LOCUS       pOTTC1706_-_pAAV        6779 bp DNA     circular SYN 13-MAY-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
COMMENT     pOTTC1706 was constructed by Chris Richie at GEVVC/NIDA (CR2083_C).
            CR2083_C and CR2083_D are both OK
            
            New DNA from 1 to 6326
            pOTTC1553 - pAAV EF1a NucEnv-EGFP.ape - Copy from 1 to 6326
            pOTTC1553 was constructed by Chris Richie at GEVVC/NIDA (CR1852_D).
            
            
            bakcbone was pOTTC407 - pAAV EF1a eGFP was created by Mari 
            Savolainen on 09/28/2012 (MS08).  Addgene 60058
            Described in Savolainen et al. (2014).  The beneficial effect of a 
            prolyl oligopeptidase inhibitor, KYP-2047, on alpha-synuclein 
            clearance and autophagy in A30P transgenic mouse.  Neurobiology of 
            Disease.  DOI: 10.1016/j.nbd.2014.04.003
FEATURES             Location/Qualifiers
     source          1..6779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..141
                     /label=ITR (AAV)
                     /note="color: #804000"
     promoter        167..481
                     /label=mU6 promoter - mouse
                     /note="color: #804000"
     protein_bind    483..502
                     /label=CR2037 mGrid1 390 F
                     /note="color: #ff8080"
     misc_RNA        507..578
                     /label=Constant region for chimeric gRNA
                     /note="color: #800080; direction: RIGHT"
     promoter        609..1872
                     /label=EF-1a promoter
                     /note="color: #408080"
     CDS             1898..2881
                     /label=EGFP-KASH
                     /label=NucEnv-EGFP
                     /note="color: #0080c0; direction: RIGHT"
     CDS             2633..2881
                     /label=KASH domain of Nesprin2
                     /note="/dnas_title=KASH domain of Nesprin2"
                     /note="/vntifkey=4"
                     /note="color: #0080c0; direction: RIGHT"
     misc_RNA        2896..3504
                     /label=WPRE
                     /note="color: #800080; direction: RIGHT"
     polyA_signal    3525..4005
                     /label=hGH polyA
                     /note="color: #8080ff"
     LTR             complement(4042..4182)
                     /label=ITR (AAV)
                     /label=ITR (AAV)(1)
                     /note="color: #ff8000; direction: LEFT"
     rep_origin      4257..4712
                     /label=M13 origin
                     /note="color: #808080"
     rep_origin      4274..4580
                     /label=f1 origin
                     /note="color: #808080"
     promoter        5000..5098
                     /label=Bla promoter (prokaryotic)
                     /note="color: #804000"
     CDS             5099..5956
                     /label=AmpR
                     /note="color: #0080c0; direction: RIGHT"
     rep_origin      6054..6736
                     /label=ColE1 origin
                     /note="color: #808080"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgtct cagatagatc cgacgccgcc
      181 atctctaggc ccgcgccggc cccctcgcac agacttgtgg gagaagctcg gctactcccc
      241 tgccccggtt aatttgcata taatatttcc tagtaactat agaggcttaa tgtgcgataa
      301 aagacagata atctgttctt tttaatacta gctacatttt acatgatagg cttggatttc
      361 tataagagat acaaatacta aattattatt ttaaaaaaca gcacaaaagg aaactcaccc
      421 taactgtaaa gtaattgtgt gttttgagac tataaatatc ccttggagaa aagccttgtt
      481 tggaacccta gccctgacgg cggttttaga gctagaaata gcaagttaaa ataaggctag
      541 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttgtact gagacgccgc
      601 ggtcgcgtaa gctttgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
      661 atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
      721 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
      781 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
      841 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
      901 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
      961 ctttacgggt tatggccctt gcgtgccttg aattacttcc actggctgca gtacgtgatt
     1021 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     1081 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     1141 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     1201 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     1261 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     1321 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     1381 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     1441 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     1501 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     1561 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     1621 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     1681 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     1741 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     1801 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     1861 caggtgtcgt gaggtaccgg atccaccggt cgccaccatg gtgagcaagg gcgaggagct
     1921 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1981 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     2041 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     2101 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2161 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2221 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2281 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2341 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2401 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2461 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     2521 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2581 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc gagaggagga
     2641 ggaggagaca gacagcagga tgccccacct cgacagcccc ggcagctccc agccgagacg
     2701 ctccttcctc tcaagggtga tcagggcagc gctaccgttg cagctgcttc tgctgctgct
     2761 gctgctcctg gcctgcctgc tacctgcctc tgaagatgac tacagctgca cccaggccaa
     2821 caactttgcc cgatccttct accccatgct gcggtacacc aacgggccac ctcccaccta
     2881 ggaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     2941 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3001 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3061 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3121 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3181 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3241 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3301 atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc
     3361 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3421 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3481 ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg gcatccctgt
     3541 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     3601 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     3661 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     3721 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     3781 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     3841 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     3901 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     3961 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc
     4021 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     4081 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4141 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     4201 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     4261 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4321 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4381 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4441 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     4501 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4561 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg
     4621 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4681 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     4741 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     4801 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     4861 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     4921 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4981 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5041 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5101 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5161 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5221 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5281 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5341 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5401 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5461 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5521 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5581 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5641 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5701 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5761 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5821 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5881 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5941 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6001 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6061 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6241 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
