LOCUS       Exported                8872 bp ds-DNA     circular SYN 01-AUG-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 1, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    194..215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        230..260
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 230 .. 235 / #ffffff / -35
                        2: 236 .. 253 / #ffffff
                        3: 254 .. 260 / #ffffff / -10"
     protein_bind    268..284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     292..308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_binding    complement(474..1174)
                     /label=piggyBacL
                     /note="color: #f7a68a; direction: LEFT"
     terminator      complement(1214..1447)
                     /label=SV40
                     /note="color: #000000; direction: LEFT"
     primer_bind     complement(1320..1334)
                     /label=TCGACCTCGAAACTTGTTTATTGCA
                     /note="color: black; sequence: TCGACCTCGAAACTTGTTTATTGCA"
     CDS             complement(1455..2135)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 1455 .. 2129 / #ff0000
                        2: 2130 .. 2132 / #ff0000 / 1a
                        3: 2133 .. 2135 / #ff0000"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     2142..2165
                     /label=GTCACTTGGTTGTTCACGATCTTG
                     /note="color: black; sequence: GTCACTTGGTTGTTCACGATCTTG"
     promoter        complement(2146..2737)
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
                     /note="color: #ffffff; direction: LEFT"
     enhancer        2741..3223
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
                     /note="color: #a6acb3"
     primer_bind     complement(3196..3223)
                     /label=CGCGTAAAACACAATCAAGTATGAGTCA
                     /note="color: black; sequence: 
                     CGCGTAAAACACAATCAAGTATGAGTCA"
     promoter        3231..3777
                     /label=Opie2 promoter
                     /note="Opie2 promoter"
                     /note="color: #ff8000; direction: RIGHT"
     primer_bind     3237..3257
                     /label=CATACTCGGTGGCCTCCCCAC
                     /note="color: black; sequence: CATACTCGGTGGCCTCCCCAC"
     primer_bind     complement(3759..3777)
                     /label=CACCAGAGACAGGTTGCGG
                     /note="color: black; sequence: CACCAGAGACAGGTTGCGG"
     CDS             join(3778..3780,4029..4625)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="This feature has 2 segments:
                        1: 3778 .. 3780 / #ccffcc
                        2: 4029 .. 4625 / #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     intron          3781..4028
                     /label=Dme-traF
                     /note="dme-TRA"
                     /note="color: #ffffff"
     primer_bind     3781..3810
                     /label=GTACCCACTATATAGTATAACCGATCTGAT
                     /note="color: black; sequence: 
                     GTACCCACTATATAGTATAACCGATCTGAT"
     primer_bind     complement(4004..4028)
                     /label=CTATGTGAAAAGAGTGTGCGGTTAG
                     /note="color: black; sequence: CTATGTGAAAAGAGTGTGCGGTTAG"
     primer_bind     complement(4006..4028)
                     /label=CTATGTGAAAAGAGTGTGCGGTT
                     /note="color: black; sequence: CTATGTGAAAAGAGTGTGCGGTT"
     primer_bind     4028..4047
                     /label=ATGACCGAGTACAAGCCCACGG
                     /note="color: black; sequence: ATGACCGAGTACAAGCCCACGG"
     primer_bind     complement(4609..4626)
                     /label=CTCAGGCACCGGGCTTGC
                     /note="color: black; sequence: CTCAGGCACCGGGCTTGC"
     primer_bind     4633..4657
                     /label=TCGACCTCGAAACTTGTTTATTGCA
                     /note="color: black; sequence: TCGACCTCGAAACTTGTTTATTGCA"
     polyA_signal    4643..4777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(4771..4795)
                     /label=CGGGCCCCCCCTCGACATGATCCAG
                     /note="color: black; sequence: CGGGCCCCCCCTCGACATGATCCAG"
     primer_bind     complement(4781..4795)
                     /label=CGGGCCCCCCCTCGA
                     /note="color: black; sequence: CGGGCCCCCCCTCGA"
     misc_binding    4849..5846
                     /label=piggyBacR
                     /note="color: #f7a68a"
     primer_bind     complement(6564..6580)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     promoter        7054..7158
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7159..8019
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7159 .. 7227 / #ccffcc / signal sequence
                        2: 7228 .. 8019 / #ccffcc
                     Cleavage site after base 7227"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8190..8778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
       61 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      121 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      181 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      241 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      301 gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga gtcgacgctc
      361 gcgcgacttg gtttgccatt ctttagcgcg cgtcgcgtca cacagcttgg ccacaatgtg
      421 gtttttgtca aacgaagatt ctatgacgtg tttaaagttt aggtcgagta aagcgcaaat
      481 cttttttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg
      541 ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc
      601 ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa
      661 cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc
      721 atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat
      781 tttcttgtta tagatatcgt gactaatata taataaaatg ggtagttctt tagacgatga
      841 gcatatcctc tctgctcttc tgcaaagcga tgacgagctt gttggtgagg attctgacag
      901 tgaaatatca gatcacgtaa gtgaagatga cgtccagagc gatacagaag aagcgtttat
      961 agatgaggta catgaagtgc agccaacgtc aagcggtagt gaaatattag acgaacaaaa
     1021 tgttattgaa caaccaggtt cttcattggc ttctaacaga atcttgacct tgccacagag
     1081 gactattaga ggtaagaata aacattgttg gtcaacttca aagtccacga ggcgtagccg
     1141 agtctctgca ctgaacattg tcagatccga gatcggccgg cctaggcgcg ccgtacgcgt
     1201 atcgataagc tttaagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     1261 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     1321 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     1381 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     1441 tagagtcgcg gccgctacag gaacaggtgg tggcggccct cggtgcgctc gtactgctcc
     1501 acgatggtgt agtcctcgtt gtgggaggtg atgtccagct tggagtccac gtagtagtag
     1561 ccgggcagct gcacgggctt cttggccatg tagatggact tgaactccac caggtagtgg
     1621 ccgccgtcct tcagcttcag ggccttgtgg atctcgccct tcagcacgcc gtcgcggggg
     1681 tacaggcgct cggtggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg
     1741 gaggggaagt tcacgccgat gaacttcacc ttgtagatga agcagccgtc ctgcagggag
     1801 gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg
     1861 aagccctcgg ggaaggacag cttcttgtag tcggggatgt cggcggggtg cttcacgtac
     1921 accttggagc cgtactggaa ctggggggac aggatgtccc aggcgaaggg cagggggccg
     1981 cccttggtca ccttcagctt cacggtgttg tggccctcgt aggggcggcc ctcgccctcg
     2041 ccctcgatct cgaactcgtg gccgttcacg gtgccctcca tgcgcacctt gaagcgcatg
     2101 aactccttga tgacgttctt ggaggagcgc accatctcga ggtcacttgg ttgttcacga
     2161 tcttgtcgcc gccagtgtca acttgcaact gaaacaatat ccaacatgaa cgtcaattta
     2221 tactgcccta atggcgaaca cgataacaat atttctttta ttatgccctc taaaaccaac
     2281 gcggttatcg tttatttatt caaattagat atagaacatc cgccgacata caatgttaat
     2341 gcaaaaacgc gtttggtgag cggatacgaa aacagtcggc cgataaacat taatctgagg
     2401 tcgataacac cgtccttgaa cggaacacga ggagcgtacg tgatcagctg cattcgcgcg
     2461 ccgcgccttt atcgagattt atttgcatac aacaagtaca ctgcgccgtt gggatttgtg
     2521 gtaacgcgca cacatgcaga gctgcaagtg tggcacattt tgtctgtgcg caaaaccttt
     2581 gaagccaaaa gtacgaggtc cgttacgggc atgctagcgc acacggacaa tggacccgac
     2641 aaattctacg ccaaggattt aatgataatg tcgggcaacg tatccgttca ttttatcaat
     2701 aacctacaaa aatgtcgcgc gcatcacaaa gacatcgacg cgcgtagaat tctacccgta
     2761 aagcgagttt agttatgagc catgtgcaaa acatgacatc agcttttatt tttataacaa
     2821 atgacatcat ttcttgattg tgttttacac gtagaattct actcgtaaag cgagttcagt
     2881 tttgaaaaac aaatgacatc atctttttga ttgtgcttta caagtagaat tctacccgta
     2941 aatcaagttc ggttttgaaa aacaaatgag tcatattgta tgatatcata ttgcaaaaca
     3001 aatgactcat caatcgatcg tgcgttacac gtagaattct actcgtaaag cgagtttatg
     3061 agccgtgtgc aaaacatgac atcatctcga tttgaaaaac aaatgacatc atccactgat
     3121 cgtgcgttac aagtagaatt ctactcgtaa agccagttcg gttatgagcc gtgtgcaaaa
     3181 catgacatca gcttatgact catacttgat tgtgttttac gcggtttaaa cgcgcccata
     3241 ctcggtggcc tccccaccac caactttttt gcactgcaaa aaaacacgct tttgcacgcg
     3301 ggcccataca tagtacaaac tctacgtttc gtagactatt ttacataaat agtctacacc
     3361 gttgtatacg ctccaaatac actaccacac attgaacctt tttgcagtgc aaaaaagtac
     3421 gtgtcggcag tcacgtaggc cggccttatc gggtcgcgtc ctgtcacgta cgaatcacat
     3481 tatcggaccg gacgagtgtt gtcttatcgt gacaggacgc cagcttcctg tgttgctaac
     3541 cgcagccgga cgcaactcct tatcggaaca ggacgcgcct ccatatcagc cgcgcgttat
     3601 ctcatgcgcg tgaccggaca cgaggcgccc gtcccgctta tcgcgcctat aaatacagcc
     3661 cgcaacgatc tggtaaacac agttgaacag catctgttac agcgacacaa catgagccgg
     3721 tccaacaacg ccaacgcgcc cacgccatcc aaccgccgcc gcaacctgtc tctggtgatg
     3781 gtacccacta tatagtataa ccgatctgat cagattctga tccagtgcca tctttttgtt
     3841 gttttttttc tagtgtcata ttgtgtgaaa tgtgaaatgg atgagaatga acgctggacg
     3901 accagacaga gaaagagaag ctaggacaat aggactctca actgcgcatt acgtggattc
     3961 cgtctccgac gatgcgccaa acactatgcg ttagatgcat tgactaaccg cacactcttt
     4021 tcacatagac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     4081 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     4141 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4201 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4261 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     4321 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     4381 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     4441 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     4501 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     4561 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4621 cctgagagat cttcgacctc gaaacttgtt tattgcagct tataatggtt acaaataaag
     4681 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4741 gtccaaactc atcaatgtat cttatcatgt ctggatcatg tcgagggggg gcccgattta
     4801 aataaacccc gggcgagctc gaattaacca ttgtgggaac cgtgcgatca aacaaacgcg
     4861 agataccgga agtactgaaa aacagtcgct ccaggccagt gggaacatcg atgttttgtt
     4921 ttgacggacc ccttactctc gtctcatata aaccgaagcc agctaagatg gtatacttat
     4981 tatcatcttg tgatgaggat gcttctatca acgaaagtac cggtaaaccg caaatggtta
     5041 tgtattataa tcaaactaaa ggcggagtgg acacgctaga ccaaatgtgt tctgtgatga
     5101 cctgcagtag gaagacgaat aggtggccta tggcattatt gtacggaatg ataaacattg
     5161 cctgcataaa ttcttttatt atatacagcc ataatgtcag tagcaaggga gaaaaggttc
     5221 aaagtcgcaa aaaatttatg agaaaccttt acatgagcct gacgtcatcg tttatgcgta
     5281 agcgtttaga agctcctact ttgaagagat atttgcgcga taatatctct aatattttgc
     5341 caaatgaagt gcctggtaca tcagatgaca gtactgaaga gccagtaatg aaaaaacgta
     5401 cttactgtac ttactgcccc tctaaaataa ggcgaaaggc aaatgcatcg tgcaaaaaat
     5461 gcaaaaaagt tatttgtcga gagcataata ttgatatgtg ccaaagttgt ttctgactga
     5521 ctaataagta taatttgttt ctattatgta taagttaagc taattactta ttttataata
     5581 caacatgact gtttttaaag tacaaaataa gtttattttt gtaaaagaga gaatgtttaa
     5641 aagttttgtt actttataga agaaattttg agtttttgtt tttttttaat aaataaataa
     5701 acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact
     5761 taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc
     5821 aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaaa
     5881 taatagtttc taattttttt attattcagc ctgctgtcgt gaataccgta tatctcaacg
     5941 ctgtctgtga gattgtcgta ttctagcctt tttagttttt cgctcatcga cttgatattg
     6001 tccgacacat tttcgtcgat ttgcgttttg atcaaagact tgagcagaga cacgttaatc
     6061 aactgttcaa attgatccat attaacgata tcaacccgat gcgtatatgg tgcgtaaaat
     6121 atatttttta accctcttat actttgcact ctgcgttaat acgcgttcgt gtacagacgt
     6181 aatcatgttt tcttttttgg ataaaactcc tactgagttt gacctcatat tagaccctca
     6241 caagttgcaa aacgtggcat tttttaccaa tgaagaattt aaagttattt taaaaaattt
     6301 catcacagat ttaaagaaga accaaaaatt aaattattta atcgaccagt taatcaacgt
     6361 gtacacagag cgcaaaaaac acgcagcccg acgtgttggc taaaattatt aaatcaactt
     6421 gtgttatagt cacgatttgc cgtccaacgt gttcctcaaa aagttgaaga ccaacaagtt
     6481 tacggacact agttaattat ttgattttgc cccacttcat tttgtgggat cacaattttg
     6541 ttatatttta aacaaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6601 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6661 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6721 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     6781 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     6841 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6901 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     6961 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     7021 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7081 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7141 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7201 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7261 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7321 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7381 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7441 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7501 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7561 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7621 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7681 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7741 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7801 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7861 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7921 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7981 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8041 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8101 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     8341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8401 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8461 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8521 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8581 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8641 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8701 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8761 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8821 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga ta
//
