LOCUS       pBW005                  3917 bp ds-DNA     circular     27-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     Promoter        2661..2695
                     /label="J23106"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1791..2462
                     /label="RFP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     Promoter region 1395..1400
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        1353..1426
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1458..2465
                     /label="Fd L10 RFP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1755..1790
                     /label="L12"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2684..2689
                     /label="-10"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(join(3594..3917,1..359))
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     misc_feature    1339..1344
                     /label="mutated BbsI site"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2696..2696
                     /label="+1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             2730..3356
                     /label="tetR"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    2463..2466
                     /label="4bp overhang"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2487..2615
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     Promoter region 1372..1377
                     /label="-35"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(486..1145)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     terminator      364..469
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     RBS             2696..2729
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     Promoter region 1407..1407
                     /label="+1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Terminator      3357..3461
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     RBS             1427..1456
                     /label="sRBS (2466.11 AU)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1773..1776
                     /label="4bp overhang"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Promoter region 1378..1396
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2661..2666
                     /label="-35"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Promoter region 1353..1371
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
       61 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
      121 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
      181 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
      241 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
      301 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAC
      361 TAGTGCTTGG ATTCTCACCA ATAAAAAACG CCCGGCGGCA ACCGAGCGTT CTGAACAAAT
      421 CCAGATGGAG TTCTGAGGTC ATTACTGGAT CTATCAACAG GAGTCCAAGC GAGCTCGATA
      481 TCAAATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG
      541 CCGACATGGA AGCCATCACA GACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC
      601 TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA
      661 TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC
      721 ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT
      781 TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA
      841 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC
      901 AGCTCACCGT CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA
      961 ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC
     1021 GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA
     1081 AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC
     1141 TCCATTTTAG CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT
     1201 GATCTTATTT CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTTT
     1261 CGCCAGATAT CGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC
     1321 GTATCACGAG GCCCTTTCGT CTTGACCTCG AGTCCCTATC AGTGATAGAG ATTGACATCC
     1381 CTATCAGTGA TAGAGATACT GAGCACATCA GCAGGACGCA CTGACCTAAA AATCCGATCC
     1441 CTCTTTAGTT CTCAATCATG GCAACCTATA AAGTTACACT TATTAACGAA GCTGAAGGTC
     1501 TAAACAAAAC AATTGAAGTT CCAGATGATC AGTACATTTT AGATGCGGCT GAAGAAGCTG
     1561 GTATAGACTT ACCGTATTCT TGTCGTGCTG GTGCTTGCTC TACCTGTGCT GGTAAACTCA
     1621 TATCCGGTAC TGTCGATCAA AGCGATCAGT CATTTTTAGA CGACGATCAA ATTGAAGCTG
     1681 GATACGTTCT CACCTGCGTT GCTTACCCAA CTTCCGATTG CGTCATCGAA ACACACAAAG
     1741 AAGAAGAGCT TTACGGGGGC TCTGGAGGTT CTGGAGGTTC TGGTGGGTCC GCGAGTAGCG
     1801 AAGACGTTAT CAAAGAGTTC ATGCGTTTCA AAGTTCGTAT GGAAGGTTCC GTTAACGGTC
     1861 ACGAGTTCGA AATCGAAGGT GAAGGTGAAG GTCGTCCGTA CGAAGGTACC CAGACCGCTA
     1921 AACTGAAAGT TACCAAAGGT GGTCCGCTGC CGTTCGCTTG GGACATCCTG TCCCCGCAGT
     1981 TCCAGTACGG TTCCAAAGCT TACGTTAAAC ACCCGGCTGA CATCCCGGAC TACCTGAAAC
     2041 TGTCCTTCCC GGAAGGTTTC AAATGGGAAC GTGTTATGAA CTTCGAAGAC GGTGGTGTTG
     2101 TTACCGTTAC CCAGGACTCC TCCCTGCAAG ACGGTGAGTT CATCTACAAA GTTAAACTGC
     2161 GTGGTACCAA CTTCCCGTCC GACGGTCCGG TTATGCAGAA AAAAACCATG GGTTGGGAAG
     2221 CTTCCACCGA ACGTATGTAC CCGGAAGACG GTGCTCTGAA AGGTGAAATC AAAATGCGTC
     2281 TGAAACTGAA AGACGGTGGT CACTACGACG CTGAAGTTAA AACCACCTAC ATGGCTAAAA
     2341 AACCGGTTCA GCTGCCGGGT GCTTACAAAA CCGACATCAA ACTGGACATC ACCTCCCACA
     2401 ACGAAGACTA CACCATCGTT GAACAGTACG AACGTGCTGA AGGTCGTCAC TCCACCGGTG
     2461 CTTGAgaatt cTGATAATAA TCTAGACCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA
     2521 AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCTACT AGAGTCACAC
     2581 TGGCTCACCT TCGGGTGGGC CTTTCTGCGT TTATAGGTAC CCGTGGATCC TCTAGAGTCG
     2641 ACCTGCAGGC ATGCAAGCTT TTTACGGCTA GCTCAGTCCT AGGTATAGTG CTAGCCCAGC
     2701 CAGAGAAACA CTCTTTAACA GGGGGTAGTA TGATGTCTCG TTTAGATAAA AGTAAAGTGA
     2761 TTAACAGCGC ATTAGAGCTG CTTAATGAGG TCGGAATCGA AGGTTTAACA ACCCGTAAAC
     2821 TCGCCCAGAA GCTAGGTGTA GAGCAGCCTA CATTGTATTG GCATGTAAAA AATAAGCGGG
     2881 CTTTGCTCGA CGCCTTAGCC ATTGAGATGT TAGATAGGCA CCATACTCAC TTTTGCCCTT
     2941 TAGAAGGGGA AAGCTGGCAA GATTTTTTAC GTAATAACGC TAAAAGTTTT AGATGTGCTT
     3001 TACTAAGTCA TCGCGATGGA GCAAAAGTAC ATTTAGGTAC ACGGCCTACA GAAAAACAGT
     3061 ATGAAACTCT CGAAAATCAA TTAGCCTTTT TATGCCAACA AGGTTTTTCA CTAGAGAATG
     3121 CATTATATGC ACTCAGCGCA GTGGGGCATT TTACTTTAGG TTGCGTATTG GAAGATCAAG
     3181 AGCATCAAGT CGCTAAAGAA GAAAGGGAAA CACCTACTAC TGATAGTATG CCGCCATTAT
     3241 TACGACAAGC TATCGAATTA TTTGATCACC AAGGTGCAGA GCCAGCCTTC TTATTCGGCC
     3301 TTGAATTGAT CATATGCGGA TTAGAAAAAC AACTTAAATG TGAAAGTGGG TCTTAAGGCA
     3361 TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC
     3421 GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGCCCTAG ACCTAGGCGT TCGGCTGCGG
     3481 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC
     3541 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
     3601 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
     3661 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
     3721 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
     3781 CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
     3841 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
     3901 TTATCCGGTA ACTATCG
//