LOCUS       Exported                9741 bp ds-DNA    circular SYN 06-AUG-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pNS3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Avalos
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2019 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..122
                     /note="lac Promoter"
     protein_bind    13..34
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    87..103
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     111..127
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        148..167
                     /note="T3_P"
     misc_feature    207..373
                     /note="YARCTy1-1"
                     /note=">YARCTy1-1 YARCTy1-1 SGDID:S000006792, 
                     chrI:160238..166162
                     http://www.yeastgenome.org/locus/S000006792/overview
                     
                     Ty1 element, LTR retrotransposon of the Copia 
                     (Pseudoviridae) group; contains genes TYA Gag and TYB Pol, 
                     encoding proteins involved in structure and function of 
                     virus-like particles, flanked by two direct repeats; 
                     mutated in S288C   "
     misc_feature    207..373
                     /note="YARCdelta5_5' repeat"
                     /note=">YARCdelta5 YARCdelta5 SGDID:S000006790, 
                     chrI:165826..166162
                     http://www.yeastgenome.org/locus/S000006790/sequence"
     misc_feature    207..373
                     /note="∂_5'Half"
     protein_bind    374..407
                     /bound_moiety="Cre recombinase"
                     /note="lox71"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     terminator      complement(408..427)
                     /note="from TEF"
     gene            complement(459..1173)
                     /note="bleMX6"
                     /note="yeast selectable marker conferring phleomycin 
                     resistance"
     terminator      complement(459..656)
                     /note="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(662..1036)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1037..1380)
                     /note="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     promoter        complement(1174..1434)
                     /note="from TEF"
     protein_bind    1435..1468
                     /bound_moiety="Cre recombinase"
                     /note="lox66"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1469..1485
                     /note="minimal sgi"
     misc_feature    complement(1469..1483)
                     /note="SGI-S1"
     misc_feature    complement(1469..1483)
                     /note="MCS"
                     /note="poly-linker region"
     misc_feature    1484..1485
                     /note="SGI-S2"
     misc_feature    complement(1486..5919)
                     /note="MCS"
                     /note="poly-linker region"
     misc_feature    complement(1486..1490)
                     /note="SGI-S1"
     CDS             1491..2957
                     /note="4"
                     /note="lacZ' beta-galactosidase alpha peptide"
     misc_feature    complement(1491..2957)
                     /note="21"
                     /note="poly-linker region"
     misc_feature    1500..2957
                     /note="ADH1Long Promoter"
     misc_feature    complement(2968..5856)
                     /note="MCS"
                     /note="poly-linker region"
     CDS             2968..3609
                     /codon_start=1
                     /note="FUS_N"
                     /translation="MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQST
                     DTSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQP
                     APSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNS
                     SSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRG"
     misc_feature    3619..4326
                     /note="Fusion Red"
     CDS             3619..3624
                     /codon_start=1
                     /note="mCherry"
                     /translation="MV"
     misc_feature    complement(4327..5817)
                     /note="MCS"
                     /note="poly-linker region"
     misc_feature    4327..5817
                     /note="Cry2olig(PHR)"
     misc_feature    5857..5926
                     /note="MCS"
     misc_feature    5857..5859
                     /note="KOZAK"
     misc_feature    5926..5931
                     /note="STOP"
     misc_feature    5935..6227
                     /note="ACT1 t"
     misc_feature    6232..6274
                     /note="SGI-S2"
     misc_feature    6252..6275
                     /note="minimal sgi"
     misc_feature    6276..6445
                     /note="YARCdelta5_5' repeat"
                     /note=">YARCdelta5 YARCdelta5 SGDID:S000006790, 
                     chrI:165826..166162
                     http://www.yeastgenome.org/locus/S000006790/sequence"
     misc_feature    6276..6445
                     /note="∂_3'Half"
     promoter        complement(6468..6486)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6493..6509)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(6513..6536)
                     /note="M13F(-47)"
     rep_origin      6650..7105
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7742..7846
                     /gene="bla"
                     /note="AmpR promoter"
     misc_feature    7847..8705
                     /note="AmpR"
ORIGIN
        1 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
       61 gcttccggct cctatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      121 ctatgaccat gattacgcca agctcggaat taaccctcac taaagggaac aaaagctggg
      181 ccggccattt aaatgtttaa acaacctgtt ggaatagaaa tcaactatca tctactaact
      241 agtatttaca ttactagtat attatcatat acggtgttag aagatgacgc aaatgatgag
      301 aaatagtcat ctaaattagt ggaagctgaa acgcaaggat tgataatgta ataggatcaa
      361 tgaatataaa cattaccgtt cgtatagcat acattatacg aagttatatt aagggttctc
      421 gagagctcgg gatggcggcg ttagtatcga atcgacagca gtatagcgac cagcattcac
      481 atacgattga cgcatgatat tactttctgc gcacttaact tcgcatctgg gcagatgatg
      541 tcgaggcgaa aaaaaatata aatcacgcta acatttgatt aaaatagaac aactacaata
      601 taaaaaaact atacaaatga caagttcttg aaaacaagaa tctttttatt gtcagtactg
      661 atcagtcctg ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga
      721 actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga
      781 agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca
      841 cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg aacagggtca
      901 cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc
      961 ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcgc
     1021 tggtcaactt ggccatggtt gtttatgttc ggatgtgatg tgagaactgt atcctagcaa
     1081 gattttaaaa ggaagtatat gaaagaagaa cctcagtggc aaatcctaac cttttatatt
     1141 tctctacagg ggcgcggcgt ggggacaatt caacgcgtct gtgaggggag cgtttccctg
     1201 ctcgcaggtc tgcagcgagg agccgtaatt tttgcttcgc gccgtgcggc catcaaaatg
     1261 tatggatgca aatgattata catggggatg tatgggctaa atgtacgggc gacagtcaca
     1321 tcatgcccct gagctgcgca cgtcaagact gtcaaggagg gtattctggg cctccatgtc
     1381 gctggccggg tgacccggcg gggacgaggc aagctaaaca gatctctaga cctaataact
     1441 tcgtatagca tacattatac gaacggtacc cgggaaacag ctgcgccggg aaacagctgg
     1501 ccgggatcga agaaatgatg gtaaatgaaa taggaaatca aggagcatga aggcaaaaga
     1561 caaatataag ggtcgaacga aaaataaagt gaaaagtgtt gatatgatgt atttggcttt
     1621 gcggcgccga aaaaacgagt ttacgcaatt gcacaatcat gctgactctg tggcggaccc
     1681 gcgctcttgc cggcccggcg ataacgctgg gcgtgaggct gtgcccggcg gagttttttg
     1741 cgcctgcatt ttccaaggtt taccctgcgc taaggggcga gattggagaa gcaataagaa
     1801 tgccggttgg ggttgcgatg atgacgacca cgacaactgg tgtcattatt taagttgccg
     1861 aaagaacctg agtgcatttg caacatgagt atactagaag aatgagccaa gacttgcgag
     1921 acgcgagttt gccggtggtg cgaacaatag agcgaccatg accttgaagg tgagacgcgc
     1981 ataaccgcta gagtactttg aagaggaaac agcaataggg ttgctaccag tataaataga
     2041 caggtacata caacactgga aatggttgtc tgtttgagta cgctttcaat tcatttgggt
     2101 gtgcacttta ttatgttaca atatggaagg gaactttaca cttctcctat gcacatatat
     2161 taattaaagt ccaatgctag tagagaaggg gggtaacacc cctccgcgct cttttccgat
     2221 ttttttctaa accgtggaat atttcggata tccttttgtt gtttccgggt gtacaatatg
     2281 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
     2341 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
     2401 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
     2461 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
     2521 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
     2581 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
     2641 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
     2701 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
     2761 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
     2821 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
     2881 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
     2941 gcatacaatc aactccattg cggccgcatg gcctcaaacg attataccca acaagcaacc
     3001 caaagctatg gggcctaccc cacccagccc gggcagggct attcccagca gagcagtcag
     3061 ccctacggac agcagagtta cagtggttat agccagtcca cggacacttc aggctatggc
     3121 cagagcagct attcttctta tggccagagc cagaacacag gctatggaac tcagtcaact
     3181 ccccagggat atggctcgac tggcggctat ggcagtagcc agagctccca atcgtcttac
     3241 gggcagcagt cctcctatcc tggctatggc cagcagccag ctcccagcag cacctcggga
     3301 agttacggta gcagttctca gagcagcagc tatgggcagc cccagagtgg gagctacagc
     3361 cagcagccta gctatggtgg acagcagcaa agctatggac agcagcaaag ctataatccc
     3421 cctcagggct atggacagca gaaccagtac aacagcagca gtggtggtgg aggtggaggt
     3481 ggaggtggag gtaactatgg ccaagatcaa tcctccatga gtagtggtgg tggcagtggt
     3541 ggcggttatg gcaatcaaga ccagagtggt ggaggtggca gcggtggcta tggacagcag
     3601 gaccgtggag gtggaggaat ggtgagcgag ctgattaagg agaacatgcc catgaagctg
     3661 tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag
     3721 ccctacgagg gcacccagac catgagaatc aaggtcgtcg agggcggccc tctccccttc
     3781 gccttcgaca tcctggctac cagcttcatg tacggcagca gaaccttcat caagcaccct
     3841 ccgggcatcc ccgacttctt taagcagtcc ttccctgagg gcttcacatg ggagagagtc
     3901 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     3961 tgcctcatct acaacgtcaa ggttagaggg gtgaacttcc cagccaacgg ccctgtgatg
     4021 cagaagaaaa cactcggctg ggaggcctcc accgagacga tgtaccccgc tgacggcggc
     4081 ctggaaggcg catgtgacat ggccctgaag ctcgtgggcg ggggccacct gatctgcaac
     4141 cttgagacca catacagatc caagaaaccc gctacgaacc tcaagatgcc cggcgtctac
     4201 aacgtggacc acagactgga aagaatcaag gaggccgacg atgagaccta cgtcgagcag
     4261 cacgaggtgg ctgtggccag atactctact ggtggcgctg gtgatggagg taaagcggcc
     4321 acgcgtaaga tggacaaaaa gactatagtt tggtttagaa gagacctaag gattgaggat
     4381 aatcctgcat tagcagcagc tgctcacgaa ggatctgttt ttcctgtctt catttggtgt
     4441 cctgaagaag aaggacagtt ttatcctgga agagcttcaa gatggtggat gaaacaatca
     4501 cttgctcact tatctcaatc cttgaaggct cttggatctg acctcacttt aatcaaaacc
     4561 cacaacacga tttcagcgat cttggattgt atccgcgtta ccggtgctac aaaagtcgtc
     4621 tttaaccacc tctatgatcc tgtttcgtta gttcgggacc ataccgtaaa ggagaagctg
     4681 gtggaacgtg ggatctctgt gcaaagctac aatggagatc tattgtatga accgtgggag
     4741 atatactgcg aaaagggcaa accttttacg agtttcaatt cttactggaa gaaatgctta
     4801 gatatgtcga ttgaatccgt tatgcttcct cctccttggc ggttgatgcc aataactgca
     4861 gcggctgaag cgatttgggc gtgttcgatt gaagaactag ggctggagaa tgaggccgag
     4921 aaaccgagca atgcgttgtt aactagagct tggtctccag gatggagcaa tgctgataag
     4981 ttactaaatg agttcatcga gaagcagttg atagattatg caaagaacag caagaaagtt
     5041 gttgggaatt ctacttcact actttctccg tatctccatt tcggggaaat aagcgtcaga
     5101 cacgttttcc agtgtgcccg gatgaaacaa attatatggg caagagataa gaacagtgaa
     5161 ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt taagagagta ttctcggtat
     5221 atatgtttca acttcccgtt tactcacgag caatcgttgt tgagtcatct tcggtttttc
     5281 ccttgggatg ctgatgttga taagttcaag gcctggagac aaggcaggac cggttatccg
     5341 ttggtggatg ccggaatgag agagctttgg gctaccggat ggatgcataa cagaataaga
     5401 gtgattgttt caagctttgc tgtgaagttt cttctccttc catggaaatg gggaatgaag
     5461 tatttctggg atacactttt ggatgctgat ttggaatgtg acatccttgg ctggcagtat
     5521 atctctggga gtatccccga tggccacgag cttgatcgct tggacaatcc cgcgttacaa
     5581 ggcgccaaat atgacccaga aggtgagtac ataaggcaat ggcttcccga gcttgcgaga
     5641 ttgccaactg aatggatcca tcatccatgg gacgctcctt taaccgtact caaagcttct
     5701 ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag acatcgacac agctcgtgag
     5761 ctactagcta aagctatttc aagaacccgt ggagcacaga tcatgatcgg agcagcaggt
     5821 tcagggagcg gatccggctc agggagtgcg gccgcagcta gctctagaac tagtggatcc
     5881 ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagtaatg atcatctctg
     5941 cttttgtgcg cgtatgttta tgtatgtacc tctctctcta tttctatttt taaaccaccc
     6001 tctcaataaa ataaaaataa taaagtattt ttaaggaaaa gacgtgttta agcactgact
     6061 ttatctactt tttgtacgtt ttcattgata taatgtgttt tgtctctccc ttttctacga
     6121 aaatttcaaa aattgaccaa aaaaaggaat atatatacga aaaactatta tatttatata
     6181 tcatagtgtt gataaaaaat gtttatccat tggaccgtgt atcatattta tcgccggcga
     6241 ataaattaag gcgcgccaac atatccggag gtaccataaa acggaatgag gaataatcgt
     6301 aatattagta tgtagaaata tagattccat tttgaggatt cctatatcct cgaggagaac
     6361 ttctagtata ttctgtatac ctaatattat agcctttatc aacaatggaa tcccaacaat
     6421 tatctcaaca ttcacccatt tctcagttta aacggccacg ctggccccct atagtgagtc
     6481 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     6541 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     6601 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
     6661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6781 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6841 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6901 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6961 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7021 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7081 aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     7141 gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct aatttgtgag
     7201 tttagtatta ggatctctac ccttggcgaa aagtcctctg ccaacaatga tgatatctga
     7261 tccaccactt acaacttcgt cgagggttct gtactgctga cccaatatgc gtatatatac
     7321 caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa
     7381 aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaaaag
     7441 gtggtatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     7501 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     7561 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     7621 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     7681 ataatggttt cttagacgtc acgcgccgag gacgtcaggt ggcacttttc ggggaaatgt
     7741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9661 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9721 cgactggaaa gcgggcagtg a
//
