LOCUS       Exported               11480 bp ds-DNA     circular SYN 26-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 31, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11480
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        247..626
                     /label=CMV enhancer
     promoter        628..826
                     /label=CMV promoter
     LTR             844..1024
                     /label=5' LTR (truncated)
     misc_feature    1071..1196
                     /label=HIV-1 Psi
     misc_feature    1689..1922
                     /label=RRE
     misc_feature    2449..2566
                     /label=cPPT/CTS
     promoter        2608..2848
                     /label=U6 promoter
     misc_RNA        2857..2875
                     /label=Target sequence
     misc_RNA        2876..2951
                     /label=gRNA scaffold
     misc_feature    2952..2962
                     /label=Termination sequence
     misc_feature    3047..3065
                     /label=Target sequence
                     /note="Note that this target is 19bp!!!"
     misc_feature    3066..3068
                     /label=PAM
     CDS             3078..3794
                     /codon_start=1
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    3798..3816
                     /label=Target sequence
     misc_feature    3817..3819
                     /label=PAM
     enhancer        3864..3889
                     /label=CMV enhancer
     misc_feature    3913..5114
                     /label=hUBC
     misc_feature    3995
                     /label=Extra C
     CDS             5176..5880
                     /codon_start=1
                     /label=mCherry
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             5881..6144
                     /codon_start=1
                     /label=KASH domain (spectrin 2)
                     /translation="SGLRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIRAALPLQLL
                     LLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT"
     misc_feature    6172..6760
                     /label=WPRE
     CDS             complement(6643..6654)
                     /codon_start=1
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6763..6779)
                     /label=KS primer
     LTR             7285..7465
                     /label=5' LTR (truncated)
     polyA_signal    7497..7721
                     /label=bGH poly(A) signal
     rep_origin      7767..8195
                     /direction=RIGHT
                     /label=f1 ori
     promoter        8209..8538
                     /label=SV40 promoter
     rep_origin      8389..8524
                     /label=SV40 ori
     promoter        8586..8633
                     /label=EM7 promoter
     CDS             8652..9026
                     /codon_start=1
                     /label=BleoR
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9156..9277
                     /label=SV40 poly(A) signal
     primer_bind     complement(9326..9342)
                     /label=M13 rev
     protein_bind    9350..9366
                     /label=lac operator
     promoter        complement(9374..9404)
                     /label=lac promoter
     protein_bind    9419..9440
                     /label=CAP binding site
     rep_origin      complement(9728..10316)
                     /direction=LEFT
                     /label=ori
     CDS             complement(10487..11347)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11348..11452)
                     /label=AmpR promoter
ORIGIN
        1 attnnntncg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1561 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1621 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1681 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1741 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1801 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1861 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1921 ctggggattt ggggttgctc tggaaaattc atttgcacca ctgctgtgcc ttggaatgct
     1981 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     2041 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     2101 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2161 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     2221 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2281 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2341 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2401 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2461 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2521 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2581 ggacagcaga gatccagttt ggttagtgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccgctg cgagcaactt cacttgtttt
     2881 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     2941 cgagtcggtg cttttttgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     3001 ttttagcgcg tgcgccaatt ctgcagacaa atggctctag aagcttgctg cgagcaactt
     3061 cacttggggg atcaggcgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3121 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3181 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     3241 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     3301 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3361 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     3421 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     3481 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     3541 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     3601 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3661 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     3721 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3781 agctgtacaa gtaatgtgct gcgagcaact tcacttgggc tcgaggatcc taggacgcgt
     3841 cctaggacgc gtccagaggt acccgttaca taacttacgg taaatggcct aacccgtgtc
     3901 ggctccagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     3961 cggcgagcgc tgccacgtca gacgaagggc gcaggagcgt tcctgatcct tccgcccgga
     4021 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     4081 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     4141 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     4201 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     4261 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagttgcg
     4321 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     4381 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     4441 ttggggggag cgcacaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtaag
     4501 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     4561 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     4621 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcgggtttgt cgtctggttg
     4681 cgggggcggc agttatgcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     4741 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     4801 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     4861 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     4921 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     4981 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     5041 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     5101 cgtttttggc ttttttgtta gacgaagctt gggctgcagg tcgactctag aggatccccg
     5161 ggtaccggta tgacggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     5221 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     5281 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     5341 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     5401 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     5461 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5521 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     5581 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     5641 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     5701 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     5761 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     5821 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     5881 tccggactca gatctcgaga ggaggaggag gagacagaca gcaggatgcc ccacctcgac
     5941 agccccggca gctcccagcc gagacgctcc ttcctctcaa gggtgatcag ggcagcgcta
     6001 ccgttgcagc tgcttctgct gctgctgctg ctcctggcct gcctgctacc tgcctctgaa
     6061 gatgactaca gctgcaccca ggccaacaac tttgcccgat ccttctaccc catgctgcgg
     6121 tacaccaacg ggccacctcc cacctaggaa ttcgatatca agcttatcga taatcaacct
     6181 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6241 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6301 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6361 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6421 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6481 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6541 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6601 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6661 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6721 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     6781 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     6841 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     6901 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6961 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     7021 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     7081 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     7141 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     7201 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     7261 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     7321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7441 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     7501 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7561 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7621 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7681 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     7741 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     7801 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7861 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7921 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7981 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8041 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8101 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8161 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     8221 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8281 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8341 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8401 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8461 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8521 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     8581 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     8641 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     8701 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     8761 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     8821 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     8881 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     8941 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     9001 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     9061 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     9121 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     9181 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9241 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     9301 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9361 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9421 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9481 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9541 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9601 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9661 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9721 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9781 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9841 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9901 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9961 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10021 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10081 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10141 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10201 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10261 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10321 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10381 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10441 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10501 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10561 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10621 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10681 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10741 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10801 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10861 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10921 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10981 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11041 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11101 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11161 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11221 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11281 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11341 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11401 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11461 cccgaaaagt gccacctgac
//
