LOCUS       pBW008                  4046 bp ds-DNA     circular     27-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1549..1677
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    486..1317
                     /label="Trans-ER LBD"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             360..389
                     /label="sRBS (2466.11 AU)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             1758..1791
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     misc_feature    1723..1728
                     /label="-35"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    391..1527
                     /label="Fd ER 30"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1758..1758
                     /label="+1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Terminator      2419..2523
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     terminator      3343..3448
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    481..485
                     /label="5 bp duplication"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    486..487
                     /label="R1-L"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Promoter region 305..310
                     /label="-35"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(join(3465..4046,1..78))
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     Promoter        1723..1757
                     /label="J23106"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(1316..1317)
                     /label="R1-R"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        286..359
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             1792..2418
                     /label="tetR"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     Promoter region 311..329
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Promoter region 340..340
                     /label="+1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Promoter region 328..333
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1746..1751
                     /label="-10"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Promoter region 286..304
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    272..277
                     /label="mutated BbsI site"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(2656..3338)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
ORIGIN
        1 CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC
       61 AGTGATTTTT TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA
      121 TACGCCCGGT AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC
      181 AACGTCTCAT TTTCGCCAGA TATCGACGTC TAAGAAACCA TTATTATCAT GACATTAACC
      241 TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTGACC TCGAGTCCCT ATCAGTGATA
      301 GAGATTGACA TCCCTATCAG TGATAGAGAT ACTGAGCACA TCAGCAGGAC GCACTGACCT
      361 AAAAATCCGA TCCCTCTTTA GTTCTCAATC ATGGCAACCT ATAAAGTTAC ACTTATTAAC
      421 GAAGCTGAAG GTCTAAACAA AACAATTGAA GTTCCAGATG ATCAGTACAT TTTAGATGCG
      481 GCTGATGCTG CAGCTGGGGG CGGAGGTTCT GGAGGTGGTG GGTCCAAGAA GAATTCACTG
      541 GCGTTGTCGC TTACGGCTGA CCAAATGGTT TCTGCTCTGC TTGACGCCGA ACCTCCGATC
      601 CTGTACTCTG AATACGACCC GACTCGTCCT TTTTCAGAGG CGAGTATGAT GGGGCTGCTT
      661 ACGAATCTGG CAGACCGCGA GTTGGTACAT ATGATTAATT GGGCTAAACG TGTGCCGGGT
      721 TTCGTGGACT TGACCCTGCA TGACCAGGTC CACCTGTTAG AATGCGCCTG GTTAGAGATT
      781 TTAATGATCG GACTTGTCTG GCGTAGCATG GAACACCCCG GAAAACTGTT ATTTGCCCCT
      841 AACTTGCTGC TGGACCGCAA CCAGGGGAAG TGTGTCGAAG GAATGGTGGA AATTTTCGAC
      901 ATGCTTCTTG CCACTTCCTC GCGTTTTCGT ATGATGAATT TACAGGGAGA AGAGTTCGTT
      961 TGTTTAAAGT CAATCATCTT ACTGAATAGC GGGGTTTACA CATTCTTATC TTCCACTCTT
     1021 AAATCACTGG AGGAGAAAGA CCATATTCAC CGTGTCCTGG ACAAGATCAC AGATACTCTT
     1081 ATCCATCTTA TGGCGAAGGC AGGCTTAACA CTTCAACAGC AACATCAGCG CCTTGCTCAG
     1141 CTTCTGTTGA TTTTAAGCCA TATCCGTCAC ATGTCAAACA AGGGTATGGA ACACCTTTAC
     1201 AGTATGAAGT GTAAGAACGT CGTTCCATTG TATGATTTGT TACTGGAAAT GCTTGACGCT
     1261 CACCGTCTGC ATGCACCTGG TGGCGGTGGC TCTGGTGGAG GCGGGAGCGC AGCTgCAGCT
     1321 GAAGAAGCTG GTATAGACTT ACCGTATTCT TGTCGTGCTG GTGCTTGCTC TACCTGTGCT
     1381 GGTAAACTCA TATCCGGTAC TGTCGATCAA AGCGATCAGT CATTTTTAGA CGACGATCAA
     1441 ATTGAAGCTG GATACGTTCT CACCTGCGTT GCTTACCCAA CTTCCGATTG CGTCATCGAA
     1501 ACACACAAAG AAGAAGAGCT TTACTAAgaa ttcTGATAAT AATCTAGACC AGGCATCAAA
     1561 TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA
     1621 ACGCTCTCTA CTAGAGTCAC ACTGGCTCAC CTTCGGGTGG GCCTTTCTGC GTTTATAGGT
     1681 ACCCGTGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTTTTACGGC TAGCTCAGTC
     1741 CTAGGTATAG TGCTAGCCCA GCCAGAGAAA CACTCTTTAA CAGGGGGTAG TATGATGTCT
     1801 CGTTTAGATA AAAGTAAAGT GATTAACAGC GCATTAGAGC TGCTTAATGA GGTCGGAATC
     1861 GAAGGTTTAA CAACCCGTAA ACTCGCCCAG AAGCTAGGTG TAGAGCAGCC TACATTGTAT
     1921 TGGCATGTAA AAAATAAGCG GGCTTTGCTC GACGCCTTAG CCATTGAGAT GTTAGATAGG
     1981 CACCATACTC ACTTTTGCCC TTTAGAAGGG GAAAGCTGGC AAGATTTTTT ACGTAATAAC
     2041 GCTAAAAGTT TTAGATGTGC TTTACTAAGT CATCGCGATG GAGCAAAAGT ACATTTAGGT
     2101 ACACGGCCTA CAGAAAAACA GTATGAAACT CTCGAAAATC AATTAGCCTT TTTATGCCAA
     2161 CAAGGTTTTT CACTAGAGAA TGCATTATAT GCACTCAGCG CAGTGGGGCA TTTTACTTTA
     2221 GGTTGCGTAT TGGAAGATCA AGAGCATCAA GTCGCTAAAG AAGAAAGGGA AACACCTACT
     2281 ACTGATAGTA TGCCGCCATT ATTACGACAA GCTATCGAAT TATTTGATCA CCAAGGTGCA
     2341 GAGCCAGCCT TCTTATTCGG CCTTGAATTG ATCATATGCG GATTAGAAAA ACAACTTAAA
     2401 TGTGAAAGTG GGTCTTAAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT
     2461 TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGCCCT
     2521 AGACCTAGGC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     2581 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     2641 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     2701 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     2761 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     2821 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG
     2881 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     2941 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     3001 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     3061 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
     3121 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     3181 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     3241 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     3301 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGACT AGTGCTTGGA TTCTCACCAA
     3361 TAAAAAACGC CCGGCGGCAA CCGAGCGTTC TGAACAAATC CAGATGGAGT TCTGAGGTCA
     3421 TTACTGGATC TATCAACAGG AGTCCAAGCG AGCTCGATAT CAAATTACGC CCCGCCCTGC
     3481 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAG
     3541 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT
     3601 TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA
     3661 CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA
     3721 GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC
     3781 TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG
     3841 AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC
     3901 ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA
     3961 AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC
     4021 TGGTTATAGG TACATTGAGC AACTGA
//