LOCUS       Exported                3325 bp ds-DNA     circular SYN 08-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    JI401_E1A_Hexon_qRTPCR_STD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 8, 2019 from SnapGene Viewer 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="synthetic DNA construct"
                     /note="dnas_title=JI401_E1A_Hexon_qRTPCR_STD"
     misc_feature    600..616
                     /label=PCR_primer
                     /note="M13F seq_primer"
                     /note="M13 (-20) forward"
     misc_feature    690..889
                     /note="851-1050 of pJM17"
     misc_feature    complement(730..849)
                     /label=PCR_product
                     /note="E1A PCR_product"
                     /note="E1A PCR amplicon
                     TB964/TB1038"
     misc_feature    730..753
                     /label=PCR_primer
                     /note="TB964 PCR_primer"
                     /note="TB964"
     misc_feature    complement(829..849)
                     /label=PCR_primer
                     /note="TB1038 PCR_primer"
                     /note="TB1038"
     misc_feature    890..1089
                     /note="25787-25986 of pJM17"
     misc_feature    complement(919..1060)
                     /label=PCR_product
                     /note="Hexon PCR_product"
                     /note="Hexon PCR amplicon
                     TB1045/TB1046"
     misc_feature    919..942
                     /label=PCR_primer
                     /note="TB1045 PCR_primer"
                     /note="TB1045"
     misc_feature    complement(1037..1060)
                     /label=PCR_primer
                     /note="TB1046 PCR_primer"
                     /note="TB1046"
     misc_feature    1090..689
                     /note="phagemid pBluescriptII SK(+)"
     misc_feature    complement(1172..1187)
                     /label=PCR_primer
                     /note="M13R seq_primer"
                     /note="M13 reverse"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcccgagcag ccggagcaga gagccttggg
      721 tccggtttct atgccaaacc ttgtaccgga ggtgatcgat cttacctgcc acgaggctgg
      781 ctttccaccc agtgacgacg aggatgaaga gggtgaggag tttgtgttag attatgtgga
      841 gcaccccggg cacggttgca ggtcttgtca ttatcaccgg aggaatacgc agaaaaagtt
      901 tctttgcgat cgcacccttt ggcgcatccc attctccagt aactttatgt ccatgggcgc
      961 actcacagac ctgggccaaa accttctcta cgccaactcc gcccacgcgc tagacatgac
     1021 ttttgaggtg gatcccatgg acgagcccac ccttctttat gttttgtttg aagtctttga
     1081 cgtggtccgc tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
     1141 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1201 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1261 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1321 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1381 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1441 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1501 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1561 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1621 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1681 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1741 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1801 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1861 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1921 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1981 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2041 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2101 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2161 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2221 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2281 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2341 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2401 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2461 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2521 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2581 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2641 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2701 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2761 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2821 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2881 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2941 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3001 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3061 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3121 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3181 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3241 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3301 cgcacatttc cccgaaaagt gccac
//
