     3721 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3781 ccgccgggat cactctcggc atggacgagc tgtacaagta aggcctcccg ttacaatcct
     3841 gtggctcccc tcgaggatcc taggacgcgt cctaggacgc gtccagaggt acccgttaca
     3901 taacttacgg taaatggcct aacccgtgtc ggctccagat ctggcctccg cgccgggttt
     3961 tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
     4021 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
     4081 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
     4141 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
     4201 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
     4261 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
     4321 atcgctgtga tcgtcacttg gtgagttgcg ggctgctggg ctggccgggg ctttcgtggc
     4381 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
     4441 tccgcgagca aggttgccct gaactggggg ttggggggag cgcacaaaat ggcggctgtt
     4501 cccgagtctt gaatggaaga cgcttgtaag gcgggctgtg aggtcgttga aacaaggtgg
     4561 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     4621 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     4681 actggagaac tcgggtttgt cgtctggttg cgggggcggc agttatgcgg tgccgttggg
     4741 cagtgcaccc gtacctttgg gagcgcgcgc ctcgtcgtgt cgtgacgtca cccgttctgt
     4801 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     4861 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     4921 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     4981 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     5041 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     5101 agactagtaa agtgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     5161 gggctgcagg tcgactctag aggatccccg ggtaccggta tggcggtgag caagggcgag
     5221 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     5281 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     5341 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     5401 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     5461 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     5521 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     5581 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     5641 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     5701 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     5761 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     5821 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     5881 tccaccggcg gcatggacga gctgtacaag tccggactca gatctcgaga ggaggaggag
     5941 gagacagaca gcaggatgcc ccacctcgac agccccggca gctcccagcc gagacgctcc
     6001 ttcctctcaa gggtgatcag ggcagcgcta ccgttgcagc tgcttctgct gctgctgctg
     6061 ctcctggcct gcctgctacc tgcctctgaa gatgactaca gctgcaccca ggccaacaac
     6121 tttgcccgat ccttctaccc catgctgcgg tacaccaacg ggccacctcc cacctaggaa
     6181 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     6241 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6301 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6361 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6421 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6481 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6541 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6601 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6661 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6721 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6781 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
     6841 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     6901 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     6961 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7021 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     7081 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     7141 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     7201 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     7261 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     7321 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7381 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7441 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     7501 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     7561 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     7621 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     7681 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     7741 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     7801 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7861 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7921 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7981 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8041 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8101 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8161 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8221 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     8281 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8341 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     8401 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8461 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     8521 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     8581 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     8641 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     8701 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     8761 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     8821 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     8881 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     8941 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     9001 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     9061 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     9121 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     9181 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9241 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9301 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     9361 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9421 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9481 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9541 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9601 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9661 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9721 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9781 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9841 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9901 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9961 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10021 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10081 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10141 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    10201 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10261 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10321 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10381 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10441 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10501 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10561 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10621 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10681 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10741 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10801 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10861 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10921 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10981 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11041 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11101 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11161 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11221 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11281 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11341 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11401 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11461 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
